Resource data sheet


Recombinant adenovirus expressing recombinase Cre with nuclear localization signal

Catalog number RDB01748
Resource name AxCANCre
Alternative name
Clone info. Recombinant adenovirus expressing the recombinase Cre tagged with a nuclear localization signal under the control of CAG promoter.
Vector backbone pAxcw (Adenovirus)
Size of vector backbone -
Gene/insert name P1 phage Cre recombinase cDNA
Depositor Saito, Izumu |
Other clones in our bank P1 phage cre |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
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Material Transfer Agreement (MTA) [Word]
Remarks ((Additional form)) CAG(FormE)
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RDB01748 AxCANCre Please contact us. Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01748.gif from Depositor

Featured content

Featured content Cre-loxP and FLP-FRT System (English text)
Featured content Dnaconda's recommendation EXR0066e (English text)
Featured content Dnaconda's recommendation EXR0066j (Japanese text)
Featured content Control Vectors (English text)


original Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332.
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

User reports and related articles (go to bottom)

Sequence information



original Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332.
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
reference Hosoya, M., Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss. Cell Rep. 18 (1): 68-81 (2017). PMID 28052261.
reference Danno, S., PKN2 is essential for mouse embryonic development and proliferation of mouse fibroblasts. Genes Cells 22 (2): 220-236 (2017) PMID 28102564.
reference Sato, T., JSAP1/JIP3 and JLP regulate kinesin-1-dependent axonal transport to prevent neuronal degeneration. Cell Death Differ. 22 (8): 1260-1274 (2015). PMID 25571974.
reference Tuvshintugs, B., JSAP1 and JLP are required for ARF6 localization to the midbody in cytokinesis. Genes Cells 19 (9): 692-703 (2014). PMID 25130574.
reference Fukamachi, K., A novel reporter rat strain that expresses LacZ upon Cre-mediated recombination. Genesis, 51 (4): 268-274 (2013). PMID 23349063.
reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-199 (1991). PMID 1660837.
user report Morita, M., PKM1 Confers Metabolic Advantages and Promotes Cell-Autonomous Tumor Cell Growth. Cancer Cell 33 (3): 355-367.e7 (2018). PMID 29533781.
user report Watanabe, S., In Vivo Genetic Manipulation of Spermatogonial Stem Cells and Their Microenvironment by Adeno-Associated Viruses. Stem Cell Reports 10 (5): 1551-1564 (2018). PMID 29628393.
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user report Chanmee, T., Hyaluronan Production Regulates Metabolic and Cancer Stem-like Properties of Breast Cancer Cells via Hexosamine Biosynthetic Pathway-coupled HIF-1 Signaling. J. Biol. Chem. 291 (46): 24105-24120 (2016). PMID 27758869.
user report Kanatsu-Shinohara, M., Myc/Mycn-mediated glycolysis enhances mouse spermatogonial stem cell self-renewal. Genes Dev. 30 (23): 2637-2648 (2016). PMID 28007786.
user report Kurihara, C., An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes. Biotechnol. Rep. (Amst.) 12: 26-32 (2016). PMID 28352551.
user report Tanaka, T., The CDKN1B-RB1-E2F1 pathway protects mouse spermatogonial stem cells from genomic damage. J. Reprod. Dev. 61 (4): 305-316 (2015). PMID 25959802.
user report Kanatsu-Shinohara, M., Skp1-Cullin-F-box (SCF)-type ubiquitin ligase FBXW7 negatively regulates spermatogonial stem cell self-renewal. Proc. Natl. Acad. Sci. U.S.A., 111 (24): 8826-8831 (2014). PMID 24879440.
user report Matsushita, Y., Microglia activation precedes the anti-opioid BDNF and NMDA receptor mechanisms underlying morphine analgesic tolerance. Curr. Pharm. Des. 19 (42): 7355-7361 (2013). PMID 23448475.
user report Egawa, N., The E1 protein of human papillomavirus type 16 is dispensable for maintenance replication of the viral genome. J. Virol., 86 (6): 3276-3283 (2012). PMID 22238312.
user report Kumadaki, S., Inhibition of Ubiquitin ligase F-box and WD repeat domain-containing 7(alpha) (Fbw7(alpha}) causes hepatosteatosis through the Kruppel-like factor 5 (KLF5)/PPAR(gamma)2 pathway, but not SREBP-1c in mice. J. Biol. Chem., 286 (47): 40835-40846 (2011). PMID 21911492.
user report Nodagashira T., Efficient Gene Transduction in HER2-Expressing Cells by Two Recombinant Adenovirus Vectors with HER2 Promotor and Cre/loxP System. Hirosaki Med. J., 61: 26-34 (2010).
user report Takashima, S., Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects. Biol. Reprod., 81, 155-164 (2009). PMID 19228594.
user report Deurholt T., Novel immortalized human fetal liver cell line, cBAL111, has the potential to differentiate into functional hepatocytes. BMC Biotechnol., 9: 89 (2009). PMID 19845959.
user report Kanatsu-Shinohara, M., Homing of mouse spermatogonial stem cells to germline niche depends on beta1-integrin. Cell Stem Cell, 3 (5): 533-542 (2008). PMID 18983968.
