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pCALNLZ

A control plasmid to introduce LacZ gene into mammalian chromosome

Catalog number RDB01680
Resource name pCALNLZ
Alternative name Adenoviral vector by Cre/loxP system
Clone info. A control plasmid to introduce LacZ gene into mammalian chromosome under OFF-exprssion condition, which can be switched ON by the Cre-expressing adenovirus.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 9,215 bp
Selectable markers Amp^r (E. coli)
Gene/insert name E. coli lacZ (beta-D-galactosidase) Genomic DNA
Depositor Saito, Izumu |
 
Other clones in our bank E. coli lacZ (KEGG orthology K01190) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01680 pCALNLZ DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01680.gif from Depositor

Featured content

Featured content Cre-loxP and FLP-FRT System (English text)
Featured content Control Vectors (English text)

References

original Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332.
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12808_A4Fg.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCAF1 RDB12808_A4Fga.seq
>01680_12808_A4Fg_pAxCA_F1_A09_03.ab1
    1 TGCCAAGCGA CCCAGGGGTA CCTTCTACAT GTTATGCCTT CTTCTTTTTC CTACCCCTCC
   61 TGGGCACGTG CTGGTTATTG TGCTGTTTCA TCATTTTGGC AAAGAATTGA TTAATTCGAG
  121 CGAACGCGTA TAACTTCGTA TAGCATACAT TATACGAAGT TATCTCGAGT CGGATTTGAT
  181 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG
  241 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG
  301 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA
  361 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC
  421 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG
  481 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG
  541 CCGAGAAAGT ATCCATCATG GCTGATGCTA TGCGGCGGCT GCATACGCTT GATCCGGCTA
  601 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG
  661 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC
  721 TGTTCGCCAG GCCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC
  781 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCCTT TTCTGGATTC ATCGACTGTG
  841 GCCGGCTGGG TGTAGCGGAC CGCTATTCGG ACATAGCGTT TGGCTACCCC CGTGATATTG
  901 CTGAAGAGCC TTGGCGGCGA AATGGGGCTG GACCCGCTTC CTTCGTGCTT TTACGGGTAT
  961 TCGCCCCTTC CCCGATTCCG CAAGCGCAAT CGCCTTTCTA TCGCCTTCCT GGACGAAGTT
 1021 CTTCTTGAGC GGGACTCTGG GGTTTCGAAA TGACTCGGAC CAGAGCCGCA GCCCAAATCT
 1081 TGGCCTTCCA GAGATTTCTG AATTCCCCCC CCCGTCCTTC CATATAAAAA GGGTTGGCCT
 1141 TTCGGAAATC TCGATTATCC CGGGGAAACG CCGGGCTGGG GAATGGATTC TTTCCGATAG
 1201 CGGTCTGGGG GGGAAATATC TAAGAAGCGG TCCCGGGGGA ACCAGCTTCC TTCCTGAATA
1261 G
//
Primer: pAxCA_R1 RDB12808_A4Fgb.seq
>01680_12808_A4Fg_pAxCA_R1_B09_06.ab1
    1 TTTTTAGCGG GGCATTTGGC CACACCAGCC ACCACCTTCT GATAGGCACG CCTGCACCTG
   61 AGGAGTGAAT TGATTTCGAT CCCCTCGACG GATCCCCCCT GCCCGGTTAT TATTATTTTT
  121 GACACCAGAC CAACTGGTAA TGGTAGCGAC CGGCGCTCAG CTGGAATTCC GCCGATACTG
  181 ACGGGCTCCA GGAGTCGTCG CCACCAATCC CCATATGGAA ACCGTCGATA TTCAGCCATG
  241 TGCCTTCTTC CGCGTGCAGC AGATGGCGAT GGCTGGTTTC CATCAGTTGC TGTTGACTGT
  301 AGCGGCTGAT GTTGAACTGG AAGTCGCCGC GCCACTGGTG TGGGCCATAA TTCAATTCGC
  361 GCGTCCCGCA GCGCAGACCG TTTTCGCTCG GGAAGACGTA CGGGGTATAC ATGTCTGACA
  421 ATGGCAGATC CCAGCGGTCA AAACAGGCGG CAGTAAGGCG GTCGGGATAG TTTTCTTGCG
  481 GCCCTAATCC GAGCCAGTTT ACCCGCTCTG CTACCTGCGC CAGCTGGCAG TTCAGGCCAA
