RDB01696 - pCM-CD

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icon below as well as clone information before placing your order.

pCM-CD (#RDB01696)

E. coli Cytosine deaminase

Alternative name	CM-cytosine deaminase
Clone info.	CD (cytosine deaminase) under regulation with CM promoter
Comment	NtTA has been removed at the construction of this plasmid.
Vector backbone	pCM-NtTA (Plasmid)
Size of vector backbone	3,875 bp
Selectable markers	Amp^r
Gene/insert name	E. coli Cytosine deaminase (CD) Genomic DNA
Depositor\|Developer	Hamada, Hirofumi \|

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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(J. Leukoc. Biol., 60, 181-190 (1996).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(J. Leukoc. Biol., 60, 181-190 (1996).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Leukoc. Biol., 60, 181-190 (1996).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Leukoc. Biol., 60, 181-190 (1996).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01696	pCM-CD	DNA solution	Please contact us.

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCM-CD was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01696).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13438_A5Fi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB13438_A5Fia.seq
>01696_13438_A5Fi_CMV-Forward_F04_16.ab1 1 TGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTCGT TTAGTGAACC GTCAGATCGC CTGGAGACGC CATCCACGCT GTTTTGACCT 121 CCATAGAAGA CACCGGGACC GATCCAGCCT CCGCGGCCCC GAATTCCGGG GATCCACCAT 181 GTCGAATAAC GCTTTACAAA CAATTATTAA CGCCCGGTTA CCAGGCGAAG AGGGGCTGTG 241 GCAGATTCAT CTGCAGGACG GAAAAATCAG CGCCATTGAT GCGCAATCCG GCGTGATGCC 301 CATAACTGAA AACAGCCTGG ATGCCGAACA AGGTTTAGTT ATACCGCCGT TTGTGGAGCC 361 ACATATTCAC CTGGACACCA CGCAAACCGC CGGACAACCG AACTGGAATC AGTCCGGCAC 421 GCTGTTTGAA GGCATTGAAC GCTGGGCCGA GCGCAAAGCG TTATTAACCC ATGACGATGT 481 GAAACAACGC GCATGGCAAA CGCTGAAATG GCAGATTGCC AACGGCATTC AGCATGTGCG 541 TACCCATGTC GATGTTTCGG ATGCAACGCT AACTGCGCTG AAAGCAATGC TGGAAGTGAA 601 GCAGGAAGTC GCGCCGTGGA TTGATCTGCA AATCGTCGCC TTCCCTCAGG AAGGGATTTT 661 GTCGTATCCC AACGGTGAAG CGTTGCTGGA AGAGGCGTTA CGCTTAGGGG CAGATGTAGT 721 GGGGGCGATT CCGCATTTTG AATTTACCCG TGAATACGGC GTGGAGTCGC TGCATAAAAC 781 CTTCGCCCTG GCGCAAAAAT ACGACCGTCT CATCGACGTT CACTGTGATG AGATCGATGA 841 CGAGCAGTCG CGCTTTGTCG AAACCGTTGC TGCCCTGGCG CACCATGAAG GCATGGGCGC 901 GCGAGTCACC GCCAGCCACA CCACGGCAAT GCACTCCTAT AACGGGGCGT ATACCTCACG 961 CCTGTTCCGC TTGCTGAAAA TGTCCGGTAT TAACTTTGTC GCCCAACCCG CTGGTCATAT 1021 TCATCTGCAA GGACGTTTCG ATACGTATCC CAAAACGTCG CGGCATCACG CCGCGTTAAG 1081 AAGATGCTGG AGTCCGGGCA TTAACGGTCT GCCTTGGTCA CGGAATGGAT GGTCTTTCGA 1141 TCCGATGGTA TCCCGCTGGG AACCGGGCCA AT //
Primer: pAxCALNL_F1 Sequence file: RDB13438_A5Fib.seq
>01696_13438_A5Fi_pAxCALNL_F1_F09_18.ab1 1 ATATCTATCT TATCATGTCT GGATCTCAGT GGTATTTGTG AGCCAGGGCA TTGGCCACAC 61 CAGCCACCAC CTTCTGATAG GCAGCCTGCA CCTGAGGAGT GAATTCCTAC GAGTTCAGTG 121 CTCTACGTAG GCCGGATAAG GCGTTCACGC CGCATCCGGC ATAAACTAAG CTCGCTGTAA 181 CCCAGTCGTT CAACGTTTGT AATCGATGGC TTCTGGCTGC TCCAGATATA CGGTGGTTTG 241 TGCCGGTTGT GTGCTGGCAA TCACCTTGCC GCCACGTACC GAATAACGTA CCGGAACCTG 301 ACGGCGCAGC GCATCAAACC CATTTTCAGC CGGCAGGATA ATCAGGTTGG CGCTGTTTCC 361 GGCGGCAATG CCGTAATCCT GCAAATTCAA CGTCCTTGCG CTGTGGTGGG TGATTAAATT 421 CAGGCCATCG TTAATCTGCC CGTAGCCCAT CAACTGGCAA ACATGCAGCC CCATATGCAG 481 CACTTGCAGC ATATTCGCCG TTCCCAGCGG ATACCACGGA TCGAAGACAT CATCGTGACC 541 AAAGCAGACG TTAATGCCGG ACTCCAGCAT CTCTTTAACG CGCGTGATGC CGCGACGTTT 601 TGGATACGTA TCGAAACGTC CTTGCAGATG AATATTGACC AGCGGGTTGG CGACAAAGTT 661 AATACCGGAC ATTTTCAGCA AGCGGAACAG GCGTGAGGTA TACGCCCCGT TATAGGAGTG 721 CATTGCCGTG GTGTGGCTGG CGGTGACTCG CGCGCCCATG CCTTCATGGT GCGCCAGGGC 781 AGCAACGGTT TCGACAAAGC GCGACTGCTC GTCATCGATC TCATCACAGT GAACGTCGAT 841 GAGACGGTCG TATTTTTGCG CCAGGGCGAA GGTTTTATGC AGCGACTCCA CGCCGTATTC 901 ACGGGTAAAT TCAAAATGCG GAATCGCCCC CACTACATCT GCCCCTAAGC GTAACGCCTC 961 TTCCAGCAAC GCTTCACCGT TGGGATACGA CAAAATCCCT TCCTGAGGGA AGGCGACGAT 1021 TTGCAGATCA ATCCACGGCG CGACTTCCTG CTTCACTTCC AGCAATGCTT TCAGCGCAGT 1081 TAGCGTTGCA TCCGAAACAT CGACATGGGT ACGCACATGC CTGATGCGTA GCATCTGGCC 1141 CATTTCAGCG ATTGCCAATG GCGCGGTGGT TTCACACACT CGCGCTCCAA TTG //
Primer: SV40pA3'Element Reverse Sequence file: RDB13438_A5Fic.seq
>01696_13438_A5Fi_SV40pA3'ElementReverse_D12_12.ab1 1 GTGTCAGGGG CGGAGCCTAT GGAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 61 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 121 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 181 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 241 GGCCGATTCA TTAATGCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA 301 ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA 361 TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGCCAAGCTT GGTCGAGCTG 421 GATACTTCCC GTCCGCCAGG GGGACATGCC GGCGATGCTG AAGGTCGCGC GCATTCCCGA 481 TGAAGAGGCC GGTTACCGCC TGTTGACCTG GTGGGACGGG CAGGGCGCCG CCCGAGTCTT 541 CGCCTCGGCG GCGGGCGCTC TGCTCATGGA GCGCGCGTCC GGGGCCGGGG ACCTTGCACA 601 GATAGCGTGG TCCGGCCAGG ACGACGAGGC TTGCAGGATC ATAATCAGCC ATACCACATT 661 TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA 721 AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG 781 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT 841 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGATCTCA GTGGTATTTG TGAGCCAGGG 901 CATTGGCCAC ACCAGCCACC ACCTTCTGAT AGGCAGCCTG CACCTGAGGA GTGAATTCCT 961 ACGAGTTCAG TGCTCTACGT AGGCCGGATA AAGGCGTTCA CGCCGCATCC GGGCATAAAC 1021 TAAGCTCGCT GTAACCCAGT CGTTTCAACG TTTGTAATCG ATGGCTTCTG GCTGCTCCAG 1081 ATATACGGTG ATTGTGCCGC TGTGTGCTGG CAATCACGTG GCGGCACGTA CGATACGTTA 1141 CCCGGAACCC TGACGGCGCA GGCGCCAATT CAAACCCCAA TT //