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pBS-F (#RDB01543)

Sendai virus F protein

Alternative name Sendai virus F protein
Clone info. Sendai virus F protein
Vector backbone pBluescript II SK (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Sendai virus Sendai virus F glycoprotein cDNA
Depositor|Developer Taira, Hideharu | Shioda, Tatsuo |
 
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Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for not-for-profit research in the not-for-profit organization.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 本件リソースは非営利機関にのみ提供し、非営利研究にのみ利用することができる。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01543 pBS-F DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBS-F was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01543).

Reference section:

Taira, H., Ranin, L., Iwasaki, K., Construction of expression plasmids for the fusion protein of Sendai virus, and their expression in E. coli cells and eucaryotic cells. FEBS Lett., 262, 39-41 (1990). PMID 2156733.
Shioda, T., Iwasaki, K., Shibuta, H., Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequences of the F, HN and L proteins. Nucleic Acids Res., 14, 1545-1563 (1986). PMID 3005975. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13248_A5Gs.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB13248_A5Gsa.seq check
>01543_13248_A5Gs_M13(-40)_A04_01.ab1
    1 AGTCCAGTTA CGCGCGTATA CGACTCACTA TAGGGCGAAT TGGGTACCGG GCCCCCCCTC
   61 GAGGTCGACG GTATCGATAA GCTTGGGCTT GGGAAACATG ACAGCATATA TCCAGAGATC
  121 ACAGTGCATC TCAACATCAC TACTGGTTGT TCTCACCACA TTGGTCTCGT GTCAGATTCC
  181 CAGGGATAGG CTCTCTAACA TAGGGGTCAT AGTCGATGAA GGGAAATCAC TGAAGATAGC
  241 TGGATCCCAC GAATCGAGGT ACATAGTACT GAGTCTAGTT CCGGGGGTAG ACTTTGAGAA
  301 TGGGTGCGGA ACAGCCCAGG TTATCCAGTA CAAGAGCCTA CTGAACAGGC TGTTAATCCC
  361 ATTGAGGGAT GCCTTAGATC TTCAGGAGGC TCTGATAACT GTCACCAATG ATACGACGCA
  421 AAATGCCGGT GCTCCCCAGT CGAGATTCTT CGGTGCTGTG ATTGGTACTA TCGCACTTGG
  481 AGTGGCGACA TCAGCACAAA TCACCGCAGG GATTGCACTA GCCGAAGCGA GGGAGGCCAA
  541 AAGAGACATA GCGCTCATCA AAGAATCGAT GACAAAAACA CACAAGTCTA TAGAACTGCT
  601 GCAAAACGCT GTGGGGGAAC AAATTCTTGC TCTAAAGACA CTCCAGGATT TCGTGAATGA
  661 TGAGATCAAA CCCGCAATAA GCGAATTAGG CTGTGAGACT GCTGCCTTAA GACTGGGTAT
  721 AAAATTGACA CAGCATTACT CCGAGCTGTT AACTGCGTTC GGCTCGAATT TCGGAACCAT
  781 CGGAGAGAAG AGCCTCACGC TGCAGGCGCT GTCTTCACTT TACTCTGCTA ACATTACTGA
  841 GATTATGACC ACAATCAGGA CAGGGCAGTC TAACATCTAT GATGTCATTT ATACAGAACA
  901 GATCAAAGGA ACGGTGATAG ATGTGGATCT AGAGAGATAC ATGGTCACCC TGTCTGTGAA
  961 GATCCCTATT CTTTCTGAAG TCCCAGGTGT GCTCATACAC AAAGGCATCA TCTATTTCTT
 1021 TACAACATAG ACGGGGAGGG AATGGTTATG TGACTGTCCC CAGCCCATAT ACTCAGTCGT
 1081 GCTTCTTTTC TTAAGGGGGT GCAAGAACTA ACGAATGTGT GAGTCCAGAT TGACCTATAA
1141 TATTGCCCCC AAGGGGAATT CCCCGG
//
Primer: Reverse2
Sequence file: RDB13248_A5Gsb.seq check
>01543_13248_A5Gs_Reverse2_B04_04.ab1
    1 AGTACTGATT ACGCCAGCGC GCATTAACCC TCACTAAAGG GAACAAAAGC TGGAGCTCCA
   61 CCGCGGTGGC GGCCGCTCTA GAACTAGTGG ATCCCCCGGG CTGCAGGAAT TCGATATCAA
  121 GCTTGGGGAT CTCAACGGAT ACTATGCCTG CTTTACAAGA CATCTGATAA TGGTCGTGAT
  181 CATCTTTTCT CAGCCATTGC ATCAAACCCA CCGTTTGTGT ACATATGTCT GATCTTCGGC
  241 TCTAATGTGT ATGTGTCCCT CGGTATACGG TCATCTGGAT TACCCATTAG CATTGACCTT
  301 CTGAGTCTAT AAAGCACGAT GATGATCACT ATAATGACCA CCAATATTAC GACCATAACT
  361 ACTATGATCG TAATCACAGT CTCTCTTGAG TTGTACCATC TACCTACCTC CGAGAGGATT
  421 TTCCGTGCTT TCTCAAGCTC AGCCTTAGAG TCTTGCAAGA AATTCGTAGC ATCAGCAAGG
  481 TTGAGAGAAA TATCAACGGG TCTGATAGCA ATTGCAGGAC CGACTGTCAA GTTCTGGACC
  541 CCCCAAGTGG CATCGTGCCC TCTCCGGTTA GCATACAATT CTACCCCATT GACACCTATA
  601 AGACCACAGT TGTCATGGGT TAGGAATACT ACACCTTTAG AGCGATCCTG ACTGATTGGT
  661 CTTCGGCCTG TCCCGCAGGT ACATGTGGAT GCTATGCAGT TAGCAACAAC GCCCCCATTC
  721 ACAAAAGCAA ACTTGGGGAT AAGGCTGTCC ACAACTTTTG TGACAGGACA CCTTGTTGTG
  781 TCCCCCAGGA TACACTTTTG CTGGCTGTCA GGTATCAGTT GTGCGGGATC CCTGGGGCAT
  841 ATATAGGTCA ATCTGGACTC AACACAATCG GTTATGTCTG CACCCCCTAA GAAAGAAGCA
  901 CGACTGAGTA TATGGCTGGG GGACAGTCAC ATACCATTCC TCCCCGTCTA TGTTGTAAGA
  961 AATAGATGAT GCCTTGTGTA TGAGCACACC TGGGGACTTC AGAAAGAATA GGGATCTTCA
 1021 CAGACAGGGT GACCATGTAT CTCTCTAGAT CCACATCTAT CACCGATCGT TTTGATCTGC
 1081 TCTGTATAAA TGACATCATA GATGGTAGGA CCTTGCCCTT GATCCCTTGC ATGGTGGGCT
1141 TCATAATTCT CTCTCAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Taira, H., Construction of expression plasmids for the fusion protein of Sendai virus, and their expression in E. coli cells and eucaryotic cells. FEBS Lett., 262, 39-41 (1990). PMID 2156733.
original Shioda, T., Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequences of the F, HN and L proteins. Nucleic Acids Res., 14, 1545-1563 (1986). PMID 3005975. [link to RRC of NBRP]