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pAMH20

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01948
Resource name pAMH20
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), 2micron ori, HIS3, ADH1 promoter, Ampicillin
Vector backbone pAMH20 (Plasmid)
Size of vector backbone 5,287 bp
Selectable markers Amp^r (E. coli), HIS3 (S. cerevisiae)
Depositor|Developer Iha, Hidekatsu |
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External Database

            Reference sequence
              

            Distribution information

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            RDB01948 pAMH20 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB01948z.seq
            Remarks, protocol and/or map (gif) RDB01948.gif
            Remarks, protocol and/or map (gif) RDB01948z.gif

            Original reference

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13121_A5Bg.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADH_rev
            Sequence file: RDB13121_A5Bga.seq check
            >01948_13121_A5Bg_ADHrev_A10_01.ab1
                1 GCAAGATACT CAGAATAAGA ATTTTCGTTT TAAACCTAAG AGTCACTTTA AAATTTGTAT
               61 ACACTTATTT TTTTTATAAC TTATTTAATA ATAAAAATCA TAAATCATAA GAAATTCGCC
              121 CGGAATTAGC TTGGCTGCAG GTCGACTCAC AGATCCTCTT CGGAAATCAG TTTCTGTTCA
              181 GAATTCGAGC TCGGTACCCG GGGATCCTCT AGAGTCAGCT TGGAGTTGAT TGTATGCTTG
              241 GTATAGCTTG AAATATTGTG CAGAAAAAGA AACAAGGAAG AAAGGGAACG AGAACAATGA
              301 CGAGGAAACA AAAGATTAAT AATTGCAGGT CTATTTATAC TTGATAGCAA GACAGCAAAC
              361 TTTTTTTTAT TTCAAATTCA AGTAACTGGA AGGAAGGCCG TATACCGTTG CTCATTAGAG
              421 AGTAGTGTGC GTGAATGAAG GAAGGAAAAA GTTTCGTGTG CTTCGAGATA CCCCTCATCA
              481 GCTCTGGAAC AACGACATCT GTTGGTGCTG TCTTTGTCGT TAATTTTTTC CTTTAGTGTC
              541 TTCCATCATT TTTTTGTCAT TGCGGATATG GTGAGACAAC AACGGGGGAG AGAGAAAAGA
              601 AAAAAAAAGA AAAGAAGTTG CATGCAAGCA ACGAAGCATC TGTGCTTCAT TTTGTAGAAC
              661 AAAAATGCAA CGCGAGAGCG CTAATTTTTC AAACAAAGAA TCTGAGCTGC ATTTTTACAG
              721 AACAGAAATG CAACGCGAAA GCGCTATTTT ACCAACGAAG AATCTGTGCT TCATTTTTGT
              781 AAAACAAAAA TGCAACGCGA GAGCGCTAAT TTTTCAAACA AAGAATCTGA GCTGCATTTT
              841 TACAGAACAG AAATGCAACG CGAGAGCGCT ATTTTACCAA CAAAGAATCT ATACTTCTTT
              901 TTTTGTTCTA CAAAAATGCA TCCCGAGAGC GCTATTTTTT CTAACAAAGC ATCTTTAGAT
              961 TACTTTTTTT TCTCCCTTTG TGCGCTCTAT AATGCAGTCT CTTTGATAAC TTTTTGCACT
             1021 GTAGGTCCCG TTAGATAGAG AGCTACCTTT GGTGTCTATT TTCTCTCATA AAAAAGCCTG
            1081 ACTCCACATC CCGCGATTTA CTGAATAACC TTAGCCGAAG GCG
            //
            Primer: Amp_R
            Sequence file: RDB13121_A5Bgb.seq check
            >01948_13121_A5Bg_Amp_R_B10_04.ab1
                1 TCATTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
               61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
              121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
              181 GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC
