Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCM-TK (#RDB01692)

CM-HSV-TK genomic DNA

Alternative name CM-TK
Clone info. HSV TK (thymidine kinase) under regulation of CMV promoter.
Comment CMV promoter was from pUHD 15-1. PhCMV*-1 and SV40 poly(A) sequences have been removed from pUHD 10-3 vector.
Vector backbone pUHD10-3 (Plasmid)
Size of vector backbone 6,646 bp
Selectable markers Amp^r
Gene/insert name HSV thymidine kinase Genomic DNA
Depositor|Developer Hamada, Hirofumi |
 
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature (J. Leukoc. Biol., 60, 181-190 (1996)) designated by the DEPOSITOR and an acknowledgment to the DEPOSITOR are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献 (J. Leukoc. Biol., 60, 181-190 (1996)) を引用し、謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01692 pCM-TK DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCM-TK was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01692).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15400_A7Iep1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB15400_A7Iea.seq check
>01692_15400_A7Ie_CMV_Forward_A01_01_ABI08.ab1
    1 ACGTATCACT CCGCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA CGCTGTTTTG
  121 ACCTCCATAG AAGACACCGG GACCGATCCA GCCTCCGCGG CCCCGAATTC CGGGGATCGA
  181 TCTTGGTGGC GTGAAACTCC CGCACCTCTT CGGCAAGCGC CTTGTAGAAG CGCGTATGGC
  241 TTCGTACCCC TGCCATCAAC ACGCGTCTGC GTTCGACCAG GCTGCGCGTT CTCGCGGCCA
  301 TAGCAACCGA CGTACGGCGT TGCGCCCTCG CCGGCAGCAA GAAGCCACGG AAGTCCGCCT
  361 GGAGCAGAAA ATGCCCACGC TACTGCGGGT TTATATAGAC GGTCCTCACG GGATGGGGAA
  421 AACCACCACC ACGCAACTGC TGGTGGCCCT GGGTTCGCGC GACGATATCG TCTACGTACC
  481 CGAGCCGATG ACTTACTGGC AGGTGCTGGG GGCTTCCGAG ACAATCGCGA ACATCTACAC
  541 CACACAACAC CGCCTCGACC AGGGTGAGAT ATCGGCCGGG GACGCGGCGG TGGTAATGAC
  601 AAGCGCCCAG ATAACAATGG GCATGCCTTA TGCCGTGACC GACGCCGTTC TGGCTCCTCA
  661 TATCGGGGGG GAGGCTGGGA GCTCACATGC CCCGCCCCCG GCCCTCACCC TCATCTTCGA
  721 CCGCCATCCC ATCGCCGCCC TCCTGTGCTA CCCGGCCGCG CGATACCTTA TGGGCAGCAT
  781 GACCCCCCAG GCCGTGCTGG CGTTCGTGGC CCTCATCCCG CCGACCTTGC CCGGCACAAA
  841 CATCGTGTTG GGGGCCCTTC CGGAGGACAG ACACATCGAC CGCCTGGCCA AACGCCAGCG
  901 CCCCGGCGAG CGGCTTGACC TGGCTATGCT GGCCGCGATT CGCCGCGTTT ACGGGCTGCT
  961 TGCCAATACG GTGCGGTATC TGCAGGGCGG CGGGTCGTGG CGGGAGGATT GGGGACAGCT
 1021 TTCGGGACGG CCGTGCCGCC CCAGGGTGCC GAGCCCCAGA GCAACGCCGG CTCACGACCC
 1081 CATATTCGGG GAACACGTTA ATTAACCTTG AATCCGGGCC CCGAAGTTGC TGGGTCCCCC
1141 CCAAACG
//
Primer: HSV TK pA_R
Sequence file: RDB15400_A7Ieb.seq check
