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AxCANCre

Recombinant adenovirus expressing recombinase Cre with nuclear localization signal

Catalog number RDB01748
Resource name AxCANCre
Alternative name
Clone info. Recombinant adenovirus expressing the recombinase Cre tagged with a nuclear localization signal under the control of CAG promoter.
Vector backbone pAxcw (Adenovirus)
Size of vector backbone -
Growth remarks 5 percent FCS is fine.
Gene/insert name P1 phage Cre recombinase cDNA
Depositor|Developer Saito, Izumu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01748 AxCANCre Virus, lysate of infected cell culture

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Remarks, protocol and/or map (gif) RDB01748.gif

Original reference

original Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332.
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Cre-loxP and FLP-FRT System (English text)
Featured content Dnaconda's recommendation EXR0066e (English text)
Featured content Dnaconda's recommendation EXR0066j (Japanese text)
Featured content Control Vectors (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB01748_020904p2-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F
Sequence file: RDB01748_020904a.seq check
>01748_01748_A6C4_2_pCAG-F_B01_02_ABI08.ab1
    1 GCTGGGGGGG AAAGCTGCCT TCGGGGGGGA GGGGCAGGGC GGGGTTCGGC TTCTGGCGTG
   61 TGACCGGCGG CTCTAGAGCC TCTGCTAACC ATGTTCATGC CTTCTTCTTT TTCCTACAGC
  121 TCCTGGGCAA CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTGATTTATC
  181 GATTTGCAGA CCGTGCATCA TGAGCGGCCC TCCAAAAAAG AAGAGAAAGG TAGAAGACCC
  241 GGGCGGCCGC ATGTCCAATT TACTGACCGT ACACCAAAAT TTGCCTGCAT TACCGGTCGA
  301 TGCAACGAGT GATGAGGTTC GCAAGAACCT GATGGACATG TTCAGGGATC GCCAGGCGTT
  361 TTCTGAGCAT ACCTGGAAAA TGCTTCTGTC CGTTTGCCGG TCGTGGGCGG CATGGTGCAA
  421 GTTGAATAAC CGGAAATGGT TTCCCGCAGA ACCTGAAGAT GTTCGCGATT ATCTTCTATA
  481 TCTTCAGGCG CGCGGTCTGG CAGTAAAAAC TATCCAGCAA CATTTGGGCC AGCTAAACAT
  541 GCTTCATCGT CGGTCCGGGC TGCCACGACC AAGTGACAGC AATGCTGTTT CACTGGTTAT
  601 GCGGCGGATC CGAAAAGAAA ACGTTGATGC CGGTGAACGT GCAAAACAGG CTCTAGCGTT
  661 CGAACGCACT GATTTCGACC AGGTTCGTTC ACTCATGGAA AATAGCGATC GCTGCCAGGA
  721 TATACGTAAT CTGGCATTTC TGGGGATTGC TTATAACACC CTGTTACGTA TAGCCGAAAT
  781 TGCCAGGATC AGGGTTAAAG ATATCTCACG TACTGACGGT GGGAGAATGT TAATCCATAT
  841 TGGCAGAACG AAAACGCTGG TTAGCACCGC AGTGTAGAGA AGGCACTTAG CCTGGGGGGT
