RDB01530 - pCAmB7

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pCAmB7 (#RDB01530)

Expression vector of mouse B7 (CD80) cDNA

Alternative name	Mouse B7 (CD80)
Clone info.	Expression vector of mouse B7 (CD80) gene in mammalian cells
Vector backbone	pCAGGS (Plasmid)
Size of vector backbone	4.8 kb
Selectable markers	Amp^r (E. coli)
Gene/insert name	Mouse CD80 (B7-1) cDNA
Depositor\|Developer	Hamada, Hirofumi \|

Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Cell, 33, 135-143 (1983); J. Virol., 45, 555-564 (1983).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Cell, 33, 135-143 (1983); J. Virol., 45, 555-564 (1983).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Cell, 33, 135-143 (1983); J. Virol., 45, 555-564 (1983).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Cell, 33, 135-143 (1983); J. Virol., 45, 555-564 (1983).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01530	pCAmB7	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAmB7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01530).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18580_B0J1p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CAG_R1 (Pr0584) Region: CAG pro,insert 5' Sequence file: RDB18580_B0J1a.seq
>01530_18580_B0J1_1_CAG_R1_2.5_A12_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NTCNNNCGGG CGCGGGGCGA ANCGGTGCGG CGCCGGCAGG 61 AAGGAAATGG GCGGGGAGGG CCTTCGTGCG TCGCCGCGCC GCCGTCCCCT TCTCCCTCTC 121 CAGCCTCGGG GCTGTCCGCG GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG 181 TTCGGCTTCT GGCGTGTGAC CGGCGGCTCT AGAGCCTCTG CTAACCATGT TCATGCCTTC 241 TTCTTTTTCC TACAGCTCCT GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC 301 AAAGAATTAG ACGGCATCGC AGCTTGGATA CACGCCGCCC ACGTGAAGGC TGCCGACCCC 361 GGGGGTGGAC CATCCTCTAG ACTGCCATGG CTTGCAATTG TCAGTTGATG CAGGATACAC 421 CACTCCTCAA GTTTCCATGT CCAAGGCTCA TTCTTCTCTT TGTGCTGCTG ATTCGTCTTT 481 CACAAGTGTC TTCAGATGTT GATGAACAAC TGTCCAAGTC AGTGAAAGAT AAGGTATTGC 541 TGCCTTGCCG TTACAACTCT CCTCATGAAN ANGAGTCTGA AGANCGAATC TACTGNCAAA 601 AACATGACAA AGTGGTGCTG TCTGTCATTG CTGGGAAACT AAAAGTGTGG NCCGANTATN 661 AGAACCGGAN TTTATATGAN NACACTACCT ACTCTCTTAT CNTCNTNGGN CNNNNNNNTT 721 NNNANNNNGG NNNNNNNNNN //
Primer: pCAGGS_R1 (Pr0614) Region: insert 3' