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pCALNL5

A cassette to introduce cDNA into mammalian chromosome

Catalog number RDB01862
Resource name pCALNL5
Alternative name Up version of pCALNLw
Clone info. A cassette to introduce cDNA into mammalian chromosome under OFF-expression condition, which can be switched ON by the Cre-expressing adenovirus
Vector backbone pCALNL5 (Plasmid)
Size of vector backbone 6,098 bp
Selectable markers Amp^r (E. coli)
Gene/insert name E. coli neo Genomic DNA
Depositor|Developer Saito, Izumu |
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External Database
E. coli neo

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
            Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。
            付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB01862 pCALNL5 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Remarks, protocol and/or map (gif) RDB01862.gif

            Original reference

            original Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332.
            original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Dnaconda's recommendation EXR0045e (English text)
            Featured content Dnaconda's recommendation EXR0045j (Japanese text)
            Featured content Control Vectors (English text)
            Featured content Cre-loxP and FLP-FRT System (English text)
            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB19245_B1Iap1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CAG_R1
            Region: CAG pro,lox P,NeoR 5'
            Sequence file: RDB19245_B1Iaa.seq check
            >01862_19245_B1Ia_3_CAG_R1_E01_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGG CGCGGGGNNN NNNGGTGCGG CGCCGGCAGG
               61 AAGGAAATGG GCGGGGAGGG CCTTCGTGCG TCGCCGCGCC GCCGTCCCCT TCTCCCTCTC
              121 CAGCCTCGGG GCTGTCCGCG GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG
              181 TTCGGCTTCT GGCGTGTGAC CGGCGGCTCT AGAGCCTCTG CTAACCATGT TCATGCCTTC
              241 TTCTTTTTCC TACAGCTCCT GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC
              301 AAAGAATTGA TTAATTCGAG CGAACGCGTA TAACTTCGTA TAGCATACAT TATACGAAGT
              361 TATCTCGAGT CGGATTTGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA
              421 ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA
              481 CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCANGG
              541 GCGCCCGGTT CTTTTTGTCN AGANCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA
              601 GGCANCGCGG CTATCGTGGC TGGNCACGAC NGGCGTNCCT TGCGCAGCTG TGCTCGACGT
              661 TGTCNNTGAA NCGGGNANGG NACTGNNTGC TANTNNNGAA NNNNNNGGNN NNNNCNCCNG
            721 NNNTCTNNNN NNNNNN
            //
            Primer: pCA-bgter
            Region: lox P,SV40 pA,NeoR 3'
            Sequence file: RDB19245_B1Iab.seq check
            >01862_19245_B1Ia_3_pCA-bgter_F01_06_ABI08.ab1
                1 NNNNNTNNTN TNNNNNNNNN NNNNNNNNNN NNCTCAGTGG TATTTGTNNN GCCAGGGCAT
               61 TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC CTGAGGAGTG AATTGATTCC
              121 CGGGTACCGA GCTCGAATTC AATTTAAATC GTACCGAGCG ACTCGAGATA ACTTCGTATA
              181 ATGTATGCTA TACGAAGTTA TACGCGTTCG GATTGATCCA GACATGATAA GATACATTGA
              241 TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG
              301 TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA TCGATGGGGT
              361 GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA
              421 AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG
              481 GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC
              541 GTCAAGAAGG CGATAGAANG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC
              601 GANGAANCGG TCAGCCCATT CGCCGCCAAG CTCTTNNNNN ATATCACGGG NANCCNANGC
            661 TATGNCCTGN NNGCNGTNNN NNNNNNN
            //
            Primer: SV40pro_ori_F
            Region: SV40 pro_ori,SV40 pA,ori
            Sequence file: RDB19245_B1Iac.seq check
            >01862_19245_B1Ia_3_SV40pro_ori_F_G01_07_ABI08.ab1
                1 NNNNNNNCNN NNNNNNGCCT CNNCCTCTGA NCTATTCNNG AAGTAGTGAG GAGGCTTTTT
               61 TGGAGGCCTA GGCTTTTGCA AAAAGCTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA
              121 TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT
              181 GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA TCCGCTGCAT TAATGAATCG
              241 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG
              301 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA
              361 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC
              421 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC
              481 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT
              541 AAAGATACCA GGCGTTTCCC CCTGNAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC
              601 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCNGGAAG CGTGGCGCTT TCTCNTAGCT