>01692_15400_A7Ie_HSV_TK_pA_R_C11_08_ABI24.ab1
    1 AGGGGACTTC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCATC TCCCGGGCAA
   61 ACGTGCGCGC CAGGTCGCAG ATCGTCGGTA TGGAGCCGGG GGTGGTGACG TGGGTCTGGA
  121 CCATCCCGGA GGTAAGTTGC AGCAGGGCGT CCCGGCAGCC GGCGGGCGAT TGGTCGTAAT
  181 CCAGGATAAA GACGTGCATG GGACGGAGGC GTTTGGCCAA GACGTCCAAG GCCCAGGCAA
  241 ACACGTTGTA CAGGTCGCCG TTGGGGGCCA GCAACTCGGG GGCCCGAAAC AGGGTAAATA
  301 ACGTGTCCCC GATATGGGGT CGTGGGCCCG CGTTGCTCTG GGGCTCGGCA CCCTGGGGCG
  361 GCACGGCCGT CCCCGAAAGC TGTCCCCAAT CCTCCCGCCA CGACCCGCCG CCCTGCAGAT
  421 ACCGCACCGT ATTGGCAAGC AGCCCGTAAA CGCGGCGAAT CGCGGCCAGC ATAGCCAGGT
  481 CAAGCCGCTC GCCGGGGCGC TGGCGTTTGG CCAGGCGGTC GATGTGTCTG TCCTCCGGAA
  541 GGGCCCCCAA CACGATGTTT GTGCCGGGCA AGGTCGGCGG GATGAGGGCC ACGAACGCCA
  601 GCACGGCCTG GGGGGTCATG CTGCCCATAA GGTATCGCGC GGCCGGGTAG CACAGGAGGG
  661 CGGCGATGGG ATGGCGGTCG AAGATGAGGG TGAGGGCCGG GGGCGGGGCA TGTGAGCTCC
  721 CAGCCTCCCC CCCGATATGA GGAGCCAGAA CGGCGTCGGT CACGGCATAA GGCATGCCCA
  781 TTGTTATCTG GGCGCTTGTC ATTACCACCG CCGCGTCCCC GGCCGATATC TCACCCTGGT
  841 CGAGGCGGTG TTGTGTGGTG TAGATGTTCG CGATTGTCTC GGAAGCCCCC AGCACCTGCC
  901 AGTAAGTCAT CGGCTCGGGT ACGTAGACGA TATCGTCGCG CGAACCCAGG GCCACCAGCA
  961 GTTGCGTGGT GGTGGTTTTC CCCATCCCGT GAGGACCGTC TATATAAACC CGCAGTAGCG
 1021 TGGGCATTTT TCTGCTCCAG GCGGACTTCC GTGGCTTCTT GCTGCCCGGC GAGGGCGCAC
 1081 GCGTACGTCG GTTTGCTATG ACGCGAGACG CGCAGCTTGA TTCGAAACGC AGACGCCGAT
1141 GTTTGGAATT GC
//
Primer: PGKpA_R
Sequence file: RDB15400_A7Iec.seq check
>01692_15400_A7Ie_PGKpA_R_B01_02_ABI08.ab1
    1 GGGCCTAACT TCTTACAAGT ATGACAGGAA AACTTCCATT TTAGTGGACA TCTTTATTGT
   61 TTAATAGATC ATCAATTTCT GCAGGTCCTG GCCGGGGTCC CGCGGAAACT CGGCCGTGGT
  121 GACCAATACA AAACAAAAGC GCTCCTCGTA CCAGCGAAGA AGGGGCAGAG ATGCCGTAGT
  181 CAGGTTTAGT TCGTCCGGCG GCGCCAGAAA TCCGCGCGGT GGTTTTTGGG GGTCGGGGGT
  241 GTTTGGCAGC CACAGACGCC CGGTGTTCGT GTCGCGCCAG TACATGCGGT CCATGCCCAG
  301 GCCATCCAAA AACCATGGGT CTGTCTGCTC AGTCCAGTCG TGGACCTGAC CCCACGCAAC
  361 GCCCAAAATA ATAACCCCCA CGAACCATAA ACCATTCCCC ATGGGGGACC CCGTCCCTAA
  421 CCCACGGGGC CCGTGGCTAT GGCAGGGCTT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG
  481 CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC
  541 CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA
  601 TGAACAAACG ACCCAACACC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG
  661 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCATC TCCCGGGCAA
  721 ACGTGCGCGC CAGGTCGCAG ATCGTCGGTA TGGAGCCGGG GGTGGTGACG TGGGTCTGGA
