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pAMH20

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01948
Resource name pAMH20
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), 2micron ori, HIS3, ADH1 promoter, Ampicillin
Vector backbone pAMH20 (Plasmid)
Size of vector backbone 5,287 bp
Selectable markers Amp^r (E. coli), HIS3 (S. cerevisiae)
Depositor Iha, Hidekatsu |

Distribution information

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RDB01948 pAMH20 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB01948z.seq from Depositor
Electronic file Map RDB01948z.gif from Depositor
Electronic file Map RDB01948.gif from Depositor

Featured content

Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13121_A5Bg.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH_rev RDB13121_A5Bga.seq
>01948_13121_A5Bg_ADHrev_A10_01.ab1
    1 GCAAGATACT CAGAATAAGA ATTTTCGTTT TAAACCTAAG AGTCACTTTA AAATTTGTAT
   61 ACACTTATTT TTTTTATAAC TTATTTAATA ATAAAAATCA TAAATCATAA GAAATTCGCC
  121 CGGAATTAGC TTGGCTGCAG GTCGACTCAC AGATCCTCTT CGGAAATCAG TTTCTGTTCA
  181 GAATTCGAGC TCGGTACCCG GGGATCCTCT AGAGTCAGCT TGGAGTTGAT TGTATGCTTG
  241 GTATAGCTTG AAATATTGTG CAGAAAAAGA AACAAGGAAG AAAGGGAACG AGAACAATGA
  301 CGAGGAAACA AAAGATTAAT AATTGCAGGT CTATTTATAC TTGATAGCAA GACAGCAAAC
  361 TTTTTTTTAT TTCAAATTCA AGTAACTGGA AGGAAGGCCG TATACCGTTG CTCATTAGAG
  421 AGTAGTGTGC GTGAATGAAG GAAGGAAAAA GTTTCGTGTG CTTCGAGATA CCCCTCATCA
  481 GCTCTGGAAC AACGACATCT GTTGGTGCTG TCTTTGTCGT TAATTTTTTC CTTTAGTGTC
  541 TTCCATCATT TTTTTGTCAT TGCGGATATG GTGAGACAAC AACGGGGGAG AGAGAAAAGA
  601 AAAAAAAAGA AAAGAAGTTG CATGCAAGCA ACGAAGCATC TGTGCTTCAT TTTGTAGAAC
  661 AAAAATGCAA CGCGAGAGCG CTAATTTTTC AAACAAAGAA TCTGAGCTGC ATTTTTACAG
  721 AACAGAAATG CAACGCGAAA GCGCTATTTT ACCAACGAAG AATCTGTGCT TCATTTTTGT
  781 AAAACAAAAA TGCAACGCGA GAGCGCTAAT TTTTCAAACA AAGAATCTGA GCTGCATTTT
  841 TACAGAACAG AAATGCAACG CGAGAGCGCT ATTTTACCAA CAAAGAATCT ATACTTCTTT
  901 TTTTGTTCTA CAAAAATGCA TCCCGAGAGC GCTATTTTTT CTAACAAAGC ATCTTTAGAT
  961 TACTTTTTTT TCTCCCTTTG TGCGCTCTAT AATGCAGTCT CTTTGATAAC TTTTTGCACT
 1021 GTAGGTCCCG TTAGATAGAG AGCTACCTTT GGTGTCTATT TTCTCTCATA AAAAAGCCTG
1081 ACTCCACATC CCGCGATTTA CTGAATAACC TTAGCCGAAG GCG
//
Primer: Amp_R RDB13121_A5Bgb.seq
>01948_13121_A5Bg_Amp_R_B10_04.ab1
    1 TCATTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
  181 GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC
  241 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG
  301 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG
