icon below as well as clone information before placing your order.
A cassette to introduce cDNA into mammalian chromosome
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External Database E. coli neo Reference sequence |
| Remarks, protocol and/or map (gif) | RDB01862.gif
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)). Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested. |
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| Ordering | Order form [Credit Card Payment  ] [Bank Transfer Payment]Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ]Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
[open/close]| 提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。 |
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| 提供依頼 | 提供依頼書 [Word ]提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ]手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB01862 | pCALNL5 | DNA solution |
icon below as well as clone information before placing your order.Materials & Methods section:
| The pCALNL5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01862). |
Reference section:
| Kanegae, Y., Takamori, K., Sato, Y., Lee, G., Nakai, M., Saito, I., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332. [link to RRC of NBRP] |
| Kanegae, Y., Lee, G., Sato, Y., Tanaka, M., Nakai, M., Sakaki, T., Sugano, S., Saito, I., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Dnaconda's recommendation EXR0045e (English text) |
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| Featured content | Dnaconda's recommendation EXR0045j (Japanese text) |
| Featured content | Empty Backbone (English text) |
| Featured content | Empty Backbone (Japanese text) |
| Featured content | Expression regulation (English text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB19245_B1Iap1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: CAG_R1 (Pr0584) Region: CAG pro,lox P,NeoR 5' Sequence file: RDB19245_B1Iaa.seq
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>01862_19245_B1Ia_3_CAG_R1_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGG CGCGGGGNNN NNNGGTGCGG CGCCGGCAGG 61 AAGGAAATGG GCGGGGAGGG CCTTCGTGCG TCGCCGCGCC GCCGTCCCCT TCTCCCTCTC 121 CAGCCTCGGG GCTGTCCGCG GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG 181 TTCGGCTTCT GGCGTGTGAC CGGCGGCTCT AGAGCCTCTG CTAACCATGT TCATGCCTTC 241 TTCTTTTTCC TACAGCTCCT GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC 301 AAAGAATTGA TTAATTCGAG CGAACGCGTA TAACTTCGTA TAGCATACAT TATACGAAGT 361 TATCTCGAGT CGGATTTGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA 421 ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA 481 CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCANGG 541 GCGCCCGGTT CTTTTTGTCN AGANCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA 601 GGCANCGCGG CTATCGTGGC TGGNCACGAC NGGCGTNCCT TGCGCAGCTG TGCTCGACGT 661 TGTCNNTGAA NCGGGNANGG NACTGNNTGC TANTNNNGAA NNNNNNGGNN NNNNCNCCNG 721 NNNTCTNNNN NNNNNN // |
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Primer: pCA-bgter (Pr0066) Region: lox P,SV40 pA,NeoR 3' Sequence file: RDB19245_B1Iab.seq
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>01862_19245_B1Ia_3_pCA-bgter_F01_06_ABI08.ab1 1 NNNNNTNNTN TNNNNNNNNN NNNNNNNNNN NNCTCAGTGG TATTTGTNNN GCCAGGGCAT 61 TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC CTGAGGAGTG AATTGATTCC 121 CGGGTACCGA GCTCGAATTC AATTTAAATC GTACCGAGCG ACTCGAGATA ACTTCGTATA 181 ATGTATGCTA TACGAAGTTA TACGCGTTCG GATTGATCCA GACATGATAA GATACATTGA 241 TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG 301 TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA TCGATGGGGT 361 GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA 421 AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG 481 GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC 541 GTCAAGAAGG CGATAGAANG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC 601 GANGAANCGG TCAGCCCATT CGCCGCCAAG CTCTTNNNNN ATATCACGGG NANCCNANGC 661 TATGNCCTGN NNGCNGTNNN NNNNNNN // |
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Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,SV40 pA,ori Sequence file: RDB19245_B1Iac.seq
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>01862_19245_B1Ia_3_SV40pro_ori_F_G01_07_ABI08.ab1 1 NNNNNNNCNN NNNNNNGCCT CNNCCTCTGA NCTATTCNNG AAGTAGTGAG GAGGCTTTTT 61 TGGAGGCCTA GGCTTTTGCA AAAAGCTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA 121 TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT 181 GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA TCCGCTGCAT TAATGAATCG 241 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 301 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 361 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 421 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 481 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 541 AAAGATACCA GGCGTTTCCC CCTGNAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 601 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCNGGAAG CGTGGCGCTT TCTCNTAGCT 661 CACGCTGNNN NTATCTCANN TCNGNGNNNN CNNNCGCTNN NNGNNNGGNT NNGNGNNCNA 721 NCCCCNNNNN NNNCNNNNNN NNNN // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332. [link to RRC of NBRP] |
|---|---|
| original | Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP] |
| reference | Miyazaki, T., Calpastatin counteracts pathological angiogenesis by inhibiting suppressor of cytokine signaling 3 degradation in vascular endothelial cells. Circ. Res. 116 (7): 1170-1181 (2015). PMID 25648699. |
