Resource data sheet
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Ad5-dlX (#RDB01747)

E3-deleted adenovirus type 5

Alternative name E3-deleted adenovirus type 5
Clone info. Parent virus. E3-deleted adenovirus type5. A sourse of the DNA-TPC used in COS-TPC method.
Vector backbone Adenovirus
Size of vector backbone -
Growth remarks 5 percent FCS is fine.
Gene/insert name AdV_5 Adenovirus type 5
Depositor|Developer Saito, Izumu |
 
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Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Saito, I., J. Virol., 54, 711-719(1985)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Saito, I., J. Virol., 54, 711-719(1985))。
備考 【追加提供依頼書】受入れ確認書(書式J)
組換え体提供にかかる書式が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB01747 Ad5-dlX Under QC test. Please contact us. Virus, lysate of infected cell culture

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The Ad5-dlX was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01747).

Reference section:

Miyake, S., Makimura, M., Kanegae, Y., Harada, S., Sato, Y., Takamori, K., Tokuda, C., Saito, I., Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. USA, 93, 1320-1324 (1996). PMID 8577762. [link to RRC of NBRP]
Saito, I., Oya, Y., Yamamoto, K., Yuasa, T., Shimojo, H., Construction of nondefective adenovirus type 5 bearing a 2.8-kilobase hepatitis B virus DNA near the right end of its genome. J. Virol., 54, 711-719 (1985). PMID 3999192. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

References

Original, user report and related articles

original Miyake, S., Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. USA, 93, 1320-1324 (1996). PMID 8577762. [link to RRC of NBRP]
original Saito, I., Construction of nondefective adenovirus type 5 bearing a 2.8-kilobase hepatitis B virus DNA near the right end of its genome. J. Virol., 54, 711-719 (1985). PMID 3999192. [link to RRC of NBRP]
user_report Ugai H., Accumulation of infectious mutants in stocks during the propagation of fiber-modified recombinant adenoviruses. Biochem. Biophys. Res. Commun. 337, 806-814 (2005). PMID 16214112. [link to RRC of NBRP]
user_report Sato Y., Cell specificity and efficiency of the Semliki forest virus vector- and adenovirus vector-mediated gene expression in mouse cerebellum. J. Neurosci. Methods, 137, 111-121 (2004). PMID 15196833. [link to RRC of NBRP]