Resource data sheet
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pxCANCre

A plasmid expressing the recombinase Cre tagged with a nls

Catalog number RDB01675
Resource name pxCANCre
Clone info. A plasmid expressing the recombinase Cre tagged with a nuclear localization signal under the control of CAG promoter
Vector backbone pxCAwt (deleted from pAxCAwt with Sal I) (Plasmid)
Size of vector backbone 6,565 bp
Selectable markers Amp^r
Gene/insert name P1 phage Cre recombinase cDNA
Depositor Saito, Izumu |
 
Other clones in our bank P1 phage cre |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01675 pxCANCre DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14151_A6Ebp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB14151_A6Eba.seq
>01675_14151_A6Eb_3_pAxCA_F1_C08_08.ab1
    1 TAAGCTATCA TCTGCTACAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG
   61 TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT GATTTATCGA TTTGCAGACC
  121 GTGCATCATG AGCGGCCCTC CAAAAAAGAA GAGAAAGGTA GAAGACCCGG GCGGCCGCAT
  181 GTCCAATTTA CTGACCGTAC ACCAAAATTT GCCTGCATTA CCGGTCGATG CAACGAGTGA
  241 TGAGGTTCGC AAGAACCTGA TGGACATGTT CAGGGATCGC CAGGCGTTTT CTGAGCATAC
  301 CTGGAAAATG CTTCTGTCCG TTTGCCGGTC GTGGGCGGCA TGGTGCAAGT TGAATAACCG
  361 GAAATGGTTT CCCGCAGAAC CTGAAGATGT TCGCGATTAT CTTCTATATC TTCAGGCGCG
  421 CGGTCTGGCA GTAAAAACTA TCCAGCAACA TTTGGGCCAG CTAAACATGC TTCATCGTCG
  481 GTCCGGGCTG CCACGACCAA GTGACAGCAA TGCTGTTTCA CTGGTTATGC GGCGGATCCG
  541 AAAAGAAAAC GTTGATGCCG GTGAACGTGC AAAACAGGCT CTAGCGTTCG AACGCACTGA
  601 TTTCGACCAG GTTCGTTCAC TCATGGAAAA TAGCGATCGC TGCCAGGATA TACGTAATCT
  661 GGCATTTCTG GGGATTGCTT ATAACACCCT GTTACGTATA GCCGAAATTG CCAGGATCAG
  721 GGTTAAAGAT ATCTCACGTA CTGACGGTGG GAGAATGTTA ATCCATATTG GCAGAACGAA
  781 CACGCTGGTT AGCACCGCAG GTGTAGAGAA GGCACTTAGC CTGGGGGTAA CTAAACTGGT
  841 CGAGCGATGG ATTTCCGTCT CTGGTGTAGC TGATGATCCG AATAACTACC TGTTTTGCCG
  901 GGTCAGAAAA AAATGGTGTT GCCGCGCCAT CTGCCCACCA GCCAGCTATC AAACTCGCGC
  961 CCTGGAAAGG ATTTTTTTGA AGCAACCTCA TCGATTGAAT TTTACGGCGC TACGATGGAC
 1021 TCTGGGTTCA GAGGATACCT TGGTCCTGAT CTGACACAGT GGTCCGATGA TCCGGAGCCG
1081 GCGCCGA
//
Primer: pAxCA_R1 RDB14151_A6Ebb.seq
>01675_14151_A6Eb_3_pAxCA_R1_F08_17.ab1
    1 GGACATGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC CTGAGGAGTG
   61 AATTGATTAT CGATTCTAGA CTAGTTTAAT TAATTTCTAG AGCGCTTAAT GGCTAATCGC
  121 CATCTTCCAG CAGGCGCACC ATTGCCCCTG TTTCACTATC CAGGTTACGG ATATAGTTCA
  181 TGACAATATT TACATTGGTC CAGCCACCAG CTTGCATGAT CTCCGGTATT GAAACTCCAG
  241 CGCGGGCCAT ATCTCGCGCG GCTCCGACAC GGGCACTGTG TCCAGACCAG GCCAGGTATC
  301 TCTGACCAGA GTCATCCTTA GCGCCGTAAA TCAATCGATG AGTTGCTTCA AAAATCCCTT
  361 CCAGGGCGCG AGTTGATAGC TGGCTGGTGG CAGATGGCGC GGCAACACCA TTTTTTCTGA
  421 CCCGGCAAAA CAGGTAGTTA TTCGGATCAT CAGCTACACC AGAGACGGAA ATCCATCGCT
  481 CGACCAGTTT AGTTACCCCC AGGCTAAGTG CCTTCTCTAC ACCTGCGGTG CTAACCAGCG
  541 TTTTCGTTCT GCCAATATGG ATTAACATTC TCCCACCGTC AGTACGTGAG ATATCTTTAA
  601 CCCTGATCCT GGCAATTTCG GCTATACGTA ACAGGGTGTT ATAAGCAATC CCCAGAAATG
  661 CCAGATTACG TATATCCTGG CAGCGATCGC TATTTTCCAT GAGTGAACGA ACCTGGTCGA
  721 AATCAGTGCG TTCGAACGCT AGAGCCTGTT TTGCACGTTC ACCGGCATCA ACGTTTTTCT
  781 TTTCGGATCC GCCGCATAAC CAGTGAAACA GCATTGCTGT CACTTGGTCG TGGCAGCCCG
  841 GACCGACGAT GAAGCATGTT TAGCTGGCCC AAATGTTGCT GGATAGTTTT TACTGCCAGA
  901 CCGCGCGCCT GAAGATATAG AAGATAATCG CGAACATCTT CAGGTTTCTG CGGGAAACCC
  961 ATTTTCCGGT TATTCACTTT GCACCATGAC GCCCACGAAC GGCCAACCGG ACAGAAGGCA
1021 TTTTGCAGCT ATGCTCAGAA ACGGCTTACG ATGCCATGCA AGCATGATCC AATTC
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Link to old catalog RDB01675 (link)
Electronic file Sequence RDB01675z.seq from Depositor

