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pAMH10

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01947
Resource name pAMH10
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), centromere-type ori, HIS3, ADH1 promoter, Ampicillin
Vector backbone pAMH10 (Plasmid)
Size of vector backbone 4,495 bp
Selectable markers Amp^r (E. coli), HIS3 (S. cerevisiae)
Depositor Iha, Hidekatsu |

Distribution information

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RDB01947 pAMH10 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB01947z.seq from Depositor
Electronic file Map RDB01947.gif from Depositor
Electronic file Map RDB01947z.gif from Depositor

Featured content

Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13042_A4Lj.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev RDB13042_A4Lja.seq
>01947_13042_A4Lj_ADHrev_G08_20.ab1
    1 GTGCTATCAG ATAAGATTTT CGTTTTAAAC CTAAGAGTCA CTTTAAAATT TGTATACACT
   61 TATTTTTTTT ATAACTTATT TAATAATAAA AATCATAAAT CATAAGAAAT TCGCCCGGAA
  121 TTAGCTTGGC TGCAGGTCGA CTCACAGATC CTCTTCGGAA ATCAGTTTCT GTTCAGAATT
  181 CGAGCTCGGT ACCCGGGGAT CCTCTAGAGT CAGCTTGGAG TTGATTGTAT GCTTGGTATA
  241 GCTTGAAATA TTGTGCAGAA AAAGAAACAA GGAAGAAAGG GAACGAGAAC AATGACGAGG
  301 AAACAAAAGA TTAATAATTG CAGGTCTATT TATACTTGAT AGCAAGACAG CAAACTTTTT
  361 TTTATTTCAA ATTCAAGTAA CTGGAAGGAA GGCCGTATAC CGTTGCTCAT TAGAGAGTAG
  421 TGTGCGTGAA TGAAGGAAGG AAAAAGTTTC GTGTGCTTCG AGATACCCCT CATCAGCTCT
  481 GGAACAACGA CATCTGTTGG TGCTGTCTTT GTCGTTAATT TTTTCCTTTA GTGTCTTCCA
  541 TCATTTTTTT GTCATTGCGG ATATGGTGAG ACAACAACGG GGGAGAGAGA AAAGAAAAAA
  601 AAAGAAAAGA AGTTGCATGC AAGCTCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA
  661 AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA
  721 TAATGGTTTC TTAGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT
  781 TTAATATAAA TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT
  841 TTTTGTTTTC CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT
  901 CTTGCTCTTC CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA
  961 CCACACACGA AAATCCTGTG ATTTTACATT TTACTTTATC GTTAATCGAA ATGTATATCT
 1021 ATTTAATCTG CTTTTCTGTC TAATTAAATA TATATGTAAG TACGCTTTTT TGTGAAATTT
 1081 TTAAACCTTT GATTATGTTT TCTTCATTCG GTAACTCGTC CTACCGTCTT TAATTTAACG
1141 TTTTCTCATA
//
Primer: Amp_R RDB13042_A4Ljb.seq
>01947_13042_A4Lj_Amp_R_H08_23.ab1
    1 TCGTTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGACGGATC GCTTGCCTGT AACTTACACG CGCCTCGTAT CTTTTAATGA TGGAATAATT
  181 TGGGAATTTA CTCTGTGTTT ATTTATTTTT ATGTTTTGTA TTTGGATTTT AGAAAGTAAA
  241 TAAAGAAGGT AGAAGAGTTA CGGAATGAAG AAAAAAAAAT AAACAAAGGT TTAAAAAATT
