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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pULwL (#RDB01677)

A cassette plasmid to insert stuffer DNA between two loxP site

Clone info. A cassette plasmid to insert stuffer DNA between two loxP site of the same orientation.
Vector backbone pUC119 (Plasmid)
Size of vector backbone 3,276 bp
Selectable markers Amp^r
Gene/insert name P1 phage loxP -
Depositor|Developer Saito, Izumu |
 
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Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01677 pULwL DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pULwL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01677).

Reference section:

Kanegae, Y., Lee, G., Sato, Y., Tanaka, M., Nakai, M., Sakaki, T., Sugano, S., Saito, I., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB01677_A4Dg.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB01677_A4Dga.seq check
>01677_01677_A4Dg_3_M13(-40)_C03_09.ab1
    1 CCCGGCCAAT GATTCGAGCG GCTCGAGATA ACTTCGTATA ATGTATGCTA TACGAAGTTA
   61 TACGCGTTCG GATTTAAATC CGACTCGAGA TAACTTCGTA TAATGTATGC TATACGAAGT
  121 TATACGCGTT CGCTCGGTAC CCGGGGATCC TCTAGAGTCG ACCTGCAGGC ATGCAAGCTT
  181 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA
  241 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT
  301 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT
  361 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC
  421 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA
  481 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG
  541 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA
  601 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
  661 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
  721 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
  781 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
  841 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACGCTGCG CTATCTGGTA CTAATCGTCT
  901 GAGTCAACCC GGTAGACACG ACTTATCGTC ACTGCAGCAG CACTCGTTGA CAGAATAGTC
  961 AAGCGAGGTA TGTAGCCGTG CTACCAAGTC TGCATAGTGG GCGTAACTAC CGCTACATAC
 1021 AGACCGCATG TTGTCTGGCT TGCTGAGCAC GTACTTCCTG AAAGAATGTA GCTCTGATGC
1081 GCCAACACCT GTGACCGTTG TTGCTAGCAC GAA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]
user_report Komatsu, M., Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism. Proc. Natl. Acad. Sci. U S A., 107 (6), 2646-2651 (2010). PMID 20133795. [link to RRC of NBRP]