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pAML10

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01949
Resource name pAML10
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), centromere-type ori, LEU2, ADH1 promoter, Ampicillin
Vector backbone pAML10 (Plasmid)
Size of vector backbone 5,124 bp
Selectable markers Amp^r (E. coli), LEU2 (S. cerevisiae)
Depositor|Developer Iha, Hidekatsu |
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External Database

            Reference sequence
              

            Distribution information

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            RDB01949 pAML10 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB01949z.seq
            Remarks, protocol and/or map (gif) RDB01949.gif
            Remarks, protocol and/or map (gif) RDB01949z.gif

            Original reference

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13043_A4Lj.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADHrev
            Sequence file: RDB13043_A4Lja.seq check
            >01949_13043_A4Lj_ADHrev_B09_06.ab1
                1 AGAAATACTC AAGATAAGAA TTTTCGTTTT AAACCTAAGA GTCACTTTAA AATTTGTATA
               61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCCC
              121 GGAATTAGCT TGGCTGCAGG TCGACTCACA GATCCTCTTC GGAAATCAGT TTCTGTTCAG
              181 AATTCGAGCT CGGTACCCGG GGATCCTCTA GAGTCAGCTT GGAGTTGATT GTATGCTTGG
              241 TATAGCTTGA AATATTGTGC AGAAAAAGAA ACAAGGAAGA AAGGGAACGA GAACAATGAC
              301 GAGGAAACAA AAGATTAATA ATTGCAGGTC TATTTATACT TGATAGCAAG ACAGCAAACT
              361 TTTTTTTATT TCAAATTCAA GTAACTGGAA GGAAGGCCGT ATACCGTTGC TCATTAGAGA
              421 GTAGTGTGCG TGAATGAAGG AAGGAAAAAG TTTCGTGTGC TTCGAGATAC CCCTCATCAG
              481 CTCTGGAACA ACGACATCTG TTGGTGCTGT CTTTGTCGTT AATTTTTTCC TTTAGTGTCT
              541 TCCATCATTT TTTTGTCATT GCGGATATGG TGAGACAACA ACGGGGGAGA GAGAAAAGAA
              601 AAAAAAAGAA AAGAAGTTGC ATGCAAGCTC TGCATGTGTC AGAGGTTTTC ACCGTCATCA
              661 CCGAAACGCG CGAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG
              721 ATAATAATGG TTTCTTAGGG TCCTTTTCAT CACGTGCTAT AAAAATAATT ATAATTTAAA
              781 TTTTTTAATA TAAATATATA AATTAAAAAT AGAAAGTAAA AAAAGAAATT AAAGAAAAAA
              841 TAGTTTTTGT TTTCCGAAGA TGTAAAAGAC TCTAGGGGGA TCGCCAACAA ATACTACCTT
              901 TTATCTTGCT CTTCCTGCTC TCAGGTATTA ATGCCGAATT GTTTCATCTT GTCTGTGTAG
              961 AAGACCACAC ACGAAAATCC TGTGATTTTA CATTTTACTT ATCGTTAATC GAATGTATAT
             1021 CTATTTAAAT CTGCTTTTCT TGTCTAATAA ATATATATGT AAAGTACGCT TTTTGTTGGA
             1081 AATTTTTAAA CCTTTGATTA TTTTTTTTCT TCATCCGTAA CTCTTCCTAC CTTCGTTTAA
            1141 TTTTAACCTT TTCCTTAAAA AAAAAAT
            //
            Primer: Amp_R
            Sequence file: RDB13043_A4Ljb.seq check
            >01949_13043_A4Lj_Amp_R_C09_09.ab1
                1 TTCTTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
               61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
              121 CTGACGGATC GCTTGCCTGT AACTTACACG CGCCTCGTAT CTTTTAATGA TGGAATAATT
              181 TGGGAATTTA CTCTGTGTTT ATTTATTTTT ATGTTTTGTA TTTGGATTTT AGAAAGTAAA
              241 TAAAGAAGGT AGAAGAGTTA CGGAATGAAG AAAAAAAAAT AAACAAAGGT TTAAAAAATT
              301 TCAACAAAAA GCGTACTTTA CATATATATT TATTAGACAA GAAAAGCAGA TTAAATAGAT
              361 ATACATTCGA TTAACGATAA GTAAAATGTA AAATCACAGG ATTTTCGTGT GTGGTCTTCT
              421 ACACAGACAA GATGAAACAA TTCGGCATTA ATACCTGAGA GCAGGAAGAG CAAGATAAAA
              481 GGTAGTATTT GTTGGCGATC CCCCTAGAGT CTTTTACATC TTCGGAAAAC AAAAACTATT
              541 TTTTCTTTAA TTTCTTTTTT TACTTTCTAT TTTTAATTTA TATATTTATA TTAAAAAATT
              601 TAAATTATAA TTATTTTTAT AGCACGTGAT GAAAAGGACC CTAAGAAACC ATTATTATCA
              661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
              721 ATGACGGTGA AAACCTCTGA CACATGCAGA GCTTGCATGC AACTTCTTTT CTTTTTTTTT
              781 CTTTTCTCTC TCCCCCGTTG TTGTCTCACC ATATCCGCAA TGACAAAAAA ATGATGGAAG
              841 ACACTAAAGG AAAAAATTAA CGACAAAGAC AGCACCAACA GATGTCGTTG TTCCAGAGCT
              901 GATGAGGGGT ATCTCGAAGC ACACGAAACT TTTTCCTTCC TTCATTCACG CACACTACTC
              961 TCTAATGAGC AACGGTATAC GGGCCTTCCT TCCAGTTACT TGAATTTGAA ATAAAAAAAA
             1021 AGTTTGCTGT CTTGCTATCA AGTATAAATA GACCCTGCAT TATTAATCTT TTGTTTCCTC
             1081 GTCATGATCT CGTTCCCTTT TCTTCCCTTT GCTCCTTTTC CTGCACATAT TTTCAGCTAT
            1141 ACCAAGCCAA TAACAATTCC AACTTCCCAA AG
            //
            Primer: LEU2pro_F
            Sequence file: RDB13043_A4Ljc.seq check
            >01949_13043_A4Lj_LEU2pro_F_D09_12.ab1
                1 AATATGCAGC TGCATATTAT TTAGGACCTA TTGTTTTTTC CAATAGGTGG TTAGCAATCG
               61 TCTTACTTTC TAACTTTTCT TACCTTTTAC ATTTCAGCAA TATATATATA TATATTTCAA
              121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT
              181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC
              241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT
              301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT
              361 GCTGTGGGTG GTCCTAAATG GGGTACAGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA
              421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT
              481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC
              541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC
              601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT
              661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT
              721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTTCCT
              781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA
              841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGATATCAT CTCCGATGAA
              901 GCCTCCGTTA TCCCAGGTTC CTTGGGTTTG TTGCCATCTG CGTCCTTGGC CTCTTTGCCA
              961 GACAAGAACA CCGCATTTGG TTTGTACGAA CCCATGCCAC GGGTTCTGCT CCAGATTTGC
             1021 CCAAAGAATA ACGTCAACCC CTATCGCCAC TATCTTGTCT GCTGCAATGA TGTGAAATTG
             1081 TCATTTGAAC TGCTGAGAGT AACGCCATTG AGATGCAGTA AAAGATTTTG AATGCAGGTA
            1141 TCAGAACTGG GGTGAATTTT AAGGGTTGGC TC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl