RDB01566 - AxCAmB7

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AxCAmB7 (#RDB01566)

Recombinant adenovirus expressing mouse B7 (CD80) gene

Alternative name	Adex1CAmB7
Clone info.	Recombinant adenovirus expressing mouse B7 (CD80) gene regulated by CAG promoter.
Vector backbone	Adex1 (Adenovirus)
Size of vector backbone	-
Growth remarks	5 percent FCS is fine.
Gene/insert name	Mouse CD80 (B7-1) cDNA
Depositor\|Developer	Hamada, Hirofumi \|

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Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

Remarks Please ensure RCAs are not in stocks prior to use of recombinant adenoviruses. (https://brc.riken.jp/dna/GRP0046e)

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks	Please ensure RCAs are not in stocks prior to use of recombinant adenoviruses. (https://brc.riken.jp/dna/GRP0046e)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 遺伝子組換え生物の受入れ確認書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。
備考	【追加提供依頼書】受入れ確認書(書式J) 実験に使用する前に、タイターを上げた試料中にRCAが混入していないことを確認してください。(https://brc.riken.jp/dna/GRP0046j) 組換え体提供にかかる書式が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01566	AxCAmB7	Virus, lysate of infected cell culture	JPY 32,340 plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The AxCAmB7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01566).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB01566_0003308p2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB01566_0003308a.seq
>1566_1_0003308_pAxCA_F1_E01_05_ABI08.ab1 1 CGTTTCCTGC CTATAATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC 61 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTAGA CGGCATCGCA GCTTGGATAC 121 ACGCCGCCCA CGTGAAGGCT GCCGACCCCG GGGGTGGACC ATCCTCTAGA CTGCCATGGC 181 TTGCAATTGT CAGTTGATGC AGGATACACC ACTCCTCAAG TTTCCATGTC CAAGGCTCAT 241 TCTTCTCTTT GTGCTGCTGA TTCGTCTTTC ACAAGTGTCT TCAGATGTTG ATGAACAACT 301 GTCCAAGTCA GTGAAAGATA AGGTATTGCT GCCTTGCCGT TACAACTCTC CTCATGAAGA 361 TGAGTCTGAA GACCGAATCT ACTGGCAAAA ACATGACAAA GTGGTGCTGT CTGTCATTGC 421 TGGGAAACTA AAAGTGTGGC CCGAGTATAA GAACCGGACT TTATATGACA ACACTACCTA 481 CTCTCTTATC ATCCTGGGCC TGGTCCTTTC AGACCGGGGC ACATACAGCT GTGTCGTTCA 541 AAAGAAGGAA AGAGGAACGT ATGAAGTTAA ACACTTGGCT TTAGTAAAGT TGTCCATCAA 601 AGCTGACTTC TCTACCCCCA ACATAACTGA GTCTGGAAAC CCATCTGCAG ACACTAAAAG 661 GATTACCTGC TTTGCTTCCG GGGGTTTCCC AAAGCCTCGC TTCTCTTGGT TGGAAAATGG 721 AAGAGAATTA CCTGGCATCA ATACGACAAT TTCCCAGGAT CCTGAATCTG AATTGTACAC 781 CATTAGTAGC CAAACTAGAT TTCAATACGA CTCGCAACCA CACCATTAAG TGTCTCATTA 841 AATATGGAGA TGCTCACGTG TCAGAGGACT TCACCTGGGA AAAAACCCCC AGAAAGACCC 901 TCCTGATAGC AAGGAACACA CTTTGTGCTC TTTGGGGCAG GATTCGGCGC AATAAATAAC 961 AGTCGTCGTC ATCGTTGTCA TCATCAAATG CCTTCAGTAA GCACAGAAGC TGGTTTTCCG 1021 AGGAAATTGG AGGAAAGGCA GGAGAGACAA ACAACACCCT TACACTTCCG GGCCCTGGAA 1081 CGGAAGGCGT TTAGACATGG ACCAAACGGG GCGTTCCGTT TGGGATCCCG GAATTAGTCC 1141 CAGTTTGTGT TAAACTACTA GTGGACAAAT ACCAGCCACG TCAAAGAGTT CTCAAGGCTC 1201 TGCCCCTCAT CTGCCAGAAA ACGGCGGGCC GGACGTGAGT CAT //
Primer: pAxCA_R1 Sequence file: RDB01566_0003308b.seq
>1566_1_0003308_pAxCA_R1_F01_06_ABI08.ab1 1 GCAATAGGAG GGAGCCATGG GCCACACCAG CCACCACCTT CTGATAGGCA GCCTGCACCT 61 GAGGAGTGAA TTATCCTGTC TTTAACAAAT TGGACTAATC CGGATCTAAA GGAAGACGGT 121 CTGTTCAGCT AATGCTTCTT CAGGCCCGAA GGTAAGGCTG TTGTTTGTTT CTCTGCTTGC 181 CTCATTTCTT CTGAAACAGC TTCTGTGCTT ACAGAAGCAT TTGATGATGA CAACGATGAC 241 GACGACTGTT ATTACTGCGC CGAATCCTGC CCCAAAGAGC ACAAGTGTGT TCTTGCTATC 301 AGGAGGGTCT TCTGGGGGTT TTTCCCAGGT GAAGTCCTCT GACACGTGAG CATCTCCATA 361 TTTAATGAGA CACTTAATGG TGTGGTTGCG AGTCGTATTG AAATCTAGTT GGCTACTAAT 421 GGTGTACAAT TCAGATTCAG GATCCTGGGA AATTGTCGTA TTGATGCCAG GTAATTCTCT 481 TCCATTTTCC AACCAAGAGA AGCGAGGCTT TGGGAAACCC CCGGAAGCAA AGCAGGTAAT 541 CCTTTTAGTG TCTGCAGATG GGTTTCCAGA CTCAGTTATG TTGGGGGTAG AGAAGTCAGC 601 TTTGATGGAC AACTTTACTA AAGCCAAGTG TTTAACTTCA TACGTTCCTC TTTCCTTCTT 661 TTGAACGACA CAGCTGTATG TGCCCCGGTC TGAAAGGACC AGGCCCAGGA TGATAAGAGA 721 GTAGGTAGTG TTGTCATATA AAAGTCCGGT TCTTATACTC GGGCCACACT TTTTAGTTTT 781 CCCAGCAATG ACAGACAGCA CCACTTTGTC ATGTTTTTGC CAGTAGATTC GGTCTTTCAG 841 ACTCATCTTC CTGAGGAGAG TTGTAACGGG CAAGGGCGGC ATACCTTATC TTTCACTGAC 901 TTGGAACAGG TTTGTTCATC AACATTCTTG AAGACCTTTG TGAAAGACGA ATCAACCAGC 961 ACAAAAGAGA AAGAAAAGAA GCCTTGGAAC ATGGGAAACT TGAGGAAGTG GTGGTATTCC 1021 TGGAATCAAC TGACAATTGC ATGACATGAG TAGATCTAAA GGATGAGTCA ACCCAGGGCT 1081 CCGCCCGCGT TGCGCCTGGA GACCGACGTT GTTGCCAAAG CCTGGCTGAT TGCCCTGTCT 1141 TAATATCGAT TTTTGGCCTA ATATTAGTCT TGTAATGTAA CAGTCCAACA AAGTAAGACC 1201 CGAAAGACTA CATGTTCTAC TCACTCCCCA AGCGTAAAGC CTTGCTATGC CTGA //