  901 AACTAAACTG GTCGAGCGAT GGATTTCCGT CTCTGGTGTA GCTGATGATC CGAATAACTA
  961 CCCTGTTTTG CTGATCAGAA AAAAATGGGT GTTTGCCCGC GCCATCTGCC ACAGCCCAGG
 1021 CTATCCACTT CGCGCCCCTG GGAAAGGATT TTGGAGGCCA ACTCATCCGA ATTGGATTAC
1081 GCGCTAGAAT GACTCTGGTT CAGGAGATAC CTGTGGTC
//
Primer: pAxcwit2_Rv
Sequence file: RDB01748_020904b.seq check
>01748_01748_A6C4-7_3_pAxcwit2_Rv_B02_05_ABI24.ab1
    1 TTGGGCACTG CATAGAAGAG TGAATCTGAA TAATTTTGTG TTACTCATAG CGCGTAATAT
   61 TTGTCTAGGG CCGCGGGGAC TTTGACCGTT TACGTGGAGA CTCGCCCAGG TGTTTTTCTC
  121 AGGTGTTTTC CGCGTTCCGG GTCAAAGTTG GCGTTTTATT ATTATAGTCA GCATCGTTTA
  181 AACAGCTTGG GCCCTCGAGG GGTCGAGGGA TCTCCATAAG AGAAGAGGGA CAGCTATGAC
  241 TGGGAGTAGT CAGGAGAGGA GGAAAAATCT GGCTAGTAAA ACATGTAAGG AAAATTTTAG
  301 GGATGTTAAA GAAAAAAATA ACACAAAACA AAATATAAAA AAAATCTAAC CTCAAGTCAA
  361 GGCTTTTCTA TGGAATAAGG AATGGACAGC AGGGGGCTGT TTCATATACT GATGACCTCT
  421 TTATAGCCAA CCTTTGTTCA TGGCAGCCAG CATATGGGCA TATGTTGCCA AACTCTAAAC
  481 CAAATACTCA TTCTGATGTT TTAAATGATT TGCCCTCCCA TATGTCCTTC CGAGTGAGAG
  541 ACACAAAAAA TTCCAACACA CTATTGCAAT GAAAATAAAT TTCCTTTATT AGCCAGAAGT
  601 CAGATGCTCA AGGGGCTTCA TGATGTCCCC ATAATTTTTG GCAGAGGGAA AAAGATCTCA
  661 GTGGTATTTG TGAGCCAGGG CATTGGCCAC ACCAGCCACC ACCTTCTGAT AGGCAGCCTG
  721 CACCTGAGGA GTGAATTGAT TATCGATTCT AGACTAGTTT AATTAATTTC TAGAGCGCTT
  781 AATGGCTAAT CGCCATCTTC CAGCAGGCGC ACCATTGCCC CTGTTTCACT ATCCAGGTTA
  841 CGGATATAGT TCATGACAAT ATTTACATTG GTCCAGCCAC CAGCTTGCAT GATCTCCGGT
  901 ATTGAAACTC CAGCGCGGGC CATATCTCGC GCGGCTCCGA CACGGGCACT GTGTCCAGAC
  961 CAGGCCAGGT ATCTCTGACC AGAGTCATCC TTAGCGCCGT AAATCAATCG ATGAGTTGCT
 1021 TCAAAAATCC CTTCCAGGCG CGAGTTTGAT AGCTGGCTGG GTGGCAGATG GCGCGGCAAC
 1081 ACCATTTTTT CCTGAACTCG CAAACAGTTA GGTTATTCGG ATCATCCAGG CCTTAACAAC
1141 CCAGGACAG
//
Primer: pAxCA_R1
Sequence file: RDB01748_020904c.seq check
>01748_01748_A6C4_2_pAxCA_R1_A01_01_ABI08.ab1
    1 TTTTGGAAAG CCATTGGCCA CACCAGCCAC CACCTTCTGA TAGGCAGCCT GCACCTGAGG
   61 AGTGAATTGA TTATCGATTC TAGACTAGTT TAATTAATTT CTAGAGCGCT TAATGGCTAA
  121 TCGCCATCTT CCAGCAGGCG CACCATTGCC CCTGTTTCAC TATCCAGGTT ACGGATATAG
  181 TTCATGACAA TATTTACATT GGTCCAGCCA CCAGCTTGCA TGATCTCCGG TATTGAAACT
  241 CCAGCGCGGG CCATATCTCG CGCGGCTCCG ACACGGGCAC TGTGTCCAGA CCAGGCCAGG
  301 TATCTCTGAC CAGAGTCATC CTTAGCGCCG TAAATCAATC GATGAGTTGC TTCAAAAATC
