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pCALwL

A cassette plasmid to insert cDNA into downstream CAG promoter

Catalog number RDB01679
Resource name pCALwL
Clone info. A cassette plasmid to insert cDNA into downstream CAG promoter under ON-expression condition, which can be switched OFF (i.e., removed) by the Cre-expressing adenovirus.
Vector backbone pCALwL (Plasmid)
Size of vector backbone 4,931 bp
Selectable markers Amp^r (E. coli)
Gene/insert name P1 phage loxP
Depositor Saito, Izumu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01679 pCALwL DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01679.gif from Depositor

Original reference

original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Cre-loxP and FLP-FRT System (English text)
Featured content Control Vectors (English text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12679_A4Dg.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCAF1 | Sequence: RDB12679_A4Dga.seq
>01679_12679_A4Dg_1_pAxCAF1_A04_01.ab1
    1 TGGGCGGCCC GCACGCCCCC GTTGCGTCTC ATGTTGTGCC TTCTTCTTTT TCCTACAGCT
   61 CCTGGGCAAC GTGCTGGTTA TTGTGCTGTC TTATCATTTT GGCAAAGAAT TGATTAATTC
  121 GAGCGAACGC GTATAACTTC GTATAGCATA CATTATACGA AGTTATCTCG AGTCGGATTT
  181 AAATCCGAAC GCGTATAACT TCGTATAGCA TACATTATAC GAAGTTATCT CGAGTCGCTC
  241 GGTACCCGGG GATCAAATCA ATTCACTCCT CAGGTGCAGG CTGCCTATCA GAAGGTGGTG
  301 GCTGGTGTGG CCAATGCCCT GGCTCACAAA TACCACTGAG ATCTTTTTCC CTCTGCCAAA
  361 AATTATGGGG ACATCATGAA GCCCCTTGAG CATCTGACTT CTGGCTAATA AAGGAAATTT
  421 ATTTTCATTG CAATAGTGTG TTGGAATTTT TTGTGTCTCT CACTCGGAAG GACATATGGG
  481 AGGGCAAATC ATTTAAAACA TCAGAATGAG TATTTGGTTT AGAGTTTGGC AACATATGCC
  541 CATATGCTGG CTGCCATGAA CAAAGGTTGG CTATAAAGAG GTCATCAGTA TATGAAACAG
  601 CCCCCTGCTG TCCATTCCTT ATTCCATAGA AAAGCCTTGA CTTGAGGTTA GATTTTTTTT
  661 ATATTTTGTT TTGTGTTATT TTTTTCTTTA ACATCCCTAA AATTTTCCTT ACATGTTTTA
  721 CTAGCCAGAT TTTTCCTCCT CTCCTGACTA CTCCCAGTCA TAGCTGTCCC TCTTCTCTTA
  781 TGGAGATCCC TCGACCTGCA GCCCAAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT
  841 GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCCGGGAAG CATAAAGTGT
  901 AAAGCCTGGG GTGCCCTAAT GAGTGAGCTA AACTCACATT TAATTGCGTT GCGCTCACTG
  961 CCCGCTTTCA AGTCGGGGAA ACCCTGTCGT GCCAGCGGGA TCCGGCATCT CAATTTAGTC
 1021 AGCCAAACCA TAGGTCCCGC CCCCTAAACT TCCGCCCAAG TTCCCGCCCC AATTCTCCGG
 1081 CCCCATGGGC TGGACCTAAA TTTTTTTTTA ATTTATGCAG GAGGCCCGGA GCGGCCTCCG
1141 GCTCTGAGCT ATCCAGAAGG TAGTGAAGGA AGGCCTTCTT TTTGTGGAAG GG
//
Primer: pAxCA_R1 | Sequence: RDB12679_A4Dgb.seq
>01679_12679_A4Dg_1_pAxCAR1_B04_04.ab1
    1 TAACTGAAGG GGCAATTGGG CCCACCAGGC CACCACCTTT CTGATAGGCA GCCTGCACCT
   61 GAGGAGTGAA TTGATTTGAT CCCCGGGTAC CGAGCGACTC GAGATAACTT CGTATAATGT
  121 ATGCTATACG AAGTTATACG CGTTCGGATT TAAATCCGAC TCGAGATAAC TTCGTATAAT
  181 GTATGCTATA CGAAGTTATA CGCGTTCGCT CGAATTAATC AATTCTTTGC CAAAATGATG
  241 AGACAGCACA ATAACCAGCA CGTTGCCCAG GAGCTGTAGG AAAAAGAAGA AGGCATGAAC
  301 ATGGTTAGCA GAGGCTCTAG AGCCGCCGGT CACACGCCAG AAGCCGAACC CCGCCCTGCC
  361 CCGTCCCCCC CGAAGGCAGC CGTCCCCCCG CGGACAGCCC CGAGGCTGGA GAGGGAGAAG
  421 GGGACGGCGG CGCGGCGACG CACGAAGGCC CTCCCCGCCC ATTTCCTTCC TGCCGGCGCC
  481 GCACCGCTTC GCCCCGCGCC CGCTAGAGGG GGTGCGGCGG CGCCTCCCAG ATTTCGGCTC
  541 CGCACAGATT TGGGACAAAG GAAGTCCCTG CGCCCTCTCG CACGATTACC ATAAAAGGCA
  601 ATGGCTGCGG CTCGCCGCGC CTCGACAGCC GCCGGCGCTC CGGGGGCCGC CGCGCCCCTC
  661 CCCCGAGCCC TCCCCGGCCC GAGGCGGCCC CGCCCCGCCC GGCACCCCCA CCTGCCGCCA
  721 CCCCCCGCCC GGCCGGCGAG CCCCTCGCCA CGCCCCGTAC GGGAGCCCCG CACCCGAAAG
781 CCGGGCCGTG CTCAGCACCT CGGGGAGGGG GGGTGCAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-199 (1991). PMID 1660837.
user report Mitsui, S., A mental retardation gene, motopsin/prss12, modulates cell morphology by interaction with seizure-related gene 6. Biochem. Biophys. Res. Commun., 436 (4): 638-644 (2013). PMID 23770366.
user report Maeda M., Targeted gene therapy toward astrocytoma using a Cre/loxP-based adenovirus system. Brain Res., 1081, 34-43 (2006). PMID 16529724.
user report Kamei, K., Transgenic mouse for conditional, tissue-specific Cox-2 overexpression Genesis, 44, 177-182 (2006). PMID 16604526.
user report Kudo T., Transgenic expression of a dominant-negative connexin26 causes degeneration of the organ of Corti and non-syndromic deafness. Hum. Mol. Genet., 12, 995-1004 (2003). PMID 12700168.
user report Honma M., Developmental alteration of nerve injury induced glial cell line-derived neurotrophic factor (GDNF) receptor expression is crucial for the determination of injured motoneuron fate. J. Neurochem., 82, 961-975 (2002). PMID 12358802.
user report Nagayama Y., Targeting the replication of adenovirus to p53-defective thyroid carcinoma with a p53-regulated Cre/loxP system. Cancer Gene Ther., 8, 36-44 (2001). PMID 11219492.

2019.11.02

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