A control plasmid to introduce LacZ gene into mammalian chromosome
Alternative name | Adenoviral vector by Cre/loxP system |
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Clone info. | A control plasmid to introduce LacZ gene into mammalian chromosome under OFF-exprssion condition, which can be switched ON by the Cre-expressing adenovirus. |
Vector backbone | pCAGGS (Plasmid) |
Size of vector backbone | 9,215 bp |
Selectable markers | Amp^r (E. coli) |
Gene/insert name | E. coli lacZ (beta-D-galactosidase) Genomic DNA |
Depositor|Developer | Saito, Izumu | |
Additional map | ![]() |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)). Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。 MTAに書く付加的使用条件: CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB01680 | pCALNLZ | DNA solution |
Materials & Methods section:
The pCALNLZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01680). |
Reference section:
Kanegae, Y., Takamori, K., Sato, Y., Lee, G., Nakai, M., Saito, I., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332. [link to RRC of NBRP] |
Kanegae, Y., Lee, G., Sato, Y., Tanaka, M., Nakai, M., Sakaki, T., Sugano, S., Saito, I., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB12808_A4Fg.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pAxCAF1 Sequence file: RDB12808_A4Fga.seq ![]() |
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>01680_12808_A4Fg_pAxCA_F1_A09_03.ab1 1 TGCCAAGCGA CCCAGGGGTA CCTTCTACAT GTTATGCCTT CTTCTTTTTC CTACCCCTCC 61 TGGGCACGTG CTGGTTATTG TGCTGTTTCA TCATTTTGGC AAAGAATTGA TTAATTCGAG 121 CGAACGCGTA TAACTTCGTA TAGCATACAT TATACGAAGT TATCTCGAGT CGGATTTGAT 181 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG 241 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG 301 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA 361 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC 421 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG 481 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG 541 CCGAGAAAGT ATCCATCATG GCTGATGCTA TGCGGCGGCT GCATACGCTT GATCCGGCTA 601 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG 661 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC 721 TGTTCGCCAG GCCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 781 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCCTT TTCTGGATTC ATCGACTGTG 841 GCCGGCTGGG TGTAGCGGAC CGCTATTCGG ACATAGCGTT TGGCTACCCC CGTGATATTG 901 CTGAAGAGCC TTGGCGGCGA AATGGGGCTG GACCCGCTTC CTTCGTGCTT TTACGGGTAT 961 TCGCCCCTTC CCCGATTCCG CAAGCGCAAT CGCCTTTCTA TCGCCTTCCT GGACGAAGTT 1021 CTTCTTGAGC GGGACTCTGG GGTTTCGAAA TGACTCGGAC CAGAGCCGCA GCCCAAATCT 1081 TGGCCTTCCA GAGATTTCTG AATTCCCCCC CCCGTCCTTC CATATAAAAA GGGTTGGCCT 1141 TTCGGAAATC TCGATTATCC CGGGGAAACG CCGGGCTGGG GAATGGATTC TTTCCGATAG 1201 CGGTCTGGGG GGGAAATATC TAAGAAGCGG TCCCGGGGGA ACCAGCTTCC TTCCTGAATA 1261 G // |
Primer: pAxCA_R1 Sequence file: RDB12808_A4Fgb.seq ![]() |
>01680_12808_A4Fg_pAxCA_R1_B09_06.ab1 1 TTTTTAGCGG GGCATTTGGC CACACCAGCC ACCACCTTCT GATAGGCACG CCTGCACCTG 61 AGGAGTGAAT TGATTTCGAT CCCCTCGACG GATCCCCCCT GCCCGGTTAT TATTATTTTT 121 GACACCAGAC CAACTGGTAA TGGTAGCGAC CGGCGCTCAG CTGGAATTCC GCCGATACTG 181 ACGGGCTCCA GGAGTCGTCG CCACCAATCC CCATATGGAA ACCGTCGATA TTCAGCCATG 241 TGCCTTCTTC CGCGTGCAGC AGATGGCGAT GGCTGGTTTC CATCAGTTGC TGTTGACTGT 301 AGCGGCTGAT GTTGAACTGG AAGTCGCCGC GCCACTGGTG TGGGCCATAA TTCAATTCGC 361 GCGTCCCGCA GCGCAGACCG TTTTCGCTCG GGAAGACGTA CGGGGTATAC ATGTCTGACA 421 ATGGCAGATC CCAGCGGTCA AAACAGGCGG CAGTAAGGCG GTCGGGATAG TTTTCTTGCG 481 GCCCTAATCC GAGCCAGTTT ACCCGCTCTG CTACCTGCGC