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pRx hFAS-i-hCD25

Retroviral vector of hFAS (CD95) and hCD25 (IL2R alpha).

Catalog number RDB01702
Resource name pRx hFAS-i-hCD25
Alternative name CD95 and CD25
Clone info. Retroviral vector of hFAS (CD95) and hCD25 (IL2R alpha) as a surface marker.
Vector backbone pRx (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli)
Gene/insert name Human FAS (CD95) cDNA Human CD25 cDNA
Depositor|Developer Hamada, Hirofumi |
Other clones in our bank

External Database
Human FAS (CD95) Human CD25

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (J. Leukoc. Biol., 60, 181-190 (1996).) and an acknowledgment to the DEPOSITOR are requested.
        Ordering Please visit Information of Request for Distribution.[link] 
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Remarks Remember that you will be working with samples containing infectious virus.
        提供案内 (日本国内) [open/close]

        提供条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献 (J. Leukoc. Biol., 60, 181-190 (1996).) を引用し、謝辞の表明を必要とする。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB01702 pRx hFAS-i-hCD25 DNA solution

        Ordering Information [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Remarks, protocol and/or map (gif) RDB01702.gif

        Original reference

        Further references such as user reports and related articles (go to bottom)

        Featured content


        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15080_A7Epp1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: hFAS-M_R
        Sequence file: RDB15080_A7Epa.seq check
        >01702_15080_A7Ep_hFAS-M_R_B04_04_ABI24.ab1
            1 GTGTATACAG GTAGAGTTAC AAAAAAAGTT TGGTTTACAT CTGCACTTGG TATTCTGGGT
           61 CCGGGTGCAG TTTATTTCCA CTTCTAAGCC ATGTCCTTCA TCACACAATC TACATCTTCT
          121 GCATTTGGAA GAAAAATGGG CTTTGTCTGT GTACTCCTTC CCTTCTTGGC AGGGCACGCA
          181 GTCTGGTTCA TCCCCATTGA CTGTGCAGTC CCTAGCTTTC CTTTCACCTG GAGGACAGGG
          241 CTTATGGCAG AATTGGCCAT CATGATGCAG GCCTTCCAAG TTCTGAGTCT CAACTGTAGT
          301 AACAGTCTTC CTCAATTCCA ATCCCTTGGA GTTGATGTCA GTCACTTGGG CATTAACACT
          361 TTTGGACGAT AATCTAGCAA CAGACGTAAG AACCAGAGGT AGGAGGGTCC AGATGCCCAG
          421 CATGGTGCTC GAGAATTCGA TATCAAGCTT ATCGATACCG TCGACGCCCT ATAGTGAGTC
          481 GTATTACTCT AGAGGATGGT CCACCCCCGG GGTCGGCAGC CTTCACGTGG GCGGCGTGTA
