Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCM-TK

CM-HSV-TK genomic DNA

Catalog number RDB01692
Resource name pCM-TK
Alternative name CM-TK
Clone info. HSV TK (thymidine kinase) under regulation of CMV promoter.
Comment CMV promoter was from pUHD 15-1. PhCMV*-1 and SV40 poly(A) sequences have been removed from pUHD 10-3 vector.
Vector backbone pUHD10-3 (Plasmid)
Size of vector backbone 6,646 bp
Selectable markers Amp^r
Gene/insert name HSV thymidine kinase Genomic DNA
Depositor|Developer Hamada, Hirofumi |
Other clones in our bank

External Database
HSV thymidine kinase

          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB01692.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature (J. Leukoc. Biol., 60, 181-190 (1996)) designated by the DEPOSITOR and an acknowledgment to the DEPOSITOR are requested.
          Ordering
          Order form [Credit Card Payment]  [Bank Transfer Payment]
          MTA, for use for not-for-profit academic purpose [Word]
          Please visit Information of Request for Distribution.[link] 
          提供案内 (日本国内) [open/close]

          提供条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献 (J. Leukoc. Biol., 60, 181-190 (1996)) を引用し、謝辞の表明を必要とする。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA, 非営利学術目的用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB01692 pCM-TK DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCM-TK was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01692).

          Reference section:

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content

          Featured content Suicide gene therapy (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15400_A7Iep1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB15400_A7Iea.seq check
          >01692_15400_A7Ie_CMV_Forward_A01_01_ABI08.ab1
              1 ACGTATCACT CCGCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
             61 TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA CGCTGTTTTG
            121 ACCTCCATAG AAGACACCGG GACCGATCCA GCCTCCGCGG CCCCGAATTC CGGGGATCGA
            181 TCTTGGTGGC GTGAAACTCC CGCACCTCTT CGGCAAGCGC CTTGTAGAAG CGCGTATGGC
            241 TTCGTACCCC TGCCATCAAC ACGCGTCTGC GTTCGACCAG GCTGCGCGTT CTCGCGGCCA
            301 TAGCAACCGA CGTACGGCGT TGCGCCCTCG CCGGCAGCAA GAAGCCACGG AAGTCCGCCT
            361 GGAGCAGAAA ATGCCCACGC TACTGCGGGT TTATATAGAC GGTCCTCACG GGATGGGGAA
            421 AACCACCACC ACGCAACTGC TGGTGGCCCT GGGTTCGCGC GACGATATCG TCTACGTACC
            481 CGAGCCGATG ACTTACTGGC AGGTGCTGGG GGCTTCCGAG ACAATCGCGA ACATCTACAC
            541 CACACAACAC CGCCTCGACC AGGGTGAGAT ATCGGCCGGG GACGCGGCGG TGGTAATGAC
            601 AAGCGCCCAG ATAACAATGG GCATGCCTTA TGCCGTGACC GACGCCGTTC TGGCTCCTCA
            661 TATCGGGGGG GAGGCTGGGA GCTCACATGC CCCGCCCCCG GCCCTCACCC TCATCTTCGA
            721 CCGCCATCCC ATCGCCGCCC TCCTGTGCTA CCCGGCCGCG CGATACCTTA TGGGCAGCAT
            781 GACCCCCCAG GCCGTGCTGG CGTTCGTGGC CCTCATCCCG CCGACCTTGC CCGGCACAAA
            841 CATCGTGTTG GGGGCCCTTC CGGAGGACAG ACACATCGAC CGCCTGGCCA AACGCCAGCG
            901 CCCCGGCGAG CGGCTTGACC TGGCTATGCT GGCCGCGATT CGCCGCGTTT ACGGGCTGCT
            961 TGCCAATACG GTGCGGTATC TGCAGGGCGG CGGGTCGTGG CGGGAGGATT GGGGACAGCT
           1021 TTCGGGACGG CCGTGCCGCC CCAGGGTGCC GAGCCCCAGA GCAACGCCGG CTCACGACCC
           1081 CATATTCGGG GAACACGTTA ATTAACCTTG AATCCGGGCC CCGAAGTTGC TGGGTCCCCC
          1141 CCAAACG
          //
          Primer: HSV TK pA_R
          Sequence file: RDB15400_A7Ieb.seq check
          >01692_15400_A7Ie_HSV_TK_pA_R_C11_08_ABI24.ab1
              1 AGGGGACTTC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCATC TCCCGGGCAA
             61 ACGTGCGCGC CAGGTCGCAG ATCGTCGGTA TGGAGCCGGG GGTGGTGACG TGGGTCTGGA
            121 CCATCCCGGA GGTAAGTTGC AGCAGGGCGT CCCGGCAGCC GGCGGGCGAT TGGTCGTAAT
            181 CCAGGATAAA GACGTGCATG GGACGGAGGC GTTTGGCCAA GACGTCCAAG GCCCAGGCAA
            241 ACACGTTGTA CAGGTCGCCG TTGGGGGCCA GCAACTCGGG GGCCCGAAAC AGGGTAAATA
            301 ACGTGTCCCC GATATGGGGT CGTGGGCCCG CGTTGCTCTG GGGCTCGGCA CCCTGGGGCG
            361 GCACGGCCGT CCCCGAAAGC TGTCCCCAAT CCTCCCGCCA CGACCCGCCG CCCTGCAGAT
            421 ACCGCACCGT ATTGGCAAGC AGCCCGTAAA CGCGGCGAAT CGCGGCCAGC ATAGCCAGGT
            481 CAAGCCGCTC GCCGGGGCGC TGGCGTTTGG CCAGGCGGTC GATGTGTCTG TCCTCCGGAA
            541 GGGCCCCCAA CACGATGTTT GTGCCGGGCA AGGTCGGCGG GATGAGGGCC ACGAACGCCA
            601 GCACGGCCTG GGGGGTCATG CTGCCCATAA GGTATCGCGC GGCCGGGTAG CACAGGAGGG
            661 CGGCGATGGG ATGGCGGTCG AAGATGAGGG TGAGGGCCGG GGGCGGGGCA TGTGAGCTCC
            721 CAGCCTCCCC CCCGATATGA GGAGCCAGAA CGGCGTCGGT CACGGCATAA GGCATGCCCA
            781 TTGTTATCTG GGCGCTTGTC ATTACCACCG CCGCGTCCCC GGCCGATATC TCACCCTGGT
            841 CGAGGCGGTG TTGTGTGGTG TAGATGTTCG CGATTGTCTC GGAAGCCCCC AGCACCTGCC
            901 AGTAAGTCAT CGGCTCGGGT ACGTAGACGA TATCGTCGCG CGAACCCAGG GCCACCAGCA
            961 GTTGCGTGGT GGTGGTTTTC CCCATCCCGT GAGGACCGTC TATATAAACC CGCAGTAGCG
           1021 TGGGCATTTT TCTGCTCCAG GCGGACTTCC GTGGCTTCTT GCTGCCCGGC GAGGGCGCAC
           1081 GCGTACGTCG GTTTGCTATG ACGCGAGACG CGCAGCTTGA TTCGAAACGC AGACGCCGAT
          1141 GTTTGGAATT GC
          //
          Primer: PGKpA_R
          Sequence file: RDB15400_A7Iec.seq check
          >01692_15400_A7Ie_PGKpA_R_B01_02_ABI08.ab1
              1 GGGCCTAACT TCTTACAAGT ATGACAGGAA AACTTCCATT TTAGTGGACA TCTTTATTGT
