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pAxCAwt

A cassette cosmid for construction of a recombinant adenovirus

Catalog number RDB01678
Resource name pAxCAwt
Alternative name pAdex1CAwt
Clone info. A cassette cosmid for construction of a recombinant adenovirus expressing cDNA under the control of CAG promoter.
The pAxCAwt cosmid [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996] is a derivative of pAxcw (RDB 917) and includes a cassette that consists of the expression unit under the control of a modified promoter of the chicken gene for beta-actin with a cytomegalovirus immediate-early enhancer (CMV-IE), designated the CA promoter [Niwa et al., Gene 108, 193-99, 1991], and a polyadenylation sequence from the rabbit gene for beta-globin (G polyA), namely a CAG cassette, with a unique SwaI site, cloned at the E1 deletion. The pAxCAwt cosmid can be used to generate recombinant adenovirus by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996].
Vector backbone pAxcw (Cosmid, use packaging extracts for transformation)
Size of vector backbone 44,820 bp
Selectable markers Amp^r
Gene/insert name AdV_5 - Genomic DNA
Depositor Saito, Izumu |

Distribution information

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Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
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Remarks ((Additional form)) CAG(FormE)
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RDB01678 pAxCAwt DNA solution

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References

original Miyake, S., Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. U S A., 93, 1320-1324 (1996). PMID 8577762.
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

User reports and related articles (go to bottom)


Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

References

original Miyake, S., Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. U S A., 93, 1320-1324 (1996). PMID 8577762.
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-199 (1991). PMID 1660837.
user report Kida, J., Interaction of LEF1 with TAZ is necessary for the osteoblastogenic activity of Wnt3a. Sci. Rep. 8 (1): 10375 (2018). PMID 29991769.
user report Zhu P., Ramified microglial cells promote astrogliogenesis and maintenance of neural stem cells through activation of Stat3 function. FASEB J., 22, 3866-3877 (2008). PMID 18685078.
user report Cao, F., Overexpression of SOCS3 inhibits astrogliogenesis and promotes maintenance of neural stem cells J. Neurochem., 98, 459–470 (2006). PMID 16805839.
user report Gu F., Suppression of Stat3 promotes neurogenesis in cultured neural stem cells. J. Neurosci. Res., 81, 163-171 (2005). PMID 15948155.
user report Nakano, N., Downregulation of cyclin-dependent kinase inhibitor; p57(kip2), is involved in the cell cycle progression of vascular smooth muscle cell. Biochem. Biophys. Res. Commun., 338, 1661-1667 (2005). PMID 16259944.
user report Ugai H., Spontaneous mutations in the human gene for p53 in recombinant adenovirus during multiple passages in human embryonic kidney 293 cells. Biochem. Biophys. Res. Commun., 300, 448-456. (2003). PMID 12504105.
user report Baba, M., Tumor suppressor protein VHL is induced at high cell density and mediates contact inhibition of cell growth. Oncogene, 20 (22): 2727-2736 (2001). PMID 11420685.
user report Takatsu H., Overexpression of EC-SOD suppresses endothelial-cell-mediated LDL oxidation. Biochem. Biophys. Res. Commun., 285, 84-91 (2001). PMID 11437376.
user report Yamanaka T., PAR-6 regulates aPKC activity in a novel way and mediates cell-cell contact-induced formation of the epithelial junctional complex. Genes Cells, 6, 721-731 (2001). PMID 11532031.

2019.03.08

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