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pCALNLZ (#RDB01680)

A control plasmid to introduce LacZ gene into mammalian chromosome

Alternative name Adenoviral vector by Cre/loxP system
Clone info. A control plasmid to introduce LacZ gene into mammalian chromosome under OFF-exprssion condition, which can be switched ON by the Cre-expressing adenovirus.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 9,215 bp
Selectable markers Amp^r (E. coli)
Gene/insert name E. coli lacZ (beta-D-galactosidase) Genomic DNA
Depositor|Developer Saito, Izumu |
 
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Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。
MTAに書く付加的使用条件:
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01680 pCALNLZ DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCALNLZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01680).

Reference section:

Kanegae, Y., Takamori, K., Sato, Y., Lee, G., Nakai, M., Saito, I., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332. [link to RRC of NBRP]
Kanegae, Y., Lee, G., Sato, Y., Tanaka, M., Nakai, M., Sakaki, T., Sugano, S., Saito, I., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12808_A4Fg.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCAF1
Sequence file: RDB12808_A4Fga.seq check
>01680_12808_A4Fg_pAxCA_F1_A09_03.ab1
    1 TGCCAAGCGA CCCAGGGGTA CCTTCTACAT GTTATGCCTT CTTCTTTTTC CTACCCCTCC
   61 TGGGCACGTG CTGGTTATTG TGCTGTTTCA TCATTTTGGC AAAGAATTGA TTAATTCGAG
  121 CGAACGCGTA TAACTTCGTA TAGCATACAT TATACGAAGT TATCTCGAGT CGGATTTGAT
  181 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG
  241 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG
  301 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA
  361 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC
  421 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG
  481 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG
  541 CCGAGAAAGT ATCCATCATG GCTGATGCTA TGCGGCGGCT GCATACGCTT GATCCGGCTA
  601 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG
  661 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC
  721 TGTTCGCCAG GCCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC
  781 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCCTT TTCTGGATTC ATCGACTGTG
  841 GCCGGCTGGG TGTAGCGGAC CGCTATTCGG ACATAGCGTT TGGCTACCCC CGTGATATTG
  901 CTGAAGAGCC TTGGCGGCGA AATGGGGCTG GACCCGCTTC CTTCGTGCTT TTACGGGTAT
  961 TCGCCCCTTC CCCGATTCCG CAAGCGCAAT CGCCTTTCTA TCGCCTTCCT GGACGAAGTT
 1021 CTTCTTGAGC GGGACTCTGG GGTTTCGAAA TGACTCGGAC CAGAGCCGCA GCCCAAATCT
 1081 TGGCCTTCCA GAGATTTCTG AATTCCCCCC CCCGTCCTTC CATATAAAAA GGGTTGGCCT
 1141 TTCGGAAATC TCGATTATCC CGGGGAAACG CCGGGCTGGG GAATGGATTC TTTCCGATAG
 1201 CGGTCTGGGG GGGAAATATC TAAGAAGCGG TCCCGGGGGA ACCAGCTTCC TTCCTGAATA
1261 G
//
Primer: pAxCA_R1
Sequence file: RDB12808_A4Fgb.seq check
>01680_12808_A4Fg_pAxCA_R1_B09_06.ab1
    1 TTTTTAGCGG GGCATTTGGC CACACCAGCC ACCACCTTCT GATAGGCACG CCTGCACCTG
   61 AGGAGTGAAT TGATTTCGAT CCCCTCGACG GATCCCCCCT GCCCGGTTAT TATTATTTTT
  121 GACACCAGAC CAACTGGTAA TGGTAGCGAC CGGCGCTCAG CTGGAATTCC GCCGATACTG
  181 ACGGGCTCCA GGAGTCGTCG CCACCAATCC CCATATGGAA ACCGTCGATA TTCAGCCATG
  241 TGCCTTCTTC CGCGTGCAGC AGATGGCGAT GGCTGGTTTC CATCAGTTGC TGTTGACTGT
  301 AGCGGCTGAT GTTGAACTGG AAGTCGCCGC GCCACTGGTG TGGGCCATAA TTCAATTCGC
  361 GCGTCCCGCA GCGCAGACCG TTTTCGCTCG GGAAGACGTA CGGGGTATAC ATGTCTGACA
  421 ATGGCAGATC CCAGCGGTCA AAACAGGCGG CAGTAAGGCG GTCGGGATAG TTTTCTTGCG
  481 GCCCTAATCC GAGCCAGTTT ACCCGCTCTG CTACCTGCGC CAGCTGGCAG TTCAGGCCAA
