Resource data sheet
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pSTY81-20 (#RDB01638)

Plasmid clone of E. coli melA and melB genomic DNA

Alternative name E. coli melA and melB DNA
Clone info. Plasmid clone of E. coli melA and melB genomic DNA
Vector backbone pBR322 (Plasmid)
Size of vector backbone 4,361 bp
Selectable markers Tet^r
Gene/insert name E. coli MelA and melB Genomic DNA
Depositor|Developer Tsuchiya, Tomofusa |
 
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Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB01638 pSTY81-20 Under QC test. Please contact us. DNA solution Please contact us.

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pSTY81-20 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01638).

Reference section:

Hanatani, M., Yazyu, H., Shiota-Niiya, S., Moriyama, Y., Kanazawa, H., Futai, M., Tsuchiya, T., Physical and genetic characterization of the melibiose operon and identification of the gene products in Escherichia coli. J. Biol. Chem., 259, 1807-1812 (1984). PMID 6319412.
Yazyu, H., Shiota-Niiya, S., Shimamoto, T., Kanazawa, H., Futai, M., Tsuchiya, T., Nucleotide sequence of the melB gene and characteristics of deduced amino acid sequence of the melibiose carrier in Escherichia coli. J. Biol. Chem., 259, 4320-4326 (1984). PMID 6323466.
Tsuchiya, T., Ottina, K., Moriyama, Y., Newman, M.J., Wilson, T.H., Solubilization and reconstitution of the melibiose carrier from a plasmid-carrying strain of Escherichia coli. J. Biol. Chem., 257, 5125-5128 (1982). PMID 7040393.

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Hanatani, M., Physical and genetic characterization of the melibiose operon and identification of the gene products in Escherichia coli. J. Biol. Chem., 259, 1807-1812 (1984). PMID 6319412.
original Yazyu, H., Nucleotide sequence of the melB gene and characteristics of deduced amino acid sequence of the melibiose carrier in Escherichia coli. J. Biol. Chem., 259, 4320-4326 (1984). PMID 6323466.
original Tsuchiya, T., Solubilization and reconstitution of the melibiose carrier from a plasmid-carrying strain of Escherichia coli. J. Biol. Chem., 257, 5125-5128 (1982). PMID 7040393.