user report Tomimatsu N., Ku70/80 modulates ATM and ATR signaling pathways in response to DNA double strand breaks. J. Biol. Chem., 282, 10138-10145 (2007). PMID 17272272.
user report Takehashi, M., Adenovirus-mediated gene delivery into mouse spermatogonial stem cells. Proc. Natl. Acad. Sci. U. S. A., 104 (8): 2596-2601 (2007). PMID 17299052.
user report Dali-Youcef N., Adipose tissue-specific inactivation of the retinoblastoma protein protects against diabesity because of increased energy expenditure. Proc. Natl. Acad. Sci. U S A., 104, 10703-10708 (2007). PMID 17556545.
user report Omoteyama K., Activation of connective tissue growth factor gene by the c-Maf and Lc-Maf transcription factors. Biochem. Biophys. Res. Commun., 339, 1089-1097 (2006). PMID 16343439.
user report Sakaki-Yumoto M., The murine homolog of SALL4, a causative gene in Okihiro syndrome, is essential for embryonic stem cell proliferation, and cooperates with Sall1 in anorectal, heart, brain and kidney development. Development, 133, 3005-3013 (2006). PMID 16790473.
user report Hoekstra R., Assessment of in vitro applicability of reversibly immortalized NKNT-3 cells and clonal derivatives. Cell Transplant., 15, 423-433 (2006). PMID 16970284.
user report Gao Z., Delection of the PDGFR-beta Gene Affects Key Fibroblast Functions Important for Wound Healing J. Biol. Chem., 280, 9375-9389 (2005). PMID 15590688.
user report Yanagihara M., Ribozymes targeting serine/threonine kinase Akt1 sensitize cells to anticancer drugs. Cancer Sci., 96, 620-6 (2005). PMID 16128748.
user report Tanaka S., Mitochondrial impairment induced by poly(ADP-ribose) polymerase-1 activation in cortical neurons after oxygen and glucose deprivation. J. Neurochem., 95, 179-190 (2005). PMID 16181422.
user report Nishitai G., Stress induces mitochondria-mediated apoptosis independent of SAPK/JNK activation in embryonic stem cells. J. Biol. Chem., 279, 1621-1626 (2004). PMID 14585831.
user report Mizukami H., Separate control of Rep and Cap expression using mutant and wild-type loxP sequences and improved packaging system for adeno-associated virus vector production. Mol. Biotechnol., 27, 7-14 (2004). PMID 15122043.
user report Matsuura K., Cardiomyocytes fuse with surrounding noncardiomyocytes and reenter the cell cycle. J. Cell Biol., 167, 351-363 (2004). PMID 15492039.
user report Aihara M., Angiogenic endothelium-specific nestin expression is enhanced by the first intron of the nestin gene. Lab. Invest., 84, 1581-1592 (2004). PMID 15502861.
user report Suzuki K., Adenovirus-mediated gene transfer of interferon alpha improves dimethylnitrosamine-induced liver cirrhosis in rat model. Gene Ther., 10, 765-773 (2003). PMID 12704415.
user report Furuyama T., Forkhead transcription factor FOXO1 (FKHR)-dependent induction of PDK4 gene expression in skeletal muscle during energy deprivation. Biochem. J., 375, 365-371 (2003). PMID 12820900.
user report Nishioka K., Immortalization of bone marrow-derived human mesenchymal stem cells by removable simian virus 40T antigen gene: analysis of the ability to support expansion of cord blood hematopoietic progenitor cells. Int. J. Oncol., 23, 925-32 (2003). PMID 12963970.
user report Noguchi H., Controlled expansion of human endothelial cell populations by Cre-loxP-based reversible immortalization. Hum. Gene Ther., 13, 321-334 (2002). PMID 11812287.
user report Iida A., Cytoplasmic RNA vector derived from nontransmissible Sendai virus. Production and use. Methods Mol. Med., 69, 361-370 (2002). PMID 11987789.
user report Kaartinen V., TGFbeta3-induced activation of RhoA/Rho-kinase pathway is necessary but not sufficient for epithelio-mesenchymal transdifferentiation: implications for palatogenesis. Int. J. Mol. Med., 9, 563-70 (2002). PMID 12011971.
user report Ohba Y., Requirement for C3G-dependent Rap1 activation for cell adhesion and embryogenesis. EMBO J., 20, 3333-3341 (2001). PMID 11432821.
user report Fukazawa T., Accelerated degradation of cellular FLIP protein through the ubiquitin-proteasome pathway in p53-mediated apoptosis of human cancer cells. Oncogene, 20, 5225-5231 (2001). PMID 11526513.
user report Kobayashi N., Cre/loxP-based reversible immortalization of human hepatocytes. Cell Transplant., 10, 383-386 (2001). PMID 11549058.
user report Yokoo T., Inflamed glomeruli-specific gene activation that uses recombinant adenovirus with the Cre/loxP system. J. Am. Soc. Nephrol., 12, 2330-2337 (2001). PMID 11675409.
user report Kaartinen V., Removal of the floxed neo gene from a conditional knockout allele by the adenoviral Cre recombinase in vivo. Genesis., 31, 126-129 (2001). PMID 11747203.
user report Araki T., Shp-2 specifically regulates several tyrosine-phosphorylated proteins in brain-derived neurotrophic factor signaling in cultured cerebral cortical neurons. Neurochem., 74, 659-668 (2000). PMID 10646517.
user report Kobayashi N., Prevention of acute liver failure in rats with reversibly immortalized human hepatocytes. Science, 287, 1258-1262 (2000). PMID 10678831.