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pAEG. BE2-2 (#RDB01634)

Mouse acidic epididymal glycoprotein cDNA

Alternative name AEG
Clone info. Acidic epididymal glycoprotein (AEG) cDNA from mouse epididymis (C57BL/6J)
Vector backbone pBluescript SK (-) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Mouse Acidic epididymal glycoprotein 1 (Aeg1) cDNA
Reference of insert sequence M92849 (DDBJ accession number)
Depositor|Developer Kasahara, Masanori | Mizuki, Nobuyuki |
 
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01634 pAEG. BE2-2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAEG. BE2-2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01634).

Reference section:

Kasahara, M., Hayashi, M., Yoshida, M.C., Nadeau, J.H., Fujimoto, S., Ishibashi, T., Mapping of acidic epididymal glycoprotein (Aeg) genes to mouse chromosome 17. Mammalian Genome, 6, 52-54 (1995). PMID 7719028.
Mizuki, N., Kasahara, M., Mouse submandibular glands express an androgen-regulated transcript encoding an acidic epididymal glycoprotein-like molecule. Mol. Cell. Endocrinol., 89, 25-32 (1992). PMID 1301383.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16015_A8Hrp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2
Sequence file: RDB16015_A8Hra.seq check
>01634_16015_A8Hr_Reverse2_B11_05_ABI24.ab1
    1 AGGCCAGGCA TACGCCAAGC TCGAAATTAA CCCTCACTAA AGGGAACAAA AGCTGGAGCT
   61 CCACCGCGGT GGCGGCCGCT CTAGAACTAG TGGATCCCCC GGGCTGCAGG AATTCGGCAC
  121 GAGGCCAGCA CCATGGCATT AATGCTTGTG CTGTTCTTCT TGGCTGCTGT ACTGCCCCCA
  181 TCCCTTCTTC AAGATAGCTC TCAGGAAAAT CGTCTTGAGA AACTTTCAAC CACTAAAATG
  241 TCAGTCCAAG AAGAGATTGT AAGCAAGCAC AACCAATTGA GACGAATGGT TTCTCCATCT
  301 GGCAGTGACT TACTAAAAAT GGAATGGAAC TATGATGCTC AAGTGAATGC TCAGCAATGG
  361 GCAGACAAGT GTACATTCAG TCACAGTCCT ATAGAACTCA GGACAACTAA TTTAAGATGT
  421 GGGGAGAATT TGTTCATGTC ATCTTACCTT GCATCATGGT CTTCTGCAAT CCAAGGATGG
  481 TATAATGAAT ACAAAGATCT TACATATGAT GTTGGCCCAA AGCAACCTGA TAGTGTGGTT
  541 GGACATTATA CTCAGGTTGT TTGGAACTCA ACTTTCCAAG TTGCATGTGG AGTTGCTGAA
  601 TGCCCTAAAA ATCCACTGAG ATACTATTAT GTTTGTCACT ATTGTCCTGT TGGCAATTAT
  661 CAAGGAAGGC TATACACACC TTACACTGCA GGAGAACCGT GTGCCAGTTG TCCTGATCAC
  721 TGTGAAGATG GGCTATGCAC CAATAGTTGT GGACATGAAG ATAAGTATAC TAACTGTAAA
  781 TATCTGAAGA AGATGCTATC CTGTGAACAT GAACTTCTTA AAAAAAGGTT GCAAAGCTAC
  841 ATGCCTCTGT GAAGGCAAAA TTCACTAAAT TTCCTGTACT CGTAGCCAGG ACCATGTAGA
  901 GAAAGCTCAT ACTCTCTAGT TAGGCTTATC ACATCCCACC AAGAAAGGTA TAGATTTAGG
  961 ACATTGAAAT AATTTCCAGA TAGTAAAGAT TCTGTTTCTT CTTCTATTTC TTTCTATTTT
 1021 TACAGAAATC ATTTACCCCC AATATTTTTA AATACAAATT TGATATACCC TTTGGTACTT
1081 TGGACATATG AAATCTGATG ACACCATTTT TTCC
//
Primer: pUC-M13_-100
Sequence file: RDB16015_A8Hrb.seq check
>01634_16015_A8Hr_pUC-M13_-100_A11_02_ABI24.ab1
    1 GACTGTCAGG CGATTAGTTG GGTACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA
   61 CGGCCAGTGA ATTGTAATAC GACTCACTAT AGGGCGAATT GGGTACCGGG CCCCCCCTCG
  121 AGTTTTTTTT TTTTTTTTTT AACATGCCAA GATTAAGATT TATTATTTTT CTAAAGCAAC
  181 AAAGGTCATT GTCCCCTGAA CAAGCAAACT ATAAATTCTG CTCGTTAAGG TACATTTTAT
  241 ATCATTCTTT AAAAAAACTA AAATGTAAAT AGTAGTTTAG AATTTAAACC ACTGATTATG
  301 ATGAATTTGA AGAATAAGGA ATAGTGAGAA AGAAAATAAA AGACGATTAA TGTGATTTTC
  361 TCCAGAGAGA GTAAACTAGG TAGAGCTAGA AAAGATGACT GACCATTATA CAGGAACTAT
  421 ATGTTTTCTA TTCCTTTACT TCATTGTGCT CTTGGAACAT TAGTCATATG AGTTTTAGTG
  481 ACTTTAGTCA TACAGACAAT GTATAATCAT GGGCTAGACT TGACTCCGAA AATGTGTCAC
  541 AGATTTCATA TGTCAAGGTA CAAAGGTATT ATCAAATTTG TTATTTTAAA ATATTTGGGG
  601 TAAATGATTT CTGTAAAATA GAAAGAAATA GAAGAAGAAA CAGAATCTTT ACTATCTGGA
  661 ATTATTTCAA TGTCCTAAAT CTATACTTTC TTGGTGGGAT GTGATAAGCC TAACTAGAGA
  721 GTATGAGCTT TCTCTACATG GTCCTGGCTA CGAGTACAGG AAATTTAGTG AATTTTGCCT
  781 TCACAGAGGC ATGTAGCTTT GCAACCTTTT TTAAGAAGTT CATGTTCACA GGATAGCATC
  841 TTCTTCAGAT ATTTACAGTT AGTATACTTA TCTTCATGTC CACAACTATT GGTGCATAGC
  901 CCATCTTCAC AGTGATCAGG ACAACTGGCA CACGGGTTCT CCTGCAGTGT AAAGGTGTGT
  961 ATAGCCTTCC CTTGATAATT GCCCAACAGG ACAAATAGTG GACAAACATA ATAGTATCTC
 1021 AGTGGGATTT TTTTAGGGCA TTCAGCTACT CCTACATGCT ACCTTTGGAA AGGTTGAGTT
 1081 CCCAACGACT TGAAGTATAA TGGTTCCTAC TAACTATCAG CTGCTAGGCC ACATCATATG
1141 TGTA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kasahara, M., Mapping of acidic epididymal glycoprotein (Aeg) genes to mouse chromosome 17. Mammalian Genome, 6, 52-54 (1995). PMID 7719028.
original Mizuki, N., Mouse submandibular glands express an androgen-regulated transcript encoding an acidic epididymal glycoprotein-like molecule. Mol. Cell. Endocrinol., 89, 25-32 (1992). PMID 1301383.