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AxCANLacZ

Recombinant adenovirus expressing beta-galactosidase

Catalog number RDB01749
Resource name AxCANLacZ
Clone info. Recombinant adenovirus expressing beta-galactosidase tagged with a nuclear localization signal under the control of CAG promoter.
Present lot is RDB03211.
Vector backbone pAxcw (Adenovirus)
Size of vector backbone -
Growth remarks 5 percent FCS is fine.
Gene/insert name E. coli lacZ (beta-D-galactosidase) Genomic DNA
Depositor|Developer Saito, Izumu |
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External Database
E. coli lacZ (beta-D-galactosidase)

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書 [Word]
          備考 【追加提供依頼書】受入れ確認書(書式J)
          組換え体提供にかかる書式が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB01749 AxCANLacZ Under QC test. Please contact us. Virus, lysate of infected cell culture

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
          Ordering Information [in Japanese] [in English]

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB01749.gif

          Original reference

          original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information


          References

          Original, user report and related articles

          original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
          reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837.
          user report Morimoto, H., ROS amplification drives mouse spermatogonial stem cell self-renewal. Life Sci. Alliance 2 (2). pii: e201900374 (2019). PMID 30940732.
          user report Kanatsu-Shinohara, M., Myc/Mycn-mediated glycolysis enhances mouse spermatogonial stem cell self-renewal. Genes Dev. 30 (23): 2637-2648 (2016). PMID 28007786.
          user report Kanatsu-Shinohara, M., Skp1-Cullin-F-box (SCF)-type ubiquitin ligase FBXW7 negatively regulates spermatogonial stem cell self-renewal. Proc. Natl. Acad. Sci. U.S.A., 111 (24): 8826-8831 (2014). PMID 24879440.
          user report Takehashi, M., Adenovirus-mediated gene delivery into mouse spermatogonial stem cells. Proc. Natl. Acad. Sci. U. S. A., 104 (8): 2596-2601 (2007). PMID 17299052.
          user report Dali-Youcef N., Adipose tissue-specific inactivation of the retinoblastoma protein protects against diabesity because of increased energy expenditure. Proc. Natl. Acad. Sci. U S A., 104, 10703-10708 (2007). PMID 17556545.
          user report Suzuki E., A simple method for the simultaneous detection of E1A and E1B in adenovirus stocks. Oncol. Rep., 11, 173-178 (2004). PMID 14654922.
          user report Naiki T., Analysis of gene expression profile induced by hepatocyte nuclear factor 4alpha in hepatoma cells using an oligonucleotide microarray. J. Biol. Chem., 277, 14011-14019(2002). PMID 11834723.
          user report Ugai H., Stability of a Recombinant Adenoviral Vector: Optimization of Conditions for Storage, Transport and Delivery Jpn. J. Cancer Res., 93, 598-603 (2002). PMID 12036457.
          user report Mishima A., Over-expression of PAR-3 suppresses contact-mediated inhibition of cell migration in MDCK cells. Genes Cells., 7, 581-596 (2002). PMID 12059961.
          user report Suzuki A., aPKC kinase activity is required for the asymmetric differentiation of the premature junctional complex during epithelial cell polarization. J. Cell Sci., 115, 3565-3573 (2002). PMID 12186943.
          user report Suzuki A., Atypical protein kinase C is involved in the evolutionarily conserved par protein complex and plays a critical role in establishing epithelia-specific junctional structures. J. Cell Biol., 152, 1183-1196 (2001). PMID 11257119.
          user report Yamanaka T., PAR-6 regulates aPKC activity in a novel way and mediates cell-cell contact-induced formation of the epithelial junctional complex. Genes Cells, 6, 721-731 (2001). PMID 11532031.
          user report Yokoo T., Inflamed glomeruli-specific gene activation that uses recombinant adenovirus with the Cre/loxP system. J. Am. Soc. Nephrol., 12, 2330-2337 (2001). PMID 11675409.
          user report Hirashima Y., Transfection of the plasma-type platelet-activating factor acetylhydrolase gene attenuates glutamate-induced apoptosis in cultured rat cortical neurons. Brain Res., 885, 128-132 (2000). PMID 11121539.

          2022.02.09

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