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pxCANCre

A plasmid expressing the recombinase Cre tagged with a nls

Catalog number RDB01675
Resource name pxCANCre
Clone info. A plasmid expressing the recombinase Cre tagged with a nuclear localization signal under the control of CAG promoter
Vector backbone pxCAwt (deleted from pAxCAwt with Sal I) (Plasmid)
Size of vector backbone 6,565 bp
Selectable markers Amp^r
Gene/insert name P1 phage Cre recombinase cDNA
Depositor|Developer Saito, Izumu |
Other clones in our bank

External Database
P1 phage Cre recombinase

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB01675 pxCANCre DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332.
          original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0045e (English text)
          Featured content Dnaconda's recommendation EXR0045j (Japanese text)
          Featured content Control Vectors (English text)
          Featured content Cre-loxP and FLP-FRT System (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14151_A6Ebp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Region: SV40 NLS, insert 5'
          Sequence file: RDB14151_A6Eba.seq check
          >01675_14151_A6Eb_3_pAxCA_F1_C08_08.ab1
              1 TAAGCTATCA TCTGCTACAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG
             61 TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT GATTTATCGA TTTGCAGACC
            121 GTGCATCATG AGCGGCCCTC CAAAAAAGAA GAGAAAGGTA GAAGACCCGG GCGGCCGCAT
            181 GTCCAATTTA CTGACCGTAC ACCAAAATTT GCCTGCATTA CCGGTCGATG CAACGAGTGA
            241 TGAGGTTCGC AAGAACCTGA TGGACATGTT CAGGGATCGC CAGGCGTTTT CTGAGCATAC
            301 CTGGAAAATG CTTCTGTCCG TTTGCCGGTC GTGGGCGGCA TGGTGCAAGT TGAATAACCG
            361 GAAATGGTTT CCCGCAGAAC CTGAAGATGT TCGCGATTAT CTTCTATATC TTCAGGCGCG
            421 CGGTCTGGCA GTAAAAACTA TCCAGCAACA TTTGGGCCAG CTAAACATGC TTCATCGTCG
            481 GTCCGGGCTG CCACGACCAA GTGACAGCAA TGCTGTTTCA CTGGTTATGC GGCGGATCCG
            541 AAAAGAAAAC GTTGATGCCG GTGAACGTGC AAAACAGGCT CTAGCGTTCG AACGCACTGA
            601 TTTCGACCAG GTTCGTTCAC TCATGGAAAA TAGCGATCGC TGCCAGGATA TACGTAATCT
            661 GGCATTTCTG GGGATTGCTT ATAACACCCT GTTACGTATA GCCGAAATTG CCAGGATCAG
            721 GGTTAAAGAT ATCTCACGTA CTGACGGTGG GAGAATGTTA ATCCATATTG GCAGAACGAA
            781 CACGCTGGTT AGCACCGCAG GTGTAGAGAA GGCACTTAGC CTGGGGGTAA CTAAACTGGT
            841 CGAGCGATGG ATTTCCGTCT CTGGTGTAGC TGATGATCCG AATAACTACC TGTTTTGCCG
            901 GGTCAGAAAA AAATGGTGTT GCCGCGCCAT CTGCCCACCA GCCAGCTATC AAACTCGCGC
            961 CCTGGAAAGG ATTTTTTTGA AGCAACCTCA TCGATTGAAT TTTACGGCGC TACGATGGAC
           1021 TCTGGGTTCA GAGGATACCT TGGTCCTGAT CTGACACAGT GGTCCGATGA TCCGGAGCCG
          1081 GCGCCGA
          //
          Primer: pAxCA_R1
          Region: insert 3'
          Sequence file: RDB14151_A6Ebb.seq check
          >01675_14151_A6Eb_3_pAxCA_R1_F08_17.ab1
              1 GGACATGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC CTGAGGAGTG
             61 AATTGATTAT CGATTCTAGA CTAGTTTAAT TAATTTCTAG AGCGCTTAAT GGCTAATCGC
            121 CATCTTCCAG CAGGCGCACC ATTGCCCCTG TTTCACTATC CAGGTTACGG ATATAGTTCA
            181 TGACAATATT TACATTGGTC CAGCCACCAG CTTGCATGAT CTCCGGTATT GAAACTCCAG
            241 CGCGGGCCAT ATCTCGCGCG GCTCCGACAC GGGCACTGTG TCCAGACCAG GCCAGGTATC
            301 TCTGACCAGA GTCATCCTTA GCGCCGTAAA TCAATCGATG AGTTGCTTCA AAAATCCCTT