             61 TTAATAGATC ATCAATTTCT GCAGGTCCTG GCCGGGGTCC CGCGGAAACT CGGCCGTGGT
            121 GACCAATACA AAACAAAAGC GCTCCTCGTA CCAGCGAAGA AGGGGCAGAG ATGCCGTAGT
            181 CAGGTTTAGT TCGTCCGGCG GCGCCAGAAA TCCGCGCGGT GGTTTTTGGG GGTCGGGGGT
            241 GTTTGGCAGC CACAGACGCC CGGTGTTCGT GTCGCGCCAG TACATGCGGT CCATGCCCAG
            301 GCCATCCAAA AACCATGGGT CTGTCTGCTC AGTCCAGTCG TGGACCTGAC CCCACGCAAC
            361 GCCCAAAATA ATAACCCCCA CGAACCATAA ACCATTCCCC ATGGGGGACC CCGTCCCTAA
            421 CCCACGGGGC CCGTGGCTAT GGCAGGGCTT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG
            481 CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC
            541 CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA
            601 TGAACAAACG ACCCAACACC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG
            661 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCATC TCCCGGGCAA
            721 ACGTGCGCGC CAGGTCGCAG ATCGTCGGTA TGGAGCCGGG GGTGGTGACG TGGGTCTGGA
            781 CCATCCCGGA GGTAAGTTGC AGCAGGGCGT CCCGGCAGCC GGCGGGCGAT TGGTCGTAAT
            841 CCAGGATAAA GACGTGCATG GGGACGGAGG CGTTTGGCCA AGACGTCCAA GGCCCAGGCA
            901 AACACGTTGT ACAGGTCGCC GTTGGGGGCC AGCAACTCGG GGGGCCCGAA ACAGGGTAAA
            961 TAACGTGTCC CCGATATGGG GTCGTGGGCC CGCGTTGCTC TGGGGCTTCG GCACCCTGGG
           1021 GGCGGGCACG GCCGTTCCCC CGAAAAGCTG TCCCAATCCT CCCGGCCACG GACCCGCCCG
           1081 CCTCCTGCCA GGATACCGGC ACCGTAATGG GCAAGGCCAG CCCGTTAACG CGGCGAAATC
          1141 CGCCGCCAGC CAATAGACTA AGGTTCAAAG CTCAGTCCTT CGCTGT
          //
          Primer: SV40pA3'Element Reverse
          Sequence file: RDB15400_A7Ied.seq check
          >01692_15400_A7Ie_SV40pA3'ElementReverse_B11_05_ABI24.ab1
              1 GACTCAAGGG GGGGCGGACC TATGGAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG
             61 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT
            121 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC
            181 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG
            241 CGTTGGCCGA TTCATTAATG CAGCTGAACG GTCTGGTTAT AGGTACATTG AGCAACTGAC
            301 TGAAATGCCT CAAAATGTTC TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA
            361 GTGATTTTTT TCTCCATTTT AGCTTCCTTA GCTCCTGAAA ATCTCGCCAA GCTTCTGATG
            421 GAATTAGAAC TTGGCAAAAC AATACTGAGA ATGAAGTGTA TGTGGAACAG AGGCTGCTGA
            481 TCTCGTTCTT CAGGCTATGA AACTGACACA TTTGGAAACC ACAGTACTTA GAACCACAAA
            541 GTGGGAATCA AGAGAAAAAC AATGATCCCA CGAGAGATCT ATAGATCTAT AGATCATGAG
            601 TGGGAGGAAT GAGCTGGCCC TTAATTTGGT TTTGCTTGTT TAAATTATGA TATCCAACTA
            661 TGAAACATTA TCATAAAGCA ATAGTAAAGA GCCTTCAGTA AAGAGCAGGC ATTTATCTAA
            721 TCCCACCCCA CCCCCACCCC CGTAGCTCCA ATCCTTCCAT TCAAAATGTA GGTACTCTGT
            781 TCTCACCCTT CTTAACAAAG TATGACAGGA AAAACTTCCA TTTTAGTGGA CATCTTTATT
            841 GTTTAATAGA TCATCAATTT CTGCAGGTCC TGGCCGGGGT CCCGCGGAAA CTCGGCCGTG
            901 GTGACCAATA CAAAACAAAA GCGCTCCTCG TACCAGCGAA GAAAGGGCAG AGATGCCGTA
            961 GTCAGGTTTA GTTCGTCCGG CGGCGCCAGA AATCCGCGCG GTGGGTTTTT GGGGGGTCGG
           1021 GGGGGTGTTT GGCAGCCACA GACGCCCGGG TGTTCGTGTC GCGCCAGTAC ATGCGGGTCC
           1081 AATGCCCAGG CCCATCCAAA ATCATGGGTC CTGTCCTGCT TCAGGTTCCA GTCGGTGGAC
          1141 CTGGACCCCC CACGGCCA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Kawasaki M., Inducible liver injury in the transgenic rat by expressing liver-specific suicide gene. Biochem. Biophys. Res. Commun., 311, 920-928 (2003). PMID 14623269. [link to RRC of NBRP]
          user_report Mizuguchi H., Enhanced antitumor effect and reduced vector dissemination with fiber-modified adenovirus vectors expressing herpes simplex virus thymidine kinase. Cancer Gene Ther., 9, 236-242 (2002). PMID 11896439. [link to RRC of NBRP]

          2024.01.19

          GNP_filter3_RDBDEP_html_240118.pl