RDB01948 - pAMH20

Resource data sheet
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pAMH20

E. coli - S. cerevisiae shuttle vector

Catalog number	RDB01948
Resource name	pAMH20
Alternative name	E. coli-S. cerevisiae shuttle vector
Clone info.	E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), 2micron ori, HIS3, ADH1 promoter, Ampicillin
Vector backbone	pAMH20 (Plasmid)
Size of vector backbone	5,287 bp
Selectable markers	Amp^r (E. coli), HIS3 (S. cerevisiae)
Depositor	Iha, Hidekatsu \|

Distribution information

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01948	pAMH20	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB01948z.seq from Depositor
Electronic file	Map RDB01948z.gif from Depositor
Electronic file	Map RDB01948.gif from Depositor

Featured content	Expression Vector Backbone of Saccharomyces cerevisiae (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB13121_A5Bg.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH_rev	RDB13121_A5Bga.seq
>01948_13121_A5Bg_ADHrev_A10_01.ab1 1 GCAAGATACT CAGAATAAGA ATTTTCGTTT TAAACCTAAG AGTCACTTTA AAATTTGTAT 61 ACACTTATTT TTTTTATAAC TTATTTAATA ATAAAAATCA TAAATCATAA GAAATTCGCC 121 CGGAATTAGC TTGGCTGCAG GTCGACTCAC AGATCCTCTT CGGAAATCAG TTTCTGTTCA 181 GAATTCGAGC TCGGTACCCG GGGATCCTCT AGAGTCAGCT TGGAGTTGAT TGTATGCTTG 241 GTATAGCTTG AAATATTGTG CAGAAAAAGA AACAAGGAAG AAAGGGAACG AGAACAATGA 301 CGAGGAAACA AAAGATTAAT AATTGCAGGT CTATTTATAC TTGATAGCAA GACAGCAAAC 361 TTTTTTTTAT TTCAAATTCA AGTAACTGGA AGGAAGGCCG TATACCGTTG CTCATTAGAG 421 AGTAGTGTGC GTGAATGAAG GAAGGAAAAA GTTTCGTGTG CTTCGAGATA CCCCTCATCA 481 GCTCTGGAAC AACGACATCT GTTGGTGCTG TCTTTGTCGT TAATTTTTTC CTTTAGTGTC 541 TTCCATCATT TTTTTGTCAT TGCGGATATG GTGAGACAAC AACGGGGGAG AGAGAAAAGA 601 AAAAAAAAGA AAAGAAGTTG CATGCAAGCA ACGAAGCATC TGTGCTTCAT TTTGTAGAAC 661 AAAAATGCAA CGCGAGAGCG CTAATTTTTC AAACAAAGAA TCTGAGCTGC ATTTTTACAG 721 AACAGAAATG CAACGCGAAA GCGCTATTTT ACCAACGAAG AATCTGTGCT TCATTTTTGT 781 AAAACAAAAA TGCAACGCGA GAGCGCTAAT TTTTCAAACA AAGAATCTGA GCTGCATTTT 841 TACAGAACAG AAATGCAACG CGAGAGCGCT ATTTTACCAA CAAAGAATCT ATACTTCTTT 901 TTTTGTTCTA CAAAAATGCA TCCCGAGAGC GCTATTTTTT CTAACAAAGC ATCTTTAGAT 961 TACTTTTTTT TCTCCCTTTG TGCGCTCTAT AATGCAGTCT CTTTGATAAC TTTTTGCACT 1021 GTAGGTCCCG TTAGATAGAG AGCTACCTTT GGTGTCTATT TTCTCTCATA AAAAAGCCTG 1081 ACTCCACATC CCGCGATTTA CTGAATAACC TTAGCCGAAG GCG //
Primer: Amp_R	RDB13121_A5Bgb.seq
