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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAx1-CAh-tPA

Shuttle vector to generate rAd expressing human tissue plasminogen activator

Catalog number RDB01706
Resource name pAx1-CAh-tPA
Clone info. Shuttle vector to generate recombinant adenovirus vector expressing human tissue plasminogen activator gene. cDNA fragment 258 to 2138nt of NM_000930.4 (CDS, 258 to 1946nt) is cloned.
Comment Use the COS-TPC method for production of adenovirus. Known difference (at least): C to T substitution at 758nt (Ala, synonym).
Vector backbone pAxcw (Cosmid, use packaging extracts for transformation)
Size of vector backbone 42.5 kb
Selectable markers Amp^r
Gene/insert name Human Tissue plasminogen activator (tPA) cDNA
Depositor|Developer Hamada, Hirofumi |
Other clones in our bank

External Database
Human Tissue plasminogen activator (tPA)

          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB01706.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (J. Leukoc. Biol., 60, 181-190 (1996).) and an acknowledgment to the DEPOSITOR are requested.
          Additional terms and conditions:
          Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献 (J. Leukoc. Biol., 60, 181-190 (1996).) を引用し、謝辞の表明を必要とする。
          付加的提供条件:
          CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB01706 pAx1-CAh-tPA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15035_A7Egp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Sequence file: RDB15035_A7Ega.seq check
          >01706_15035_A7Eg_2_pAxCA_F1_D05_11_ABI24.ab1
              1 CGTTCCCTTT CCTCCCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG
             61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCG CGCGTAATAC GACTCACTAT
            121 AGGGCGAATT GGGTACCGGG CCCCCCCTCG AGATGGATGC AATGAAGAGA GGGCTCTGCT
            181 GTGTGCTGCT GCTGTGTGGA GCAGTCTTCG TTTCGCCCAG CCAGGAAATC CATGCCCGAT
            241 TCAGAAGAGG AGCCAGATCT TACCAAGTGA TCTGCAGAGA TGAAAAAACG CAGATGATAT
            301 ACCAGCAACA TCAGTCATGG CTGCGCCCTG TGCTCAGAAG CAACCGGGTG GAATATTGCT
            361 GGTGCAACAG TGGCAGGGCA CAGTGCCACT CAGTGCCTGT CAAAAGTTGC AGCGAGCCAA
            421 GGTGTTTCAA CGGGGGCACC TGCCAGCAGG CCCTGTACTT CTCAGATTTC GTGTGCCAGT
            481 GCCCCGAAGG ATTTGCTGGG AAGTGCTGTG AAATAGATAC CAGGGCCACG TGCTACGAGG
            541 ACCAGGGCAT CAGCTACAGG GGCACGTGGA GCACAGCGGA GAGTGGCGCC GAGTGCACCA
            601 ACTGGAACAG CAGCGCGTTG GCCCAGAAGC CCTACAGCGG GCGGAGGCCA GATGCCATCA
            661 GGCTGGGCCT GGGGAACCAC AACTACTGCA GAAACCCAGA TCGAGACTCA AAGCCCTGGT
            721 GCTACGTCTT TAAGGCGGGG AAGTACAGCT CAGAGTTCTG CAGCACCCCT GCCTGCTCTG
            781 AGGGAAACAG TGACTGCTAC TTTGGGAATG GGTCAGCCTA CCGTGGCACG CACAGCCTCA
            841 CCGAGTCGGG TGCCTCCTGC CTCCCGTGGA ATTCCATGAT CCCTGAATAG GCAAGGTTTA
            901 CACAGCACAG AAACCCCAGG TGCCCAAGGC ACTGGGCCTG GGCAAACATA ATTACTGCCG
            961 GAATCCTGAT GGGGATGCCA AGCCCTTGTT GCCACGTGCT GAAGAACCGC AGCTGACGTG
           1021 GGAGTACTGG TGATGGTGCC CTCCTGCTCC ACCTGGCGGC TGGAGACAAG TACAGCCAGG
           1081 CTCAGGTTCC GCATTCAAGG AGGCTCTTCG GCGGACTCGC TCCAACCCCT GCAGCTGCCA
          1141 TCATGCCAAG CCTGAAGTCG CTTTAGGTTC CTGTAGCGGG GCCAATAACC TCAA
          //
          Primer: pAxCA_R1
          Sequence file: RDB15035_A7Egb.seq check
          >01706_15035_A7Eg_2_pAxCA_R1_C07_07_ABI24.ab1
              1 GGGGAACAGG GGCATTGGCC ACAACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA
             61 GGAGTGAATT CGCGCAATTA ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCACCGCGG
            121 TGGCGGCCGC TCTAGAACTA GTGGATCCCC CAAGTCCTGA AAACTTCCAA AATGGGAAGT
            181 ATCTGGGAAA ATGCACTCTT CCCTCTCCTG TAGGGTCTCG TCCCGCTTCT GCGGTGTGGT
            241 GGGTCTGGAG AAGTCTGTAG AGAAGCACTG CGCCTTTGCA GTGTCTTCTG AAGAAGAAGA
            301 GGCGGGATCT CATTTGCTTT TGAGGAGTCG GGTGTTCCTG GTCACGGTCG CATGTTGTCA
            361 CGAATCCAGT CTAGGTAGTT GGTAACCTTG GTGTACACAC CCGGGACATC CTTCTGTCCA