          541 TCCAAGCTGC GATGCCGTCT ACTTTGAGGG CGGTGGGGGT GGTCAGCAGG ACTGTGTAAG
          601 GTCCTTTCCA GCGAGGTTCT AGGTTCTTAG TCTGGTGTCG GCGGACCCAC ACTGTGTCGC
          661 CGACTCGGTA AGGGTGAGGT ACCACCGGTC GGTCCAGTTG TTCTTGGTAG GCTGCCGCCA
          721 GAGGTCTCCA GACTTCGTGC TGGACTAAGT AGAGAGCCTG TAAGTGAGCT TGGAGAGAGG
          781 GGCTGTTAGT AACTCTTGTC ATGTCAGGGT CAGGGAAGTT TACAAGGGGC GGGGGTGCCC
          841 CATATAAGAT CTCATATGGC CATATGGGGG CGCCTAGAGA AGGAGTGAGG GCTGGATAAA
          901 GGGAGGATCG AGGCGGGGTC GAACGAGAGG TTCAAGGGGG AGAGACGGGC GGATGGAGGA
          961 AGAGGAGCGG AGGCTTTAGG GTGTACAAAG GGCTTTGACC CAGGGAGGGG GGTCAAAAGC
         1021 CAAAGCTTCC AGGTCACGAT GTAGGACCTG ATCTGGGTGT CCATGCAGCA GGTGAAAAGA
         1081 CTTGATCTAC CTGGGTGATG AAGTCTCGGT AAGGTGCCGT CTTCGCGGGC CAATTTCGCG
        //
        Primer: hFAS-M_F
        Sequence file: RDB15080_A7Epb.seq check
        >01702_15080_A7Ep_hFAS-M_F_A04_01_ABI24.ab1
            1 TCAAGGGACC AGATACCAGT GCAGATGTAA ACCAAACTTT TTTTGTAACT CTACTGTATG
           61 TGAACACTGT GACCCTTGCA CCAAATGTGA ACATGGAATC ATCAAGGAAT GCACACTCAC
          121 CAGCAACACC AAGTGCAAAG AGGAAGGATC CAGATCTAAC TTGGGGTGGC TTTGTCTTCT
          181 TCTTTTGCCA ATTCCACTAA TTGTTTGGGT GAAGAGAAAG GAAGTACAGA AAACATGCAG
          241 AAAGCACAGA AAGGAAAACC AAGGTTCTCA TGAATCTCCA ACCTTAAATC CTGAAACAGT
          301 GGCAATAAAT TTATCTGATG TTGACTTGAG TAAATATATC ACCACTATTG CTGGAGTCAT
          361 GACACTAAGT CAAGTTAAAG GCTTTGTTCG AAAGAATGGT GTCAATGAAG CCAAAATAGA
          421 TGAGATCAAG AATGACAATG TCCAAGACAC AGCAGAACAG AAAGTTCAAC TGCTTCGTAA
          481 TTGGCATCAA CTTCATGGAA AGAAAGAAGC GTATGACACA TTGATTAAAG ATCTCAAAAA
          541 AGCCAATCTT TGTACTCTTG CAGAGAAAAT TCAGACTATC ATCCTCAAGG ACATTACTAG
          601 TGACTCAGAA AATTCAAACT TCAGAAATGA AATCCAAAGC TTGGTCTAGA ATTCCTGCAG
          661 CCCGGGGGAT CCACTAGTTC TAGAGCGGCC GCAGTATTCT ATAGTGTCAC AATGGATCCT
        721 CTAGAGAATT CCGCCCCCCC CCCCACCCCC CCTCACC
        //
        Primer: IRES-F
        Sequence file: RDB15080_A7Epc.seq check
        >01702_15080_A7Ep_IRES-F_H07_22_ABI24.ab1
            1 AGCAAGTCAT TTCATGTGTT TAGTCGAGGT TAAAAAACGT CTAGGCCCCC CGAACCACGG
           61 GGACGTGGTT TTCCTTTGAA AAACACGATG ATAAGCTTGC CACGACGCGT CTCGACCATT
          121 GTGCTGGAAG ATGGATTCAT ACCTGCTGAT GTGGGGACTG CTCACGTTCA TCATGGTGCC
          181 TGGCTGCCAG GCAGAGCTCT GTGACGATGA CCCGCCAGAG ATCCCACACG CCACATTCAA
