Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

cDNA library of mixed-stage C. elegans for yeast two-hybrid screening

cDNA library of mixed-stage C. elegans for yeast two-hybrid screening

Catalog number RDB01864
Resource name cDNA library of mixed-stage C. elegans for yeast two-hybrid screening
Clone info. mRNA derived from mixed-stage C. elegans N2 strain, cDNA primed with both oligo dT and random primer. The complexity of this library was 2 x 10^6 and an average insert size was 1.8 kb at establishment.
Comment Provided as 1mL bacterial glycerol stock. A randomly picked lot of frozen stock had 1 to 2 x 10^8 colonies per mL in December 2018.
Vector backbone pGAD10 (Plasmid)
Size of vector backbone 6.6 kb
Selectable markers Amp^r (E. coli)
Gene/insert name C. elegans anonymous cDNA
Depositor|Developer Fujikawa, Noboru | Doi, Hirofumi | Terasaki, Asako |
Other clones in our bank
External Database
C. elegans anonymous
            Reference sequence
              
             
            Remarks, protocol and/or map (gif) RDB01864.gif

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution This material is provided with written consent of Dr. Hirofumi Doi (Developer). Acknowledgement to Developers in publication.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form             [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form by depositor [Word]
            Remarks ((Additional form)) Approval Form(FormD)
            Please obtain written approval of the depositor.
            提供案内 (日本国内) [open/close]

            提供条件 樹立者土居洋文に許可をとること。研究結果の発表時、謝辞に樹立者名 土居洋文ならびに寺崎朝子を入れること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            寄託者による提供承諾書 [Word]
            備考 【追加提供依頼書】承諾書(FormD)
            組換え体提供にかかる書式が必要です。
            寄託者の書面による提供承諾が必要です。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB01864 cDNA library of mixed-stage C. elegans for yeast two-hybrid screening Under QC test. Please contact us. Please contact us. Please contact us.

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            How to cite this biological resource

            Materials & Methods section:

            The cDNA library of mixed-stage C. elegans for yeast two-hybrid screening was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01864).

            Reference section:

            Terasaki, A.G., Kishii, M., Kanai, A., Doi, H., Searching for novel centrosomal components of C. elegans by two-hybrid analysis. Abstracts for 20th annual meeting of Mol. Biol. Society of Japan, 2JP435 (1997).

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

            QC test results

            References

            Original, user report and related articles

            original Terasaki, A.G., Searching for novel centrosomal components of C. elegans by two-hybrid analysis. Abstracts for 20th annual meeting of Mol. Biol. Society of Japan, 2JP435 (1997).
            user_report Underwood, J.G., Homologues of the Caenorhabditis elegans Fox-1 protein are neuronal splicing regulators in mammals. Mol. Cell. Biol., 25 (22), 10005-10016 (2005). PMID 16260614.
            user_report Nonomura Y., Involvement of fatty acid pathways and cortical interaction of the pronuclear complex in Caenorhabditis elegans embryonic polarity. BMC Dev. Biol., 3, 8 (2003). PMID 14527340.

            2023.05.12

            GNP_filter3_RDBDEP_html_230512.pl