            421 CAGCCCAGGC CCCAGCTGAT GATGCCCACC AAAGTCATGC GGCCATCGTT CAGACACACC
            481 AGGGGGCCTC CCGAATCGCC CTGGCAGGCG TCGTGCAAGT TTGCCTGGGG CCCGCCGCTC
            541 CGAGTGTCTC CAGCACACAG CATGTTGTCG GTGACTGTTC TGTTAAGTAA ATGTTGTGAT
            601 GTGCAGCGGC TGGATGGGTA CAGTCTGACA TGAGCCTCCT TCAGCCGCTC CGAATAGAAA
            661 GGAGACAAGG CCTCATGCTT GCCGTAGCCG GAGAGCTCAC ACTCCGTCCA GTCCGGCAGC
            721 TGCAGGTCCG CCGGGGGAAG GCACACAGTG CGGACCACGC TGCTCTCCTG GGCACAGCGG
            781 GACGAATCCG ATTTCAGCTG CAGCAGCGCA ATGTCATTGT CGTAAGTGTC ATCATCGAAT
            841 TCCTTATGGA CAATGTATTT TTCGACTTCA AATTTCTGCT CCTCCTCGCC AGGGACCACC
            901 CGGTATGTTC TGCCCAAGAT CACCGTCAGG TGGTGGGGCG GAAACCTCTC CTGGAAGCAG
            961 TGGGCGGCAG AGAGAATCCA GCAGGAGCTG ATGAGTATGC CCCCGCACAG GAACCGCTCT
           1021 CCGGGCGACC TCCTGTGCTT TGCAAAGATG GCAGCCTGCC CAGGGGTGGG AGGCGATGTC
           1081 GGCGAAGAAG CCCTCTTTTG ATGCGAACTG AGCCTGGGCT TGTACTGTCT CAAGGCGGCA
          1141 AGTGGAAGCA GGAGGCACAT CCACAGTTTA ACTCTCCCAC ACGGTTTCAG GCCCC
          //
          Primer: pAxcwit2-Fw
          Sequence file: RDB15035_A7Egc.seq check
          >01706_15035_A7Eg_2_pAxcwit2_Fw_A07_01_ABI24.ab1
              1 CACTAGCGCG GGGTGCAGGC CAGCACCAAG TGATCGGGCC TCAGCTCCTC GGTCACATCC
             61 AGCATCACAG GCTGGTTCCT AATATGTTTA CCGCCACACT CGCAGGGTCT GCACCTGGTG
            121 CGGGTCTCAT CGTACCTCAG CACCTTCCAG ATCCATCGTC GCGAGTCGAC AATCGATTTT
            181 CGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC
            241 CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC
            301 AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG
            361 ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT
            421 CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC
            481 TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA
            541 TTAGTCATCG CTATTACCAT GGTCGAGGTG AGCCCCACGT TCTGCTTCAC TCTCCCCATC
            601 TCCCCCCCCT CCCCACCCCC AATTTTGTAT TTATTTATTT TTTAATTATT TTGTGCAGCG
            661 ATGGGGGCGG GGGGGGGGGG GGGGCGCGCG CCAGGCGGGG CGGGGCGGGG CGAGGGGCGG
            721 GGCGGGGCGA GGCGGAGAGG TGCGGCGGCA GCCAATCAGA GCGGCGCGCT CCGAAAGTTT
          781 CCTTTTATGG GCGAGG
          //
          Primer: Amp_R
          Sequence file: RDB15035_A7Egd.seq check
          >01706_15035_A7Eg_2_Amp_R_B07_04_ABI24.ab1
              1 CTCGTTTCAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
             61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
            121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
            181 AGGCCCTTTC GTCTTCAAGA ATTCCGGATC CGGGCCAATA TGATAATGAG GGGGTGGAGT
            241 TTGTGACGTG GCGCGGGGCG TGGGAACGGG GCGGGTGACG TAGTAGTGTG GCGGAAGTGT
            301 GATGTTGCAA GTGTGGCGGA ACACATGTAA GCGACGGATG TGGCAAAAGT GACGTTTTTG
            361 GTGTGCGCCG GTGTACACAG GAAGTGACAA TTTTCGCGCG GTTTTAGGCG GATGTTGTAG
            421 TAAATTTGGG CGTAACCGAG TAAGATTTGG CCATTTTCGC GGGAAAACTG AATAAGAGGA
            481 AGTGAAATCT GAATAATTTT GTGTTACTCA TAGCGCGTAA TATTTGTCTA GGGCCGCGGG
            541 GACTTTGACC GTTTACGTGG AGACTCGCCC AGGTGTTTTT CTCAGGTGTT TTCCGCGTTC
            601 CGGGTCAAAG TTGGCGTTTT ATTATTATAG TCAGCATCGA TTAGCTTGGG CTGCAGGTCG
            661 AGGGATCTCC ATAAGAGAAG AGGGACAGCT ATGACTGGGA GTAGTCAGGA GAGGAGGAAA
            721 AATCTGGCTA GTAAAACATG TAAGGAAAAT TTTAGGGATG TTAAAGAAAA AAATAACACA
            781 AAACAAAATA TAAAAAAAAT CTAACCTCAA GTCAAGGCTT TTCTATGGAA TAAGGAATGG
            841 ACAGCAGGGG GCTGTTTCAT ATACTGATGA CCTCTTTATA GCCAACCTTT GTTCATGGCA
            901 GCCAGCATAT GGGCATATGT TGCCAAACTC TAAACCAAAT ACTCATTCTG ATGTTTTAAA
            961 TGATTTGCCC TCCATATGTC CTTCCGAGTG AGAGACACAA AAAATTCCAA CACACTATTG
           1021 CAATGAAAAT AAATTTCTTT ATTAGCCAGA AGTCAGATGC TCAGGGCCTC ATGATGTCCC
           1081 CATAATTTTG CGAGGGAAAA GATCTCAGTG TATTGTGAAC CAAGCAATGG ACAACAAGCA
          1141 ACAACATTCT GAAATAGGCA GGCCTGGCAC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.10.12

          GNP_filter3_RDBDEP_html_221010.pl