Resource data sheet
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pCAhCTLA4Ig

Expression clone of human CTLA4 and IgG1

Catalog number RDB01886
Resource name pCAhCTLA4Ig
Alternative name pCAccCTLA4Ig
Clone info. Expression clone of uman CTLA4 and IgG1.
Vector backbone pCAcc (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Amp^r
Gene/insert name Human CTLA4 cDNA Human cDNA
Depositor Hamada, Hirofumi |
 
Other clones in our bank human CTLA4 (NCBI Gene 1493) | human IGHG1 (NCBI Gene 3500) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Science, 245, 631-635 (1989).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01886 pCAhCTLA4Ig DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01886.gif from Depositor

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References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12738_A4Bs.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCAF1 RDB12738_A4Bsa.seq
>01886_12738_A4Bs_pAxCAF1_A11_02.ab1
    1 TCGGCGGGGC GGAGTACGAT CTTCTCATGT TATGCCTTCT TCTTTTTCCT ACAGCTCCTG
   61 GGCAACGTGC TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTCGA TAAGCTTCAC
  121 CATGGGTGTA CTGCTCACAC AGAGGACGCT GCTCAGTCTG GTCCTTGCAC TCCTGTTTCC
  181 AAGCATGGCG AGCATGGCAA TGCACGTGGC CCAGCCTGCT GTGGTACTGG CCAGCAGCCG
  241 AGGCATCGCC AGCTTTGTGT GTGAGTATGC ATCTCCAGGC AAAGCCACTG AGGTCCGGGT
  301 GACAGTGCTT CGGCAGGCTG ACAGCCAGGT GACTGAAGTC TGTGCGGCAA CCTACATGAT
  361 GGGGAATGAG TTGACCTTCC TAGATGATTC CATCTGCACG GGCACCTCCA GTGGAAATCA
  421 AGTGAACCTC ACTATCCAAG GACTGAGGGC CATGGACACG GGACTCTACA TCTGCAAGGT
  481 GGAGCTCATG TACCCACCGC CATACTACCT GGGCATAGGC AACGGAACCC AGATTTATGT
  541 AATTGATCCA GAACCGTGCC CAGATTCTGA TCAGGAGCCC AAATCTTCTG ACAAAACTCA
  601 CACATCCCCA CCGTCCCCAG CACCTGAACT CCTGGGGGGA CCGTCAGTCT TCCTCTTCCC
  661 CCCAAAACCC AAGGACACCC TCATGATCTC CCGGACCCCT GAGGTCACAT GCGTGGTGGT
  721 GGACGTGAGC CACGAAGACC CTGAGGTCAA GTTCAACTGG TACGTGGACG GCGTGGAGGT
  781 GCATAATGCC AAGACAAAGC CGCGGGAGGA GCAGTACAAC AGCACGTACC GTGTGGTCAG
  841 CGTCCTCACC GTCCTGCACC AGGACTGGCT GAATGGCAAG GAGTACAAGT GCAAGGTCTC
  901 CAACAAAGCC CTCCCAGCCC CCATCGAGAA AACCATCTCC AAAGCCAAAG GGCAGCCCCG
  961 AGAACCACAG GTGTACACCC TGCCCCCATC CCGGGATGAG CTGACCAAGA AACCAGGGTC
 1021 AGCCCTGACC CTGCCCTGGT CAAGGCTTCT ATCCCAGCGA CATCGCCCGT GGAGTGGGAG
 1081 AGCAATGGGG CAGCCCGGGA GAACACTACT AGGACACGGC ATCCGATGCC TGACTCCGAC
1141 GGCCTTCCTT TCTTTCCCTC C
//
Primer: pAxCA_R1 RDB12738_A4Bsb.seq
>01886_12738_A4Bs_pAxCAR1_B11_05.ab1
    1 TTTGGGGAAA AGGGCATTGG CCACACCAGC CACCACCTTC TGATAGGCAG CCTGCACCTG
   61 AGGAGTGAAT TCTAGATTAT TTACCCGGAG ACAGGGAGAG GCTCTTCTGC GTGTAGTGGT
  121 TGTGCAGAGC CTCATGCATC ACGGAGCATG AGAAGACGTT CCCCTGCTGC CACCTGCTCT
  181 TGTCCACGGT GAGCTTGCTG TAGAGGAAGA AGGAGCCGTC GGAGTCCAGC ACGGGAGGCG
  241 TGGTCTTGTA GTTGTTCTCC GGCTGCCCAT TGCTCTCCCA CTCCACGGCG ATGTCGCTGG
  301 GATAGAAGCC TTTGACCAGG CAGGTCAGGC TGACCTGGTT CTTGGTCAGC TCATCCCGGG
  361 ATGGGGGCAG GGTGTACACC TGTGGTTCTC GGGGCTGCCC TTTGGCTTTG GAGATGGTTT
  421 TCTCGATGGG GGCTGGGAGG GCTTTGTTGG AGACCTTGCA CTTGTACTCC TTGCCATTCA
  481 GCCAGTCCTG GTGCAGGACG GTGAGGACGC TGACCACACG GTACGTGCTG TTGTACTGCT
  541 CCTCCCGCGG CTTTGTCTTG GCATTATGCA CCTCCACGCC GTCCACGTAC CAGTTGAACT
  601 TGACCTCAGG GTCTTCGTGG CTCACGTCCA CCACCACGCA TGTGACCTCA GGGGTCCGGG
  661 AGATCATGAG GGTGTCCTTG GGTTTTGGGG GGAAGAGGAA GACTGACGGT CCCCCCAGGA
  721 GTTCAGGTGC TGGGGACGGT GGGGATGTGT GAGTTTTGTC AGAAGATTTG GGCTCCTGAT
  781 CAGAATCTGG GCACGGTTCT GGATCAATTA CATAAATCTG GGTTCCGTTG CCTATGCCCA
  841 GGTAGTATGG CGGTGGGTAC ATGAGCTCCA CCTTGCAGAT GTAGAGTCCC GTGTCCATGG
  901 CCCTCAGTCC TTGGATAGTG AGGTTCACTT GATTTCCACT GGAGGTGCCC GTGCAGATGG
  961 AATCATCTAG GAAGGTCAAC TCATTCCCCA TCATGTAGGT TGCCCGCACA GACTTCAGTC
 1021 ACCTGGCTGT CAGCCCTGCC GAAGCACTGT CACCCGGACC TCAGTGCTTT GCCTGAGATG
 1081 CATACTCACA CACAAGGCTG GGCGATGCTC GGCTGCTGGC AGGTTACACA GCAGCTTGGC
1141 ACGGGTGCAT TTGACAATGC AACT
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.11

GNP_filter3_RDBDEP_html_181012.pl