| reference | Yamamoto, K., The role of group IIF-secreted phospholipase A2 in epidermal homeostasis and hyperplasia. J. Exp. Med. 212 (11): 1901-1919 (2015). PMID 26438362. |
| reference | Hu, Z., Novel cell-based hepatitis C virus infection assay for quantitative high-throughput screening of anti-hepatitis C virus compounds. Antimicrob. Agents Chemother. 58 (2): 995-1004 (2014). PMID 24277038. |
| reference | Kimura, T., Evidence that dendritic mitochondria negatively regulate dendritic branching in pyramidal neurons in the neocortex. J. Neurosci. 34 (20): 6938-6951 (2014). PMID 24828647. |
| reference | Kanazawa, H., Heart failure causes cholinergic transdifferentiation of cardiac sympathetic nerves via gp130-signaling cytokines in rodents. J. Clin. Invest., 120 (2): 408-421 (2010). PMID 20051627. [link to RRC of NBRP] |
| reference | Itano, N., Conditional Transgenic Mouse of Hyaluronan Synthase: A Potential Model of Advanced Breast Cancer. Experimental Glycoscience, 252-254 (2008). [link to RRC of NBRP] |
| reference | Matsuda, T., Controlled expression of transgenes introduced by in vivo electroporation. Proc. Natl. Acad. Sci. U.S.A. 104 (3): 1027-1032 (2007). PMID 17209010. [link to RRC of NBRP] |
| reference | Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837. [link to RRC of NBRP] |
| user_report | Tachida, Y., O-GalNAc glycosylation determines intracellular trafficking of APP and Aβ production. J. Biol. Chem. 299 (7): 104905 (2023). PMID 37302553. [link to RRC of NBRP] |
| user_report | Nakashiba, T., Development of two mouse strains conditionally expressing bright luciferases with distinct emission spectra as new tools for in vivo imaging. Lab. Anim. (NY). 2023 Sep 7. doi: 10.1038/s41684-023-01238-6. Epub ahead of print. PMID 37679611. [link to RRC of NBRP] |
| user_report | Tachida, Y., Endothelial expression of human amyloid precursor protein leads to amyloid β in the blood and induces cerebral amyloid angiopathy in knock-in mice. J. Biol. Chem. 298 (6): 101880 (2022). PMID 35367207. [link to RRC of NBRP] |
| user_report | Shibata, W., Overexpression of HER2 in the pancreas promotes development of intraductal papillary mucinous neoplasms in mice. Sci. Rep. 8 (1): 6150 (2018). PMID 29670173. [link to RRC of NBRP] |
| user_report | Hashimoto, O., Activin E Controls Energy Homeostasis in Both Brown and White Adipose Tissues as a Hepatokine. Cell Rep. 25 (5): 1193-1203 (2018). PMID 30380411. [link to RRC of NBRP] |
| user_report | Hata, S., A Gastrointestinal Calpain Complex, G-calpain, Is a Heterodimer of CAPN8 and CAPN9 Calpain Isoforms, Which Play Catalytic and Regulatory Roles, Respectively. J. Biol. Chem. 291 (53): 27313-27322 (2016). PMID 27881674. [link to RRC of NBRP] |
| user_report | Katsuno, H., Actin Migration Driven by Directional Assembly and Disassembly of Membrane-Anchored Actin Filaments. Cell Rep. 12 (4): 648-660 (2015). PMID 26190109. [link to RRC of NBRP] |
| user_report | Sakai, Y., A novel transgenic mouse model carrying human Tribbles related protein 3 (TRB3) gene and its site specific phenotype. Biol. Pharm. Bull., 37 (6): 1068-1074 (2014). PMID 24882419. [link to RRC of NBRP] |
| user_report | Hatanaka, Y., Excitatory cortical neurons with multipolar shape establish neuronal polarity by forming a tangentially oriented axon in the intermediate zone. Cereb. Cortex, 23 (1): 105-113 (2013). PMID 22267309. [link to RRC of NBRP] |
| user_report | Shimamura, M., Postnatal expression of BRAFV600E does not induce thyroid cancer in mouse models of thyroid papillary carcinoma. Endocrinology, 154 (11): 4423-4430 (2013). PMID 23970782. [link to RRC of NBRP] |
| user_report | Braeuning, A., Coordinate regulation of cytochrome P450 1a1 expression in mouse liver by the aryl hydrocarbon receptor and the beta-catenin pathway. Toxicol .Sci., 122 (1): 16-25 (2011). PMID 21498875. [link to RRC of NBRP] |
| user_report | Schreiber, S., Phenotype of single hepatocytes expressing an activated version of beta-catenin in liver of transgenic mice. J. Mol. Histol., 42 (5): 393-400 (2011). PMID 21822615. [link to RRC of NBRP] |
| user_report | Nishimura, I., RB1CC1 suppresses type II collagen synthesis in chondrocytes, and causes dwarfism. J. Biol. Chem., 286 (51): 43925-43932 (2011). PMID 22049074. [link to RRC of NBRP] |
| user_report | Tanaka H., Mature acinar cells are refractory to carcinoma development by targeted activation of Ras oncogene in adult rats. Cancer Sci., 101, 341-346 (2010). PMID 19917056. [link to RRC of NBRP] |
| user_report | Fukamachi K., An animal model of preclinical diagnosis of pancreatic ductal adenocarcinomas. Biochem. Biophys. Res. Commun., 390, 636-641 (2009). PMID 19818733. [link to RRC of NBRP] |
| user_report | McMahon, H.E., Oocyte-specific overexpression of mouse bone morphogenetic protein-15 leads to accelerated folliculogenesis and an early onset of acyclicity in transgenic mice. Endocrinology, 149, 2807-2815 (2008). PMID 18308851. [link to RRC of NBRP] |
| user_report | Kohyama J., Epigenetic regulation of neural cell differentiation plasticity in the adult mammalian brain. Proc. Natl. Acad. Sci. U. S. A., 105, 18012-18017 (2008). PMID 19004774. [link to RRC of NBRP] |
| user_report | Kaartinen V., TGFbeta3-induced activation of RhoA/Rho-kinase pathway is necessary but not sufficient for epithelio-mesenchymal transdifferentiation: implications for palatogenesis. Int. J. Mol. Med., 9, 563-70 (2002). PMID 12011971. [link to RRC of NBRP] |
2023.09.13
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