  541 TCCGCGCCGG ATGCGGTGTA TCGCTCGCCA CTTCAACATC AACGGTAATC GCCATTTGAC
  601 CACTACCATC AATCCGGTAG GTTTTCCGGC TGATAAATAA GGTTTTCCCC TGATGCTGCC
  661 ACGCGTGAGC GGTCGTAATC AGCACCGCAT CAGCAAGTGT ATCTGCCGTG CACTGCAACA
  721 ACGCTGCTTC GGCCTGGTAA TGGCCCGCCG CCTTCCAGCG TTCGACCCAG GCGTTAGGGT
  781 CAATGCGGGT CGCTTCACTT ACGCCAATGT CGTTATCCAG CGGTGCACGG GTGAACTGAT
  841 CGCGCAGCGG CGTCAGCAGT TGTTTTTTAT CGCCAATCCA CATCTGTGAA AGAAAGCCTG
  901 ACTGGCGGTT AAATTGCCAA CGCTTATTAC CCAGCTCGAT GCAAAAATCC ATTTCGCTGG
  961 TGGTCAGATG CGGGATGGCG TGGACGCGGC GGGGAGCGTC ACACTGAGGT TTTCCGCCAG
 1021 ACGCCACTGC TGCCAAGGCG CTGATGTGCC CCGGCCTTCT GACCATGCGG TCGCGTTTCG
 1081 GGTTGCACTT ACGCGTACTG TGAGCCAGAG TTTGCTCGGG CGCTCTCGGC TGCGGTAGTT
1141 TCAGCAGGTC AATTCAACTG TTTTACCTTG TTGGGAAAGC CGA
//
Primer: Amp_R RDB12808_A4Fgc.seq
>01680_12808_A4Fg_Amp_R_C09_09.ab1
    1 CCTTTCCCCT TTTTCAAATT ATTGGAAGCA TTTATCAGGG TTATTGGTCT CATGAGCGGA
   61 TACATATTTG AATGTATTCT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG
  121 AAAAGTGCCA CCTGGGTCGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG
  181 GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC
  241 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA
  301 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG
  361 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG
  421 ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT
  481 GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT CGAGGTGAGC CCCACGTTCT
  541 GCTTCACTCT CCCCATCTCC CCCCCCTCCC CACCCCCAAT TTTGTATTTA TTTATTTTTT
  601 AATTATTTTG TGCAGCGATG GGGGCGGGGG GGGGGGGGGG GCGCGCGCCA GGCGGGCCGG
661 GTTGGGTCAA GGGGCGGGCC GGG
//
Primer: M13_-40 RDB12808_A4Fgd.seq
>01680_12808_A4Fg_M13_(-40)_D09_12.ab1
    1 CCAGGCACTC CCGCGGCCGC TCATGATGCA CGGTCTGCGG GTACCGAGCG ACTCGAGATA
   61 ACTTCGTATA ATGTATGCTA TACGAAGTTA TACGCGTTCG GATTGATCCA GACATGATAA
  121 GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT
  181 GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA
  241 TCGATTCTAG ACTAGTTTAA TTAATTTAAA TCGATGGGGT GGGCGAAGAA CTCCAGCATG
  301 AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC
  361 CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG
  421 TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG
  481 CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT
  541 CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGGTCCG
  601 CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT
  661 TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCGCGCCT
  721 TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT
  781 GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT
  841 GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA
  901 TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC
  961 CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCCACAGC TGCGCAAGGG
 1021 AACGCCGTCG TGGCCAGGCC ACGATAGCCG CGCTGCCTCG TCCTGCAGTT TCATTCAGGG
 1081 CACCGGACAG GTCGGGTCTG ACAAAAGGAC CGGGCGCCCT TGCGCTGACA GTGGAACACG
1141 CGGCCATTCA GAGGCCAGGC CGAAATTGTA CCGTGATGCG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332.
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-199 (1991). PMID 1660837.
user report Fukamachi, K., A novel reporter rat strain that expresses LacZ upon Cre-mediated recombination. Genesis, 51 (4): 268-274 (2013). PMID 23349063.

2018.12.11

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