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pCALNL5 (#RDB01862)

A cassette to introduce cDNA into mammalian chromosome

Alternative name Up version of pCALNLw
Clone info. A cassette to introduce cDNA into mammalian chromosome under OFF-expression condition, which can be switched ON by the Cre-expressing adenovirus
Vector backbone pCALNL5 (Plasmid)
Size of vector backbone 6,098 bp
Selectable markers Amp^r (E. coli)
Gene/insert name E. coli neo Genomic DNA
Depositor|Developer Saito, Izumu |
 
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Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01862 pCALNL5 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCALNL5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01862).

Reference section:

Kanegae, Y., Takamori, K., Sato, Y., Lee, G., Nakai, M., Saito, I., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332. [link to RRC of NBRP]
Kanegae, Y., Lee, G., Sato, Y., Tanaka, M., Nakai, M., Sakaki, T., Sugano, S., Saito, I., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19245_B1Iap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CAG_R1 (Pr0584)
Region: CAG pro,lox P,NeoR 5'
Sequence file: RDB19245_B1Iaa.seq check
>01862_19245_B1Ia_3_CAG_R1_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGG CGCGGGGNNN NNNGGTGCGG CGCCGGCAGG
   61 AAGGAAATGG GCGGGGAGGG CCTTCGTGCG TCGCCGCGCC GCCGTCCCCT TCTCCCTCTC
  121 CAGCCTCGGG GCTGTCCGCG GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG
  181 TTCGGCTTCT GGCGTGTGAC CGGCGGCTCT AGAGCCTCTG CTAACCATGT TCATGCCTTC
  241 TTCTTTTTCC TACAGCTCCT GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC
  301 AAAGAATTGA TTAATTCGAG CGAACGCGTA TAACTTCGTA TAGCATACAT TATACGAAGT
  361 TATCTCGAGT CGGATTTGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA
  421 ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA
  481 CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCANGG
  541 GCGCCCGGTT CTTTTTGTCN AGANCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA
  601 GGCANCGCGG CTATCGTGGC TGGNCACGAC NGGCGTNCCT TGCGCAGCTG TGCTCGACGT
  661 TGTCNNTGAA NCGGGNANGG NACTGNNTGC TANTNNNGAA NNNNNNGGNN NNNNCNCCNG
721 NNNTCTNNNN NNNNNN
//
Primer: pCA-bgter (Pr0066)
Region: lox P,SV40 pA,NeoR 3'
Sequence file: RDB19245_B1Iab.seq check
>01862_19245_B1Ia_3_pCA-bgter_F01_06_ABI08.ab1
    1 NNNNNTNNTN TNNNNNNNNN NNNNNNNNNN NNCTCAGTGG TATTTGTNNN GCCAGGGCAT
   61 TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC CTGAGGAGTG AATTGATTCC
  121 CGGGTACCGA GCTCGAATTC AATTTAAATC GTACCGAGCG ACTCGAGATA ACTTCGTATA
  181 ATGTATGCTA TACGAAGTTA TACGCGTTCG GATTGATCCA GACATGATAA GATACATTGA
  241 TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG
  301 TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA TCGATGGGGT
  361 GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA
  421 AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG
  481 GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC
  541 GTCAAGAAGG CGATAGAANG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC
  601 GANGAANCGG TCAGCCCATT CGCCGCCAAG CTCTTNNNNN ATATCACGGG NANCCNANGC
661 TATGNCCTGN NNGCNGTNNN NNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,SV40 pA,ori
Sequence file: RDB19245_B1Iac.seq check
>01862_19245_B1Ia_3_SV40pro_ori_F_G01_07_ABI08.ab1
    1 NNNNNNNCNN NNNNNNGCCT CNNCCTCTGA NCTATTCNNG AAGTAGTGAG GAGGCTTTTT
   61 TGGAGGCCTA GGCTTTTGCA AAAAGCTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA
  121 TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT
  181 GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA TCCGCTGCAT TAATGAATCG
  241 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG
  301 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA
  361 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC
  421 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC
  481 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT
  541 AAAGATACCA GGCGTTTCCC CCTGNAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC
  601 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCNGGAAG CGTGGCGCTT TCTCNTAGCT
  661 CACGCTGNNN NTATCTCANN TCNGNGNNNN CNNNCGCTNN NNGNNNGGNT NNGNGNNCNA
721 NCCCCNNNNN NNNCNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332. [link to RRC of NBRP]
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]
reference Miyazaki, T., Calpastatin counteracts pathological angiogenesis by inhibiting suppressor of cytokine signaling 3 degradation in vascular endothelial cells. Circ. Res. 116 (7): 1170-1181 (2015). PMID 25648699.
reference Yamamoto, K., The role of group IIF-secreted phospholipase A2 in epidermal homeostasis and hyperplasia. J. Exp. Med. 212 (11): 1901-1919 (2015). PMID 26438362.
reference Hu, Z., Novel cell-based hepatitis C virus infection assay for quantitative high-throughput screening of anti-hepatitis C virus compounds. Antimicrob. Agents Chemother. 58 (2): 995-1004 (2014). PMID 24277038.
