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AxCANLacZ (#RDB01749)

Recombinant adenovirus expressing beta-galactosidase

Clone info. Recombinant adenovirus expressing beta-galactosidase tagged with a nuclear localization signal under the control of CAG promoter.
Vector backbone pAxcw (RDB00917) (Adenovirus)
Size of vector backbone -
Growth remarks 5 percent FCS is fine.
Gene/insert name E. coli lacZ (beta-D-galactosidase) Genomic DNA
Depositor|Developer Saito, Izumu |
 
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Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考 【追加提供依頼書】受入れ確認書(書式J)
組換え体提供にかかる書式が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01749 AxCANLacZ Virus, lysate of infected cell culture

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The AxCANLacZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01749).

Reference section:

Kanegae, Y., Lee, G., Sato, Y., Tanaka, M., Nakai, M., Sakaki, T., Sugano, S., Saito, I., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB03211_03E9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CAG_R1 (Pr0584)
Region: CAG pro,SV40 NLS,lacZ 5'
Sequence file: RDB03211_03E9e.seq check
>01749_03211_03E9_#3_CAG_R1_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNCNAGCGGG CGCGGGGCGA ANCGGTGCGG CGCCGGCAGG
   61 AAGGAAANGG GCGGGGAGGG CCTTCGTGCG TCGCCGCGCC GCCGTCCCCT TCTCCCTCTC
  121 CAGCCTCGGG GCTGTCCGCG GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG
  181 TTCGGCTTCT GGCGTGTGAC CGGCGGCTCT AGAGCCTCTG CTAACCATGT TCATGCCTTC
  241 TTCTTTTTCC TACAGCTCCT GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC
  301 AAAGAATTGA TTTATCGATT TGCAGACCGT GCATCATGAG CGGCCCTCCA AAAAAGAAGA
  361 GAAAGGTAGA AGACCCGGGC GGCCGCGGGG ATCCCGTCGT TTTACAACGT CGTGACTGGG
  421 AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC
  481 GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG
  541 AATGGCGCTT TGCCTGGTTT CCGGCACCAG AAGCGGTGCC GGAAAGCTGG CTGGAGTGCG
  601 ATCTTCCTGA GGCCGATACT GTCGTCGTCC CCTCAAACTG GCANATGCAC GGTTACNATG
  661 CGCCCATCTA CACCAACGTG ACCTATCCCA TTACGGTCAA TCCGCCGTTT GNTNNCACGG
  721 ANAATCCGAC NGGTTGNTAC TCGCTCACAT TTNATGNTGA TGAAANCTGG CTACAGGNNN
  781 NNCNGANGCN AATTATTTTT GANGGCGTTN ACTCNGCGTT TCNTCTGTGG NGNNANNGGC
  841 GCTNGGNCNG NTNCNGNCAG GNCAGNCNNN NNNCNNCTGA ANTNNANCNG NNCGNNNTTT
901 TTACNCNNCN GNANAAAANN NNCNNCNNNG NNNNNNNNNN
//
Primer: pCA-bgter (Pr0066)
Region: lacZ 3'
Sequence file: RDB03211_03E9f.seq check
>01749_03211_03E9_#3_pCA-bgter_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNT TTGNNNNNNG GNNNNNNNNC NNNNNGGTAT TTGTGAGCCA
   61 GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA GGAGTGAATT
  121 GATTATCGAT TCTAGACTAG TTTAATTAAT TTTCGACGGA TCCCCCCTGC CCGGTTATTA
  181 TTATTTTTGA CACCAGACCA ACTGGTAATG GTAGCGACCG GCGCTCAGCT GGAATTCCGC
  241 CGATACTGAC GGGCTCCAGG AGTCGTCGCC ACCAATCCCC ATATGGAAAC CGTCGATATT
  301 CAGCCATGTG CCTTCTTCCG CGTGCAGCAG ATGGCGATGG CTGGTTTCCA TCAGTTGCTG
  361 TTGACTGTAG CGGCTGATGT TGAACTGGAA GTCGCCGCGC CACTGGTGTG GGCCATAATT
  421 CAATTCGCGC GTCCCGCAGC GCAGACCGTT TTCGCTCGGG AAGACGTACG GGGTATACAT
  481 GTCTGACAAT GGCAGATCCC AGCGGTCAAA ACAGGCGGCA GTAAGGCGGT CGGGATAGTT
  541 TTCTTGCGGC CCTAATCCGA NCCAGTTTAC CCGCTCTGCT ACCTGCGCCA GCTGGCAGTT
  601 CAGGNCAATC CGCGCCGGAN GCGGTGTATC GCTCGNCACT TCAACATCAA CGGTAATCNC
  661 CATTTGANCA CTACCATCAA TCCGGTANGN TTTCCGGNTG ANAAATAANN NTTTCCCCTG
  721 ATGCTGCCAC GCGTGANCNG NCNTNATCNN CACCGCATCA NCAAGTGTAT CTGNNNTGCN
  781 NTGNNACNAC GCTGCTTCNG CCTGGTANNG GNNCCGCCNN CNTTNNNNCG NNCGANCCNN
  841 GCGTTANGGT CNNNNNNGGG TNNCNNNNNT TANNNCNNNN GNCNNTATCC NNCNNNGCNN
