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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBS-HN

Sendai virus HN protein

Catalog number RDB01542
Resource name pBS-HN
Alternative name Sendai virus HN protein
Clone info. Sendai virus HN protein
Vector backbone pBluescript SK (+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Sendai virus Sendai virus HN glycoprotein cDNA
Depositor Taira, Hideharu | Shioda, Tatsuo |

Distribution information

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Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for not-for-profit research in the not-for-profit organization.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01542 pBS-HN DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Map RDB01542.gif

Original reference

original Taira, H., Construction of expression plasmids for the fusion protein of Sendai virus, and their expression in E. coli cells and eucaryotic cells. FEBS Lett., 262, 39-41 (1990). PMID 2156733.
original Shioda, T., Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequences of the F, HN and L proteins. Nucleic Acids Res., 14, 1545-1563 (1986). PMID 3005975.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13249_A5D3p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13(-40)
Sequence file: RDB13249_A5D3a.seq check
>01542_13249_A5D3_3_M13(-40)_E02_14.ab1
    1 AGTCAAGGGA GCGCGCGTAT ACGACTCACT ATAGGGCGAA TTGGGTACCG GGCCCCCCCT
   61 CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCCTG CAGCCCGGGG GATCCCCGGT
  121 ACTTTAGCTT TCACCTCAAA CAAGCACAGA TCATGGATGG TGATAGGGGC AAACGTGACT
  181 CGTACTGGTC TACTTCTCCT AGTGGTAGCA CCACAAAACC AGCATCAGGT TGGGAGAGGT
  241 CAAGTAAAGC CGACACATGG TTGCTGATTC TCTCATTCAC CCAGTGGGCT TTGTCAATTG
  301 CCACAGTGAT CATCTGTATC ATAATTTCTG CTAGACAAGG GTATAGTATG AAAGAGTACT
  361 CAATGACTGT AGAGGCATTG AACATGAGCA GCAGGGAGGT GAAAGAGTCA CTTACCAGTC
  421 TAATAAGGCA AGAGGTTATA GCAAGGGCTG TCAACATTCA GAGCTCTGTG CAAACCGGAA
  481 TCCCAGTCTT GTTGAACAAA AACAGCAGGG ATGTCATCCA GATGATTGAT AAGTCGTGCA
  541 GCAGACAAGA GCTCACTCAG CACTGTGAGA GTACGATCGC AGTCCACCAT GCCGATGGAA
  601 TTGCCCCACT TGAGCCACAT AGTTTCTGGA GATGCCCTGT CGGAGAACCG TATCTTAGCT
  661 CAGATCCTGA AATCTCATTG CTGCCTGGTC CGAGCTTGTT ATCTGGTTCT ACAACGATCT
  721 CTGGATGTGT TAGGCTCCCT TCACTCTCAA TTGGCGAGGC AATCTATGCC TATTCATCAA
  781 ATCTCATTAC ACAAGGTTGT GCTGACATAG GGAAATCATA TCAGGTCCTG CAGCTAGGGT
  841 ACATATCACT CAATTCAGAT ATGTTCCCTG ATCTTAACCC CGTAGTGTCC CACACTTATG
  901 ACATCAACGA CAATCGGAAA TCATGCTCTG TGGTGGCAAC CGGGACTAGG GGTTATCAGC
  961 TTTGCTCCAT GCCGACTGTA GACGAAAGAA CCCGACTACT CTAGTGATGG TATTTGAGGA
 1021 TCTGGTCCCT TGATGTCCTG GATCTCAAAG GGAGACTAAG TCTCACCGGG TATCGCAACA
 1081 GCGAGTAGAT CTGATCACCC GATTCTCTGG CACTATACCC CAGTGTAGCA ACGCATGGCC
1141 AACAGAAAGG GCTCATGAAT TTCTTGCGTA TTGTGCTTGC AC
//
Primer: revserse2
Sequence file: RDB13249_A5D3b.seq check
>01542_13249_A5D3_3_revserse2_F02_17.ab1
    1 AAGGAAATGA TTACGCCAGC GCGCAATTAA CCCTCACTAA AGGGAACAAA AGCTGGAGCT
   61 CCACCGCGGT GGCGGCCGCT CTAGAACTAG TGGATCCCAG CTTCCCATTC ACCCTGGGTT
  121 TTTCTTAATA CTGTGAGAGA CGTAAGAGAC TGAGAGATAT TGTGGATGTT CGGAGATGAC
  181 TCTAGTGTCA GCAAGGCCGA CAAGCCTGCT AGTCAGTTAA ATTTAAGACT CGGCCTTGCA
  241 TAATTTAGGG ATGCTAGTCT TAAAGAGCAT CGGCTGTAAG GTATTCAGGC TCTTCTGATT
  301 GATCTCGATG ATGTGAAAGC AGTAGCCTTT ACCAAAATGC GTGATACACG ATGTCGTGGT
  361 ATATGCAGCC TCTAATTGAA CATCCTTTAT CCTTAACATA TTTATAATGT TAGTAGTGTT
  421 AGAATACATG ATTGTTGGGT TGACACGCGA TGTATTGGCA TATAGCGTGA CGGTAGCGAC
  481 GTTAGCTGCA TCAGGGGACA ATGGATAAGC ATCAGTGTAT ACGCCTGATA TGCATTCCTT
  541 CGGACACTTA TTGTACCAAT TGCACTCTTT ATTTCCTGGT CTAGACAAGG CTTCATGAGG
  601 TGTCCAGTTG ATAGTCAAAG GGTGGCTGAC ATCAAGTACT CCTATCTGCA GTTGAGAGTG
  661 CCAGCCTGAT GATCTTGTAT AGATGTACAC CCGATCACCC AATTTTAATA ATCTACCTTC
  721 CGCCCCGAGA TAGTTTTGAG TGATTGGAAT GGTTGTGACT CTTATCTTTG GCCTCTCTGA
  781 GAGATAGTCA TTGACCTGGA TGATCACGCT GACCACCTGT TTCCCTCCTA GCCATGTAAT
  841 TTTCAGAGCC TCATTGCATG TGTCTTGCGA CACCTGTTGG CATCCTTGGG TCCTACATTT
  901 TGTATCACCC TGCAGAGGGG TGGTTAGTCC ACCATACCCA AGAAATATCA ATGAGCCTTC
  961 TGTTGCAATG CCGTTGCCTA CACTGGGGTA TAGTGCAGAG AACGGGTGAT CAAGATCTAC
 1021 CCTCGCTGTT GCGATACCGG GTGAGACTTT AGTTCTCCCT TTGAGATCCA GGGACATCAA
 1081 GGACCAGATC TCATACCATC ACTAGAGTAG TCCGCTCCTT CGTCTACAGT CGGCAATGAG
1141 CCAAGGCTGG ATTACTCCTA GTCGGTTGAC ACTCACAGAG CTAGTGAAT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Taira, H., Construction of expression plasmids for the fusion protein of Sendai virus, and their expression in E. coli cells and eucaryotic cells. FEBS Lett., 262, 39-41 (1990). PMID 2156733.
original Shioda, T., Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequences of the F, HN and L proteins. Nucleic Acids Res., 14, 1545-1563 (1986). PMID 3005975.

2020.01.06

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