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pCALNL5

A cassette to introduce cDNA into mammalian chromosome

Catalog number RDB01862
Resource name pCALNL5
Alternative name Up version of pCALNLw
Clone info. A cassette to introduce cDNA into mammalian chromosome under OFF-expression condition, which can be switched ON by the Cre-expressing adenovirus
Vector backbone pCALNL5 (Plasmid)
Size of vector backbone 6,098 bp
Selectable markers Amp^r (E. coli)
Gene/insert name E. coli neo Genomic DNA
Depositor Saito, Izumu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01862 pCALNL5 DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB01862_A3Frp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB01862_A3Fra.seq
>01862_01862_A3Fr_3_pAxCAF1_A08_02.ab1
    1 TCTTCAACGA GTGGTCGCTC ATGTTCATGC CTTCTTCTTT TTCCTACAGC TCCTGGGCAA
   61 CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTGATTAATT CGAGCGAACG
  121 CGTATAACTT CGTATAGCAT ACATTATACG AAGTTATCTC GAGTCGGATT TGATCTGATC
  181 AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC
  241 CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT
  301 CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG
  361 ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA
  421 CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC
  481 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA
  541 AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC
  601 CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC
  661 TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG
  721 CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT
  781 GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTTCTG GATTCATCGA CTGTGGCCGG
  841 CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG
  901 CTTGGCGGCG AAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTTCCCGATT
  961 CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT TCTGAGCGGG GACTTCTGGG
 1021 GGTTCGAAAT GACCCGACCC AGCGACGCCC AAACCCTGCC CAATCACGAG ATTTCCGATT
1081 CAACGGTCGT CCTTCTTATG GACAGGTTGG GGCGTTTCCC GG
//
Primer: Reverse2 RDB01862_A3Frb.seq
>01862_01862_A3Fr_3_Reverse2_B08_05.ab1
    1 CATTAAATCA TTACGCCAGC TTGGGCTGCA GGTCGAGGGA TCTCCATAAG AGAAGAGGGA
   61 CAGCTATGAC TGGGAGTAGT CAGGAGAGGA GGAAAAATCT GGCTAGTAAA ACATGTAAGG
  121 AAAATTTTAG GGATGTTAAA GAAAAAAATA ACACAAAACA AAATATAAAA AAAATCTAAC
  181 CTCAAGTCAA GGCTTTTCTA TGGAATAAGG AATGGACAGC AGGGGGCTGT TTCATATACT
  241 GATGACCTCT TTATAGCCAA CCTTTGTTCA TGGCAGCCAG CATATGGGCA TATGTTGCCA
  301 AACTCTAAAC CAAATACTCA TTCTGATGTT TTAAATGATT TGCCCTCCCA TATGTCCTTC
  361 CGAGTGAGAG ACACAAAAAA TTCCAACACA CTATTGCAAT GAAAATAAAT TTCCTTTATT
  421 AGCCAGAAGT CAGATGCTCA AGGGGCTTCA TGATGTCCCC ATAATTTTTG GCAGAGGGAA
  481 AAAGATCTCA GTGGTATTTG TGAGCCAGGG CATTGGCCAC ACCAGCCACC ACCTTCTGAT
  541 AGGCAGCCTG CACCTGAGGA GTGAATTGAT TCCCGGGTAC CGAGCTCGAA TTCAATTTAA
  601 ATCGTACCGA GCGACTCGAG ATAACTTCGT ATAATGTATG CTATACGAAG TTATACGCGT
  661 TCGGATTGAT CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT
  721 GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT
  781 TATAAGCTGC AATAAACAAG TTATCGATGG GGTGGGCGAA GAACTCCAGC ATGAGATCCC
  841 CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT
  901 AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA
  961 TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG
 1021 CCTGCGAATC GGGAGCGGGC GATACCGTAA GCACGGAGAA GCGGGTCAGC CAATCGCGGC
1081 TAGGCTCTTC CAGCCATATC ACGGGTAGCC CAACGTCTT
//

Please visit Sequencing and PCR primers for primer information.

References and tips

Electronic file

Electronic file Map RDB01862.gif from Depositor

Featured content

Featured content Cre-loxP and FLP-FRT System (English text)
Featured content Dnaconda's recommendation EXR0045e (English text)
Featured content Dnaconda's recommendation EXR0045j (Japanese text)
Featured content Control Vectors (English text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

References

original Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332.
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
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user report Tanaka H., Mature acinar cells are refractory to carcinoma development by targeted activation of Ras oncogene in adult rats. Cancer Sci., 101, 341-346 (2010). PMID 19917056.
user report Fukamachi K., An animal model of preclinical diagnosis of pancreatic ductal adenocarcinomas. Biochem. Biophys. Res. Commun., 390, 636-641 (2009). PMID 19818733.
user report McMahon, H.E., Oocyte-specific overexpression of mouse bone morphogenetic protein-15 leads to accelerated folliculogenesis and an early onset of acyclicity in transgenic mice. Endocrinology, 149, 2807-2815 (2008). PMID 18308851.
user report Kohyama J., Epigenetic regulation of neural cell differentiation plasticity in the adult mammalian brain. Proc. Natl. Acad. Sci. U. S. A., 105, 18012-18017 (2008). PMID 19004774.
user report Kaartinen V., TGFbeta3-induced activation of RhoA/Rho-kinase pathway is necessary but not sufficient for epithelio-mesenchymal transdifferentiation: implications for palatogenesis. Int. J. Mol. Med., 9, 563-70 (2002). PMID 12011971.

2018.05.25

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