Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCAUT

E. coli UPRTase + HSV-TK, genomic DNA

Remarks, protocol and/or map (gif)	RDB01903.gif

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

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Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]

提供案内 (日本国内) [open/close]

提供条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13439_A5Fi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB13439_A5Fia.seq
>01903_13439_A5Fi_pAxCA_F1_F05_17.ab1     1 CTCGCCGAAG GAACCGAAAT TCTCATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG    61 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCCAC CATGAAGATC   121 GTGGAAGTCA AACACCCACT CGTCAAACAC AAGCTGGGAC TGATGCGTGA GCAAGATATC   181 AGCACCAAGC GCTTTCGCGA ACTCGCTTCC GAAGTGGGTA GCCTGCTGAC TTACGAAGCG   241 ACCGCCGACC TCGAAACGGA AAAAGTAACT ATCGAAGGCT GGAACGGCCC GGTAGAAATC   301 GACCAGATCA AAGGTAAGAA AATTACCGTT GTGCCAATTC TGCGTGCGGG TCTTGGTATG   361 ATGGACGGTG TGCTGGAAAA CGTTCCGAGC GCGCGCATCA GCGTTGTCGG TATGTACCGT   421 AATGAAGAAA CGCTGGAGCC GGTACCGTAC TTCCAGAAAC TGGTTTCTAA CATCGATGAG   481 CGTATGGCGC TGATCGTTGA CCCAATGCTG GCAACCGGTG GTTCCGTTAT CGCGACCATC   541 GACCTGCTGA AAAAAGCGGG CTGCAGCAGC ATCAAAGTTC TGGTGCTGGT AGCTGCGCCA   601 GAAGGTATCG CTGCGCTGGA AAAAGCGCAC CCGGACGTCG AACTGTATAC CGCATCGATT   661 GATCAGGGAC TGAACGAGCA CGGATACATT ATTCCGGGCC TCGGCGATGC CGGTGACAAA   721 ATCTTTGGTA CGAAAGGAGG ATCCGGCGGG GGCGGTGGAG GAGGGGGTAT GGCTTCGTAC   781 CCCTGCCATC AACACGCGTC TGCGTTCGAC CAGGCTGCGC GTTCTCGCGG CCATAGCAAC   841 CGACGTACGG CGTTGCGCCC TCGCCGGCAG CAAGAAGCCA CGGAAGTCCG CCTGGAGCAG   901 AAAATGCCCA CGCTACTGCG GGTTTATATA GACGGTCCTC ACGGGATGGG GAAAACCACC   961 ACCACGCAAC TGCTGGTGGC CCTGGGTTCG CGCGACGATA TCGTCTACGT ACCCGAGCCG  1021 ATGACTTACT GGCAGGTGCT GGGGGCTTCC GAGACAATCG CGAACATCTA CACCACACAA  1081 CACCGCCTCG ACACGTGAGA TATCGGCCGG GGGACGCGCC GTTGTATGAC AGCGCCCAGA 1141 ATACATGGGC ATGCCTTATT GCCCGTGAAC CGAACGCCCG ATTG //
Primer: HSV TK pA_R Sequence file: RDB13439_A5Fib.seq
>01903_13439_A5Fi_HSVTKpA_R_H09_24.ab1     1 AGGGGGATCT TCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCA TCTCCCGGGC    61 AAACGTGCGC GCCAGGTCGC AGATCGTCGG TATGGAGCCG GGGGTGGTGA CGTGGGTCTG   121 GACCATCCCG GAGGTAAGTT GCAGCAGGGC GTCCCGGCAG CCGGCGGGCG ATTGGTCGTA   181 ATCCAGGATA AAGACGTGCA TGGGACGGAG GCGTTTGGCC AAGACGTCCA AGGCCCAGGC   241 AAACACGTTG TACAGGTCGC CGTTGGGGGC CAGCAACTCG GGGGCCCGAA ACAGGGTAAA   301 TAACGTGTCC CCGATATGGG GTCGTGGGCC CGCGTTGCTC TGGGGCTCGG CACCCTGGGG   361 CGGCACGGCC GTCCCCGAAA GCTGTCCCCA ATCCTCCCGC CACGACCCGC CGCCCTGCAG   421 ATACCGCACC GTATTGGCAA GCAGCCCGTA AACGCGGCGA ATCGCGGCCA GCATAGCCAG   481 GTCAAGCCGC TCGCCGGGGC GCTGGCGTTT GGCCAGGCGG TCGATGTGTC TGTCCTCCGG   541 AAGGGCCCCC AACACGATGT TTGTGCCGGG CAAGGTCGGC GGGATGAGGG CCACGAACGC   601 CAGCACGGCC TGGGGGGTCA TGCTGCCCAT AAGGTATCGC GCGGCCGGGT AGCACAGGAG   661 GGCGGCGATG GGATGGCGGT CGAAGATGAG GGTGAGGGCC GGGGGCGGGG CATGTGAGCT   721 CCCAGCCTCC CCCCCGATAT GAGGAGCCAG AACGGCGTCG GTCACGGCAT AAGGCATGCC   781 CATTGTTATC TGGGCGCTTG TCATTACCAC CGCCGCGTCC CCGGCCGATA TCTCACCCTG   841 GTCGAGGCGG TGTTGTGTGG TGTAGATGTT CGCGATTGTC TCGGAAGCCC CCAGCACCTG   901 CCAGTAAGTC ATCGGCTCGG GTACGTAGAC GATATCGTCG CGCGAACCCA GGGCCACCAG   961 CAGTTGCGTG GTGGTGGTTT TCCCCATCCC GTGAGGACCG TCTATATAAA CCCGCAGTAG  1021 CGTGGGCATT TTCTGCTCCA GGCGGGACTT CCGTGGCTTC TTGCTGCCGG CGAGGGCGCA  1081 ACGCCCGTAC GTCGCTTGCT ATGGCCGCGA GAACGCGCAG CTTATTCGGA CGGCAGACGC 1141 CGTGTTGATG GCCAAGGGGA TAACGAAGCC ATATACCCCC CCATCGT //