  361 CCTTCCAGGG CGCGAGTTGA TAGCTGGCTG GTGGCAGATG GCGCGGCAAC ACCATTTTTT
  421 CTGACCCGGC AAAACAGGTA GTTATTCGGA TCATCAGCTA CACCAGAGAC GGAAATCCAT
  481 CGCTCGACCA GTTTAGTTAC CCCCAGGCTA AGTGCCTTCT CTACACCTGC GGTGCTAACC
  541 AGCGTTTTCG TTCTGCCAAT ATGGATTAAC ATTCTCCCAC CGTCAGTACG TGAGATATCT
  601 TTAACCCTGA TCCTGGCAAT TTCGGCTATA CGTAACAGGG TGTTATAAGC AATCCCCAGA
  661 AATGCCAGAT TACGTATATC CTGGCAGCGA TCGCTATTTT CCATGAGTGA ACGAACCTGG
  721 TCGAAATCAG TGCGTTCGAA CGCTAGAGCC TGTTTTGCAC GTTCACCGGC ATCAACGTTT
  781 TCTTTTCGGA TCCGCCGCAT AACCAGTGAA ACAGCATTGC TGTCACTTGG TCGTGGCAGC
  841 CCGGACCGAC GATGAAGCAT GTTTAGCTGG CCCAAATGTT GCTGGGATAG TTTTTTACTG
  901 CCAGACCGCG CGCCTGAAGA TATAGGAAGA TAATCGCGAA CATCTTTCAG GTTCTGCGGG
  961 AAACCCATTT CCCGGTTATT CAACTTTGCA CCATGCCGCC ACGACCGGGC AAAACGGACA
 1021 GAGGCATTTT CCAGTAATGC TTCAGAAACC GCCTTGGCCG AATCCCTGGA CCATGGTCAT
1081 TCAGTCTGCG ACCTCATCAA CTCGATTG
//
Primer: CMV_en_R
Sequence file: RDB01748_020904d.seq check
>01748_01748_A6C4-7_3_CMV_en_R_C01_03_ABI08.ab1
    1 TCGTGGAATT ACTATTAATA ACTAGTCAAT AATCAATGTC GACATCGGTT TAAACGATTG
   61 TCGACTCGCG ACGATGGATC TGGAAGGTGC TGAGGTACGA TGAGACCCGC ACCAGGTGCA
  121 GACCCTGCGA GTGTGGCGGT AAACATATTA GGAACCAGCC TGTGATGCTG GATGTGACCG
  181 AGGAGCTGAG GCCCGATCAC TTGGTGCTGG CCTGCACCCG CGCTGAGTTT GGCTCTAGCG
  241 ATGAAGATAC AGATTGAGGT ACTGAAATGT GTGGGCGTGG CTTAAGGGTG GGAAAGAATA
  301 TATAAGGTGG GGGTCTTATG TAGTTTTGTA TCTGTTTTGC AGCAGCCGCC GCCGCCATGA
  361 GCACCAACTC GTTTGATGGA AGCATTGTGA GCTCATATTT GACAACGCGC ATGCCCCCAT
  421 GGGCCGGGGT GCGTCAGAAT GTGATGGGCT CCAGCATTGA TGGTCGCCCC GTCCTGCCCG
  481 CAAACTCTAC TACCTTGACC TACGAGACCG TGTCTGGAAC GCCGTTGGAG ACTGCAGCCT
  541 CCGCCGCCGC TTCAGCCGCT GCAGCCACCG CCCGCGGGAT TGTGACTGAC TTTGCTTTCC
  601 TGAGCCCGCT TGCAAGCAGT GCAGCTTCCC GTTCATCCGC CCGCGATGAC AAGTTGACGG
  661 CTCTTTTGGC ACAATTGGAT TCTTTGACCC GGGAACTTAA TGTCGTTTCT CAGCAGCTGT
  721 TGGATCTGCG CCAGCAGGTT TCTGCCCTGA AGGCTTCCTC CCCTCCCAAT GCGGTTTAAA
  781 ACATAAATAA AAAACCAGAC TCTGTTTGGA TTTGGATCAA GCAAGTGTCT TGCTGTCTTT
  841 ATTTAGGGGT TTTGCGCGCG CGGTAGGCCC GGGGACCAGC GGTCTCGGGT CGTTGAGGGT
  901 CCTGTGTATT TTTTTCCAGG ACGTGGTAAA GGTGACTCTG GGATGTTCAG ATACATGGGG
  961 CATAAGCCCG GTCTCTGGGG TGGGAGGGTA GCACCACTGC AGAGCTTTCA TGCTGCGGGG
 1021 TGTTGTTGTA GATGATTCCA GTCGTAGCAA GAAGCGGCTG GCCGTTGCAT GGCTAAAAAT
1081 TGTTCGTTTC AGTAGCCAAG C
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332.
