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pxCANCre (#RDB01675)

A plasmid expressing the recombinase Cre tagged with a nls


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Kanegae, Y., Gene, 181, 207-212 (1996). PMID 8973332. [PubMed] [Article] [RRC of NBRP]
Publication Kanegae, Y., Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. A plasmid expressing the recombinase Cre tagged with a nuclear localization signal under the control of CAG promoter
Vector backbone pxCAwt (deleted from pAxCAwt with Sal I) (Plasmid)
Size of vector backbone 6,565 bp
Selectable markers Amp^r
Growth conditions 37 C, LB+Amp
Gene/insert name P1 phage Cre recombinase cDNA
Depositor|Developer Saito, Izumu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。
付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee
RDB01675 pxCANCre DNA solution

How to cite this biological resource

Materials & Methods section:

The pxCANCre was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01675).

Reference section:

Kanegae, Y., Takamori, K., Sato, Y., Lee, G., Nakai, M., Saito, I., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332. [PubMed] [Article] [RRC of NBRP]
Kanegae, Y., Lee, G., Sato, Y., Tanaka, M., Nakai, M., Sakaki, T., Sugano, S., Saito, I., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332. [PubMed] [Article] [RRC of NBRP]
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [PubMed] [Article] [RRC of NBRP]
reference Matsuda, T., Controlled expression of transgenes introduced by in vivo electroporation. Proc. Natl. Acad. Sci. U.S.A. 104 (3): 1027-1032 (2007). PMID 17209010. [PubMed] [Article] [RRC of NBRP]
reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837. [PubMed] [Article] [RRC of NBRP]
user_report Nakahara, T., Activation of NF-κB by Human Papillomavirus 16 E1 Limits E1-Dependent Viral Replication through Degradation of E1. J. Virol. 89 (9): 5040-5059 (2015). PMID 25717108. [PubMed] [Article] [RRC of NBRP]
user_report Sakai, Y., A novel transgenic mouse model carrying human Tribbles related protein 3 (TRB3) gene and its site specific phenotype. Biol. Pharm. Bull., 37 (6): 1068-1074 (2014). PMID 24882419. [PubMed] [Article] [RRC of NBRP]
user_report Nodagashira T., Efficient Gene Transduction in HER2-Expressing Cells by Two Recombinant Adenovirus Vectors with HER2 Promotor and Cre/loxP System. Hirosaki Med. J., 61: 26-34 (2010). [RRC of NBRP]
user_report Kameyama Y., An accumulative site-specific gene integration system using Cre recombinase-mediated cassette exchange. Biotechnol. Bioeng., 105, 1106-1114 (2010). PMID 19998287. [PubMed] [Article] [RRC of NBRP]
user_report Marchetti G., Integrin alpha5beta1 is necessary for regulation of radial migration of cortical neurons during mouse brain development. Eur. J. Neurosci., 31, 399-409, (2010). PMID 20105241. [PubMed] [Article] [RRC of NBRP]
user_report Komatsu, M., Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism. Proc. Natl. Acad. Sci. U S A., 107 (6), 2646-2651 (2010). PMID 20133795. [PubMed] [Article] [RRC of NBRP]
user_report Shimada H., Introduction of tau mutation into cultured Rat1-R12 cells by gene targeting, using recombinant adeno-associated virus vector. Cell. Mol. Neurobiol., 29, 699-705 (2009). PMID 19305950. [PubMed] [Article] [RRC of NBRP]
user_report Long N., Construction of a multi-functional helper-dependent adenovirus based system for cancer gene therapy. Asian Pac. J. Cancer Prev., 10, 939-960 (2009). PMID 20104994. [PubMed] [RRC of NBRP]
user_report Pihkala P., An antigen-mediated selection system for mammalian cells that produce glycosylated single-chain Fv. Biochem. Biophys. Res. Commun., 324, 1165-1172 (2004). PMID 15504336. [PubMed] [Article] [RRC of NBRP]
user_report Kurihara H., Glioma/glioblastoma-specific adenoviral gene expression using the nestin gene regulator. Gene Ther., 7, 686-693 (2000). PMID 10800092. [PubMed] [Article] [RRC of NBRP]