reference Kimura, T., Evidence that dendritic mitochondria negatively regulate dendritic branching in pyramidal neurons in the neocortex. J. Neurosci. 34 (20): 6938-6951 (2014). PMID 24828647.
reference Kanazawa, H., Heart failure causes cholinergic transdifferentiation of cardiac sympathetic nerves via gp130-signaling cytokines in rodents. J. Clin. Invest., 120 (2): 408-421 (2010). PMID 20051627. [link to RRC of NBRP]
reference Itano, N., Conditional Transgenic Mouse of Hyaluronan Synthase: A Potential Model of Advanced Breast Cancer. Experimental Glycoscience, 252-254 (2008). [link to RRC of NBRP]
reference Matsuda, T., Controlled expression of transgenes introduced by in vivo electroporation. Proc. Natl. Acad. Sci. U.S.A. 104 (3): 1027-1032 (2007). PMID 17209010. [link to RRC of NBRP]
reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837. [link to RRC of NBRP]
user_report Tachida, Y., O-GalNAc glycosylation determines intracellular trafficking of APP and Aβ production. J. Biol. Chem. 299 (7): 104905 (2023). PMID 37302553. [link to RRC of NBRP]
user_report Nakashiba, T., Development of two mouse strains conditionally expressing bright luciferases with distinct emission spectra as new tools for in vivo imaging. Lab. Anim. (NY). 2023 Sep 7. doi: 10.1038/s41684-023-01238-6. Epub ahead of print. PMID 37679611. [link to RRC of NBRP]
user_report Tachida, Y., Endothelial expression of human amyloid precursor protein leads to amyloid β in the blood and induces cerebral amyloid angiopathy in knock-in mice. J. Biol. Chem. 298 (6): 101880 (2022). PMID 35367207. [link to RRC of NBRP]
user_report Shibata, W., Overexpression of HER2 in the pancreas promotes development of intraductal papillary mucinous neoplasms in mice. Sci. Rep. 8 (1): 6150 (2018). PMID 29670173. [link to RRC of NBRP]
user_report Hashimoto, O., Activin E Controls Energy Homeostasis in Both Brown and White Adipose Tissues as a Hepatokine. Cell Rep. 25 (5): 1193-1203 (2018). PMID 30380411. [link to RRC of NBRP]
user_report Hata, S., A Gastrointestinal Calpain Complex, G-calpain, Is a Heterodimer of CAPN8 and CAPN9 Calpain Isoforms, Which Play Catalytic and Regulatory Roles, Respectively. J. Biol. Chem. 291 (53): 27313-27322 (2016). PMID 27881674. [link to RRC of NBRP]
user_report Katsuno, H., Actin Migration Driven by Directional Assembly and Disassembly of Membrane-Anchored Actin Filaments. Cell Rep. 12 (4): 648-660 (2015). PMID 26190109. [link to RRC of NBRP]
user_report Sakai, Y., A novel transgenic mouse model carrying human Tribbles related protein 3 (TRB3) gene and its site specific phenotype. Biol. Pharm. Bull., 37 (6): 1068-1074 (2014). PMID 24882419. [link to RRC of NBRP]
user_report Hatanaka, Y., Excitatory cortical neurons with multipolar shape establish neuronal polarity by forming a tangentially oriented axon in the intermediate zone. Cereb. Cortex, 23 (1): 105-113 (2013). PMID 22267309. [link to RRC of NBRP]
user_report Shimamura, M., Postnatal expression of BRAFV600E does not induce thyroid cancer in mouse models of thyroid papillary carcinoma. Endocrinology, 154 (11): 4423-4430 (2013). PMID 23970782. [link to RRC of NBRP]
user_report Braeuning, A., Coordinate regulation of cytochrome P450 1a1 expression in mouse liver by the aryl hydrocarbon receptor and the beta-catenin pathway. Toxicol .Sci., 122 (1): 16-25 (2011). PMID 21498875. [link to RRC of NBRP]
user_report Schreiber, S., Phenotype of single hepatocytes expressing an activated version of beta-catenin in liver of transgenic mice. J. Mol. Histol., 42 (5): 393-400 (2011). PMID 21822615. [link to RRC of NBRP]
user_report Nishimura, I., RB1CC1 suppresses type II collagen synthesis in chondrocytes, and causes dwarfism. J. Biol. Chem., 286 (51): 43925-43932 (2011). PMID 22049074. [link to RRC of NBRP]
user_report Tanaka H., Mature acinar cells are refractory to carcinoma development by targeted activation of Ras oncogene in adult rats. Cancer Sci., 101, 341-346 (2010). PMID 19917056. [link to RRC of NBRP]
user_report Fukamachi K., An animal model of preclinical diagnosis of pancreatic ductal adenocarcinomas. Biochem. Biophys. Res. Commun., 390, 636-641 (2009). PMID 19818733. [link to RRC of NBRP]
user_report McMahon, H.E., Oocyte-specific overexpression of mouse bone morphogenetic protein-15 leads to accelerated folliculogenesis and an early onset of acyclicity in transgenic mice. Endocrinology, 149, 2807-2815 (2008). PMID 18308851. [link to RRC of NBRP]
user_report Kohyama J., Epigenetic regulation of neural cell differentiation plasticity in the adult mammalian brain. Proc. Natl. Acad. Sci. U. S. A., 105, 18012-18017 (2008). PMID 19004774. [link to RRC of NBRP]
user_report Kaartinen V., TGFbeta3-induced activation of RhoA/Rho-kinase pathway is necessary but not sufficient for epithelio-mesenchymal transdifferentiation: implications for palatogenesis. Int. J. Mol. Med., 9, 563-70 (2002). PMID 12011971. [link to RRC of NBRP]