Resource data sheet
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pULwL

A cassette plasmid to insert stuffer DNA between two loxP site

Catalog number RDB01677
Resource name pULwL
Clone info. A cassette plasmid to insert stuffer DNA between two loxP site of the same orientation.
Vector backbone pUC119 (Plasmid)
Size of vector backbone 3,276 bp
Selectable markers Amp^r
Gene/insert name P1 phage loxP -
Depositor Saito, Izumu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01677 pULwL DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01677.gif from Depositor

Featured content

Featured content Cre-loxP and FLP-FRT System (English text)
Featured content Control Vectors (English text)

References

original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

User reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB01677_A4Dg.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB01677_A4Dga.seq
>01677_01677_A4Dg_3_M13(-40)_C03_09.ab1
    1 CCCGGCCAAT GATTCGAGCG GCTCGAGATA ACTTCGTATA ATGTATGCTA TACGAAGTTA
   61 TACGCGTTCG GATTTAAATC CGACTCGAGA TAACTTCGTA TAATGTATGC TATACGAAGT
  121 TATACGCGTT CGCTCGGTAC CCGGGGATCC TCTAGAGTCG ACCTGCAGGC ATGCAAGCTT
  181 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA
  241 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT
  301 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT
  361 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC
  421 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA
  481 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG
  541 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA
  601 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
  661 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
  721 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
  781 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
  841 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACGCTGCG CTATCTGGTA CTAATCGTCT
  901 GAGTCAACCC GGTAGACACG ACTTATCGTC ACTGCAGCAG CACTCGTTGA CAGAATAGTC
  961 AAGCGAGGTA TGTAGCCGTG CTACCAAGTC TGCATAGTGG GCGTAACTAC CGCTACATAC
 1021 AGACCGCATG TTGTCTGGCT TGCTGAGCAC GTACTTCCTG AAAGAATGTA GCTCTGATGC
1081 GCCAACACCT GTGACCGTTG TTGCTAGCAC GAA
//

Please visit Sequencing and PCR primers for primer information.

References

References

original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
user report Komatsu, M., Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism. Proc. Natl. Acad. Sci. U S A., 107 (6), 2646-2651 (2010). PMID 20133795.

2018.12.10

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