Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pTZ331 (#RDB01804)

B. subtilis aadK genomic DNA

Alternative name aadK
Clone info. The chromosomal aadk gene coding for the aminoglycoside 6-adenylyltransferase [AAD (6)] from Bacillus subtilis Marburg 168
Vector backbone pACYC184 (Plasmid)
Size of vector backbone 4,244 bp
Selectable markers Cm^r
Gene/insert name Bacillus subtilis AadK Genomic DNA
Reference of insert sequence M26879 (DDBJ accession number)
Depositor|Developer Noguchi, Norihisa |
 
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form by depositor [Word]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution This material is provided with written consent of Depositor.
Remarks ((Additional form)) Approval Form(FormD)
Please obtain written approval of the depositor.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
寄託者による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 寄託者に許可をとること。
備考 【追加提供依頼書】承諾書(FormD)
寄託者の書面による提供承諾が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB01804 pTZ331 Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pTZ331 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01804).

Reference section:

Noguchi, N., Sasatsu, M., Kono, M., Genetic mapping in Bacillus subtilis 168 of the aadK gene which encodes aminoglycoside 6-adenylyltransferase. FEMS Microbiol. Lett., 114, 47-52 (1993). PMID 8293959. [link to RRC of NBRP]
Notuchi, N., Ohmiya, K., Tanaka, T., O’hara, K., Mono, M., Expression of the Aminoglycoside 6-Adenylyltransferase Coding Gene from Bacillus subtilis in Escherichia coli. Agric. Biol. Chem., 53, 2519-2520 (1989).
Ohmiya, K., Tanaka, T., Noguchi, N., O'Hara, K., Kono, M., Nucleotide sequence of the chromosomal gene coding for the aminoglycoside 6-adenylyltransferase from Bacillus subtilis Marburg 168. Gene, 78, 377-378 (1989). PMID 2550327. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Noguchi, N., Genetic mapping in Bacillus subtilis 168 of the aadK gene which encodes aminoglycoside 6-adenylyltransferase. FEMS Microbiol. Lett., 114, 47-52 (1993). PMID 8293959. [link to RRC of NBRP]
original Notuchi, N., Expression of the Aminoglycoside 6-Adenylyltransferase Coding Gene from Bacillus subtilis in Escherichia coli. Agric. Biol. Chem., 53, 2519-2520 (1989).
original Ohmiya, K., Nucleotide sequence of the chromosomal gene coding for the aminoglycoside 6-adenylyltransferase from Bacillus subtilis Marburg 168. Gene, 78, 377-378 (1989). PMID 2550327. [link to RRC of NBRP]