Resource data sheet
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pCM-CD

E. coli Cytosine deaminase

Catalog number RDB01696
Resource name pCM-CD
Alternative name CM-cytosine deaminase
Clone info. CD (cytosine deaminase) under regulation with CM promoter
NtTA has been removed at the construction of this plasmid.
Vector backbone pCM-NtTA (Plasmid)
Size of vector backbone 3,875 bp
Selectable markers Amp^r
Gene/insert name E. coli Cytosine deaminase (CD) Genomic DNA
Depositor Hamada, Hirofumi |
 
Other clones in our bank E. coli codA (KEGG orthology K01485) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(J. Leukoc. Biol., 60, 181-190 (1996).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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RDB01696 pCM-CD DNA solution Please contact us.

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References and tips

Electronic file

Electronic file Map RDB01696.gif from Depositor

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References

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13438_A5Fi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13438_A5Fia.seq
>01696_13438_A5Fi_CMV-Forward_F04_16.ab1
    1 TGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCGT TTAGTGAACC GTCAGATCGC CTGGAGACGC CATCCACGCT GTTTTGACCT
  121 CCATAGAAGA CACCGGGACC GATCCAGCCT CCGCGGCCCC GAATTCCGGG GATCCACCAT
  181 GTCGAATAAC GCTTTACAAA CAATTATTAA CGCCCGGTTA CCAGGCGAAG AGGGGCTGTG
  241 GCAGATTCAT CTGCAGGACG GAAAAATCAG CGCCATTGAT GCGCAATCCG GCGTGATGCC
  301 CATAACTGAA AACAGCCTGG ATGCCGAACA AGGTTTAGTT ATACCGCCGT TTGTGGAGCC
  361 ACATATTCAC CTGGACACCA CGCAAACCGC CGGACAACCG AACTGGAATC AGTCCGGCAC
  421 GCTGTTTGAA GGCATTGAAC GCTGGGCCGA GCGCAAAGCG TTATTAACCC ATGACGATGT
  481 GAAACAACGC GCATGGCAAA CGCTGAAATG GCAGATTGCC AACGGCATTC AGCATGTGCG
  541 TACCCATGTC GATGTTTCGG ATGCAACGCT AACTGCGCTG AAAGCAATGC TGGAAGTGAA
  601 GCAGGAAGTC GCGCCGTGGA TTGATCTGCA AATCGTCGCC TTCCCTCAGG AAGGGATTTT
  661 GTCGTATCCC AACGGTGAAG CGTTGCTGGA AGAGGCGTTA CGCTTAGGGG CAGATGTAGT
  721 GGGGGCGATT CCGCATTTTG AATTTACCCG TGAATACGGC GTGGAGTCGC TGCATAAAAC
  781 CTTCGCCCTG GCGCAAAAAT ACGACCGTCT CATCGACGTT CACTGTGATG AGATCGATGA
  841 CGAGCAGTCG CGCTTTGTCG AAACCGTTGC TGCCCTGGCG CACCATGAAG GCATGGGCGC
  901 GCGAGTCACC GCCAGCCACA CCACGGCAAT GCACTCCTAT AACGGGGCGT ATACCTCACG
  961 CCTGTTCCGC TTGCTGAAAA TGTCCGGTAT TAACTTTGTC GCCCAACCCG CTGGTCATAT
 1021 TCATCTGCAA GGACGTTTCG ATACGTATCC CAAAACGTCG CGGCATCACG CCGCGTTAAG
 1081 AAGATGCTGG AGTCCGGGCA TTAACGGTCT GCCTTGGTCA CGGAATGGAT GGTCTTTCGA
1141 TCCGATGGTA TCCCGCTGGG AACCGGGCCA AT
//
Primer: pAxCALNL_F1 RDB13438_A5Fib.seq
>01696_13438_A5Fi_pAxCALNL_F1_F09_18.ab1
    1 ATATCTATCT TATCATGTCT GGATCTCAGT GGTATTTGTG AGCCAGGGCA TTGGCCACAC
   61 CAGCCACCAC CTTCTGATAG GCAGCCTGCA CCTGAGGAGT GAATTCCTAC GAGTTCAGTG
  121 CTCTACGTAG GCCGGATAAG GCGTTCACGC CGCATCCGGC ATAAACTAAG CTCGCTGTAA
  181 CCCAGTCGTT CAACGTTTGT AATCGATGGC TTCTGGCTGC TCCAGATATA CGGTGGTTTG
  241 TGCCGGTTGT GTGCTGGCAA TCACCTTGCC GCCACGTACC GAATAACGTA CCGGAACCTG
  301 ACGGCGCAGC GCATCAAACC CATTTTCAGC CGGCAGGATA ATCAGGTTGG CGCTGTTTCC
  361 GGCGGCAATG CCGTAATCCT GCAAATTCAA CGTCCTTGCG CTGTGGTGGG TGATTAAATT
  421 CAGGCCATCG TTAATCTGCC CGTAGCCCAT CAACTGGCAA ACATGCAGCC CCATATGCAG
  481 CACTTGCAGC ATATTCGCCG TTCCCAGCGG ATACCACGGA TCGAAGACAT CATCGTGACC
  541 AAAGCAGACG TTAATGCCGG ACTCCAGCAT CTCTTTAACG CGCGTGATGC CGCGACGTTT
  601 TGGATACGTA TCGAAACGTC CTTGCAGATG AATATTGACC AGCGGGTTGG CGACAAAGTT
  661 AATACCGGAC ATTTTCAGCA AGCGGAACAG GCGTGAGGTA TACGCCCCGT TATAGGAGTG
  721 CATTGCCGTG GTGTGGCTGG CGGTGACTCG CGCGCCCATG CCTTCATGGT GCGCCAGGGC
  781 AGCAACGGTT TCGACAAAGC GCGACTGCTC GTCATCGATC TCATCACAGT GAACGTCGAT
  841 GAGACGGTCG TATTTTTGCG CCAGGGCGAA GGTTTTATGC AGCGACTCCA CGCCGTATTC
  901 ACGGGTAAAT TCAAAATGCG GAATCGCCCC CACTACATCT GCCCCTAAGC GTAACGCCTC
  961 TTCCAGCAAC GCTTCACCGT TGGGATACGA CAAAATCCCT TCCTGAGGGA AGGCGACGAT
 1021 TTGCAGATCA ATCCACGGCG CGACTTCCTG CTTCACTTCC AGCAATGCTT TCAGCGCAGT
 1081 TAGCGTTGCA TCCGAAACAT CGACATGGGT ACGCACATGC CTGATGCGTA GCATCTGGCC
1141 CATTTCAGCG ATTGCCAATG GCGCGGTGGT TTCACACACT CGCGCTCCAA TTG
//
Primer: SV40pA3'Element Reverse RDB13438_A5Fic.seq
>01696_13438_A5Fi_SV40pA3'ElementReverse_D12_12.ab1
    1 GTGTCAGGGG CGGAGCCTAT GGAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT
   61 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC
  121 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG
  181 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT
  241 GGCCGATTCA TTAATGCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA
  301 ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA
  361 TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGCCAAGCTT GGTCGAGCTG
  421 GATACTTCCC GTCCGCCAGG GGGACATGCC GGCGATGCTG AAGGTCGCGC GCATTCCCGA
  481 TGAAGAGGCC GGTTACCGCC TGTTGACCTG GTGGGACGGG CAGGGCGCCG CCCGAGTCTT
  541 CGCCTCGGCG GCGGGCGCTC TGCTCATGGA GCGCGCGTCC GGGGCCGGGG ACCTTGCACA
  601 GATAGCGTGG TCCGGCCAGG ACGACGAGGC TTGCAGGATC ATAATCAGCC ATACCACATT
  661 TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA
  721 AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG
  781 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT
  841 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGATCTCA GTGGTATTTG TGAGCCAGGG
  901 CATTGGCCAC ACCAGCCACC ACCTTCTGAT AGGCAGCCTG CACCTGAGGA GTGAATTCCT
  961 ACGAGTTCAG TGCTCTACGT AGGCCGGATA AAGGCGTTCA CGCCGCATCC GGGCATAAAC
 1021 TAAGCTCGCT GTAACCCAGT CGTTTCAACG TTTGTAATCG ATGGCTTCTG GCTGCTCCAG
 1081 ATATACGGTG ATTGTGCCGC TGTGTGCTGG CAATCACGTG GCGGCACGTA CGATACGTTA
1141 CCCGGAACCC TGACGGCGCA GGCGCCAATT CAAACCCCAA TT
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

GNP_filter3_RDBDEP_html_181012.pl