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pCALwL

A cassette plasmid to insert cDNA into downstream CAG promoter

Catalog number RDB01679
Resource name pCALwL
Clone info. A cassette plasmid to insert cDNA into downstream CAG promoter under ON-expression condition, which can be switched OFF (i.e., removed) by the Cre-expressing adenovirus.
Vector backbone pCALwL (Plasmid)
Size of vector backbone 4,931 bp
Selectable markers Amp^r (E. coli)
Gene/insert name P1 phage loxP
Depositor Saito, Izumu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01679 pCALwL DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01679.gif from Depositor

Featured content

Featured content Cre-loxP and FLP-FRT System (English text)
Featured content Control Vectors (English text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

References

original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

User reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16804_A8Lpp2-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAGGS_R1 RDB16804_A8Lpa.seq
>01679_16804_A8Lp_3_pCAGGS_R1_G02_20_ABI24.ab1
    1 CGGCAAAAAA TTCCACACAC TATTGCAATG AAAATAAATT TCCTTTATTA GCCAGAAGTC
   61 AGATGCTCAA GGGGCTTCAT GATGTCCCCA TAATTTTTGG CAGAGGGAAA AAGATCTCAG
  121 TGGTATTTGT GAGCCAGGGC ATTGGCCACA CCAGCCACCA CCTTCTGATA GGCAGCCTGC
  181 ACCTGAGGAG TGAATTGATT TGATCCCCGG GTACCGAGCG ACTCGAGATA ACTTCGTATA
  241 ATGTATGCTA TACGAAGTTA TACGCGTTCG GATTTAAATC CGACTCGAGA TAACTTCGTA
  301 TAATGTATGC TATACGAAGT TATACGCGTT CGCTCGAATT AATCAATTCT TTGCCAAAAT
  361 GATGAGACAG CACAATAACC AGCACGTTGC CCAGGAGCTG TAGGAAAAAG AAGAAGGCAT
  421 GAACATGGTT AGCAGAGGCT CTAGAGCCGC CGGTCACACG CCAGAAGCCG AACCCCGCCC
  481 TGCCCCGTCC CCCCCGAAGG CAGCCGTCCC CCCGCGGACA GCCCCGAGGC TGGAGAGGGA
  541 GAAGGGGACG GCGGCGCGGC GACGCACGAA GGCCCTCCCC GCCCATTTCC TTCCTGCCGG
  601 CGCCGCACCG CTTCGCCCCG CGCCCGCTAG AGGGGGTGCG GCGGCGCCTC CCAGATTTCG
  661 GCTCCGCACA GATTTGGGAC AAAGGAAGTC CCTGCGCCCT CTCGCACGAT TACCATAAAA
  721 GGCAATGGCT GCGGCTCGCC GCGCCTCGAC AGCCGCCGGC GCTCCGGGGG CCGCCGCGCC
781 CCTCCCCCGA GCC
//

Please visit Sequencing and PCR primers for primer information.

References

References

original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-199 (1991). PMID 1660837.
user report Mitsui, S., A mental retardation gene, motopsin/prss12, modulates cell morphology by interaction with seizure-related gene 6. Biochem. Biophys. Res. Commun., 436 (4): 638-644 (2013). PMID 23770366.
user report Maeda M., Targeted gene therapy toward astrocytoma using a Cre/loxP-based adenovirus system. Brain Res., 1081, 34-43 (2006). PMID 16529724.
user report Kamei, K., Transgenic mouse for conditional, tissue-specific Cox-2 overexpression Genesis, 44, 177-182 (2006). PMID 16604526.
user report Kudo T., Transgenic expression of a dominant-negative connexin26 causes degeneration of the organ of Corti and non-syndromic deafness. Hum. Mol. Genet., 12, 995-1004 (2003). PMID 12700168.
user report Honma M., Developmental alteration of nerve injury induced glial cell line-derived neurotrophic factor (GDNF) receptor expression is crucial for the determination of injured motoneuron fate. J. Neurochem., 82, 961-975 (2002). PMID 12358802.
user report Nagayama Y., Targeting the replication of adenovirus to p53-defective thyroid carcinoma with a p53-regulated Cre/loxP system. Cancer Gene Ther., 8, 36-44 (2001). PMID 11219492.

2019.01.17

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