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
reference Hosoya, M., Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss. Cell Rep. 18 (1): 68-81 (2017). PMID 28052261.
reference Danno, S., PKN2 is essential for mouse embryonic development and proliferation of mouse fibroblasts. Genes Cells 22 (2): 220-236 (2017) PMID 28102564.
reference Sato, T., JSAP1/JIP3 and JLP regulate kinesin-1-dependent axonal transport to prevent neuronal degeneration. Cell Death Differ. 22 (8): 1260-1274 (2015). PMID 25571974.
reference Tuvshintugs, B., JSAP1 and JLP are required for ARF6 localization to the midbody in cytokinesis. Genes Cells 19 (9): 692-703 (2014). PMID 25130574.
reference Fukamachi, K., A novel reporter rat strain that expresses LacZ upon Cre-mediated recombination. Genesis, 51 (4): 268-274 (2013). PMID 23349063.
reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-199 (1991). PMID 1660837.
user report Morimoto, H., ROS amplification drives mouse spermatogonial stem cell self-renewal. Life Sci. Alliance 2 (2). pii: e201900374 (2019). PMID 30940732.
user report Morita, M., PKM1 Confers Metabolic Advantages and Promotes Cell-Autonomous Tumor Cell Growth. Cancer Cell 33 (3): 355-367.e7 (2018). PMID 29533781.
user report Watanabe, S., In Vivo Genetic Manipulation of Spermatogonial Stem Cells and Their Microenvironment by Adeno-Associated Viruses. Stem Cell Reports 10 (5): 1551-1564 (2018). PMID 29628393.
user report Salah, M., An in vitro system to characterize prostate cancer progression identified signaling required for self-renewal. Mol. Carcinog. 55 (12): 1974-1989 (2016). PMID 26621780.
user report Chanmee, T., Hyaluronan Production Regulates Metabolic and Cancer Stem-like Properties of Breast Cancer Cells via Hexosamine Biosynthetic Pathway-coupled HIF-1 Signaling. J. Biol. Chem. 291 (46): 24105-24120 (2016). PMID 27758869.
user report Kanatsu-Shinohara, M., Myc/Mycn-mediated glycolysis enhances mouse spermatogonial stem cell self-renewal. Genes Dev. 30 (23): 2637-2648 (2016). PMID 28007786.
user report Kurihara, C., An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes. Biotechnol. Rep. (Amst.) 12: 26-32 (2016). PMID 28352551.
user report Tanaka, T., The CDKN1B-RB1-E2F1 pathway protects mouse spermatogonial stem cells from genomic damage. J. Reprod. Dev. 61 (4): 305-316 (2015). PMID 25959802.
user report Kanatsu-Shinohara, M., Skp1-Cullin-F-box (SCF)-type ubiquitin ligase FBXW7 negatively regulates spermatogonial stem cell self-renewal. Proc. Natl. Acad. Sci. U.S.A., 111 (24): 8826-8831 (2014). PMID 24879440.