Primer: Reverse2 Sequence file: RDB13439_A5Fic.seq
>01903_13439_A5Fi_Reverse2_G09_21.ab1     1 AATACCTGAT TACGCCAGCT TCTGATGGAA TTAGAACTTG GCAAAACAAT ACTGAGAATG    61 AAGTGTATGT GGAACAGAGG CTGCTGATCT CGTTCTTCAG GCTATGAAAC TGACACATTT   121 GGAAACCACA GTACTTAGAA CCACAAAGTG GGAATCAAGA GAAAAACAAT GATCCCACGA   181 GAGATCTATA GATCTATAGA TCATGAGTGG GAGGAATGAG CTGGCCCTTA ATTTGGTTTT   241 GCTTGTTTAA ATTATGATAT CCAACTATGA AACATTATCA TAAAGCAATA GTAAAGAGCC   301 TTCAGTAAAG AGCAGGCATT TATCTAATCC CACCCCACCC CCACCCCCGT AGCTCCAATC   361 CTTCCATTCA AAATGTAGGT ACTCTGTTCT CACCCTTCTT AACAAAGTAT GACAGGAAAA   421 ACTTCCATTT TAGTGGACAT CTTTATTGTT TAATAGATCA TCAATTTCTG CAGGTCCTGG   481 CCGGGGTCCC GCGGAAACTC GGCCGTGGTG ACCAATACAA AACAAAAGCG CTCCTCGTAC   541 CAGCGAAGAA GGGGCAGAGA TGCCGTAGTC AGGTTTAGTT CGTCCGGCGG CGCCAGAAAT   601 CCGCGCGGTG GTTTTTGGGG GTCGGGGGTG TTTGGCAGCC ACAGACGCCC GGTGTTCGTG   661 TCGCGCCAGT ACATGCGGTC CATGCCCAGG CCATCCAAAA ACCATGGGTC TGTCTGCTCA   721 GTCCAGTCGT GGACCTGACC CCACGCAACG CCCAAAATAA TAACCCCCAC GAACCATAAA   781 CCATTCCCCA TGGGGGACCC CGTCCCTAAC CCACGGGGCC CGTGGCTATG GCAGGGCTTG   841 CCGCCCCGAC GTTGGCTGCG AGCCCTGGGG CCTTCACCCG AACTTGGGGG GTGGGGGTGG   901 GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA   961 GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC ACCCGTGCGT  1021 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TTCCGGTATT GTCTCTTTCC  1081 GTGTTTCAGT TTAGCCTCCC CCATCTCCGG GCAACGTGGC GCGCAGGTTC GCAGATCGTT 1141 CGGGTGGAAG CCCGGGGGTG GTGACGGTGG GGTTCTTGGA CCCCAATC //
Primer: SV40pA3'Element Reverse Sequence file: RDB13439_A5Fid.seq
>01903_13439_A5Fi_SV40pA3'ElementReverse_F12_18.ab1     1 GCTCAGAGGG CGGAGCCTAT GGAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT    61 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC   121 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG   181 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT   241 GGCCGATTCA TTAATGCAGC GGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA   301 ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT   361 TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAGCT TTTTGCAAAA GCCTAGGCCT   421 CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT   481 GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT   541 AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCGGA   601 TCCGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG   661 TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT   721 TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG   781 CCAAGCTTCT GATGGAATTA GAACTTGGCA AAACAATACT GAGAATGAAG TGTATGTGGA   841 ACAGAGGCTG CTGATCTCGT TCTTCAGGCT ATGAAACTGA CACATTTGGA AACCACAGTA   901 CTTAGAACCA CAAAGTGGGA ATCAAGAGAA AAACAATGAT CCCACGAGAG ATCTATAGAT   961 CTATAGATCA TGAGTGGGAG GAATGAGCTG GCCCTTAATT TGGGTTTTGC TTGTTTAAAT  1021 TATGATATCC ACTATGAAAC ATTATCATAA GCATAGTAAG AGCTTCAGTA AGAGCAGCAT  1081 TTATCTATTC CACCCACCCC CACCCCCGTA GCTCAATCCT TCCAATTCAA TGTAAGGTAC 1141 TCTGGTTCTC ACCTTCTTAA CAAAGTATTG AAACAAGGGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.18

GNP_filter3_RDBDEP_html_230702.pl