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AxCALNLNZ

Recombinant adenovirus expressing E. coli lacZ

Catalog number RDB01750
Resource name AxCALNLNZ
Clone info. Rrecombinant adenovirus containing an expression-switched unit, initially expressing neo gene and then expressing beta-galactosidase tagged with a nuclear localization signal under the control of CAG promoter after Cre-mediated excision of the neo gene.
Vector backbone pAxcw (Adenovirus)
Size of vector backbone -
Growth remarks 5 percent FCS is fine.
Gene/insert name E. coli lacZ (beta-D-galactosidase) Genomic DNA E. coli neomycin resistance gene genomic DNA
Depositor|Developer Saito, Izumu |
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E. coli lacZ (beta-D-galactosidase) E. coli neomycin resistance gene
          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB01750.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
          Additional terms and conditions:
          Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。
          付加的提供条件：
          CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書 [Word]
          備考 【追加提供依頼書】受入れ確認書(書式J)
          組換え体提供にかかる書式が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB01750 AxCALNLNZ Under QC test. Please contact us. Virus, lysate of infected cell culture

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Original reference

          original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Arranged vector (English text)
          Featured content Expression regulation (English text)

          QC test results

          References

          Original, user report and related articles

          original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
          reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837.
          user_report Salah, M., An in vitro system to characterize prostate cancer progression identified signaling required for self-renewal. Mol. Carcinog. 55 (12): 1974-1989 (2016). PMID 26621780.
          user_report Nodagashira T., Efficient Gene Transduction in HER2-Expressing Cells by Two Recombinant Adenovirus Vectors with HER2 Promotor and Cre/loxP System. Hirosaki Med. J., 61: 26-34 (2010).
          user_report Gao Z., Delection of the PDGFR-beta Gene Affects Key Fibroblast Functions Important for Wound Healing J. Biol. Chem., 280, 9375-9389 (2005). PMID 15590688.
          user_report Nagayama Y., Targeting the replication of adenovirus to p53-defective thyroid carcinoma with a p53-regulated Cre/loxP system. Cancer Gene Ther., 8, 36-44 (2001). PMID 11219492.
          user_report Yokoo T., Inflamed glomeruli-specific gene activation that uses recombinant adenovirus with the Cre/loxP system. J. Am. Soc. Nephrol., 12, 2330-2337 (2001). PMID 11675409.
          user_report Araki T., Shp-2 specifically regulates several tyrosine-phosphorylated proteins in brain-derived neurotrophic factor signaling in cultured cerebral cortical neurons. Neurochem., 74, 659-668 (2000). PMID 10646517.

          2022.10.11

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