          241 AGCCATGGCC TACAAGGAAG GAACCATGTT GAACTGTGAA TGCAAGAGAG GTTTCCGCAG
          301 AATAAAAAGC GGGTCACTCT ATATGCTCTG TACAGGAAAC TCTAGCCACT CGTCCTGGGA
          361 CAACCAATGT CAATGCACAA GCTCTGCCAC TCGGAACACA ATGAAACAAG TGACACCTCA
          421 ACCTGAAGAA CAGAAAGAAA GGAAAACCAC AGAAATGCAA AGTCCAATGC AGCCAGTGGA
          481 CCAAGCGAGC CTTCCAGGTC ACTGCAGGGA ACCTCCACCA TGGGAAAATG AAGCCACAGA
          541 GAGAATTTAT CATTTCGTGG TGGGGCAGAT GGTTTATTAT CAGTGCGTCC AGGGATACAG
          601 GGCTCTACAC AGAGGTCCTG CTGAGAGCGT CTGCAAAATG ACCCACGGGA AGACAAGGTG
          661 GACCCAGCCC CAGCTCATAT GCACAGGTGA AATGGAGACC AGTCAGTTTC CAGGTGAAGA
          721 GAAGCCTCAG GCAAGCCCCG AAGGCCGTCC TGAGAGTGAG ACTTCCTGCC TCGTCACAAC
          781 AACAGATTTT CAAATACAGA CAGAAATGGC TGCAACCATG GAGACGTCCA TATTTACAAC
          841 AGAGTACCAG GTAGCAGTGG CCGGCTGTGT TTTCCTGCTG ATCAGCGTCC TCCTCCTGAG
          901 TGGGCTCACC TGGCAGCGGA GACAGAGGAA GAGTAGAAGA ACAATCTAGC CAGCACAATG
          961 GCGGCCGACG CGTCCCGGAG CCATAGATAA AATAAAAAGA TTTTTATTTA GTCTCCAGAA
         1021 AAAGGGGGGA ATGAAAGACC CCCACCTGTA GGTTGGCAGC TAGCTTAAGG TAACGCCATT
         1081 TTTGCAGCAT GAAAAAATAA CATAACTGAG AATAGAGTAG TCAGATTCCA GATCAAGAAC
        1141 AGAATGGAAC AAAGCCTGTG AATTATTATT GGGGTC
        //
        Primer: C-LTR-R
        Sequence file: RDB15080_A7Epd.seq check
        >01702_15080_A7Ep_C-LTR_R_B09_06_ABI24.ab1
            1 GCGGCATCTT ACCAGATATC CTGTTTGGCC CATATTCAGC TGTTCCATCT GTTCTTGACC
           61 TTGATCTGAA CTTCTCTATT CTCAGTTATG TATTTTTCCA TGCCTTGCAA AATGGCGTTA
          121 CTTAAGCTAG CTTGCCAAAC CTACAGGTGG GGTCTTTCAT TCCCCCCTTT TTCTGGAGAC
          181 TAAATAAAAT CTTTTATTTT ATCTATGGCT CCGGACGCGT CGGCCGCCAT TGTGCTGGCT
          241 AGATTGTTCT TCTACTCTTC CTCTGTCTCC GCTGCCAGGT GAGCCCACTC AGGAGGAGGA
          301 CGCTGATCAG CAGGAAAACA CAGCCGGCCA CTGCTACCTG GTACTCTGTT GTAAATATGG
          361 ACGTCTCCAT GGTTGCAGCC ATTTCTGTCT GTATTTGAAA ATCTGTTGTT GTGACGAGGC
          421 AGGAAGTCTC ACTCTCAGGA CGGCCTTCGG GGCTTGCCTG AGGCTTCTCT TCACCTGGAA
          481 ACTGACTGGT CTCCATTTCA CCTGTGCATA TGAGCTGGGG CTGGGTCCAC CTTGTCTTCC
          541 CGTGGGTCAT TTTGCAGACG CTCTCAGCAG GACCTCTGTG TAGAGCCCTG TATCCCTGGA
          601 CGCACTGATA ATAAACCATC TGCCCCACCA CGAAATGATA AATTCTCTCT GTGGCTTCAT
          661 TTTCCCATGG TGGAGGTTCC CTGCAGTGAC CTGGAAGGCT CGCTTGGTCC ACTGGCTGCA
          721 TTGGACTTTG CATTTCTGTG GTTTTCCTTT CTTTCTGTTC TTCAGGTTGA GGTGTCACTT