CAGCTGGCAG TTCAGGCCAA 541 TCCGCGCCGG ATGCGGTGTA TCGCTCGCCA CTTCAACATC AACGGTAATC GCCATTTGAC 601 CACTACCATC AATCCGGTAG GTTTTCCGGC TGATAAATAA GGTTTTCCCC TGATGCTGCC 661 ACGCGTGAGC GGTCGTAATC AGCACCGCAT CAGCAAGTGT ATCTGCCGTG CACTGCAACA 721 ACGCTGCTTC GGCCTGGTAA TGGCCCGCCG CCTTCCAGCG TTCGACCCAG GCGTTAGGGT 781 CAATGCGGGT CGCTTCACTT ACGCCAATGT CGTTATCCAG CGGTGCACGG GTGAACTGAT 841 CGCGCAGCGG CGTCAGCAGT TGTTTTTTAT CGCCAATCCA CATCTGTGAA AGAAAGCCTG 901 ACTGGCGGTT AAATTGCCAA CGCTTATTAC CCAGCTCGAT GCAAAAATCC ATTTCGCTGG 961 TGGTCAGATG CGGGATGGCG TGGACGCGGC GGGGAGCGTC ACACTGAGGT TTTCCGCCAG 1021 ACGCCACTGC TGCCAAGGCG CTGATGTGCC CCGGCCTTCT GACCATGCGG TCGCGTTTCG 1081 GGTTGCACTT ACGCGTACTG TGAGCCAGAG TTTGCTCGGG CGCTCTCGGC TGCGGTAGTT 1141 TCAGCAGGTC AATTCAACTG TTTTACCTTG TTGGGAAAGC CGA // |
Primer: Amp_R Sequence file: RDB12808_A4Fgc.seq ![]() |
>01680_12808_A4Fg_Amp_R_C09_09.ab1 1 CCTTTCCCCT TTTTCAAATT ATTGGAAGCA TTTATCAGGG TTATTGGTCT CATGAGCGGA 61 TACATATTTG AATGTATTCT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 121 AAAAGTGCCA CCTGGGTCGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG 181 GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC 241 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA 301 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG 361 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG 421 ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT 481 GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT CGAGGTGAGC CCCACGTTCT 541 GCTTCACTCT CCCCATCTCC CCCCCCTCCC CACCCCCAAT TTTGTATTTA TTTATTTTTT 601 AATTATTTTG TGCAGCGATG GGGGCGGGGG GGGGGGGGGG GCGCGCGCCA GGCGGGCCGG 661 GTTGGGTCAA GGGGCGGGCC GGG // |
Primer: M13_-40 Sequence file: RDB12808_A4Fgd.seq ![]() |
>01680_12808_A4Fg_M13_(-40)_D09_12.ab1 1 CCAGGCACTC CCGCGGCCGC TCATGATGCA CGGTCTGCGG GTACCGAGCG ACTCGAGATA 61 ACTTCGTATA ATGTATGCTA TACGAAGTTA TACGCGTTCG GATTGATCCA GACATGATAA 121 GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT 181 GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA 241 TCGATTCTAG ACTAGTTTAA TTAATTTAAA TCGATGGGGT GGGCGAAGAA CTCCAGCATG 301 AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC 361 CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG 421 TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG 481 CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT 541 CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGGTCCG 601 CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT 661 TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCGCGCCT 721 TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT 781 GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT 841 GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA 901 TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC 961 CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCCACAGC TGCGCAAGGG 1021 AACGCCGTCG TGGCCAGGCC ACGATAGCCG CGCTGCCTCG TCCTGCAGTT TCATTCAGGG 1081 CACCGGACAG GTCGGGTCTG ACAAAAGGAC CGGGCGCCCT TGCGCTGACA GTGGAACACG 1141 CGGCCATTCA GAGGCCAGGC CGAAATTGTA CCGTGATGCG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332. [link to RRC of NBRP] |
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original | Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP] |
reference | Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837. [link to RRC of NBRP] |
user_report | Fukamachi, K., A novel reporter rat strain that expresses LacZ upon Cre-mediated recombination. Genesis, 51 (4): 268-274 (2013). PMID 23349063. [link to RRC of NBRP] |
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