RDB01675 - pxCANCre

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pxCANCre

A plasmid expressing the recombinase Cre tagged with a nls


Catalog number	RDB01675
Resource name	pxCANCre
Clone info.	A plasmid expressing the recombinase Cre tagged with a nuclear localization signal under the control of CAG promoter
Vector backbone	pxCAwt (deleted from pAxCAwt with Sal I) (Plasmid)
Size of vector backbone	6,565 bp
Selectable markers	Amp^r
Gene/insert name	P1 phage Cre recombinase cDNA
Depositor\|Developer	Saito, Izumu \|
Other clones in our bank	P1 phage cre \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).

Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

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Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01675	pxCANCre	DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original	Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332.
original	Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

Further references such as user reports and related articles (go to bottom)

Featured content	Cre-loxP and FLP-FRT System (English text)
Featured content	Dnaconda's recommendation EXR0045e (English text)
Featured content	Dnaconda's recommendation EXR0045j (Japanese text)
Featured content	Control Vectors (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14151_A6Ebp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Region: SV40 NLS, insert 5' Sequence file: RDB14151_A6Eba.seq
>01675_14151_A6Eb_3_pAxCA_F1_C08_08.ab1 1 TAAGCTATCA TCTGCTACAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG 61 TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT GATTTATCGA TTTGCAGACC 121 GTGCATCATG AGCGGCCCTC CAAAAAAGAA GAGAAAGGTA GAAGACCCGG GCGGCCGCAT 181 GTCCAATTTA CTGACCGTAC ACCAAAATTT GCCTGCATTA CCGGTCGATG CAACGAGTGA 241 TGAGGTTCGC AAGAACCTGA TGGACATGTT CAGGGATCGC CAGGCGTTTT CTGAGCATAC 301 CTGGAAAATG CTTCTGTCCG TTTGCCGGTC GTGGGCGGCA TGGTGCAAGT TGAATAACCG 361 GAAATGGTTT CCCGCAGAAC CTGAAGATGT TCGCGATTAT CTTCTATATC TTCAGGCGCG 421 CGGTCTGGCA GTAAAAACTA TCCAGCAACA TTTGGGCCAG CTAAACATGC TTCATCGTCG 481 GTCCGGGCTG CCACGACCAA GTGACAGCAA TGCTGTTTCA CTGGTTATGC GGCGGATCCG 541 AAAAGAAAAC GTTGATGCCG GTGAACGTGC AAAACAGGCT CTAGCGTTCG AACGCACTGA 601 TTTCGACCAG GTTCGTTCAC TCATGGAAAA TAGCGATCGC TGCCAGGATA TACGTAATCT 661 GGCATTTCTG GGGATTGCTT ATAACACCCT GTTACGTATA GCCGAAATTG CCAGGATCAG 721 GGTTAAAGAT ATCTCACGTA CTGACGGTGG GAGAATGTTA ATCCATATTG GCAGAACGAA 781 CACGCTGGTT AGCACCGCAG GTGTAGAGAA GGCACTTAGC CTGGGGGTAA CTAAACTGGT 841 CGAGCGATGG ATTTCCGTCT CTGGTGTAGC TGATGATCCG AATAACTACC TGTTTTGCCG 901 GGTCAGAAAA AAATGGTGTT GCCGCGCCAT CTGCCCACCA GCCAGCTATC AAACTCGCGC 961 CCTGGAAAGG ATTTTTTTGA AGCAACCTCA TCGATTGAAT TTTACGGCGC TACGATGGAC 1021 TCTGGGTTCA GAGGATACCT TGGTCCTGAT CTGACACAGT GGTCCGATGA TCCGGAGCCG 1081 GCGCCGA //
Primer: pAxCA_R1 Region: insert 3' Sequence file: RDB14151_A6Ebb.seq
