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pBS-F

Sendai virus F protein

Catalog number RDB01543
Resource name pBS-F
Alternative name Sendai virus F protein
Clone info. Sendai virus F protein
Vector backbone pBluescript II SK (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Sendai virus Sendai virus F glycoprotein cDNA
Depositor|Developer Taira, Hideharu | Shioda, Tatsuo |
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External Database
Sendai virus Sendai virus F glycoprotein

            Reference sequence
              

            Distribution information

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            Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for not-for-profit research in the not-for-profit organization.
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            提供条件 本件リソースは非営利機関にのみ提供し、非営利研究にのみ利用することができる。
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            RDB01543 pBS-F DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Remarks, protocol and/or map (gif) RDB01543.gif

            Original reference

            original Taira, H., Construction of expression plasmids for the fusion protein of Sendai virus, and their expression in E. coli cells and eucaryotic cells. FEBS Lett., 262, 39-41 (1990). PMID 2156733.
            original Shioda, T., Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequences of the F, HN and L proteins. Nucleic Acids Res., 14, 1545-1563 (1986). PMID 3005975.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13248_A5Gs.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13_-40
            Sequence file: RDB13248_A5Gsa.seq check
            >01543_13248_A5Gs_M13(-40)_A04_01.ab1
                1 AGTCCAGTTA CGCGCGTATA CGACTCACTA TAGGGCGAAT TGGGTACCGG GCCCCCCCTC
               61 GAGGTCGACG GTATCGATAA GCTTGGGCTT GGGAAACATG ACAGCATATA TCCAGAGATC
              121 ACAGTGCATC TCAACATCAC TACTGGTTGT TCTCACCACA TTGGTCTCGT GTCAGATTCC
              181 CAGGGATAGG CTCTCTAACA TAGGGGTCAT AGTCGATGAA GGGAAATCAC TGAAGATAGC
              241 TGGATCCCAC GAATCGAGGT ACATAGTACT GAGTCTAGTT CCGGGGGTAG ACTTTGAGAA
              301 TGGGTGCGGA ACAGCCCAGG TTATCCAGTA CAAGAGCCTA CTGAACAGGC TGTTAATCCC
              361 ATTGAGGGAT GCCTTAGATC TTCAGGAGGC TCTGATAACT GTCACCAATG ATACGACGCA
              421 AAATGCCGGT GCTCCCCAGT CGAGATTCTT CGGTGCTGTG ATTGGTACTA TCGCACTTGG
              481 AGTGGCGACA TCAGCACAAA TCACCGCAGG GATTGCACTA GCCGAAGCGA GGGAGGCCAA
              541 AAGAGACATA GCGCTCATCA AAGAATCGAT GACAAAAACA CACAAGTCTA TAGAACTGCT
              601 GCAAAACGCT GTGGGGGAAC AAATTCTTGC TCTAAAGACA CTCCAGGATT TCGTGAATGA
              661 TGAGATCAAA CCCGCAATAA GCGAATTAGG CTGTGAGACT GCTGCCTTAA GACTGGGTAT
              721 AAAATTGACA CAGCATTACT CCGAGCTGTT AACTGCGTTC GGCTCGAATT TCGGAACCAT
              781 CGGAGAGAAG AGCCTCACGC TGCAGGCGCT GTCTTCACTT TACTCTGCTA ACATTACTGA
              841 GATTATGACC ACAATCAGGA CAGGGCAGTC TAACATCTAT GATGTCATTT ATACAGAACA
              901 GATCAAAGGA ACGGTGATAG ATGTGGATCT AGAGAGATAC ATGGTCACCC TGTCTGTGAA
              961 GATCCCTATT CTTTCTGAAG TCCCAGGTGT GCTCATACAC AAAGGCATCA TCTATTTCTT
             1021 TACAACATAG ACGGGGAGGG AATGGTTATG TGACTGTCCC CAGCCCATAT ACTCAGTCGT
             1081 GCTTCTTTTC TTAAGGGGGT GCAAGAACTA ACGAATGTGT GAGTCCAGAT TGACCTATAA
            1141 TATTGCCCCC AAGGGGAATT CCCCGG
            //
            Primer: Reverse2
            Sequence file: RDB13248_A5Gsb.seq check
            >01543_13248_A5Gs_Reverse2_B04_04.ab1
                1 AGTACTGATT ACGCCAGCGC GCATTAACCC TCACTAAAGG GAACAAAAGC TGGAGCTCCA
               61 CCGCGGTGGC GGCCGCTCTA GAACTAGTGG ATCCCCCGGG CTGCAGGAAT TCGATATCAA
              121 GCTTGGGGAT CTCAACGGAT ACTATGCCTG CTTTACAAGA CATCTGATAA TGGTCGTGAT
              181 CATCTTTTCT CAGCCATTGC ATCAAACCCA CCGTTTGTGT ACATATGTCT GATCTTCGGC
              241 TCTAATGTGT ATGTGTCCCT CGGTATACGG TCATCTGGAT TACCCATTAG CATTGACCTT
              301 CTGAGTCTAT AAAGCACGAT GATGATCACT ATAATGACCA CCAATATTAC GACCATAACT
              361 ACTATGATCG TAATCACAGT CTCTCTTGAG TTGTACCATC TACCTACCTC CGAGAGGATT
              421 TTCCGTGCTT TCTCAAGCTC AGCCTTAGAG TCTTGCAAGA AATTCGTAGC ATCAGCAAGG
              481 TTGAGAGAAA TATCAACGGG TCTGATAGCA ATTGCAGGAC CGACTGTCAA GTTCTGGACC
              541 CCCCAAGTGG CATCGTGCCC TCTCCGGTTA GCATACAATT CTACCCCATT GACACCTATA
              601 AGACCACAGT TGTCATGGGT TAGGAATACT ACACCTTTAG AGCGATCCTG ACTGATTGGT
              661 CTTCGGCCTG TCCCGCAGGT ACATGTGGAT GCTATGCAGT TAGCAACAAC GCCCCCATTC
              721 ACAAAAGCAA ACTTGGGGAT AAGGCTGTCC ACAACTTTTG TGACAGGACA CCTTGTTGTG
              781 TCCCCCAGGA TACACTTTTG CTGGCTGTCA GGTATCAGTT GTGCGGGATC CCTGGGGCAT
              841 ATATAGGTCA ATCTGGACTC AACACAATCG GTTATGTCTG CACCCCCTAA GAAAGAAGCA
              901 CGACTGAGTA TATGGCTGGG GGACAGTCAC ATACCATTCC TCCCCGTCTA TGTTGTAAGA
              961 AATAGATGAT GCCTTGTGTA TGAGCACACC TGGGGACTTC AGAAAGAATA GGGATCTTCA
             1021 CAGACAGGGT GACCATGTAT CTCTCTAGAT CCACATCTAT CACCGATCGT TTTGATCTGC
             1081 TCTGTATAAA TGACATCATA GATGGTAGGA CCTTGCCCTT GATCCCTTGC ATGGTGGGCT
            1141 TCATAATTCT CTCTCAG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Taira, H., Construction of expression plasmids for the fusion protein of Sendai virus, and their expression in E. coli cells and eucaryotic cells. FEBS Lett., 262, 39-41 (1990). PMID 2156733.
            original Shioda, T., Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequences of the F, HN and L proteins. Nucleic Acids Res., 14, 1545-1563 (1986). PMID 3005975.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl