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pAxCACD

Shuttle vector to generate rAd expressing E. coli cytosine deaminase

Catalog number RDB01591
Resource name pAxCACD
Alternative name pAdex1CACD
Clone info. Shuttle vector to generate recombinant adenovirus vector expressing E. coli cytosine deaminase gene regulated by CA promoter.
Produce adenovirus by COS-TPC method.
Vector backbone pAxcw (Cosmid, use packaging extracts for transformation)
Size of vector backbone 42.5 kb
Selectable markers Amp^r
Gene/insert name E. coli cytosine deaminase Genomic DNA
Depositor Hamada, Hirofumi |
 
Other clones in our bank E. coli codA (KEGG orthology K01485) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Cancer Gene Ther., 7, 1015-1022 (2000).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Ordering Please visit Information of Request for Distribution. 
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Material Transfer Agreement (MTA) [Word]
Remarks Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB01591 pAxCACD Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01591.gif from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Suicide gene therapy (English text)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

user report Hamaji, Y., Strong enhancement of recombinant cytosine deaminase activity in Bifidobacterium longum for tumor-targeting enzyme/prodrug therapy. Biosci. Biotechnol. Biochem., 71, 874-883 (2007). PMID 17420581.
user report Nakamura T., Cloned cytosine deaminase gene expression of Bifidobacterium longum and application to enzyme/pro-drug therapy of hypoxic solid tumors. Biosci. Biotechnol. Biochem., 66, 2362-2366 (2002). PMID 12506973.

2019.09.18

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