  541 TCCGCGCCGG ATGCGGTGTA TCGCTCGCCA CTTCAACATC AACGGTAATC GCCATTTGAC
  601 CACTACCATC AATCCGGTAG GTTTTCCGGC TGATAAATAA GGTTTTCCCC TGATGCTGCC
  661 ACGCGTGAGC GGTCGTAATC AGCACCGCAT CAGCAAGTGT ATCTGCCGTG CACTGCAACA
  721 ACGCTGCTTC GGCCTGGTAA TGGCCCGCCG CCTTCCAGCG TTCGACCCAG GCGTTAGGGT
  781 CAATGCGGGT CGCTTCACTT ACGCCAATGT CGTTATCCAG CGGTGCACGG GTGAACTGAT
  841 CGCGCAGCGG CGTCAGCAGT TGTTTTTTAT CGCCAATCCA CATCTGTGAA AGAAAGCCTG
  901 ACTGGCGGTT AAATTGCCAA CGCTTATTAC CCAGCTCGAT GCAAAAATCC ATTTCGCTGG
  961 TGGTCAGATG CGGGATGGCG TGGACGCGGC GGGGAGCGTC ACACTGAGGT TTTCCGCCAG
 1021 ACGCCACTGC TGCCAAGGCG CTGATGTGCC CCGGCCTTCT GACCATGCGG TCGCGTTTCG
 1081 GGTTGCACTT ACGCGTACTG TGAGCCAGAG TTTGCTCGGG CGCTCTCGGC TGCGGTAGTT
1141 TCAGCAGGTC AATTCAACTG TTTTACCTTG TTGGGAAAGC CGA
//
Primer: Amp_R
Sequence file: RDB12808_A4Fgc.seq check
>01680_12808_A4Fg_Amp_R_C09_09.ab1
    1 CCTTTCCCCT TTTTCAAATT ATTGGAAGCA TTTATCAGGG TTATTGGTCT CATGAGCGGA
   61 TACATATTTG AATGTATTCT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG
  121 AAAAGTGCCA CCTGGGTCGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG
  181 GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC
  241 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA
  301 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG
  361 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG
  421 ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT
  481 GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT CGAGGTGAGC CCCACGTTCT
  541 GCTTCACTCT CCCCATCTCC CCCCCCTCCC CACCCCCAAT TTTGTATTTA TTTATTTTTT
  601 AATTATTTTG TGCAGCGATG GGGGCGGGGG GGGGGGGGGG GCGCGCGCCA GGCGGGCCGG
661 GTTGGGTCAA GGGGCGGGCC GGG
//
Primer: M13_-40
Sequence file: RDB12808_A4Fgd.seq check
>01680_12808_A4Fg_M13_(-40)_D09_12.ab1
    1 CCAGGCACTC CCGCGGCCGC TCATGATGCA CGGTCTGCGG GTACCGAGCG ACTCGAGATA
   61 ACTTCGTATA ATGTATGCTA TACGAAGTTA TACGCGTTCG GATTGATCCA GACATGATAA
  121 GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT
  181 GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA
  241 TCGATTCTAG ACTAGTTTAA TTAATTTAAA TCGATGGGGT GGGCGAAGAA CTCCAGCATG
  301 AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC
  361 CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG
  421 TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG
  481 CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT
  541 CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGGTCCG
  601 CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT
  661 TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCGCGCCT
  721 TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT
  781 GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT
  841 GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA
  901 TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC
  961 CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCCACAGC TGCGCAAGGG
 1021 AACGCCGTCG TGGCCAGGCC ACGATAGCCG CGCTGCCTCG TCCTGCAGTT TCATTCAGGG
 1081 CACCGGACAG GTCGGGTCTG ACAAAAGGAC CGGGCGCCCT TGCGCTGACA GTGGAACACG
1141 CGGCCATTCA GAGGCCAGGC CGAAATTGTA CCGTGATGCG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332. [link to RRC of NBRP]
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]
reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837. [link to RRC of NBRP]
user_report Fukamachi, K., A novel reporter rat strain that expresses LacZ upon Cre-mediated recombination. Genesis, 51 (4): 268-274 (2013). PMID 23349063. [link to RRC of NBRP]