user report Matsushita, Y., Microglia activation precedes the anti-opioid BDNF and NMDA receptor mechanisms underlying morphine analgesic tolerance. Curr. Pharm. Des. 19 (42): 7355-7361 (2013). PMID 23448475.
user report Egawa, N., The E1 protein of human papillomavirus type 16 is dispensable for maintenance replication of the viral genome. J. Virol., 86 (6): 3276-3283 (2012). PMID 22238312.
user report Kumadaki, S., Inhibition of Ubiquitin ligase F-box and WD repeat domain-containing 7(alpha) (Fbw7(alpha}) causes hepatosteatosis through the Kruppel-like factor 5 (KLF5)/PPAR(gamma)2 pathway, but not SREBP-1c in mice. J. Biol. Chem., 286 (47): 40835-40846 (2011). PMID 21911492.
user report Nodagashira T., Efficient Gene Transduction in HER2-Expressing Cells by Two Recombinant Adenovirus Vectors with HER2 Promotor and Cre/loxP System. Hirosaki Med. J., 61: 26-34 (2010).
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user report Gao Z., Delection of the PDGFR-beta Gene Affects Key Fibroblast Functions Important for Wound Healing J. Biol. Chem., 280, 9375-9389 (2005). PMID 15590688.
user report Yanagihara M., Ribozymes targeting serine/threonine kinase Akt1 sensitize cells to anticancer drugs. Cancer Sci., 96, 620-6 (2005). PMID 16128748.
user report Tanaka S., Mitochondrial impairment induced by poly(ADP-ribose) polymerase-1 activation in cortical neurons after oxygen and glucose deprivation. J. Neurochem., 95, 179-190 (2005). PMID 16181422.
user report Nishitai G., Stress induces mitochondria-mediated apoptosis independent of SAPK/JNK activation in embryonic stem cells. J. Biol. Chem., 279, 1621-1626 (2004). PMID 14585831.
user report Mizukami H., Separate control of Rep and Cap expression using mutant and wild-type loxP sequences and improved packaging system for adeno-associated virus vector production. Mol. Biotechnol., 27, 7-14 (2004). PMID 15122043.
user report Matsuura K., Cardiomyocytes fuse with surrounding noncardiomyocytes and reenter the cell cycle. J. Cell Biol., 167, 351-363 (2004). PMID 15492039.
user report Aihara M., Angiogenic endothelium-specific nestin expression is enhanced by the first intron of the nestin gene. Lab. Invest., 84, 1581-1592 (2004). PMID 15502861.
user report Suzuki K., Adenovirus-mediated gene transfer of interferon alpha improves dimethylnitrosamine-induced liver cirrhosis in rat model. Gene Ther., 10, 765-773 (2003). PMID 12704415.
user report Furuyama T., Forkhead transcription factor FOXO1 (FKHR)-dependent induction of PDK4 gene expression in skeletal muscle during energy deprivation. Biochem. J., 375, 365-371 (2003). PMID 12820900.
user report Nishioka K., Immortalization of bone marrow-derived human mesenchymal stem cells by removable simian virus 40T antigen gene: analysis of the ability to support expansion of cord blood hematopoietic progenitor cells. Int. J. Oncol., 23, 925-32 (2003). PMID 12963970.
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user report Iida A., Cytoplasmic RNA vector derived from nontransmissible Sendai virus. Production and use. Methods Mol. Med., 69, 361-370 (2002). PMID 11987789.
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user report Fukazawa T., Accelerated degradation of cellular FLIP protein through the ubiquitin-proteasome pathway in p53-mediated apoptosis of human cancer cells. Oncogene, 20, 5225-5231 (2001). PMID 11526513.
user report Kobayashi N., Cre/loxP-based reversible immortalization of human hepatocytes. Cell Transplant., 10, 383-386 (2001). PMID 11549058.
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2021.07.10

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