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pAML10

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01949
Resource name pAML10
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), centromere-type ori, LEU2, ADH1 promoter, Ampicillin
Vector backbone pAML10 (Plasmid)
Size of vector backbone 5,124 bp
Selectable markers Amp^r (E. coli), LEU2 (S. cerevisiae)
Depositor Iha, Hidekatsu |

Distribution information

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RDB01949 pAML10 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB01949z.seq from Depositor
Electronic file Map RDB01949z.gif from Depositor
Electronic file Map RDB01949.gif from Depositor

Featured content

Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13043_A4Lj.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev RDB13043_A4Lja.seq
>01949_13043_A4Lj_ADHrev_B09_06.ab1
    1 AGAAATACTC AAGATAAGAA TTTTCGTTTT AAACCTAAGA GTCACTTTAA AATTTGTATA
   61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCCC
  121 GGAATTAGCT TGGCTGCAGG TCGACTCACA GATCCTCTTC GGAAATCAGT TTCTGTTCAG
  181 AATTCGAGCT CGGTACCCGG GGATCCTCTA GAGTCAGCTT GGAGTTGATT GTATGCTTGG
  241 TATAGCTTGA AATATTGTGC AGAAAAAGAA ACAAGGAAGA AAGGGAACGA GAACAATGAC
  301 GAGGAAACAA AAGATTAATA ATTGCAGGTC TATTTATACT TGATAGCAAG ACAGCAAACT
  361 TTTTTTTATT TCAAATTCAA GTAACTGGAA GGAAGGCCGT ATACCGTTGC TCATTAGAGA
  421 GTAGTGTGCG TGAATGAAGG AAGGAAAAAG TTTCGTGTGC TTCGAGATAC CCCTCATCAG
  481 CTCTGGAACA ACGACATCTG TTGGTGCTGT CTTTGTCGTT AATTTTTTCC TTTAGTGTCT
  541 TCCATCATTT TTTTGTCATT GCGGATATGG TGAGACAACA ACGGGGGAGA GAGAAAAGAA
  601 AAAAAAAGAA AAGAAGTTGC ATGCAAGCTC TGCATGTGTC AGAGGTTTTC ACCGTCATCA
  661 CCGAAACGCG CGAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG
  721 ATAATAATGG TTTCTTAGGG TCCTTTTCAT CACGTGCTAT AAAAATAATT ATAATTTAAA
  781 TTTTTTAATA TAAATATATA AATTAAAAAT AGAAAGTAAA AAAAGAAATT AAAGAAAAAA
  841 TAGTTTTTGT TTTCCGAAGA TGTAAAAGAC TCTAGGGGGA TCGCCAACAA ATACTACCTT
  901 TTATCTTGCT CTTCCTGCTC TCAGGTATTA ATGCCGAATT GTTTCATCTT GTCTGTGTAG
  961 AAGACCACAC ACGAAAATCC TGTGATTTTA CATTTTACTT ATCGTTAATC GAATGTATAT
 1021 CTATTTAAAT CTGCTTTTCT TGTCTAATAA ATATATATGT AAAGTACGCT TTTTGTTGGA
 1081 AATTTTTAAA CCTTTGATTA TTTTTTTTCT TCATCCGTAA CTCTTCCTAC CTTCGTTTAA
1141 TTTTAACCTT TTCCTTAAAA AAAAAAT
//
Primer: Amp_R RDB13043_A4Ljb.seq
>01949_13043_A4Lj_Amp_R_C09_09.ab1
    1 TTCTTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGACGGATC GCTTGCCTGT AACTTACACG CGCCTCGTAT CTTTTAATGA TGGAATAATT
  181 TGGGAATTTA CTCTGTGTTT ATTTATTTTT ATGTTTTGTA TTTGGATTTT AGAAAGTAAA
  241 TAAAGAAGGT AGAAGAGTTA CGGAATGAAG AAAAAAAAAT AAACAAAGGT TTAAAAAATT
  301 TCAACAAAAA GCGTACTTTA CATATATATT TATTAGACAA GAAAAGCAGA TTAAATAGAT
  361 ATACATTCGA TTAACGATAA GTAAAATGTA AAATCACAGG ATTTTCGTGT GTGGTCTTCT
  421 ACACAGACAA GATGAAACAA TTCGGCATTA ATACCTGAGA GCAGGAAGAG CAAGATAAAA
  481 GGTAGTATTT GTTGGCGATC CCCCTAGAGT CTTTTACATC TTCGGAAAAC AAAAACTATT
  541 TTTTCTTTAA TTTCTTTTTT TACTTTCTAT TTTTAATTTA TATATTTATA TTAAAAAATT
  601 TAAATTATAA TTATTTTTAT AGCACGTGAT GAAAAGGACC CTAAGAAACC ATTATTATCA
  661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
  721 ATGACGGTGA AAACCTCTGA CACATGCAGA GCTTGCATGC AACTTCTTTT CTTTTTTTTT
  781 CTTTTCTCTC TCCCCCGTTG TTGTCTCACC ATATCCGCAA TGACAAAAAA ATGATGGAAG
  841 ACACTAAAGG AAAAAATTAA CGACAAAGAC AGCACCAACA GATGTCGTTG TTCCAGAGCT
  901 GATGAGGGGT ATCTCGAAGC ACACGAAACT TTTTCCTTCC TTCATTCACG CACACTACTC
  961 TCTAATGAGC AACGGTATAC GGGCCTTCCT TCCAGTTACT TGAATTTGAA ATAAAAAAAA
 1021 AGTTTGCTGT CTTGCTATCA AGTATAAATA GACCCTGCAT TATTAATCTT TTGTTTCCTC
 1081 GTCATGATCT CGTTCCCTTT TCTTCCCTTT GCTCCTTTTC CTGCACATAT TTTCAGCTAT
1141 ACCAAGCCAA TAACAATTCC AACTTCCCAA AG
//
Primer: LEU2pro_F RDB13043_A4Ljc.seq
>01949_13043_A4Lj_LEU2pro_F_D09_12.ab1
    1 AATATGCAGC TGCATATTAT TTAGGACCTA TTGTTTTTTC CAATAGGTGG TTAGCAATCG
   61 TCTTACTTTC TAACTTTTCT TACCTTTTAC ATTTCAGCAA TATATATATA TATATTTCAA
  121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT
  181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC
  241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT
  301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT
  361 GCTGTGGGTG GTCCTAAATG GGGTACAGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA
  421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT
  481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC
  541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC
  601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT
  661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT
  721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTTCCT
  781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA
  841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGATATCAT CTCCGATGAA
  901 GCCTCCGTTA TCCCAGGTTC CTTGGGTTTG TTGCCATCTG CGTCCTTGGC CTCTTTGCCA
  961 GACAAGAACA CCGCATTTGG TTTGTACGAA CCCATGCCAC GGGTTCTGCT CCAGATTTGC
 1021 CCAAAGAATA ACGTCAACCC CTATCGCCAC TATCTTGTCT GCTGCAATGA TGTGAAATTG
 1081 TCATTTGAAC TGCTGAGAGT AACGCCATTG AGATGCAGTA AAAGATTTTG AATGCAGGTA
1141 TCAGAACTGG GGTGAATTTT AAGGGTTGGC TC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

2018.12.11

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