              241 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG
              301 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG
              361 TACTGAGAGT GCACCATAAC GCATTTAAGC ATAAACACGC ACTATGCCGT TCTTCTCATG
              421 TATATATATA TACAGGCAAC ACGCAGATAT AGGTGCGACG TGAACAGTGA GCTGTATGTG
              481 CGCAGCTCGC GTTGCATTTT CGGAAGCGCT CGTTTTCGGA AACGCTTTGA AGTTCCTATT
              541 CCGAAGTTCC TATTCTCTAG CTAGAAAGTA TAGGAACTTC AGAGCGCTTT TGAAAACCAA
              601 AAGCGCTCTG AAGACGCACT TTCAAAAAAC CAAAAACGCA CCGGACTGTA ACGAGCTACT
              661 AAAATATTGC GAATACCGCT TCCACAAACA TTGCTCAAAA GTATCTCTTT GCTATATATC
              721 TCTGTGCTAT ATCCCTATAT AACCTACCCA TCCACCTTTC GCTCCTTGAA CTTGCATCTA
              781 AACTCGACCT CTACATTTTT TATGTTTATC TCTAGTATTA CTCTTTAGAC AAAAAAAATT
              841 GTAGTAAGAA CTATTCATAG AGTGAATCGA AAACAATACG AAAATGTAAA CATTTTCCTA
              901 TACGTAGTAT ATAGAGACAA AATAGAAGAA ACCGTTCATA ATTTTCTGAC CAATGAAGAA
              961 TCATCAACGC TATCACTTTC TGTTCACAAA GTATGCGCAA TCCACATCGG TATAGAATAT
             1021 AATCGGGGAT GCCCTTTATC TTGAAAAAAA TGCCACCGCC AGCCTTCGCT AGGTAATTCA
             1081 GTTAAACGCG GGAAGGTTGG AAGTCAGGCT TTTTTATGAA AGAGAAGATT AGGAACGTCA
            1141 AAAAGTAAGG CCATTC
            //
            Primer: HIS3pro_F
            Sequence file: RDB13121_A5Bgc.seq check
            >01948_13121_A5Bg_HIS3pro_F_C10_07.ab1
                1 TGGCAATATA CATTATATAA AGTAATGTGA TTTCTTCGAA GAATATACTA AAAAATGAGC
               61 AGGCAAGATA AACGAAGGCA AAGATGACAG AGCAGAAAGC CCTAGTAAAG CGTATTACAA
              121 ATGAAACCAA GATTCAGATT GCGATCTCTT TAAAGGGTGG TCCCCTAGCG ATAGAGCACT
              181 CGATCTTCCC AGAAAAAGAG GCAGAAGCAG TAGCAGAACA GGCCACACAA TCGCAAGTGA
              241 TTAACGTCCA CACAGGTATA GGGTTTCTGG ACCATATGAT ACATGCTCTG GCCAAGCATT
              301 CCGGCTGGTC GCTAATCGTT GAGTGCATTG GTGACTTACA CATAGACGAC CATCACACCA
              361 CTGAAGACTG CGGGATTGCT CTCGGTCAAG CATTTAAAGA GGCCCTAGGG GCCGTGCGTG
              421 GAGTAAAAAG GTTTGGATCA GGATTTGCGC CTTTGGATGA GGCACTTTCC AGAGCGGTGG
              481 TAGATCTTTC GAACAGGCCG TACGCAGTTG TCGAACTTGG TTTGCAGAGG GAGAAAGTAG
              541 GAGATCTCTC TTGCGAGATG ATCCCGCATT TTCTTGAAAG TTTTGCAGAG GCAAGCAGAA
              601 TTACCCTCCA CGTTGATTGT CTGCGAGGCA AGAATGATCA TCACCGTAGT GAGAGTGCGT
              661 TCAAGGCTCT TGCGGTTGCC ATAAGAGAAG CCACCTCGCC CAATGGAACC AACGATGTTC
              721 CCTCCACCAA AGGTGTTCTT ATGTAGTGAC ACCGATTATT TAAAGCGGCA AGCTTACATT
              781 TTATGTTAGC TGGTGGACTG ACGCCAGAAA ATGTTGGTGA TGCGCTTAGA TTAAATGGCG
              841 TTATTGGTGT TGATGTAAGC GGAGGTGTGG AGACAAATGG TGTAAAAGAC TCTAACAAAA
              901 TAGCAAATTT CGTCAAAAAT GCTAAGAAAT AGGTTATTAC TGAGTAGTAT TTATTTAAGT
              961 ATTTGTTTGT GCACTTGCCC GGTAGAGGTG TGGTCAATAA GAGCGACCTC ATGCTATACC
             1021 CTGAGAAAGC AACCCTGACC CTACAGGAAA GAGTTTACTC AAGAATAAGA TTTTCGTTTT
             1081 AAAACCTAAG AGTTCACTTT AAAATTTGTA TAACACTTAC TTTTTTTGAT GACTTAGTCA
            1141 TATAGAGATT CATTTAGAAG TTA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl