Resource data sheet
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pAx1-CAh-tPA

Shuttle vector to generate rAd expressing human tissue plasminogen activator

Catalog number RDB01706
Resource name pAx1-CAh-tPA
Clone info. Shuttle vector to generate recombinant adenovirus vector expressing human tissue plasminogen activator gene. cDNA fragment 258 to 2138nt of NM_000930.4 (CDS, 258 to 1946nt) is cloned.
Use the COS-TPC method for production of adenovirus. Known difference (at least): C to T substitution at 758nt (Ala, synonym).
Vector backbone pAxcw (Cosmid, use packaging extracts for transformation)
Size of vector backbone 42.5 kb
Selectable markers Amp^r
Gene/insert name Human Tissue plasminogen activator (tPA) cDNA
Depositor Hamada, Hirofumi |
 
Other clones in our bank human PLAT (NCBI Gene 5327) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (J. Leukoc. Biol., 60, 181-190 (1996).) and an acknowledgment to the DEPOSITOR are requested.
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Remarks ((Additional form)) CAG(FormE)
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RDB01706 pAx1-CAh-tPA DNA solution

Ordering Information [in Japanese] [in English]

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Electronic file

Electronic file Map RDB01706.gif from Depositor

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References

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15035_A7Egp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB15035_A7Ega.seq
>01706_15035_A7Eg_2_pAxCA_F1_D05_11_ABI24.ab1
    1 CGTTCCCTTT CCTCCCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG
   61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCG CGCGTAATAC GACTCACTAT
  121 AGGGCGAATT GGGTACCGGG CCCCCCCTCG AGATGGATGC AATGAAGAGA GGGCTCTGCT
  181 GTGTGCTGCT GCTGTGTGGA GCAGTCTTCG TTTCGCCCAG CCAGGAAATC CATGCCCGAT
  241 TCAGAAGAGG AGCCAGATCT TACCAAGTGA TCTGCAGAGA TGAAAAAACG CAGATGATAT
  301 ACCAGCAACA TCAGTCATGG CTGCGCCCTG TGCTCAGAAG CAACCGGGTG GAATATTGCT
  361 GGTGCAACAG TGGCAGGGCA CAGTGCCACT CAGTGCCTGT CAAAAGTTGC AGCGAGCCAA
  421 GGTGTTTCAA CGGGGGCACC TGCCAGCAGG CCCTGTACTT CTCAGATTTC GTGTGCCAGT
  481 GCCCCGAAGG ATTTGCTGGG AAGTGCTGTG AAATAGATAC CAGGGCCACG TGCTACGAGG
  541 ACCAGGGCAT CAGCTACAGG GGCACGTGGA GCACAGCGGA GAGTGGCGCC GAGTGCACCA
  601 ACTGGAACAG CAGCGCGTTG GCCCAGAAGC CCTACAGCGG GCGGAGGCCA GATGCCATCA
  661 GGCTGGGCCT GGGGAACCAC AACTACTGCA GAAACCCAGA TCGAGACTCA AAGCCCTGGT
  721 GCTACGTCTT TAAGGCGGGG AAGTACAGCT CAGAGTTCTG CAGCACCCCT GCCTGCTCTG
  781 AGGGAAACAG TGACTGCTAC TTTGGGAATG GGTCAGCCTA CCGTGGCACG CACAGCCTCA
  841 CCGAGTCGGG TGCCTCCTGC CTCCCGTGGA ATTCCATGAT CCCTGAATAG GCAAGGTTTA
  901 CACAGCACAG AAACCCCAGG TGCCCAAGGC ACTGGGCCTG GGCAAACATA ATTACTGCCG
  961 GAATCCTGAT GGGGATGCCA AGCCCTTGTT GCCACGTGCT GAAGAACCGC AGCTGACGTG
 1021 GGAGTACTGG TGATGGTGCC CTCCTGCTCC ACCTGGCGGC TGGAGACAAG TACAGCCAGG
 1081 CTCAGGTTCC GCATTCAAGG AGGCTCTTCG GCGGACTCGC TCCAACCCCT GCAGCTGCCA
1141 TCATGCCAAG CCTGAAGTCG CTTTAGGTTC CTGTAGCGGG GCCAATAACC TCAA
//
Primer: pAxCA_R1 RDB15035_A7Egb.seq
>01706_15035_A7Eg_2_pAxCA_R1_C07_07_ABI24.ab1
    1 GGGGAACAGG GGCATTGGCC ACAACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA
   61 GGAGTGAATT CGCGCAATTA ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCACCGCGG
  121 TGGCGGCCGC TCTAGAACTA GTGGATCCCC CAAGTCCTGA AAACTTCCAA AATGGGAAGT
  181 ATCTGGGAAA ATGCACTCTT CCCTCTCCTG TAGGGTCTCG TCCCGCTTCT GCGGTGTGGT
  241 GGGTCTGGAG AAGTCTGTAG AGAAGCACTG CGCCTTTGCA GTGTCTTCTG AAGAAGAAGA
