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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

RAT GATA-GT1 (#RDB01763)

Rat GATA-6 cDNA

Alternative name RAT GATA-6
Clone info. Zinc finger protein rat GATA-GT1
Vector backbone pUC18 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Rat GATA-GT1 cDNA
Reference of insert sequence L22760 (DDBJ accession number)
Depositor|Developer Futai, Masamitsu |
 
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RDB01763 RAT GATA-GT1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The RAT GATA-GT1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01763).

Reference section:

Tamura, S., Wang, X.H., Maeda, M., Futai, M., Gastric DNA-binding proteins recognize upstream sequence motifs of parietal cell-specific genes. Proc. Natl. Acad. Sci. USA, 90, 10876-10880 (1993). PMID 8248184. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16751_A8Jpp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2 ()
Sequence file: RDB16751_A8Jpa.seq check
>01763_16751_A8Jp_Reverse2_A01_01_ABI08.ab1
    1 ATTAAACGAA AATTCGCGGG CCCGGCCGAC TTGGGAGGAC CTGTTGCTCT TCACTGACCT
   61 CGATCAGGCC GCGACCGCCA GCAAGCTGCT GTGGTCCAGC CGGGGCGCCA AGCTGAGTCC
  121 CTTCGCGGCC GAGCAGCCGG AGGAAATGTA CCAGACGCTC GCCGCTCTGT CCAGCCAGGG
  181 GCCCGCCGCT TACGACGGCG CTCCCGGCGG CTTCGTACAC TCCGCAGCTG CGGCGGCCGC
  241 GGCGGCCGCG GCAGCCAGCT CTCCGGTCTA CGTGCCCACC ACACGCGTGG GCTCCATGCT
  301 GCCCGGCCTG CCTTACCTGC AGGGGGCGGG CAGCGGGCCC AGCAATCACG CGGGCGGCGC
  361 GGGCGCCCAC CCGGGCTGGC CCCAGGCCTC CGCCGACAGC CCCCCGTATG GCGGGGGTGG
  421 TGCAGCGGGC GGCGGCGCGG CCGGACCCGG AGGTGCGGGA TCAGCCACAG CCCACGCCTC
  481 TGCACGCTTC CCCTACTCGC CCAGCCCGCC CATGGCCAAC GGCGCCGCGC GGGACCCCGG
  541 GGGCTACGTG GCTGCGGGCG GCGCGGGCGC GGGTAGCGTG AGTGGCGGTG GCGGCAGCCT
  601 GGCGGCCATG GGTGGCCGGG AGCACCAGTA CAGCTCGCTG TCCGCAGCCA GGCCGCTGAA
  661 CGGGACGTAC CACCACCACC ATCATCACCA CCCGACCTAC TCGCCCTACA TGGGCGCTCC
  721 TCTGACTCCC GCCTGGCCAG CGGGACCCTT CGAAACGCCG GTGCTCCACA GTTTACAGAG
  781 CCGCGCGGGA GCTCCACTCC CGGTGCCACG AGGCCCCCAG CGCAGACCCT GTTGGAGGGA
  841 CCTGTCGGAG AGCCGCGAGT GGCGTGAACT GTGGGCTCCA TCCCAGACGC CAACTTGTGG
  901 GAAGACGGAG GATGGCACCG GGTCATTTAA CCTGTGGCAA ACGCATGGCG GGTCTTCTTA
  961 CAAGTTAAGG ATGGAACGGG CCTTCAGGCA GGGCCCCCTT CATCAAGGCC ACAGAAAGGC
1021 GCGGTGGCCC TTCATTCACC GACGGGGCTT GGACTTGGTT CCT
//
Primer: pUC-M13_-100 ()
Sequence file: RDB16751_A8Jpb.seq check
>01763_16751_A8Jp_pUC-M13_-100_C01_03_ABI08.ab1
    1 TTTTCCGGGA AACTTCCTAA GGAGGATCCC CGGGGTTATT CGAAGGCTCG AATTCGCGGC
   61 CGCAATCTCT CAATCTTCCT TAGCAGACAA GGCCCCAGTC ATTGTCACAG TTTCTCCCAC
  121 TGGTGTGGAG CAGAGCCAGA GGCAAGCTGG CTGGAAGCAG CCCAGGCTGA CAGGAATAGG
  181 CCACACGCCC ACTGTGCTGC CCCTTCCTAC AAAGACCCAT CTCTTCTTAC TTCAATAAGC
  241 TTCTTGGGAA ATATATCTCT TTCAAAACAA AGGCACAGAA ATCACGCATC GAAGGAATGT
  301 TATGTCTGCA TTTTTTGCCG CCATCTGGAC TGCTGGACAA AATCAGACAC AAGTGGTATG
  361 AGGCCTTCAG AGCCCTTGGC AGCACCAGCT CAGGCCAGGG CCAGAGCACA CCAAGAATCC
  421 TGTCGCACCG AGGATGTAAC TTCGGCAGGG GAGGACAAAC TGACACCTAT GTAGAGGCCA
  481 TCTTGACCTG AATACTTGAG GTCACTGTTC TCAGGGTTGG CACTTTCTCC CACTGCAGAC
  541 ATCACTGATG CACCCACCCC TGAGGCAGTC GACTGTGTGG GAGGAGAAGT ATTTTTGGTG
  601 CAGTCATCTG AATTAGAGGA GGAGGAAGTT GGAGTCATAG GAACAAGAGC TGTTACCCGG
  661 AGCCAAGCTT TCGACTTATT TATATTTTTA GGTTTTTCGT TTCCCTGGTT TGAATTCCTT
  721 CTTTTTTCAT AGCAAAGTGG GTCGAGGGCA CCCCATGGAG TTTCATATAA AGCCCCGGCA
781 AGCCTTTGCA CACGGGGCTC ACCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tamura, S., Gastric DNA-binding proteins recognize upstream sequence motifs of parietal cell-specific genes. Proc. Natl. Acad. Sci. USA, 90, 10876-10880 (1993). PMID 8248184. [link to RRC of NBRP]