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pCALNLw

A cassette to introduce cDNA into mammalian chromosome

Catalog number RDB01674
Resource name pCALNLw
Clone info. Please use RDB1862 pCALNL5 instead of this clone. A cassette to introduce cDNA into mammalian chromosome under OFF-expression condition, which can be switched ON by the Cre-expressing adenovirus.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 6,071 bp
Selectable markers Amp^r (E. coli)
Gene/insert name E. coli neo Genomic DNA
Depositor Saito, Izumu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
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RDB01674 pCALNLw DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01674.gif from Depositor

Featured content

Featured content Cre-loxP and FLP-FRT System (English text)
Featured content Control Vectors (English text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

References

original Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332.
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

User reports and related articles (go to bottom)


Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

References

original Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332.
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-199 (1991). PMID 1660837.
user report Ueda S., Ductal origin of pancreatic adenocarcinomas induced by conditional activation of a human Ha-ras oncogene in rat pancreas. Carcinogenesis, 27, 2497-2510 (2006). PMID 16774944.

2018.12.11

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