  781 CCATCCCGGA GGTAAGTTGC AGCAGGGCGT CCCGGCAGCC GGCGGGCGAT TGGTCGTAAT
  841 CCAGGATAAA GACGTGCATG GGGACGGAGG CGTTTGGCCA AGACGTCCAA GGCCCAGGCA
  901 AACACGTTGT ACAGGTCGCC GTTGGGGGCC AGCAACTCGG GGGGCCCGAA ACAGGGTAAA
  961 TAACGTGTCC CCGATATGGG GTCGTGGGCC CGCGTTGCTC TGGGGCTTCG GCACCCTGGG
 1021 GGCGGGCACG GCCGTTCCCC CGAAAAGCTG TCCCAATCCT CCCGGCCACG GACCCGCCCG
 1081 CCTCCTGCCA GGATACCGGC ACCGTAATGG GCAAGGCCAG CCCGTTAACG CGGCGAAATC
1141 CGCCGCCAGC CAATAGACTA AGGTTCAAAG CTCAGTCCTT CGCTGT
//
Primer: SV40pA3'Element Reverse
Sequence file: RDB15400_A7Ied.seq check
>01692_15400_A7Ie_SV40pA3'ElementReverse_B11_05_ABI24.ab1
    1 GACTCAAGGG GGGGCGGACC TATGGAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG
   61 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT
  121 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC
  181 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG
  241 CGTTGGCCGA TTCATTAATG CAGCTGAACG GTCTGGTTAT AGGTACATTG AGCAACTGAC
  301 TGAAATGCCT CAAAATGTTC TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA
  361 GTGATTTTTT TCTCCATTTT AGCTTCCTTA GCTCCTGAAA ATCTCGCCAA GCTTCTGATG
  421 GAATTAGAAC TTGGCAAAAC AATACTGAGA ATGAAGTGTA TGTGGAACAG AGGCTGCTGA
  481 TCTCGTTCTT CAGGCTATGA AACTGACACA TTTGGAAACC ACAGTACTTA GAACCACAAA
  541 GTGGGAATCA AGAGAAAAAC AATGATCCCA CGAGAGATCT ATAGATCTAT AGATCATGAG
  601 TGGGAGGAAT GAGCTGGCCC TTAATTTGGT TTTGCTTGTT TAAATTATGA TATCCAACTA
  661 TGAAACATTA TCATAAAGCA ATAGTAAAGA GCCTTCAGTA AAGAGCAGGC ATTTATCTAA
  721 TCCCACCCCA CCCCCACCCC CGTAGCTCCA ATCCTTCCAT TCAAAATGTA GGTACTCTGT
  781 TCTCACCCTT CTTAACAAAG TATGACAGGA AAAACTTCCA TTTTAGTGGA CATCTTTATT
  841 GTTTAATAGA TCATCAATTT CTGCAGGTCC TGGCCGGGGT CCCGCGGAAA CTCGGCCGTG
  901 GTGACCAATA CAAAACAAAA GCGCTCCTCG TACCAGCGAA GAAAGGGCAG AGATGCCGTA
  961 GTCAGGTTTA GTTCGTCCGG CGGCGCCAGA AATCCGCGCG GTGGGTTTTT GGGGGGTCGG
 1021 GGGGGTGTTT GGCAGCCACA GACGCCCGGG TGTTCGTGTC GCGCCAGTAC ATGCGGGTCC
 1081 AATGCCCAGG CCCATCCAAA ATCATGGGTC CTGTCCTGCT TCAGGTTCCA GTCGGTGGAC
1141 CTGGACCCCC CACGGCCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Kawasaki M., Inducible liver injury in the transgenic rat by expressing liver-specific suicide gene. Biochem. Biophys. Res. Commun., 311, 920-928 (2003). PMID 14623269. [link to RRC of NBRP]
user_report Mizuguchi H., Enhanced antitumor effect and reduced vector dissemination with fiber-modified adenovirus vectors expressing herpes simplex virus thymidine kinase. Cancer Gene Ther., 9, 236-242 (2002). PMID 11896439. [link to RRC of NBRP]