RDB01542 - pBS-HN

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pBS-HN (#RDB01542)

Sendai virus HN protein

Alternative name	Sendai virus HN protein
Clone info.	Sendai virus HN protein
Vector backbone	pBluescript SK (+) (Plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Amp^r
Gene/insert name	Sendai virus Sendai virus HN glycoprotein cDNA
Depositor\|Developer	Taira, Hideharu \| Shioda, Tatsuo \|

Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for not-for-profit research in the not-for-profit organization.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for not-for-profit research in the not-for-profit organization.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件リソースは非営利機関にのみ提供し、非営利研究にのみ利用することができる。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01542	pBS-HN	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBS-HN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01542).

Reference section:

Taira, H., Ranin, L., Iwasaki, K., Construction of expression plasmids for the fusion protein of Sendai virus, and their expression in E. coli cells and eucaryotic cells. FEBS Lett., 262, 39-41 (1990). PMID 2156733.

Shioda, T., Iwasaki, K., Shibuta, H., Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequences of the F, HN and L proteins. Nucleic Acids Res., 14, 1545-1563 (1986). PMID 3005975. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13249_A5D3p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13(-40) () Sequence file: RDB13249_A5D3a.seq
>01542_13249_A5D3_3_M13(-40)_E02_14.ab1 1 AGTCAAGGGA GCGCGCGTAT ACGACTCACT ATAGGGCGAA TTGGGTACCG GGCCCCCCCT 61 CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCCTG CAGCCCGGGG GATCCCCGGT 121 ACTTTAGCTT TCACCTCAAA CAAGCACAGA TCATGGATGG TGATAGGGGC AAACGTGACT 181 CGTACTGGTC TACTTCTCCT AGTGGTAGCA CCACAAAACC AGCATCAGGT TGGGAGAGGT 241 CAAGTAAAGC CGACACATGG TTGCTGATTC TCTCATTCAC CCAGTGGGCT TTGTCAATTG 301 CCACAGTGAT CATCTGTATC ATAATTTCTG CTAGACAAGG GTATAGTATG AAAGAGTACT 361 CAATGACTGT AGAGGCATTG AACATGAGCA GCAGGGAGGT GAAAGAGTCA CTTACCAGTC 421 TAATAAGGCA AGAGGTTATA GCAAGGGCTG TCAACATTCA GAGCTCTGTG CAAACCGGAA 481 TCCCAGTCTT GTTGAACAAA AACAGCAGGG ATGTCATCCA GATGATTGAT AAGTCGTGCA 541 GCAGACAAGA GCTCACTCAG CACTGTGAGA GTACGATCGC AGTCCACCAT GCCGATGGAA 601 TTGCCCCACT TGAGCCACAT AGTTTCTGGA GATGCCCTGT CGGAGAACCG TATCTTAGCT 661 CAGATCCTGA AATCTCATTG CTGCCTGGTC CGAGCTTGTT ATCTGGTTCT ACAACGATCT 721 CTGGATGTGT TAGGCTCCCT TCACTCTCAA TTGGCGAGGC AATCTATGCC TATTCATCAA 781 ATCTCATTAC ACAAGGTTGT GCTGACATAG GGAAATCATA TCAGGTCCTG CAGCTAGGGT 841 ACATATCACT CAATTCAGAT ATGTTCCCTG ATCTTAACCC CGTAGTGTCC CACACTTATG 901 ACATCAACGA CAATCGGAAA TCATGCTCTG TGGTGGCAAC CGGGACTAGG GGTTATCAGC 961 TTTGCTCCAT GCCGACTGTA GACGAAAGAA CCCGACTACT CTAGTGATGG TATTTGAGGA 1021 TCTGGTCCCT TGATGTCCTG GATCTCAAAG GGAGACTAAG TCTCACCGGG TATCGCAACA 1081 GCGAGTAGAT CTGATCACCC GATTCTCTGG CACTATACCC CAGTGTAGCA ACGCATGGCC 1141 AACAGAAAGG GCTCATGAAT TTCTTGCGTA TTGTGCTTGC AC //
Primer: revserse2 () Sequence file: RDB13249_A5D3b.seq
>01542_13249_A5D3_3_revserse2_F02_17.ab1 1 AAGGAAATGA TTACGCCAGC GCGCAATTAA CCCTCACTAA AGGGAACAAA AGCTGGAGCT 61 CCACCGCGGT GGCGGCCGCT CTAGAACTAG TGGATCCCAG CTTCCCATTC ACCCTGGGTT 121 TTTCTTAATA CTGTGAGAGA CGTAAGAGAC TGAGAGATAT TGTGGATGTT CGGAGATGAC 181 TCTAGTGTCA GCAAGGCCGA CAAGCCTGCT AGTCAGTTAA ATTTAAGACT CGGCCTTGCA 241 TAATTTAGGG ATGCTAGTCT TAAAGAGCAT CGGCTGTAAG GTATTCAGGC TCTTCTGATT 301 GATCTCGATG ATGTGAAAGC AGTAGCCTTT ACCAAAATGC GTGATACACG ATGTCGTGGT 361 ATATGCAGCC TCTAATTGAA CATCCTTTAT CCTTAACATA TTTATAATGT TAGTAGTGTT 421 AGAATACATG ATTGTTGGGT TGACACGCGA TGTATTGGCA TATAGCGTGA CGGTAGCGAC 481 GTTAGCTGCA TCAGGGGACA ATGGATAAGC ATCAGTGTAT ACGCCTGATA TGCATTCCTT 541 CGGACACTTA TTGTACCAAT TGCACTCTTT ATTTCCTGGT CTAGACAAGG CTTCATGAGG 601 TGTCCAGTTG ATAGTCAAAG GGTGGCTGAC ATCAAGTACT CCTATCTGCA GTTGAGAGTG 661 CCAGCCTGAT GATCTTGTAT AGATGTACAC CCGATCACCC AATTTTAATA ATCTACCTTC 721 CGCCCCGAGA TAGTTTTGAG TGATTGGAAT GGTTGTGACT CTTATCTTTG GCCTCTCTGA 781 GAGATAGTCA TTGACCTGGA TGATCACGCT GACCACCTGT TTCCCTCCTA GCCATGTAAT 841 TTTCAGAGCC TCATTGCATG TGTCTTGCGA CACCTGTTGG CATCCTTGGG TCCTACATTT 901 TGTATCACCC TGCAGAGGGG TGGTTAGTCC ACCATACCCA AGAAATATCA ATGAGCCTTC 961 TGTTGCAATG CCGTTGCCTA CACTGGGGTA TAGTGCAGAG AACGGGTGAT CAAGATCTAC 1021 CCTCGCTGTT GCGATACCGG GTGAGACTTT AGTTCTCCCT TTGAGATCCA GGGACATCAA 1081 GGACCAGATC TCATACCATC ACTAGAGTAG TCCGCTCCTT CGTCTACAGT CGGCAATGAG 1141 CCAAGGCTGG ATTACTCCTA GTCGGTTGAC ACTCACAGAG CTAGTGAAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Taira, H., Construction of expression plasmids for the fusion protein of Sendai virus, and their expression in E. coli cells and eucaryotic cells. FEBS Lett., 262, 39-41 (1990). PMID 2156733.
original	Shioda, T., Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequences of the F, HN and L proteins. Nucleic Acids Res., 14, 1545-1563 (1986). PMID 3005975. [link to RRC of NBRP]