Resource data sheet
Prev.

pCAUT

E. coli UPRTase + HSV-TK, genomic DNA

Catalog number RDB01903
Resource name pCAUT
Alternative name E. coli UPRTase + HSV-TK genomic DNA
Clone info. Expression vector of E. coli UPRTase and HSV-TK fusion protein.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Amp^r (E. coli)
Gene/insert name E. coli uracil phosphoribosyl transferase (UPRTase) Genomic DNA HSV thymidine kinase genomic DNA
Depositor Hamada, Hirofumi |

Distribution information

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Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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RDB01903 pCAUT DNA solution Please contact us.

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References and tips

Electronic file

Electronic file Map RDB01903.gif from Depositor

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13439_A5Fi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB13439_A5Fia.seq
>01903_13439_A5Fi_pAxCA_F1_F05_17.ab1
    1 CTCGCCGAAG GAACCGAAAT TCTCATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG
   61 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCCAC CATGAAGATC
  121 GTGGAAGTCA AACACCCACT CGTCAAACAC AAGCTGGGAC TGATGCGTGA GCAAGATATC
  181 AGCACCAAGC GCTTTCGCGA ACTCGCTTCC GAAGTGGGTA GCCTGCTGAC TTACGAAGCG
  241 ACCGCCGACC TCGAAACGGA AAAAGTAACT ATCGAAGGCT GGAACGGCCC GGTAGAAATC
  301 GACCAGATCA AAGGTAAGAA AATTACCGTT GTGCCAATTC TGCGTGCGGG TCTTGGTATG
  361 ATGGACGGTG TGCTGGAAAA CGTTCCGAGC GCGCGCATCA GCGTTGTCGG TATGTACCGT
  421 AATGAAGAAA CGCTGGAGCC GGTACCGTAC TTCCAGAAAC TGGTTTCTAA CATCGATGAG
  481 CGTATGGCGC TGATCGTTGA CCCAATGCTG GCAACCGGTG GTTCCGTTAT CGCGACCATC
  541 GACCTGCTGA AAAAAGCGGG CTGCAGCAGC ATCAAAGTTC TGGTGCTGGT AGCTGCGCCA
  601 GAAGGTATCG CTGCGCTGGA AAAAGCGCAC CCGGACGTCG AACTGTATAC CGCATCGATT
  661 GATCAGGGAC TGAACGAGCA CGGATACATT ATTCCGGGCC TCGGCGATGC CGGTGACAAA
  721 ATCTTTGGTA CGAAAGGAGG ATCCGGCGGG GGCGGTGGAG GAGGGGGTAT GGCTTCGTAC
  781 CCCTGCCATC AACACGCGTC TGCGTTCGAC CAGGCTGCGC GTTCTCGCGG CCATAGCAAC
  841 CGACGTACGG CGTTGCGCCC TCGCCGGCAG CAAGAAGCCA CGGAAGTCCG CCTGGAGCAG
  901 AAAATGCCCA CGCTACTGCG GGTTTATATA GACGGTCCTC ACGGGATGGG GAAAACCACC
  961 ACCACGCAAC TGCTGGTGGC CCTGGGTTCG CGCGACGATA TCGTCTACGT ACCCGAGCCG
 1021 ATGACTTACT GGCAGGTGCT GGGGGCTTCC GAGACAATCG CGAACATCTA CACCACACAA
 1081 CACCGCCTCG ACACGTGAGA TATCGGCCGG GGGACGCGCC GTTGTATGAC AGCGCCCAGA
1141 ATACATGGGC ATGCCTTATT GCCCGTGAAC CGAACGCCCG ATTG
//
Primer: HSV TK pA_R RDB13439_A5Fib.seq
>01903_13439_A5Fi_HSVTKpA_R_H09_24.ab1
    1 AGGGGGATCT TCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCA TCTCCCGGGC
   61 AAACGTGCGC GCCAGGTCGC AGATCGTCGG TATGGAGCCG GGGGTGGTGA CGTGGGTCTG
  121 GACCATCCCG GAGGTAAGTT GCAGCAGGGC GTCCCGGCAG CCGGCGGGCG ATTGGTCGTA
  181 ATCCAGGATA AAGACGTGCA TGGGACGGAG GCGTTTGGCC AAGACGTCCA AGGCCCAGGC
  241 AAACACGTTG TACAGGTCGC CGTTGGGGGC CAGCAACTCG GGGGCCCGAA ACAGGGTAAA
  301 TAACGTGTCC CCGATATGGG GTCGTGGGCC CGCGTTGCTC TGGGGCTCGG CACCCTGGGG
  361 CGGCACGGCC GTCCCCGAAA GCTGTCCCCA ATCCTCCCGC CACGACCCGC CGCCCTGCAG
  421 ATACCGCACC GTATTGGCAA GCAGCCCGTA AACGCGGCGA ATCGCGGCCA GCATAGCCAG
  481 GTCAAGCCGC TCGCCGGGGC GCTGGCGTTT GGCCAGGCGG TCGATGTGTC TGTCCTCCGG
  541 AAGGGCCCCC AACACGATGT TTGTGCCGGG CAAGGTCGGC GGGATGAGGG CCACGAACGC
  601 CAGCACGGCC TGGGGGGTCA TGCTGCCCAT AAGGTATCGC GCGGCCGGGT AGCACAGGAG
  661 GGCGGCGATG GGATGGCGGT CGAAGATGAG GGTGAGGGCC GGGGGCGGGG CATGTGAGCT
  721 CCCAGCCTCC CCCCCGATAT GAGGAGCCAG AACGGCGTCG GTCACGGCAT AAGGCATGCC
