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pRx hFAS-i-hCD25 (#RDB01702)

Retroviral vector of hFAS (CD95) and hCD25 (IL2R alpha).

Alternative name CD95 and CD25
Clone info. Retroviral vector of hFAS (CD95) and hCD25 (IL2R alpha) as a surface marker.
Vector backbone pRx (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli)
Gene/insert name Human FAS (CD95) cDNA Human CD25 cDNA
Depositor|Developer Hamada, Hirofumi |
 
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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (J. Leukoc. Biol., 60, 181-190 (1996).) and an acknowledgment to the DEPOSITOR are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献 (J. Leukoc. Biol., 60, 181-190 (1996).) を引用し、謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01702 pRx hFAS-i-hCD25 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pRx hFAS-i-hCD25 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01702).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15080_A7Epp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: hFAS-M_R
Sequence file: RDB15080_A7Epa.seq check
>01702_15080_A7Ep_hFAS-M_R_B04_04_ABI24.ab1
    1 GTGTATACAG GTAGAGTTAC AAAAAAAGTT TGGTTTACAT CTGCACTTGG TATTCTGGGT
   61 CCGGGTGCAG TTTATTTCCA CTTCTAAGCC ATGTCCTTCA TCACACAATC TACATCTTCT
  121 GCATTTGGAA GAAAAATGGG CTTTGTCTGT GTACTCCTTC CCTTCTTGGC AGGGCACGCA
  181 GTCTGGTTCA TCCCCATTGA CTGTGCAGTC CCTAGCTTTC CTTTCACCTG GAGGACAGGG
  241 CTTATGGCAG AATTGGCCAT CATGATGCAG GCCTTCCAAG TTCTGAGTCT CAACTGTAGT
  301 AACAGTCTTC CTCAATTCCA ATCCCTTGGA GTTGATGTCA GTCACTTGGG CATTAACACT
  361 TTTGGACGAT AATCTAGCAA CAGACGTAAG AACCAGAGGT AGGAGGGTCC AGATGCCCAG
  421 CATGGTGCTC GAGAATTCGA TATCAAGCTT ATCGATACCG TCGACGCCCT ATAGTGAGTC
  481 GTATTACTCT AGAGGATGGT CCACCCCCGG GGTCGGCAGC CTTCACGTGG GCGGCGTGTA
  541 TCCAAGCTGC GATGCCGTCT ACTTTGAGGG CGGTGGGGGT GGTCAGCAGG ACTGTGTAAG
  601 GTCCTTTCCA GCGAGGTTCT AGGTTCTTAG TCTGGTGTCG GCGGACCCAC ACTGTGTCGC
  661 CGACTCGGTA AGGGTGAGGT ACCACCGGTC GGTCCAGTTG TTCTTGGTAG GCTGCCGCCA
  721 GAGGTCTCCA GACTTCGTGC TGGACTAAGT AGAGAGCCTG TAAGTGAGCT TGGAGAGAGG
  781 GGCTGTTAGT AACTCTTGTC ATGTCAGGGT CAGGGAAGTT TACAAGGGGC GGGGGTGCCC
  841 CATATAAGAT CTCATATGGC CATATGGGGG CGCCTAGAGA AGGAGTGAGG GCTGGATAAA
  901 GGGAGGATCG AGGCGGGGTC GAACGAGAGG TTCAAGGGGG AGAGACGGGC GGATGGAGGA
  961 AGAGGAGCGG AGGCTTTAGG GTGTACAAAG GGCTTTGACC CAGGGAGGGG GGTCAAAAGC
 1021 CAAAGCTTCC AGGTCACGAT GTAGGACCTG ATCTGGGTGT CCATGCAGCA GGTGAAAAGA
 1081 CTTGATCTAC CTGGGTGATG AAGTCTCGGT AAGGTGCCGT CTTCGCGGGC CAATTTCGCG
//
Primer: hFAS-M_F
Sequence file: RDB15080_A7Epb.seq check
>01702_15080_A7Ep_hFAS-M_F_A04_01_ABI24.ab1
    1 TCAAGGGACC AGATACCAGT GCAGATGTAA ACCAAACTTT TTTTGTAACT CTACTGTATG
   61 TGAACACTGT GACCCTTGCA CCAAATGTGA ACATGGAATC ATCAAGGAAT GCACACTCAC
  121 CAGCAACACC AAGTGCAAAG AGGAAGGATC CAGATCTAAC TTGGGGTGGC TTTGTCTTCT
  181 TCTTTTGCCA ATTCCACTAA TTGTTTGGGT GAAGAGAAAG GAAGTACAGA AAACATGCAG
  241 AAAGCACAGA AAGGAAAACC AAGGTTCTCA TGAATCTCCA ACCTTAAATC CTGAAACAGT
  301 GGCAATAAAT TTATCTGATG TTGACTTGAG TAAATATATC ACCACTATTG CTGGAGTCAT
  361 GACACTAAGT CAAGTTAAAG GCTTTGTTCG AAAGAATGGT GTCAATGAAG CCAAAATAGA
  421 TGAGATCAAG AATGACAATG TCCAAGACAC AGCAGAACAG AAAGTTCAAC TGCTTCGTAA
  481 TTGGCATCAA CTTCATGGAA AGAAAGAAGC GTATGACACA TTGATTAAAG ATCTCAAAAA
  541 AGCCAATCTT TGTACTCTTG CAGAGAAAAT TCAGACTATC ATCCTCAAGG ACATTACTAG
  601 TGACTCAGAA AATTCAAACT TCAGAAATGA AATCCAAAGC TTGGTCTAGA ATTCCTGCAG
  661 CCCGGGGGAT CCACTAGTTC TAGAGCGGCC GCAGTATTCT ATAGTGTCAC AATGGATCCT
721 CTAGAGAATT CCGCCCCCCC CCCCACCCCC CCTCACC
//
Primer: IRES-F
Sequence file: RDB15080_A7Epc.seq check
>01702_15080_A7Ep_IRES-F_H07_22_ABI24.ab1
    1 AGCAAGTCAT TTCATGTGTT TAGTCGAGGT TAAAAAACGT CTAGGCCCCC CGAACCACGG
   61 GGACGTGGTT TTCCTTTGAA AAACACGATG ATAAGCTTGC CACGACGCGT CTCGACCATT
  121 GTGCTGGAAG ATGGATTCAT ACCTGCTGAT GTGGGGACTG CTCACGTTCA TCATGGTGCC
  181 TGGCTGCCAG GCAGAGCTCT GTGACGATGA CCCGCCAGAG ATCCCACACG CCACATTCAA
  241 AGCCATGGCC TACAAGGAAG GAACCATGTT GAACTGTGAA TGCAAGAGAG GTTTCCGCAG
  301 AATAAAAAGC GGGTCACTCT ATATGCTCTG TACAGGAAAC TCTAGCCACT CGTCCTGGGA
  361 CAACCAATGT CAATGCACAA GCTCTGCCAC TCGGAACACA ATGAAACAAG TGACACCTCA
  421 ACCTGAAGAA CAGAAAGAAA GGAAAACCAC AGAAATGCAA AGTCCAATGC AGCCAGTGGA
  481 CCAAGCGAGC CTTCCAGGTC ACTGCAGGGA ACCTCCACCA TGGGAAAATG AAGCCACAGA
  541 GAGAATTTAT CATTTCGTGG TGGGGCAGAT GGTTTATTAT CAGTGCGTCC AGGGATACAG
  601 GGCTCTACAC AGAGGTCCTG CTGAGAGCGT CTGCAAAATG ACCCACGGGA AGACAAGGTG
  661 GACCCAGCCC CAGCTCATAT GCACAGGTGA AATGGAGACC AGTCAGTTTC CAGGTGAAGA
  721 GAAGCCTCAG GCAAGCCCCG AAGGCCGTCC TGAGAGTGAG ACTTCCTGCC TCGTCACAAC
  781 AACAGATTTT CAAATACAGA CAGAAATGGC TGCAACCATG GAGACGTCCA TATTTACAAC
  841 AGAGTACCAG GTAGCAGTGG CCGGCTGTGT TTTCCTGCTG ATCAGCGTCC TCCTCCTGAG
  901 TGGGCTCACC TGGCAGCGGA GACAGAGGAA GAGTAGAAGA ACAATCTAGC CAGCACAATG
  961 GCGGCCGACG CGTCCCGGAG CCATAGATAA AATAAAAAGA TTTTTATTTA GTCTCCAGAA
 1021 AAAGGGGGGA ATGAAAGACC CCCACCTGTA GGTTGGCAGC TAGCTTAAGG TAACGCCATT
 1081 TTTGCAGCAT GAAAAAATAA CATAACTGAG AATAGAGTAG TCAGATTCCA GATCAAGAAC
1141 AGAATGGAAC AAAGCCTGTG AATTATTATT GGGGTC
//
Primer: C-LTR-R
Sequence file: RDB15080_A7Epd.seq check
>01702_15080_A7Ep_C-LTR_R_B09_06_ABI24.ab1
    1 GCGGCATCTT ACCAGATATC CTGTTTGGCC CATATTCAGC TGTTCCATCT GTTCTTGACC
   61 TTGATCTGAA CTTCTCTATT CTCAGTTATG TATTTTTCCA TGCCTTGCAA AATGGCGTTA
  121 CTTAAGCTAG CTTGCCAAAC CTACAGGTGG GGTCTTTCAT TCCCCCCTTT TTCTGGAGAC
  181 TAAATAAAAT CTTTTATTTT ATCTATGGCT CCGGACGCGT CGGCCGCCAT TGTGCTGGCT
  241 AGATTGTTCT TCTACTCTTC CTCTGTCTCC GCTGCCAGGT GAGCCCACTC AGGAGGAGGA
  301 CGCTGATCAG CAGGAAAACA CAGCCGGCCA CTGCTACCTG GTACTCTGTT GTAAATATGG
  361 ACGTCTCCAT GGTTGCAGCC ATTTCTGTCT GTATTTGAAA ATCTGTTGTT GTGACGAGGC
  421 AGGAAGTCTC ACTCTCAGGA CGGCCTTCGG GGCTTGCCTG AGGCTTCTCT TCACCTGGAA
  481 ACTGACTGGT CTCCATTTCA CCTGTGCATA TGAGCTGGGG CTGGGTCCAC CTTGTCTTCC
  541 CGTGGGTCAT TTTGCAGACG CTCTCAGCAG GACCTCTGTG TAGAGCCCTG TATCCCTGGA
  601 CGCACTGATA ATAAACCATC TGCCCCACCA CGAAATGATA AATTCTCTCT GTGGCTTCAT
  661 TTTCCCATGG TGGAGGTTCC CTGCAGTGAC CTGGAAGGCT CGCTTGGTCC ACTGGCTGCA
  721 TTGGACTTTG CATTTCTGTG GTTTTCCTTT CTTTCTGTTC TTCAGGTTGA GGTGTCACTT
  781 GTTTCATTGT GTTCCGAGTG GCAGAGCTTG TGCATTGACA TTGGTTGTCC CAGGACGAGT
  841 GGCTAGAGTT TCCTGTACAG AGCATATAGA GTGACCCGCT TTTTATTCTG CGGAAACCTC
  901 TCTTGCATTC ACAGTTCAAC ATGGTTCCTT CCTTGTAGGC CATGGCTTTT GAATGTGGCG
961 TGTGGGAATC TCTGGC
//
Primer: pMFG_1
Sequence file: RDB15080_A7Epe.seq check
>01702_15080_A7Ep_pMFG-1_A02_01_ABI08.ab1
    1 GCAGGGGGGG GGAGGGGCTG TTAGTAACTC TTGTCATGTC AGGGTCAGGG AAGTTTACAA
   61 GGGGCGGGGG TGCCCCATAT AAGATCTCAT ATGGCCATAT GGGGGCGCCT AGAGAAGGAG
  121 TGAGGGCTGG ATAAAGGGAG GATCGAGGCG GGGTCGAACG AGGAGGTTCA AGGGGGAGAG
  181 ACGGGGCGGA TGGAGGAAGA GGAGGCGGAG GCTTAGGGTG TACAAAGGGC TTGACCCAGG
  241 GAGGGGGGTC AAAAGCCAAG GCTTCCCAGG TCACGATGTA GGGGACCTGG TCTGGGTGTC
  301 CATGCGGGCC AGGTGAAAAG ACCTTGATCT TAACCTGGGT GATGAGGTCT CGGTTAAAGG
  361 TGCCGTCTCG CGGCCATCCG ACGTTAAAGG TTGGCCATTC TGCAGAGCAG AAGGTAACCC
  421 AACGTCTCTT CTTGACATCT ACCGACTGGT TGTGAGCGAT CCGCTCGACA TCTTTCCAGT
  481 GACCTAAGGT CAAACTTAAG GGAGTGGTAA CAGTCTGGCC CGGGCCCATA TTTTCAGACA
  541 AATACAGAAA CACAGTCAGA CAGAGACAAC ACAGAACGAT GCTGCAGCAG ACAAGACGCG
  601 CGGCGCGGCT TCGGTCCCAA ACCGAAAGCA AAAATTCAGA CGGAGGCGGG AACTGTTTTA
  661 GGTTCTCGTC TCCTACCAGA ACCACATATC CCTCCTCTAA GGGGGGTGCA CCAAAGAGTC
  721 CAAAACGATC GGGATTTTTG GACTCAGGTC GGGCCACAAA AACGGCCCCC GAAGTCCCTG
  781 GGACGTCTCC CAGGGTTGCG GCCGGGTGTT CCGAACTCGT CAGTTCCACC ACGGGTCCGC
  841 CAGATACAGA GCTAGTTAGC TAACTAGTAC CGACGCAGGC GCATAAAATC AGTCATAGAC
  901 ACTAGACAAA TCGGACAGAC ACAGATAAGT TGCTGGCCAG CTTACCCTCC CGGGTGGTGG
  961 GGTCGGGTGG TCCCTGGGGC AAGGGGTCTC CCGAATCCCG GGACGAGCCC CCCGAAATGA
 1021 AAGACCCCCG CTGACTGGTA GTTCAATCAC TTCAGAGGAG ACCCCCTTCC CTAGACAGCT
1081 AGACCAACCA AGTCGGAATG GCCAACCGTG CA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Wakimoto, H., Efficient retrovirus-mediated cytokine-gene transduction of primary-cultured human glioma cells for tumor vaccination therapy. Jpn. J. Cancer Res., 88, 296-305 (1997). PMID 9140115.
user_report Tone, Y., Death signalobody: inducing conditional cell death in response to a specific antigen. Hum. Gene Ther. Methods 24 (3):141-150 (2013). PMID 23470213. [link to RRC of NBRP]