              661 CACGCTGNNN NTATCTCANN TCNGNGNNNN CNNNCGCTNN NNGNNNGGNT NNGNGNNCNA
            721 NCCCCNNNNN NNNCNNNNNN NNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332.
            original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
            reference Miyazaki, T., Calpastatin counteracts pathological angiogenesis by inhibiting suppressor of cytokine signaling 3 degradation in vascular endothelial cells. Circ. Res. 116 (7): 1170-1181 (2015). PMID 25648699.
            reference Yamamoto, K., The role of group IIF-secreted phospholipase A2 in epidermal homeostasis and hyperplasia. J. Exp. Med. 212 (11): 1901-1919 (2015). PMID 26438362.
            reference Hu, Z., Novel cell-based hepatitis C virus infection assay for quantitative high-throughput screening of anti-hepatitis C virus compounds. Antimicrob. Agents Chemother. 58 (2): 995-1004 (2014). PMID 24277038.
            reference Kimura, T., Evidence that dendritic mitochondria negatively regulate dendritic branching in pyramidal neurons in the neocortex. J. Neurosci. 34 (20): 6938-6951 (2014). PMID 24828647.
            reference Kanazawa, H., Heart failure causes cholinergic transdifferentiation of cardiac sympathetic nerves via gp130-signaling cytokines in rodents. J. Clin. Invest., 120 (2): 408-421 (2010). PMID 20051627.
            reference Itano, N., Conditional Transgenic Mouse of Hyaluronan Synthase: A Potential Model of Advanced Breast Cancer. Experimental Glycoscience, 252-254 (2008).
            reference Matsuda, T., Controlled expression of transgenes introduced by in vivo electroporation. Proc. Natl. Acad. Sci. U.S.A. 104 (3): 1027-1032 (2007). PMID 17209010.
            reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837.
            user report Shibata, W., Overexpression of HER2 in the pancreas promotes development of intraductal papillary mucinous neoplasms in mice. Sci. Rep. 8 (1): 6150 (2018). PMID 29670173.
            user report Hashimoto, O., Activin E Controls Energy Homeostasis in Both Brown and White Adipose Tissues as a Hepatokine. Cell Rep. 25 (5): 1193-1203 (2018). PMID 30380411.
            user report Hata, S., A Gastrointestinal Calpain Complex, G-calpain, Is a Heterodimer of CAPN8 and CAPN9 Calpain Isoforms, Which Play Catalytic and Regulatory Roles, Respectively. J. Biol. Chem. 291 (53): 27313-27322 (2016). PMID 27881674.
            user report Katsuno, H., Actin Migration Driven by Directional Assembly and Disassembly of Membrane-Anchored Actin Filaments. Cell Rep. 12 (4): 648-660 (2015). PMID 26190109.
            user report Sakai, Y., A novel transgenic mouse model carrying human Tribbles related protein 3 (TRB3) gene and its site specific phenotype. Biol. Pharm. Bull., 37 (6): 1068-1074 (2014). PMID 24882419.
            user report Hatanaka, Y., Excitatory cortical neurons with multipolar shape establish neuronal polarity by forming a tangentially oriented axon in the intermediate zone. Cereb. Cortex, 23 (1): 105-113 (2013). PMID 22267309.
            user report Shimamura, M., Postnatal expression of BRAFV600E does not induce thyroid cancer in mouse models of thyroid papillary carcinoma. Endocrinology, 154 (11): 4423-4430 (2013). PMID 23970782.
            user report Braeuning, A., Coordinate regulation of cytochrome P450 1a1 expression in mouse liver by the aryl hydrocarbon receptor and the beta-catenin pathway. Toxicol .Sci., 122 (1): 16-25 (2011). PMID 21498875.
            user report Schreiber, S., Phenotype of single hepatocytes expressing an activated version of beta-catenin in liver of transgenic mice. J. Mol. Histol., 42 (5): 393-400 (2011). PMID 21822615.
            user report Nishimura, I., RB1CC1 suppresses type II collagen synthesis in chondrocytes, and causes dwarfism. J. Biol. Chem., 286 (51): 43925-43932 (2011). PMID 22049074.
            user report Tanaka H., Mature acinar cells are refractory to carcinoma development by targeted activation of Ras oncogene in adult rats. Cancer Sci., 101, 341-346 (2010). PMID 19917056.
            user report Fukamachi K., An animal model of preclinical diagnosis of pancreatic ductal adenocarcinomas. Biochem. Biophys. Res. Commun., 390, 636-641 (2009). PMID 19818733.
            user report McMahon, H.E., Oocyte-specific overexpression of mouse bone morphogenetic protein-15 leads to accelerated folliculogenesis and an early onset of acyclicity in transgenic mice. Endocrinology, 149, 2807-2815 (2008). PMID 18308851.
            user report Kohyama J., Epigenetic regulation of neural cell differentiation plasticity in the adult mammalian brain. Proc. Natl. Acad. Sci. U. S. A., 105, 18012-18017 (2008). PMID 19004774.
            user report Kaartinen V., TGFbeta3-induced activation of RhoA/Rho-kinase pathway is necessary but not sufficient for epithelio-mesenchymal transdifferentiation: implications for palatogenesis. Int. J. Mol. Med., 9, 563-70 (2002). PMID 12011971.

            2022.02.09

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