          781 GTTTCATTGT GTTCCGAGTG GCAGAGCTTG TGCATTGACA TTGGTTGTCC CAGGACGAGT
          841 GGCTAGAGTT TCCTGTACAG AGCATATAGA GTGACCCGCT TTTTATTCTG CGGAAACCTC
          901 TCTTGCATTC ACAGTTCAAC ATGGTTCCTT CCTTGTAGGC CATGGCTTTT GAATGTGGCG
        961 TGTGGGAATC TCTGGC
        //
        Primer: pMFG_1
        Sequence file: RDB15080_A7Epe.seq check
        >01702_15080_A7Ep_pMFG-1_A02_01_ABI08.ab1
            1 GCAGGGGGGG GGAGGGGCTG TTAGTAACTC TTGTCATGTC AGGGTCAGGG AAGTTTACAA
           61 GGGGCGGGGG TGCCCCATAT AAGATCTCAT ATGGCCATAT GGGGGCGCCT AGAGAAGGAG
          121 TGAGGGCTGG ATAAAGGGAG GATCGAGGCG GGGTCGAACG AGGAGGTTCA AGGGGGAGAG
          181 ACGGGGCGGA TGGAGGAAGA GGAGGCGGAG GCTTAGGGTG TACAAAGGGC TTGACCCAGG
          241 GAGGGGGGTC AAAAGCCAAG GCTTCCCAGG TCACGATGTA GGGGACCTGG TCTGGGTGTC
          301 CATGCGGGCC AGGTGAAAAG ACCTTGATCT TAACCTGGGT GATGAGGTCT CGGTTAAAGG
          361 TGCCGTCTCG CGGCCATCCG ACGTTAAAGG TTGGCCATTC TGCAGAGCAG AAGGTAACCC
          421 AACGTCTCTT CTTGACATCT ACCGACTGGT TGTGAGCGAT CCGCTCGACA TCTTTCCAGT
          481 GACCTAAGGT CAAACTTAAG GGAGTGGTAA CAGTCTGGCC CGGGCCCATA TTTTCAGACA
          541 AATACAGAAA CACAGTCAGA CAGAGACAAC ACAGAACGAT GCTGCAGCAG ACAAGACGCG
          601 CGGCGCGGCT TCGGTCCCAA ACCGAAAGCA AAAATTCAGA CGGAGGCGGG AACTGTTTTA
          661 GGTTCTCGTC TCCTACCAGA ACCACATATC CCTCCTCTAA GGGGGGTGCA CCAAAGAGTC
          721 CAAAACGATC GGGATTTTTG GACTCAGGTC GGGCCACAAA AACGGCCCCC GAAGTCCCTG
          781 GGACGTCTCC CAGGGTTGCG GCCGGGTGTT CCGAACTCGT CAGTTCCACC ACGGGTCCGC
          841 CAGATACAGA GCTAGTTAGC TAACTAGTAC CGACGCAGGC GCATAAAATC AGTCATAGAC
          901 ACTAGACAAA TCGGACAGAC ACAGATAAGT TGCTGGCCAG CTTACCCTCC CGGGTGGTGG
          961 GGTCGGGTGG TCCCTGGGGC AAGGGGTCTC CCGAATCCCG GGACGAGCCC CCCGAAATGA
         1021 AAGACCCCCG CTGACTGGTA GTTCAATCAC TTCAGAGGAG ACCCCCTTCC CTAGACAGCT
        1081 AGACCAACCA AGTCGGAATG GCCAACCGTG CA
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        reference Wakimoto, H., Efficient retrovirus-mediated cytokine-gene transduction of primary-cultured human glioma cells for tumor vaccination therapy. Jpn. J. cancer Res., 88, 296-305 (1997). PMID 9140115.
        user report Tone, Y., Death signalobody: inducing conditional cell death in response to a specific antigen. Hum. Gene Ther. Methods 24 (3):141-150 (2013). PMID 23470213.

        2022.02.09

        GNP_filter3_RDBDEP_html_220112.pl