>01675_14151_A6Eb_3_pAxCA_R1_F08_17.ab1 1 GGACATGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC CTGAGGAGTG 61 AATTGATTAT CGATTCTAGA CTAGTTTAAT TAATTTCTAG AGCGCTTAAT GGCTAATCGC 121 CATCTTCCAG CAGGCGCACC ATTGCCCCTG TTTCACTATC CAGGTTACGG ATATAGTTCA 181 TGACAATATT TACATTGGTC CAGCCACCAG CTTGCATGAT CTCCGGTATT GAAACTCCAG 241 CGCGGGCCAT ATCTCGCGCG GCTCCGACAC GGGCACTGTG TCCAGACCAG GCCAGGTATC 301 TCTGACCAGA GTCATCCTTA GCGCCGTAAA TCAATCGATG AGTTGCTTCA AAAATCCCTT 361 CCAGGGCGCG AGTTGATAGC TGGCTGGTGG CAGATGGCGC GGCAACACCA TTTTTTCTGA 421 CCCGGCAAAA CAGGTAGTTA TTCGGATCAT CAGCTACACC AGAGACGGAA ATCCATCGCT 481 CGACCAGTTT AGTTACCCCC AGGCTAAGTG CCTTCTCTAC ACCTGCGGTG CTAACCAGCG 541 TTTTCGTTCT GCCAATATGG ATTAACATTC TCCCACCGTC AGTACGTGAG ATATCTTTAA 601 CCCTGATCCT GGCAATTTCG GCTATACGTA ACAGGGTGTT ATAAGCAATC CCCAGAAATG 661 CCAGATTACG TATATCCTGG CAGCGATCGC TATTTTCCAT GAGTGAACGA ACCTGGTCGA 721 AATCAGTGCG TTCGAACGCT AGAGCCTGTT TTGCACGTTC ACCGGCATCA ACGTTTTTCT 781 TTTCGGATCC GCCGCATAAC CAGTGAAACA GCATTGCTGT CACTTGGTCG TGGCAGCCCG 841 GACCGACGAT GAAGCATGTT TAGCTGGCCC AAATGTTGCT GGATAGTTTT TACTGCCAGA 901 CCGCGCGCCT GAAGATATAG AAGATAATCG CGAACATCTT CAGGTTTCTG CGGGAAACCC 961 ATTTTCCGGT TATTCACTTT GCACCATGAC GCCCACGAAC GGCCAACCGG ACAGAAGGCA 1021 TTTTGCAGCT ATGCTCAGAA ACGGCTTACG ATGCCATGCA AGCATGATCC AATTC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332.
original	Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
reference	Matsuda, T., Controlled expression of transgenes introduced by in vivo electroporation. Proc. Natl. Acad. Sci. U.S.A. 104 (3): 1027-1032 (2007). PMID 17209010.
reference	Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-199 (1991). PMID 1660837.
user report	Nakahara, T., Activation of NF-κB by Human Papillomavirus 16 E1 Limits E1-Dependent Viral Replication through Degradation of E1. J. Virol. 89 (9): 5040-5059 (2015). PMID 25717108.
user report	Sakai, Y., A novel transgenic mouse model carrying human Tribbles related protein 3 (TRB3) gene and its site specific phenotype. Biol. Pharm. Bull., 37 (6): 1068-1074 (2014). PMID 24882419.
user report	Nodagashira T., Efficient Gene Transduction in HER2-Expressing Cells by Two Recombinant Adenovirus Vectors with HER2 Promotor and Cre/loxP System. Hirosaki Med. J., 61: 26-34 (2010).
user report	Kameyama Y., An accumulative site-specific gene integration system using Cre recombinase-mediated cassette exchange. Biotechnol. Bioeng., 105, 1106-1114 (2010). PMID 19998287.
user report	Marchetti G., Integrin alpha5beta1 is necessary for regulation of radial migration of cortical neurons during mouse brain development. Eur. J. Neurosci., 31, 399-409, (2010). PMID 20105241.
user report	Komatsu, M., Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism. Proc. Natl. Acad. Sci. U S A., 107 (6), 2646-2651 (2010). PMID 20133795.
user report	Shimada H., Introduction of tau mutation into cultured Rat1-R12 cells by gene targeting, using recombinant adeno-associated virus vector. Cell. Mol. Neurobiol., 29, 699-705 (2009). PMID 19305950.
user report	Long N., Construction of a multi-functional helper-dependent adenovirus based system for cancer gene therapy. Asian Pac. J. Cancer Prev., 10, 939-960 (2009). PMID 20104994.
user report	Pihkala P., An antigen-mediated selection system for mammalian cells that produce glycosylated single-chain Fv. Biochem. Biophys. Res. Commun., 324, 1165-1172 (2004). PMID 15504336.
user report	Kurihara H., Glioma/glioblastoma-specific adenoviral gene expression using the nestin gene regulator. Gene Ther., 7, 686-693 (2000). PMID 10800092.

2020.10.02

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