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cDNA library of mixed-stage C. elegans for yeast two-hybrid screening

cDNA library of mixed-stage C. elegans for yeast two-hybrid screening

Catalog number RDB01864
Resource name cDNA library of mixed-stage C. elegans for yeast two-hybrid screening
Clone info. mRNA derived from mixed-stage C. elegans N2 strain, cDNA primed with both oligo dT and random primer. The complexity of this library was 2 x 10^6 and an average insert size was 1.8 kb at establishment.
Provided as 1mL bacterial glycerol stock. A randomly picked lot of frozen stock had 1 to 2 x 10^8 colonies per mL in December 2018.
Vector backbone pGAD10 (Plasmid)
Size of vector backbone 6.6 kb
Selectable markers Amp^r (E. coli)
Gene/insert name C. elegans anonymous cDNA
Depositor Fujikawa, Noboru | Doi, Hirofumi | Terasaki, Asako |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR This material is provided with written consent of Dr. Hirofumi Doi (Developer). Acknowledgement to Developers in publication.
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Remarks ((Additional form)) Approval Form(FormD)
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Electronic file

Electronic file Map RDB01864.gif from Depositor

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References

original Terasaki, A.G., Searching for novel centrosomal components of C. elegans by two-hybrid analysis. Abstracts for 20th annual meeting of Mol. Biol. Society of Japan, 2JP435 (1997).

User reports and related articles (go to bottom)


Sequence information


References

References

original Terasaki, A.G., Searching for novel centrosomal components of C. elegans by two-hybrid analysis. Abstracts for 20th annual meeting of Mol. Biol. Society of Japan, 2JP435 (1997).
user report Underwood, J.G., Homologues of the Caenorhabditis elegans Fox-1 protein are neuronal splicing regulators in mammals. Mol. Cell. Biol., 25 (22), 10005-10016 (2005). PMID 16260614.
user report Nonomura Y., Involvement of fatty acid pathways and cortical interaction of the pronuclear complex in Caenorhabditis elegans embryonic polarity. BMC Dev. Biol., 3, 8 (2003). PMID 14527340.

2019.03.08

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