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pRx nZ ires Neo

Nls (nuclear localization signal), lacZ, ires, neo

Catalog number RDB01699
Resource name pRx nZ ires Neo
Alternative name pRx-nZiN
Clone info. Nls (nuclear localization signal), lacZ, cytomegalovirus immediate early (CMV; IE) enhancer, LTR, internal ribosome entry site (IRES), neomycin
Vector backbone pRx (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name E. coli lacZ (beta-D-galactosidase) Genomic DNA
Depositor|Developer Hamada, Hirofumi |
Other clones in our bank
External Database
E. coli lacZ (beta-D-galactosidase)
          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB01699.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(J. Leukoc. Biol., 60, 181-190 (1996).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Leukoc. Biol., 60, 181-190 (1996).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB01699 pRx nZ ires Neo Under QC test. Please contact us. DNA solution Please contact us.

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pRx nZ ires Neo was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01699).

          Reference section:

          Wakimoto, H., Yoshida, Y., Aoyagi, M., Hirakawa, K., Hamada, H., Efficient retrovirus-mediated cytokine-gene transduction of primary-cultured human glioma cells for tumor vaccination therapy. Jpn. J. cancer Res., 88, 296-305 (1997). PMID 9140115.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content Dnaconda's recommendation EXR0012j (Japanese text)
          Featured content Arranged vector (English text)
          Featured content Empty Backbone (English text)
          Featured content Empty Backbone (Japanese text)

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Wakimoto, H., Efficient retrovirus-mediated cytokine-gene transduction of primary-cultured human glioma cells for tumor vaccination therapy. Jpn. J. cancer Res., 88, 296-305 (1997). PMID 9140115.
          user_report Nagao, T., Proliferation and survival signaling from both Jak2-V617F and Lyn involving GSK3 and mTOR/p70S6K/4EBP1 in PVTL-1 cell line newly established from acute myeloid leukemia transformed from polycythemia vera. PLoS One, 9 (1): e84746 (2014). PMID 24404189.

          2023.05.02

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