901 GGNNNANNTN NNCNCNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]
reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837. [link to RRC of NBRP]
user_report Miyazaki, T., Glutamine protects mouse spermatogonial stem cells against NOX1-derived ROS for sustaining self-renewal division in vitro. Development 150 (20): dev201157 (2023). PMID 36897562. [link to RRC of NBRP]
user_report Kanatsu-Shinohara, M., Adenovirus-mediated gene delivery restores fertility in congenitally infertile female mice. J. Reprod. Dev. 68(6): 369-376 (2022). PMID 36223953. [link to RRC of NBRP]
user_report Morimoto, H., ROS amplification drives mouse spermatogonial stem cell self-renewal. Life Sci. Alliance 2 (2). pii: e201900374 (2019). PMID 30940732. [link to RRC of NBRP]
user_report Kanatsu-Shinohara, M., Myc/Mycn-mediated glycolysis enhances mouse spermatogonial stem cell self-renewal. Genes Dev. 30 (23): 2637-2648 (2016). PMID 28007786. [link to RRC of NBRP]
user_report Kanatsu-Shinohara, M., Skp1-Cullin-F-box (SCF)-type ubiquitin ligase FBXW7 negatively regulates spermatogonial stem cell self-renewal. Proc. Natl. Acad. Sci. U.S.A., 111 (24): 8826-8831 (2014). PMID 24879440. [link to RRC of NBRP]
user_report Takehashi, M., Adenovirus-mediated gene delivery into mouse spermatogonial stem cells. Proc. Natl. Acad. Sci. U. S. A., 104 (8): 2596-2601 (2007). PMID 17299052. [link to RRC of NBRP]
user_report Dali-Youcef N., Adipose tissue-specific inactivation of the retinoblastoma protein protects against diabesity because of increased energy expenditure. Proc. Natl. Acad. Sci. U S A., 104, 10703-10708 (2007). PMID 17556545. [link to RRC of NBRP]
user_report Suzuki E., A simple method for the simultaneous detection of E1A and E1B in adenovirus stocks. Oncol. Rep., 11, 173-178 (2004). PMID 14654922. [link to RRC of NBRP]
user_report Naiki T., Analysis of gene expression profile induced by hepatocyte nuclear factor 4alpha in hepatoma cells using an oligonucleotide microarray. J. Biol. Chem., 277, 14011-14019(2002). PMID 11834723. [link to RRC of NBRP]
user_report Ugai H., Stability of a Recombinant Adenoviral Vector: Optimization of Conditions for Storage, Transport and Delivery Jpn. J. Cancer Res., 93, 598-603 (2002). PMID 12036457. [link to RRC of NBRP]
user_report Mishima A., Over-expression of PAR-3 suppresses contact-mediated inhibition of cell migration in MDCK cells. Genes Cells., 7, 581-596 (2002). PMID 12059961. [link to RRC of NBRP]
user_report Suzuki A., aPKC kinase activity is required for the asymmetric differentiation of the premature junctional complex during epithelial cell polarization. J. Cell Sci., 115, 3565-3573 (2002). PMID 12186943. [link to RRC of NBRP]
user_report Suzuki A., Atypical protein kinase C is involved in the evolutionarily conserved par protein complex and plays a critical role in establishing epithelia-specific junctional structures. J. Cell Biol., 152, 1183-1196 (2001). PMID 11257119. [link to RRC of NBRP]
user_report Yamanaka T., PAR-6 regulates aPKC activity in a novel way and mediates cell-cell contact-induced formation of the epithelial junctional complex. Genes Cells, 6, 721-731 (2001). PMID 11532031. [link to RRC of NBRP]
user_report Yokoo T., Inflamed glomeruli-specific gene activation that uses recombinant adenovirus with the Cre/loxP system. J. Am. Soc. Nephrol., 12, 2330-2337 (2001). PMID 11675409. [link to RRC of NBRP]
user_report Hirashima Y., Transfection of the plasma-type platelet-activating factor acetylhydrolase gene attenuates glutamate-induced apoptosis in cultured rat cortical neurons. Brain Res., 885, 128-132 (2000). PMID 11121539. [link to RRC of NBRP]