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pAxCAwt (#RDB01678)

A cassette cosmid for construction of a recombinant adenovirus

Alternative name pAdex1CAwt
Clone info. A cassette cosmid for construction of a recombinant adenovirus expressing cDNA under the control of CAG promoter.
Comment The pAxCAwt cosmid [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996] is a derivative of pAxcw (RDB 917) and includes a cassette that consists of the expression unit under the control of a modified promoter of the chicken gene for beta-actin with a cytomegalovirus immediate-early enhancer (CMV-IE), designated the CA promoter [Niwa et al., Gene 108, 193-99, 1991], and a polyadenylation sequence from the rabbit gene for beta-globin (G polyA), namely a CAG cassette, with a unique SwaI site, cloned at the E1 deletion. The pAxCAwt cosmid can be used to generate recombinant adenovirus by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996].
Vector backbone pAxcw (Cosmid, use packaging extracts for transforming E. coli host)
Size of vector backbone 44,820 bp
Selectable markers Amp^r
Gene/insert name AdV_5 - Genomic DNA
Depositor|Developer Saito, Izumu |
 
Additional map
Remarks, protocol and/or map (pdf) RDB01678.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB01678 pAxCAwt Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pAxCAwt was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01678).

Reference section:

Miyake, S., Makimura, M., Kanegae, Y., Harada, S., Sato, Y., Takamori, K., Tokuda, C., Saito, I., Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. USA, 93, 1320-1324 (1996). PMID 8577762. [link to RRC of NBRP]
Kanegae, Y., Lee, G., Sato, Y., Tanaka, M., Nakai, M., Sakaki, T., Sugano, S., Saito, I., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Miyake, S., Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. USA, 93, 1320-1324 (1996). PMID 8577762. [link to RRC of NBRP]
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]
reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837. [link to RRC of NBRP]
user_report Kida, J., Interaction of LEF1 with TAZ is necessary for the osteoblastogenic activity of Wnt3a. Sci. Rep. 8 (1): 10375 (2018). PMID 29991769. [link to RRC of NBRP]
user_report Zhu P., Ramified microglial cells promote astrogliogenesis and maintenance of neural stem cells through activation of Stat3 function. FASEB J., 22, 3866-3877 (2008). PMID 18685078. [link to RRC of NBRP]
user_report Cao, F., Overexpression of SOCS3 inhibits astrogliogenesis and promotes maintenance of neural stem cells J. Neurochem., 98, 459–470 (2006). PMID 16805839. [link to RRC of NBRP]
user_report Gu F., Suppression of Stat3 promotes neurogenesis in cultured neural stem cells. J. Neurosci. Res., 81, 163-171 (2005). PMID 15948155. [link to RRC of NBRP]
user_report Nakano, N., Downregulation of cyclin-dependent kinase inhibitor; p57(kip2), is involved in the cell cycle progression of vascular smooth muscle cell. Biochem. Biophys. Res. Commun., 338, 1661-1667 (2005). PMID 16259944. [link to RRC of NBRP]
user_report Ugai H., Spontaneous mutations in the human gene for p53 in recombinant adenovirus during multiple passages in human embryonic kidney 293 cells. Biochem. Biophys. Res. Commun., 300, 448-456. (2003). PMID 12504105. [link to RRC of NBRP]
user_report Baba, M., Tumor suppressor protein VHL is induced at high cell density and mediates contact inhibition of cell growth. Oncogene, 20 (22): 2727-2736 (2001). PMID 11420685. [link to RRC of NBRP]
user_report Takatsu H., Overexpression of EC-SOD suppresses endothelial-cell-mediated LDL oxidation. Biochem. Biophys. Res. Commun., 285, 84-91 (2001). PMID 11437376. [link to RRC of NBRP]
user_report Yamanaka T., PAR-6 regulates aPKC activity in a novel way and mediates cell-cell contact-induced formation of the epithelial junctional complex. Genes Cells, 6, 721-731 (2001). PMID 11532031. [link to RRC of NBRP]