  781 CATTGTTATC TGGGCGCTTG TCATTACCAC CGCCGCGTCC CCGGCCGATA TCTCACCCTG
  841 GTCGAGGCGG TGTTGTGTGG TGTAGATGTT CGCGATTGTC TCGGAAGCCC CCAGCACCTG
  901 CCAGTAAGTC ATCGGCTCGG GTACGTAGAC GATATCGTCG CGCGAACCCA GGGCCACCAG
  961 CAGTTGCGTG GTGGTGGTTT TCCCCATCCC GTGAGGACCG TCTATATAAA CCCGCAGTAG
 1021 CGTGGGCATT TTCTGCTCCA GGCGGGACTT CCGTGGCTTC TTGCTGCCGG CGAGGGCGCA
 1081 ACGCCCGTAC GTCGCTTGCT ATGGCCGCGA GAACGCGCAG CTTATTCGGA CGGCAGACGC
1141 CGTGTTGATG GCCAAGGGGA TAACGAAGCC ATATACCCCC CCATCGT
//
Primer: Reverse2 RDB13439_A5Fic.seq
>01903_13439_A5Fi_Reverse2_G09_21.ab1
    1 AATACCTGAT TACGCCAGCT TCTGATGGAA TTAGAACTTG GCAAAACAAT ACTGAGAATG
   61 AAGTGTATGT GGAACAGAGG CTGCTGATCT CGTTCTTCAG GCTATGAAAC TGACACATTT
  121 GGAAACCACA GTACTTAGAA CCACAAAGTG GGAATCAAGA GAAAAACAAT GATCCCACGA
  181 GAGATCTATA GATCTATAGA TCATGAGTGG GAGGAATGAG CTGGCCCTTA ATTTGGTTTT
  241 GCTTGTTTAA ATTATGATAT CCAACTATGA AACATTATCA TAAAGCAATA GTAAAGAGCC
  301 TTCAGTAAAG AGCAGGCATT TATCTAATCC CACCCCACCC CCACCCCCGT AGCTCCAATC
  361 CTTCCATTCA AAATGTAGGT ACTCTGTTCT CACCCTTCTT AACAAAGTAT GACAGGAAAA
  421 ACTTCCATTT TAGTGGACAT CTTTATTGTT TAATAGATCA TCAATTTCTG CAGGTCCTGG
  481 CCGGGGTCCC GCGGAAACTC GGCCGTGGTG ACCAATACAA AACAAAAGCG CTCCTCGTAC
  541 CAGCGAAGAA GGGGCAGAGA TGCCGTAGTC AGGTTTAGTT CGTCCGGCGG CGCCAGAAAT
  601 CCGCGCGGTG GTTTTTGGGG GTCGGGGGTG TTTGGCAGCC ACAGACGCCC GGTGTTCGTG
  661 TCGCGCCAGT ACATGCGGTC CATGCCCAGG CCATCCAAAA ACCATGGGTC TGTCTGCTCA
  721 GTCCAGTCGT GGACCTGACC CCACGCAACG CCCAAAATAA TAACCCCCAC GAACCATAAA
  781 CCATTCCCCA TGGGGGACCC CGTCCCTAAC CCACGGGGCC CGTGGCTATG GCAGGGCTTG
  841 CCGCCCCGAC GTTGGCTGCG AGCCCTGGGG CCTTCACCCG AACTTGGGGG GTGGGGGTGG
  901 GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA
  961 GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC ACCCGTGCGT
 1021 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TTCCGGTATT GTCTCTTTCC
 1081 GTGTTTCAGT TTAGCCTCCC CCATCTCCGG GCAACGTGGC GCGCAGGTTC GCAGATCGTT
1141 CGGGTGGAAG CCCGGGGGTG GTGACGGTGG GGTTCTTGGA CCCCAATC
//
Primer: SV40pA3'Element Reverse RDB13439_A5Fid.seq
>01903_13439_A5Fi_SV40pA3'ElementReverse_F12_18.ab1
    1 GCTCAGAGGG CGGAGCCTAT GGAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT
   61 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC
  121 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG
  181 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT
  241 GGCCGATTCA TTAATGCAGC GGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA
  301 ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT
  361 TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAGCT TTTTGCAAAA GCCTAGGCCT
  421 CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT
  481 GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT
  541 AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCGGA
  601 TCCGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG
  661 TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT
  721 TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG
  781 CCAAGCTTCT GATGGAATTA GAACTTGGCA AAACAATACT GAGAATGAAG TGTATGTGGA
  841 ACAGAGGCTG CTGATCTCGT TCTTCAGGCT ATGAAACTGA CACATTTGGA AACCACAGTA
  901 CTTAGAACCA CAAAGTGGGA ATCAAGAGAA AAACAATGAT CCCACGAGAG ATCTATAGAT
  961 CTATAGATCA TGAGTGGGAG GAATGAGCTG GCCCTTAATT TGGGTTTTGC TTGTTTAAAT
 1021 TATGATATCC ACTATGAAAC ATTATCATAA GCATAGTAAG AGCTTCAGTA AGAGCAGCAT
 1081 TTATCTATTC CACCCACCCC CACCCCCGTA GCTCAATCCT TCCAATTCAA TGTAAGGTAC
1141 TCTGGTTCTC ACCTTCTTAA CAAAGTATTG AAACAAGGGA
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

GNP_filter3_RDBDEP_html_181012.pl