Shuttle vector to generate rAd expressing NtTA
| Catalog number | RDB01710 |
|---|---|
| Resource name | pAx1-CA-NtTA |
| Alternative name | CA-NtTA adenovirus vector |
| Clone info. | Shuttle vector to generate recombinant adenovirus expressing Tet transactivator containing nuclear localization signal under regulation with CA promoter. Produce adenovirus by COS-TPC method. The tTA which was from pUHD15-1 (Gossen, M., Bujard, H. PNAS, 89, 5547-5551, 1992) contains NLS. |
| Vector backbone | pAxcw (Cosmid, use packaging extracts for transformation) |
| Size of vector backbone | 42.5 kb |
| Selectable markers | Amp^r |
| Gene/insert name | E. coli Tet-transactivator Genomic DNA |
| Depositor | Hamada, Hirofumi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(J. Leukoc. Biol., 60, 181-190 (1996); Biochem. Biophys. Res. Commun., 230, 426-430 (1997).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
| Remarks | Remember that you will be working with samples containing infectious virus. |
[open/close]| Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
|---|---|---|---|---|
| RDB01710 | pAx1-CA-NtTA | Under QC test. Please contact us. | DNA solution |
Electronic file
| Electronic file | Map RDB01710.gif from Depositor
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| Electronic file | PDF (map, protocol and/or remarks) RDB01710.pdf from Depositor |
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Tet inducible system (English text) |
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This clone will be sequenced a portion and digested by restriction enzyme for examination.
Original, user report and related articles
2019.09.18
GNP_filter3_RDBDEP_html_190816.pl