>01948_13121_A5Bg_Amp_R_B10_04.ab1 1 TCATTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA 181 GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC 241 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG 301 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG 361 TACTGAGAGT GCACCATAAC GCATTTAAGC ATAAACACGC ACTATGCCGT TCTTCTCATG 421 TATATATATA TACAGGCAAC ACGCAGATAT AGGTGCGACG TGAACAGTGA GCTGTATGTG 481 CGCAGCTCGC GTTGCATTTT CGGAAGCGCT CGTTTTCGGA AACGCTTTGA AGTTCCTATT 541 CCGAAGTTCC TATTCTCTAG CTAGAAAGTA TAGGAACTTC AGAGCGCTTT TGAAAACCAA 601 AAGCGCTCTG AAGACGCACT TTCAAAAAAC CAAAAACGCA CCGGACTGTA ACGAGCTACT 661 AAAATATTGC GAATACCGCT TCCACAAACA TTGCTCAAAA GTATCTCTTT GCTATATATC 721 TCTGTGCTAT ATCCCTATAT AACCTACCCA TCCACCTTTC GCTCCTTGAA CTTGCATCTA 781 AACTCGACCT CTACATTTTT TATGTTTATC TCTAGTATTA CTCTTTAGAC AAAAAAAATT 841 GTAGTAAGAA CTATTCATAG AGTGAATCGA AAACAATACG AAAATGTAAA CATTTTCCTA 901 TACGTAGTAT ATAGAGACAA AATAGAAGAA ACCGTTCATA ATTTTCTGAC CAATGAAGAA 961 TCATCAACGC TATCACTTTC TGTTCACAAA GTATGCGCAA TCCACATCGG TATAGAATAT 1021 AATCGGGGAT GCCCTTTATC TTGAAAAAAA TGCCACCGCC AGCCTTCGCT AGGTAATTCA 1081 GTTAAACGCG GGAAGGTTGG AAGTCAGGCT TTTTTATGAA AGAGAAGATT AGGAACGTCA 1141 AAAAGTAAGG CCATTC //
Primer: HIS3pro_F	RDB13121_A5Bgc.seq
>01948_13121_A5Bg_HIS3pro_F_C10_07.ab1 1 TGGCAATATA CATTATATAA AGTAATGTGA TTTCTTCGAA GAATATACTA AAAAATGAGC 61 AGGCAAGATA AACGAAGGCA AAGATGACAG AGCAGAAAGC CCTAGTAAAG CGTATTACAA 121 ATGAAACCAA GATTCAGATT GCGATCTCTT TAAAGGGTGG TCCCCTAGCG ATAGAGCACT 181 CGATCTTCCC AGAAAAAGAG GCAGAAGCAG TAGCAGAACA GGCCACACAA TCGCAAGTGA 241 TTAACGTCCA CACAGGTATA GGGTTTCTGG ACCATATGAT ACATGCTCTG GCCAAGCATT 301 CCGGCTGGTC GCTAATCGTT GAGTGCATTG GTGACTTACA CATAGACGAC CATCACACCA 361 CTGAAGACTG CGGGATTGCT CTCGGTCAAG CATTTAAAGA GGCCCTAGGG GCCGTGCGTG 421 GAGTAAAAAG GTTTGGATCA GGATTTGCGC CTTTGGATGA GGCACTTTCC AGAGCGGTGG 481 TAGATCTTTC GAACAGGCCG TACGCAGTTG TCGAACTTGG TTTGCAGAGG GAGAAAGTAG 541 GAGATCTCTC TTGCGAGATG ATCCCGCATT TTCTTGAAAG TTTTGCAGAG GCAAGCAGAA 601 TTACCCTCCA CGTTGATTGT CTGCGAGGCA AGAATGATCA TCACCGTAGT GAGAGTGCGT 661 TCAAGGCTCT TGCGGTTGCC ATAAGAGAAG CCACCTCGCC CAATGGAACC AACGATGTTC 721 CCTCCACCAA AGGTGTTCTT ATGTAGTGAC ACCGATTATT TAAAGCGGCA AGCTTACATT 781 TTATGTTAGC TGGTGGACTG ACGCCAGAAA ATGTTGGTGA TGCGCTTAGA TTAAATGGCG 841 TTATTGGTGT TGATGTAAGC GGAGGTGTGG AGACAAATGG TGTAAAAGAC TCTAACAAAA 901 TAGCAAATTT CGTCAAAAAT GCTAAGAAAT AGGTTATTAC TGAGTAGTAT TTATTTAAGT 961 ATTTGTTTGT GCACTTGCCC GGTAGAGGTG TGGTCAATAA GAGCGACCTC ATGCTATACC 1021 CTGAGAAAGC AACCCTGACC CTACAGGAAA GAGTTTACTC AAGAATAAGA TTTTCGTTTT 1081 AAAACCTAAG AGTTCACTTT AAAATTTGTA TAACACTTAC TTTTTTTGAT GACTTAGTCA 1141 TATAGAGATT CATTTAGAAG TTA //

Please visit Sequencing and PCR primers for primer information.

References

original	Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl