Resource data sheet
Prev.

pRx hFAS-i-hCD25

Retroviral vector of hFAS (CD95) and hCD25 (IL2R alpha).

Catalog number RDB01702
Resource name pRx hFAS-i-hCD25
Alternative name CD95 and CD25
Clone info. Retroviral vector of hFAS (CD95) and hCD25 (IL2R alpha) as a surface marker.
Vector backbone pRx (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli)
Gene/insert name Human FAS (CD95) cDNA Human CD25 cDNA
Depositor Hamada, Hirofumi |
 
Other clones in our bank human FAS (NCBI Gene 355) | human IL2RA (NCBI Gene 3559) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (J. Leukoc. Biol., 60, 181-190 (1996).) and an acknowledgment to the DEPOSITOR are requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01702 pRx hFAS-i-hCD25 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01702.gif from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15080_A7Epp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: hFAS-M_R RDB15080_A7Epa.seq
>01702_15080_A7Ep_hFAS-M_R_B04_04_ABI24.ab1
    1 GTGTATACAG GTAGAGTTAC AAAAAAAGTT TGGTTTACAT CTGCACTTGG TATTCTGGGT
   61 CCGGGTGCAG TTTATTTCCA CTTCTAAGCC ATGTCCTTCA TCACACAATC TACATCTTCT
  121 GCATTTGGAA GAAAAATGGG CTTTGTCTGT GTACTCCTTC CCTTCTTGGC AGGGCACGCA
  181 GTCTGGTTCA TCCCCATTGA CTGTGCAGTC CCTAGCTTTC CTTTCACCTG GAGGACAGGG
  241 CTTATGGCAG AATTGGCCAT CATGATGCAG GCCTTCCAAG TTCTGAGTCT CAACTGTAGT
  301 AACAGTCTTC CTCAATTCCA ATCCCTTGGA GTTGATGTCA GTCACTTGGG CATTAACACT
  361 TTTGGACGAT AATCTAGCAA CAGACGTAAG AACCAGAGGT AGGAGGGTCC AGATGCCCAG
  421 CATGGTGCTC GAGAATTCGA TATCAAGCTT ATCGATACCG TCGACGCCCT ATAGTGAGTC
  481 GTATTACTCT AGAGGATGGT CCACCCCCGG GGTCGGCAGC CTTCACGTGG GCGGCGTGTA
  541 TCCAAGCTGC GATGCCGTCT ACTTTGAGGG CGGTGGGGGT GGTCAGCAGG ACTGTGTAAG
  601 GTCCTTTCCA GCGAGGTTCT AGGTTCTTAG TCTGGTGTCG GCGGACCCAC ACTGTGTCGC
  661 CGACTCGGTA AGGGTGAGGT ACCACCGGTC GGTCCAGTTG TTCTTGGTAG GCTGCCGCCA
  721 GAGGTCTCCA GACTTCGTGC TGGACTAAGT AGAGAGCCTG TAAGTGAGCT TGGAGAGAGG
  781 GGCTGTTAGT AACTCTTGTC ATGTCAGGGT CAGGGAAGTT TACAAGGGGC GGGGGTGCCC
  841 CATATAAGAT CTCATATGGC CATATGGGGG CGCCTAGAGA AGGAGTGAGG GCTGGATAAA
  901 GGGAGGATCG AGGCGGGGTC GAACGAGAGG TTCAAGGGGG AGAGACGGGC GGATGGAGGA
  961 AGAGGAGCGG AGGCTTTAGG GTGTACAAAG GGCTTTGACC CAGGGAGGGG GGTCAAAAGC
 1021 CAAAGCTTCC AGGTCACGAT GTAGGACCTG ATCTGGGTGT CCATGCAGCA GGTGAAAAGA
 1081 CTTGATCTAC CTGGGTGATG AAGTCTCGGT AAGGTGCCGT CTTCGCGGGC CAATTTCGCG
//
Primer: hFAS-M_F RDB15080_A7Epb.seq
>01702_15080_A7Ep_hFAS-M_F_A04_01_ABI24.ab1
    1 TCAAGGGACC AGATACCAGT GCAGATGTAA ACCAAACTTT TTTTGTAACT CTACTGTATG
   61 TGAACACTGT GACCCTTGCA CCAAATGTGA ACATGGAATC ATCAAGGAAT GCACACTCAC
  121 CAGCAACACC AAGTGCAAAG AGGAAGGATC CAGATCTAAC TTGGGGTGGC TTTGTCTTCT
  181 TCTTTTGCCA ATTCCACTAA TTGTTTGGGT GAAGAGAAAG GAAGTACAGA AAACATGCAG
  241 AAAGCACAGA AAGGAAAACC AAGGTTCTCA TGAATCTCCA ACCTTAAATC CTGAAACAGT
  301 GGCAATAAAT TTATCTGATG TTGACTTGAG TAAATATATC ACCACTATTG CTGGAGTCAT
  361 GACACTAAGT CAAGTTAAAG GCTTTGTTCG AAAGAATGGT GTCAATGAAG CCAAAATAGA
  421 TGAGATCAAG AATGACAATG TCCAAGACAC AGCAGAACAG AAAGTTCAAC TGCTTCGTAA
  481 TTGGCATCAA CTTCATGGAA AGAAAGAAGC GTATGACACA TTGATTAAAG ATCTCAAAAA
  541 AGCCAATCTT TGTACTCTTG CAGAGAAAAT TCAGACTATC ATCCTCAAGG ACATTACTAG
  601 TGACTCAGAA AATTCAAACT TCAGAAATGA AATCCAAAGC TTGGTCTAGA ATTCCTGCAG
  661 CCCGGGGGAT CCACTAGTTC TAGAGCGGCC GCAGTATTCT ATAGTGTCAC AATGGATCCT
721 CTAGAGAATT CCGCCCCCCC CCCCACCCCC CCTCACC
//
Primer: IRES-F RDB15080_A7Epc.seq
>01702_15080_A7Ep_IRES-F_H07_22_ABI24.ab1
    1 AGCAAGTCAT TTCATGTGTT TAGTCGAGGT TAAAAAACGT CTAGGCCCCC CGAACCACGG
   61 GGACGTGGTT TTCCTTTGAA AAACACGATG ATAAGCTTGC CACGACGCGT CTCGACCATT
  121 GTGCTGGAAG ATGGATTCAT ACCTGCTGAT GTGGGGACTG CTCACGTTCA TCATGGTGCC
  181 TGGCTGCCAG GCAGAGCTCT GTGACGATGA CCCGCCAGAG ATCCCACACG CCACATTCAA
  241 AGCCATGGCC TACAAGGAAG GAACCATGTT GAACTGTGAA TGCAAGAGAG GTTTCCGCAG
  301 AATAAAAAGC GGGTCACTCT ATATGCTCTG TACAGGAAAC TCTAGCCACT CGTCCTGGGA
  361 CAACCAATGT CAATGCACAA GCTCTGCCAC TCGGAACACA ATGAAACAAG TGACACCTCA
  421 ACCTGAAGAA CAGAAAGAAA GGAAAACCAC AGAAATGCAA AGTCCAATGC AGCCAGTGGA
  481 CCAAGCGAGC CTTCCAGGTC ACTGCAGGGA ACCTCCACCA TGGGAAAATG AAGCCACAGA
  541 GAGAATTTAT CATTTCGTGG TGGGGCAGAT GGTTTATTAT CAGTGCGTCC AGGGATACAG
  601 GGCTCTACAC AGAGGTCCTG CTGAGAGCGT CTGCAAAATG ACCCACGGGA AGACAAGGTG
  661 GACCCAGCCC CAGCTCATAT GCACAGGTGA AATGGAGACC AGTCAGTTTC CAGGTGAAGA
  721 GAAGCCTCAG GCAAGCCCCG AAGGCCGTCC TGAGAGTGAG ACTTCCTGCC TCGTCACAAC
  781 AACAGATTTT CAAATACAGA CAGAAATGGC TGCAACCATG GAGACGTCCA TATTTACAAC
  841 AGAGTACCAG GTAGCAGTGG CCGGCTGTGT TTTCCTGCTG ATCAGCGTCC TCCTCCTGAG
  901 TGGGCTCACC TGGCAGCGGA GACAGAGGAA GAGTAGAAGA ACAATCTAGC CAGCACAATG
  961 GCGGCCGACG CGTCCCGGAG CCATAGATAA AATAAAAAGA TTTTTATTTA GTCTCCAGAA
 1021 AAAGGGGGGA ATGAAAGACC CCCACCTGTA GGTTGGCAGC TAGCTTAAGG TAACGCCATT
 1081 TTTGCAGCAT GAAAAAATAA CATAACTGAG AATAGAGTAG TCAGATTCCA GATCAAGAAC
1141 AGAATGGAAC AAAGCCTGTG AATTATTATT GGGGTC
//
Primer: C-LTR-R RDB15080_A7Epd.seq
>01702_15080_A7Ep_C-LTR_R_B09_06_ABI24.ab1
    1 GCGGCATCTT ACCAGATATC CTGTTTGGCC CATATTCAGC TGTTCCATCT GTTCTTGACC
   61 TTGATCTGAA CTTCTCTATT CTCAGTTATG TATTTTTCCA TGCCTTGCAA AATGGCGTTA
  121 CTTAAGCTAG CTTGCCAAAC CTACAGGTGG GGTCTTTCAT TCCCCCCTTT TTCTGGAGAC
  181 TAAATAAAAT CTTTTATTTT ATCTATGGCT CCGGACGCGT CGGCCGCCAT TGTGCTGGCT
  241 AGATTGTTCT TCTACTCTTC CTCTGTCTCC GCTGCCAGGT GAGCCCACTC AGGAGGAGGA
  301 CGCTGATCAG CAGGAAAACA CAGCCGGCCA CTGCTACCTG GTACTCTGTT GTAAATATGG
  361 ACGTCTCCAT GGTTGCAGCC ATTTCTGTCT GTATTTGAAA ATCTGTTGTT GTGACGAGGC
  421 AGGAAGTCTC ACTCTCAGGA CGGCCTTCGG GGCTTGCCTG AGGCTTCTCT TCACCTGGAA
  481 ACTGACTGGT CTCCATTTCA CCTGTGCATA TGAGCTGGGG CTGGGTCCAC CTTGTCTTCC
  541 CGTGGGTCAT TTTGCAGACG CTCTCAGCAG GACCTCTGTG TAGAGCCCTG TATCCCTGGA
  601 CGCACTGATA ATAAACCATC TGCCCCACCA CGAAATGATA AATTCTCTCT GTGGCTTCAT
  661 TTTCCCATGG TGGAGGTTCC CTGCAGTGAC CTGGAAGGCT CGCTTGGTCC ACTGGCTGCA
  721 TTGGACTTTG CATTTCTGTG GTTTTCCTTT CTTTCTGTTC TTCAGGTTGA GGTGTCACTT
  781 GTTTCATTGT GTTCCGAGTG GCAGAGCTTG TGCATTGACA TTGGTTGTCC CAGGACGAGT
  841 GGCTAGAGTT TCCTGTACAG AGCATATAGA GTGACCCGCT TTTTATTCTG CGGAAACCTC
  901 TCTTGCATTC ACAGTTCAAC ATGGTTCCTT CCTTGTAGGC CATGGCTTTT GAATGTGGCG
961 TGTGGGAATC TCTGGC
//
Primer: pMFG_1 RDB15080_A7Epe.seq
>01702_15080_A7Ep_pMFG-1_A02_01_ABI08.ab1
    1 GCAGGGGGGG GGAGGGGCTG TTAGTAACTC TTGTCATGTC AGGGTCAGGG AAGTTTACAA
   61 GGGGCGGGGG TGCCCCATAT AAGATCTCAT ATGGCCATAT GGGGGCGCCT AGAGAAGGAG
  121 TGAGGGCTGG ATAAAGGGAG GATCGAGGCG GGGTCGAACG AGGAGGTTCA AGGGGGAGAG
  181 ACGGGGCGGA TGGAGGAAGA GGAGGCGGAG GCTTAGGGTG TACAAAGGGC TTGACCCAGG
  241 GAGGGGGGTC AAAAGCCAAG GCTTCCCAGG TCACGATGTA GGGGACCTGG TCTGGGTGTC
  301 CATGCGGGCC AGGTGAAAAG ACCTTGATCT TAACCTGGGT GATGAGGTCT CGGTTAAAGG
  361 TGCCGTCTCG CGGCCATCCG ACGTTAAAGG TTGGCCATTC TGCAGAGCAG AAGGTAACCC
  421 AACGTCTCTT CTTGACATCT ACCGACTGGT TGTGAGCGAT CCGCTCGACA TCTTTCCAGT
  481 GACCTAAGGT CAAACTTAAG GGAGTGGTAA CAGTCTGGCC CGGGCCCATA TTTTCAGACA
  541 AATACAGAAA CACAGTCAGA CAGAGACAAC ACAGAACGAT GCTGCAGCAG ACAAGACGCG
  601 CGGCGCGGCT TCGGTCCCAA ACCGAAAGCA AAAATTCAGA CGGAGGCGGG AACTGTTTTA
  661 GGTTCTCGTC TCCTACCAGA ACCACATATC CCTCCTCTAA GGGGGGTGCA CCAAAGAGTC
  721 CAAAACGATC GGGATTTTTG GACTCAGGTC GGGCCACAAA AACGGCCCCC GAAGTCCCTG
  781 GGACGTCTCC CAGGGTTGCG GCCGGGTGTT CCGAACTCGT CAGTTCCACC ACGGGTCCGC
  841 CAGATACAGA GCTAGTTAGC TAACTAGTAC CGACGCAGGC GCATAAAATC AGTCATAGAC
  901 ACTAGACAAA TCGGACAGAC ACAGATAAGT TGCTGGCCAG CTTACCCTCC CGGGTGGTGG
  961 GGTCGGGTGG TCCCTGGGGC AAGGGGTCTC CCGAATCCCG GGACGAGCCC CCCGAAATGA
 1021 AAGACCCCCG CTGACTGGTA GTTCAATCAC TTCAGAGGAG ACCCCCTTCC CTAGACAGCT
1081 AGACCAACCA AGTCGGAATG GCCAACCGTG CA
//

Please visit Sequencing and PCR primers for primer information.


References

References

reference Wakimoto, H., Efficient retrovirus-mediated cytokine-gene transduction of primary-cultured human glioma cells for tumor vaccination therapy. Jpn. J. cancer Res., 88, 296-305 (1997). PMID 9140115.
user report Tone, Y., Death signalobody: inducing conditional cell death in response to a specific antigen. Hum. Gene Ther. Methods 24 (3):141-150 (2013). PMID 23470213.

2019.03.08

GNP_filter3_RDBDEP_html_190307.pl