  301 TCAACAAAAA GCGTACTTTA CATATATATT TATTAGACAA GAAAAGCAGA TTAAATAGAT
  361 ATACATTCGA TTAACGATAA GTAAAATGTA AAATCACAGG ATTTTCGTGT GTGGTCTTCT
  421 ACACAGACAA GATGAAACAA TTCGGCATTA ATACCTGAGA GCAGGAAGAG CAAGATAAAA
  481 GGTAGTATTT GTTGGCGATC CCCCTAGAGT CTTTTACATC TTCGGAAAAC AAAAACTATT
  541 TTTTCTTTAA TTTCTTTTTT TACTTTCTAT TTTTAATTTA TATATTTATA TTAAAAAATT
  601 TAAATTATAA TTATTTTTAT AGCACGTGAT GAAAAGGACC CTAAGAAACC ATTATTATCA
  661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
  721 ATGACGGTGA AAACCTCTGA CACATGCAGA GCTTGCATGC AACTTCTTTT CTTTTTTTTT
  781 CTTTTCTCTC TCCCCCGTTG TTGTCTCACC ATATCCGCAA TGACAAAAAA ATGATGGAAG
  841 ACACTAAAGG AAAAAATTAA CGACAAAGAC AGCACCAACA GATGTCGTTG TTCCAGAGCT
  901 GATGAGGGGT ATCTCGAAGC ACACGAAACT TTTTCCTTCC TTCATTCACG CACACTACTC
  961 TCTAATGAGC AACGGTATAC GGCCTTCCTT CCAGTTACTT GAATTTGAAA TAAAAAAAAA
 1021 GTTTGCTGTC TTGCTATCAA GTATAAAATA GACCTGCAGT ATAATCTTTT GTTTCCTTCG
 1081 TCATGTCTCG ATCCCTTTTC TTCTTGCTCC TTTTTCCTGG CACAATTATT TCAAGGCTTA
1141 TTACCAAGGC AATTAACCAA ATTCCAA
//
Primer: HIS3pro_F RDB13042_A4Ljc.seq
>01947_13042_A4Lj_HIS3pro_F_A09_03.ab1
    1 TAGCAATTAT ACATTATATA AAGTAATGTG ATTTCTTCGA AGAATATACT AAAAAATGAG
   61 CAGGCAAGAT AAACGAAGGC AAAGATGACA GAGCAGAAAG CCCTAGTAAA GCGTATTACA
  121 AATGAAACCA AGATTCAGAT TGCGATCTCT TTAAAGGGTG GTCCCCTAGC GATAGAGCAC
  181 TCGATCTTCC CAGAAAAAGA GGCAGAAGCA GTAGCAGAAC AGGCCACACA ATCGCAAGTG
  241 ATTAACGTCC ACACAGGTAT AGGGTTTCTG GACCATATGA TACATGCTCT GGCCAAGCAT
  301 TCCGGCTGGT CGCTAATCGT TGAGTGCATT GGTGACTTAC ACATAGACGA CCATCACACC
  361 ACTGAAGACT GCGGGATTGC TCTCGGTCAA GCATTTAAAG AGGCCCTAGG GGCCGTGCGT
  421 GGAGTAAAAA GGTTTGGATC AGGATTTGCG CCTTTGGATG AGGCACTTTC CAGAGCGGTG
  481 GTAGATCTTT CGAACAGGCC GTACGCAGTT GTCGAACTTG GTTTGCAGAG GGAGAAAGTA
  541 GGAGATCTCT CTTGCGAGAT GATCCCGCAT TTTCTTGAAA GTTTTGCAGA GGCAAGCAGA
  601 ATTACCCTCC ACGTTGATTG TCTGCGAGGC AAGAATGATC ATCACCGTAG TGAGAGTGCG
  661 TTCAAGGCTC TTGCGGTTGC CATAAGAGAA GCCACCTCGC CCAATGGAAC CAACGATGTT
  721 CCCTCCACCA AAGGTGTTCT TATGTAGTGA CACCGATTAT TTAAAGCGGC AAGCTTACAT
  781 TTTATGTTAG CTGGTGGACT GACGCCAGAA AATGTTGGTG ATGCGCTTAG ATTAAATGGC
  841 GTTATTGGTG TTGATGTAAG CGGAGGTGTG GAGACAAATG GTGTAAAAGA CTCTAACAAA
  901 ATAGCAAATT TCGTCAAAAA TGCTAAGAAA TAGGTTATTA CTGAGTAGTA TTTATTTAAG
  961 TATTGTTTGT GCACTTGCCG GTAGAGGTGT GGTCAATAAG AGCGACCTCA TGCTATACCT
 1021 GAGAAGCAAC CCTGACCTAC AGGAAAGAGT TACTCAAGAA TAAGAATTTT CGTTTTAAAC
 1081 CTAGGAGTCA CTTTAAAATT TGTATACACT TATTTTTTTT TTTATACCTA TTATATAAAA
1141 ATTCATTAAA TCCATAAGAA ATTCCGCCCG GGAATATAAG CCTGGGGCCT GC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

2018.12.11

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