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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCALwL

A cassette plasmid to insert cDNA into downstream CAG promoter

Catalog number RDB01679
Resource name pCALwL
Clone info. A cassette plasmid to insert cDNA into downstream CAG promoter under ON-expression condition, which can be switched OFF (i.e., removed) by the Cre-expressing adenovirus.
Vector backbone pCALwL (Plasmid)
Size of vector backbone 4,931 bp
Selectable markers Amp^r (E. coli)
Gene/insert name P1 phage loxP
Depositor|Developer Saito, Izumu |
Other clones in our bank

External Database
P1 phage loxP

            Reference sequence
              
             
            Remarks, protocol and/or map (gif) RDB01679.gif

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
            Additional terms and conditions:
            Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。
            付加的提供条件:
            CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB01679 pCALwL DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)
            Featured content Expression regulation (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB16804_A8Lpp2-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pCAGGS_R1
            Sequence file: RDB16804_A8Lpa.seq check
            >01679_16804_A8Lp_3_pCAGGS_R1_G02_20_ABI24.ab1
                1 CGGCAAAAAA TTCCACACAC TATTGCAATG AAAATAAATT TCCTTTATTA GCCAGAAGTC
               61 AGATGCTCAA GGGGCTTCAT GATGTCCCCA TAATTTTTGG CAGAGGGAAA AAGATCTCAG
              121 TGGTATTTGT GAGCCAGGGC ATTGGCCACA CCAGCCACCA CCTTCTGATA GGCAGCCTGC
              181 ACCTGAGGAG TGAATTGATT TGATCCCCGG GTACCGAGCG ACTCGAGATA ACTTCGTATA
              241 ATGTATGCTA TACGAAGTTA TACGCGTTCG GATTTAAATC CGACTCGAGA TAACTTCGTA
              301 TAATGTATGC TATACGAAGT TATACGCGTT CGCTCGAATT AATCAATTCT TTGCCAAAAT
              361 GATGAGACAG CACAATAACC AGCACGTTGC CCAGGAGCTG TAGGAAAAAG AAGAAGGCAT
              421 GAACATGGTT AGCAGAGGCT CTAGAGCCGC CGGTCACACG CCAGAAGCCG AACCCCGCCC
              481 TGCCCCGTCC CCCCCGAAGG CAGCCGTCCC CCCGCGGACA GCCCCGAGGC TGGAGAGGGA
              541 GAAGGGGACG GCGGCGCGGC GACGCACGAA GGCCCTCCCC GCCCATTTCC TTCCTGCCGG
              601 CGCCGCACCG CTTCGCCCCG CGCCCGCTAG AGGGGGTGCG GCGGCGCCTC CCAGATTTCG
              661 GCTCCGCACA GATTTGGGAC AAAGGAAGTC CCTGCGCCCT CTCGCACGAT TACCATAAAA
              721 GGCAATGGCT GCGGCTCGCC GCGCCTCGAC AGCCGCCGGC GCTCCGGGGG CCGCCGCGCC
            781 CCTCCCCCGA GCC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
            reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837.
            user_report Mitsui, S., A mental retardation gene, motopsin/prss12, modulates cell morphology by interaction with seizure-related gene 6. Biochem. Biophys. Res. Commun., 436 (4): 638-644 (2013). PMID 23770366.
            user_report Maeda M., Targeted gene therapy toward astrocytoma using a Cre/loxP-based adenovirus system. Brain Res., 1081, 34-43 (2006). PMID 16529724.
            user_report Kamei, K., Transgenic mouse for conditional, tissue-specific Cox-2 overexpression Genesis, 44, 177-182 (2006). PMID 16604526.
            user_report Kudo T., Transgenic expression of a dominant-negative connexin26 causes degeneration of the organ of Corti and non-syndromic deafness. Hum. Mol. Genet., 12, 995-1004 (2003). PMID 12700168.
            user_report Honma M., Developmental alteration of nerve injury induced glial cell line-derived neurotrophic factor (GDNF) receptor expression is crucial for the determination of injured motoneuron fate. J. Neurochem., 82, 961-975 (2002). PMID 12358802.
            user_report Nagayama Y., Targeting the replication of adenovirus to p53-defective thyroid carcinoma with a p53-regulated Cre/loxP system. Cancer Gene Ther., 8, 36-44 (2001). PMID 11219492.

            2022.10.11

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