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Lambda GEX5 (#RDB01911)

Lambda phage cloning vector producing GST fusion protein.

Clone info. Lambda phage cloning vector producing GST fusion protein. Derivative of lambda gt11. Use E.coli BB4 as host strain.
Vector backbone Lambda GEX5 (Phage)
Growth conditions 37 C,
Depositor|Developer Fukunaga, Rikiro |
 
Sequence (full) RDB01911hts02.seq checkAssembled from experimentally sequenced data.
Additional map
Publication Fukunaga, R., EMBO J., 16, 1921-1933 (1997). PMID 9155018. [link to RRC of NBRP]
Test sheet RDB01911C0p1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (EMBO J., 16, 1921-1933 (1997)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (EMBO J., 16, 1921-1933 (1997))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01911 Lambda GEX5 DNA solution or Phage particle JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The Lambda GEX5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01911).

Reference section:

Fukunaga, R., Hunter, T., MNK1, a new MAP kinase-activated protein kinase, isolated by a novel expression screening method for identifying protein kinase substrates. EMBO J., 16, 1921-1933 (1997). PMID 9155018. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Fukunaga, R., MNK1, a new MAP kinase-activated protein kinase, isolated by a novel expression screening method for identifying protein kinase substrates. EMBO J., 16, 1921-1933 (1997). PMID 9155018. [link to RRC of NBRP]