            361 CCAGGGCGCG AGTTGATAGC TGGCTGGTGG CAGATGGCGC GGCAACACCA TTTTTTCTGA
            421 CCCGGCAAAA CAGGTAGTTA TTCGGATCAT CAGCTACACC AGAGACGGAA ATCCATCGCT
            481 CGACCAGTTT AGTTACCCCC AGGCTAAGTG CCTTCTCTAC ACCTGCGGTG CTAACCAGCG
            541 TTTTCGTTCT GCCAATATGG ATTAACATTC TCCCACCGTC AGTACGTGAG ATATCTTTAA
            601 CCCTGATCCT GGCAATTTCG GCTATACGTA ACAGGGTGTT ATAAGCAATC CCCAGAAATG
            661 CCAGATTACG TATATCCTGG CAGCGATCGC TATTTTCCAT GAGTGAACGA ACCTGGTCGA
            721 AATCAGTGCG TTCGAACGCT AGAGCCTGTT TTGCACGTTC ACCGGCATCA ACGTTTTTCT
            781 TTTCGGATCC GCCGCATAAC CAGTGAAACA GCATTGCTGT CACTTGGTCG TGGCAGCCCG
            841 GACCGACGAT GAAGCATGTT TAGCTGGCCC AAATGTTGCT GGATAGTTTT TACTGCCAGA
            901 CCGCGCGCCT GAAGATATAG AAGATAATCG CGAACATCTT CAGGTTTCTG CGGGAAACCC
            961 ATTTTCCGGT TATTCACTTT GCACCATGAC GCCCACGAAC GGCCAACCGG ACAGAAGGCA
          1021 TTTTGCAGCT ATGCTCAGAA ACGGCTTACG ATGCCATGCA AGCATGATCC AATTC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332.
          original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
          reference Matsuda, T., Controlled expression of transgenes introduced by in vivo electroporation. Proc. Natl. Acad. Sci. U.S.A. 104 (3): 1027-1032 (2007). PMID 17209010.
          reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837.
          user report Nakahara, T., Activation of NF-κB by Human Papillomavirus 16 E1 Limits E1-Dependent Viral Replication through Degradation of E1. J. Virol. 89 (9): 5040-5059 (2015). PMID 25717108.
          user report Sakai, Y., A novel transgenic mouse model carrying human Tribbles related protein 3 (TRB3) gene and its site specific phenotype. Biol. Pharm. Bull., 37 (6): 1068-1074 (2014). PMID 24882419.
          user report Nodagashira T., Efficient Gene Transduction in HER2-Expressing Cells by Two Recombinant Adenovirus Vectors with HER2 Promotor and Cre/loxP System. Hirosaki Med. J., 61: 26-34 (2010).
          user report Kameyama Y., An accumulative site-specific gene integration system using Cre recombinase-mediated cassette exchange. Biotechnol. Bioeng., 105, 1106-1114 (2010). PMID 19998287.
          user report Marchetti G., Integrin alpha5beta1 is necessary for regulation of radial migration of cortical neurons during mouse brain development. Eur. J. Neurosci., 31, 399-409, (2010). PMID 20105241.
          user report Komatsu, M., Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism. Proc. Natl. Acad. Sci. U S A., 107 (6), 2646-2651 (2010). PMID 20133795.
          user report Shimada H., Introduction of tau mutation into cultured Rat1-R12 cells by gene targeting, using recombinant adeno-associated virus vector. Cell. Mol. Neurobiol., 29, 699-705 (2009). PMID 19305950.
          user report Long N., Construction of a multi-functional helper-dependent adenovirus based system for cancer gene therapy. Asian Pac. J. Cancer Prev., 10, 939-960 (2009). PMID 20104994.
          user report Pihkala P., An antigen-mediated selection system for mammalian cells that produce glycosylated single-chain Fv. Biochem. Biophys. Res. Commun., 324, 1165-1172 (2004). PMID 15504336.
          user report Kurihara H., Glioma/glioblastoma-specific adenoviral gene expression using the nestin gene regulator. Gene Ther., 7, 686-693 (2000). PMID 10800092.

          2022.02.09

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