  361 TACTGAGAGT GCACCATAAC GCATTTAAGC ATAAACACGC ACTATGCCGT TCTTCTCATG
  421 TATATATATA TACAGGCAAC ACGCAGATAT AGGTGCGACG TGAACAGTGA GCTGTATGTG
  481 CGCAGCTCGC GTTGCATTTT CGGAAGCGCT CGTTTTCGGA AACGCTTTGA AGTTCCTATT
  541 CCGAAGTTCC TATTCTCTAG CTAGAAAGTA TAGGAACTTC AGAGCGCTTT TGAAAACCAA
  601 AAGCGCTCTG AAGACGCACT TTCAAAAAAC CAAAAACGCA CCGGACTGTA ACGAGCTACT
  661 AAAATATTGC GAATACCGCT TCCACAAACA TTGCTCAAAA GTATCTCTTT GCTATATATC
  721 TCTGTGCTAT ATCCCTATAT AACCTACCCA TCCACCTTTC GCTCCTTGAA CTTGCATCTA
  781 AACTCGACCT CTACATTTTT TATGTTTATC TCTAGTATTA CTCTTTAGAC AAAAAAAATT
  841 GTAGTAAGAA CTATTCATAG AGTGAATCGA AAACAATACG AAAATGTAAA CATTTTCCTA
  901 TACGTAGTAT ATAGAGACAA AATAGAAGAA ACCGTTCATA ATTTTCTGAC CAATGAAGAA
  961 TCATCAACGC TATCACTTTC TGTTCACAAA GTATGCGCAA TCCACATCGG TATAGAATAT
 1021 AATCGGGGAT GCCCTTTATC TTGAAAAAAA TGCCACCGCC AGCCTTCGCT AGGTAATTCA
 1081 GTTAAACGCG GGAAGGTTGG AAGTCAGGCT TTTTTATGAA AGAGAAGATT AGGAACGTCA
1141 AAAAGTAAGG CCATTC
//
Primer: HIS3pro_F RDB13121_A5Bgc.seq
>01948_13121_A5Bg_HIS3pro_F_C10_07.ab1
    1 TGGCAATATA CATTATATAA AGTAATGTGA TTTCTTCGAA GAATATACTA AAAAATGAGC
   61 AGGCAAGATA AACGAAGGCA AAGATGACAG AGCAGAAAGC CCTAGTAAAG CGTATTACAA
  121 ATGAAACCAA GATTCAGATT GCGATCTCTT TAAAGGGTGG TCCCCTAGCG ATAGAGCACT
  181 CGATCTTCCC AGAAAAAGAG GCAGAAGCAG TAGCAGAACA GGCCACACAA TCGCAAGTGA
  241 TTAACGTCCA CACAGGTATA GGGTTTCTGG ACCATATGAT ACATGCTCTG GCCAAGCATT
  301 CCGGCTGGTC GCTAATCGTT GAGTGCATTG GTGACTTACA CATAGACGAC CATCACACCA
  361 CTGAAGACTG CGGGATTGCT CTCGGTCAAG CATTTAAAGA GGCCCTAGGG GCCGTGCGTG
  421 GAGTAAAAAG GTTTGGATCA GGATTTGCGC CTTTGGATGA GGCACTTTCC AGAGCGGTGG
  481 TAGATCTTTC GAACAGGCCG TACGCAGTTG TCGAACTTGG TTTGCAGAGG GAGAAAGTAG
  541 GAGATCTCTC TTGCGAGATG ATCCCGCATT TTCTTGAAAG TTTTGCAGAG GCAAGCAGAA
  601 TTACCCTCCA CGTTGATTGT CTGCGAGGCA AGAATGATCA TCACCGTAGT GAGAGTGCGT
  661 TCAAGGCTCT TGCGGTTGCC ATAAGAGAAG CCACCTCGCC CAATGGAACC AACGATGTTC
  721 CCTCCACCAA AGGTGTTCTT ATGTAGTGAC ACCGATTATT TAAAGCGGCA AGCTTACATT
  781 TTATGTTAGC TGGTGGACTG ACGCCAGAAA ATGTTGGTGA TGCGCTTAGA TTAAATGGCG
  841 TTATTGGTGT TGATGTAAGC GGAGGTGTGG AGACAAATGG TGTAAAAGAC TCTAACAAAA
  901 TAGCAAATTT CGTCAAAAAT GCTAAGAAAT AGGTTATTAC TGAGTAGTAT TTATTTAAGT
  961 ATTTGTTTGT GCACTTGCCC GGTAGAGGTG TGGTCAATAA GAGCGACCTC ATGCTATACC
 1021 CTGAGAAAGC AACCCTGACC CTACAGGAAA GAGTTTACTC AAGAATAAGA TTTTCGTTTT
 1081 AAAACCTAAG AGTTCACTTT AAAATTTGTA TAACACTTAC TTTTTTTGAT GACTTAGTCA
1141 TATAGAGATT CATTTAGAAG TTA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

2018.12.11

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