  301 GGCGGGATCT CATTTGCTTT TGAGGAGTCG GGTGTTCCTG GTCACGGTCG CATGTTGTCA
  361 CGAATCCAGT CTAGGTAGTT GGTAACCTTG GTGTACACAC CCGGGACATC CTTCTGTCCA
  421 CAGCCCAGGC CCCAGCTGAT GATGCCCACC AAAGTCATGC GGCCATCGTT CAGACACACC
  481 AGGGGGCCTC CCGAATCGCC CTGGCAGGCG TCGTGCAAGT TTGCCTGGGG CCCGCCGCTC
  541 CGAGTGTCTC CAGCACACAG CATGTTGTCG GTGACTGTTC TGTTAAGTAA ATGTTGTGAT
  601 GTGCAGCGGC TGGATGGGTA CAGTCTGACA TGAGCCTCCT TCAGCCGCTC CGAATAGAAA
  661 GGAGACAAGG CCTCATGCTT GCCGTAGCCG GAGAGCTCAC ACTCCGTCCA GTCCGGCAGC
  721 TGCAGGTCCG CCGGGGGAAG GCACACAGTG CGGACCACGC TGCTCTCCTG GGCACAGCGG
  781 GACGAATCCG ATTTCAGCTG CAGCAGCGCA ATGTCATTGT CGTAAGTGTC ATCATCGAAT
  841 TCCTTATGGA CAATGTATTT TTCGACTTCA AATTTCTGCT CCTCCTCGCC AGGGACCACC
  901 CGGTATGTTC TGCCCAAGAT CACCGTCAGG TGGTGGGGCG GAAACCTCTC CTGGAAGCAG
  961 TGGGCGGCAG AGAGAATCCA GCAGGAGCTG ATGAGTATGC CCCCGCACAG GAACCGCTCT
 1021 CCGGGCGACC TCCTGTGCTT TGCAAAGATG GCAGCCTGCC CAGGGGTGGG AGGCGATGTC
 1081 GGCGAAGAAG CCCTCTTTTG ATGCGAACTG AGCCTGGGCT TGTACTGTCT CAAGGCGGCA
1141 AGTGGAAGCA GGAGGCACAT CCACAGTTTA ACTCTCCCAC ACGGTTTCAG GCCCC
//
Primer: pAxcwit2-Fw RDB15035_A7Egc.seq
>01706_15035_A7Eg_2_pAxcwit2_Fw_A07_01_ABI24.ab1
    1 CACTAGCGCG GGGTGCAGGC CAGCACCAAG TGATCGGGCC TCAGCTCCTC GGTCACATCC
   61 AGCATCACAG GCTGGTTCCT AATATGTTTA CCGCCACACT CGCAGGGTCT GCACCTGGTG
  121 CGGGTCTCAT CGTACCTCAG CACCTTCCAG ATCCATCGTC GCGAGTCGAC AATCGATTTT
  181 CGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC
  241 CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC
  301 AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG
  361 ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT
  421 CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC
  481 TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA
  541 TTAGTCATCG CTATTACCAT GGTCGAGGTG AGCCCCACGT TCTGCTTCAC TCTCCCCATC
  601 TCCCCCCCCT CCCCACCCCC AATTTTGTAT TTATTTATTT TTTAATTATT TTGTGCAGCG
  661 ATGGGGGCGG GGGGGGGGGG GGGGCGCGCG CCAGGCGGGG CGGGGCGGGG CGAGGGGCGG
  721 GGCGGGGCGA GGCGGAGAGG TGCGGCGGCA GCCAATCAGA GCGGCGCGCT CCGAAAGTTT
781 CCTTTTATGG GCGAGG
//
Primer: Amp_R RDB15035_A7Egd.seq
>01706_15035_A7Eg_2_Amp_R_B07_04_ABI24.ab1
    1 CTCGTTTCAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
  181 AGGCCCTTTC GTCTTCAAGA ATTCCGGATC CGGGCCAATA TGATAATGAG GGGGTGGAGT
  241 TTGTGACGTG GCGCGGGGCG TGGGAACGGG GCGGGTGACG TAGTAGTGTG GCGGAAGTGT
  301 GATGTTGCAA GTGTGGCGGA ACACATGTAA GCGACGGATG TGGCAAAAGT GACGTTTTTG
  361 GTGTGCGCCG GTGTACACAG GAAGTGACAA TTTTCGCGCG GTTTTAGGCG GATGTTGTAG
  421 TAAATTTGGG CGTAACCGAG TAAGATTTGG CCATTTTCGC GGGAAAACTG AATAAGAGGA
  481 AGTGAAATCT GAATAATTTT GTGTTACTCA TAGCGCGTAA TATTTGTCTA GGGCCGCGGG
  541 GACTTTGACC GTTTACGTGG AGACTCGCCC AGGTGTTTTT CTCAGGTGTT TTCCGCGTTC
  601 CGGGTCAAAG TTGGCGTTTT ATTATTATAG TCAGCATCGA TTAGCTTGGG CTGCAGGTCG
  661 AGGGATCTCC ATAAGAGAAG AGGGACAGCT ATGACTGGGA GTAGTCAGGA GAGGAGGAAA
  721 AATCTGGCTA GTAAAACATG TAAGGAAAAT TTTAGGGATG TTAAAGAAAA AAATAACACA
  781 AAACAAAATA TAAAAAAAAT CTAACCTCAA GTCAAGGCTT TTCTATGGAA TAAGGAATGG
  841 ACAGCAGGGG GCTGTTTCAT ATACTGATGA CCTCTTTATA GCCAACCTTT GTTCATGGCA
  901 GCCAGCATAT GGGCATATGT TGCCAAACTC TAAACCAAAT ACTCATTCTG ATGTTTTAAA
  961 TGATTTGCCC TCCATATGTC CTTCCGAGTG AGAGACACAA AAAATTCCAA CACACTATTG
 1021 CAATGAAAAT AAATTTCTTT ATTAGCCAGA AGTCAGATGC TCAGGGCCTC ATGATGTCCC
 1081 CATAATTTTG CGAGGGAAAA GATCTCAGTG TATTGTGAAC CAAGCAATGG ACAACAAGCA
1141 ACAACATTCT GAAATAGGCA GGCCTGGCAC
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

GNP_filter3_RDBDEP_html_181012.pl