Featured content

Featured content Cre-loxP and FLP-FRT System (English text)
Featured content Dnaconda's recommendation EXR0045e (English text)
Featured content Dnaconda's recommendation EXR0045j (Japanese text)
Featured content Control Vectors (English text)

References

original Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332.
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
reference Matsuda, T., Controlled expression of transgenes introduced by in vivo electroporation. Proc. Natl. Acad. Sci. U.S.A. 104 (3): 1027-1032 (2007). PMID 17209010.
reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-199 (1991). PMID 1660837.
user report Nakahara, T., Activation of NF-κB by Human Papillomavirus 16 E1 Limits E1-Dependent Viral Replication through Degradation of E1. J. Virol. 89 (9): 5040-5059 (2015). PMID 25717108.
user report Sakai, Y., A novel transgenic mouse model carrying human Tribbles related protein 3 (TRB3) gene and its site specific phenotype. Biol. Pharm. Bull., 37 (6): 1068-1074 (2014). PMID 24882419.
user report Nodagashira T., Efficient Gene Transduction in HER2-Expressing Cells by Two Recombinant Adenovirus Vectors with HER2 Promotor and Cre/loxP System. Hirosaki Med. J., 61: 26-34 (2010).
user report Kameyama Y., An accumulative site-specific gene integration system using Cre recombinase-mediated cassette exchange. Biotechnol. Bioeng., 105, 1106-1114 (2010). PMID 19998287.
user report Marchetti G., Integrin alpha5beta1 is necessary for regulation of radial migration of cortical neurons during mouse brain development. Eur. J. Neurosci., 31, 399-409, (2010). PMID 20105241.
user report Komatsu, M., Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism. Proc. Natl. Acad. Sci. U S A., 107 (6), 2646-2651 (2010). PMID 20133795.
user report Shimada H., Introduction of tau mutation into cultured Rat1-R12 cells by gene targeting, using recombinant adeno-associated virus vector. Cell. Mol. Neurobiol., 29, 699-705 (2009). PMID 19305950.
user report Long N., Construction of a multi-functional helper-dependent adenovirus based system for cancer gene therapy. Asian Pac. J. Cancer Prev., 10, 939-960 (2009). PMID 20104994.
user report Pihkala P., An antigen-mediated selection system for mammalian cells that produce glycosylated single-chain Fv. Biochem. Biophys. Res. Commun., 324, 1165-1172 (2004). PMID 15504336.
user report Kurihara H., Glioma/glioblastoma-specific adenoviral gene expression using the nestin gene regulator. Gene Ther., 7, 686-693 (2000). PMID 10800092.

2017.10.17

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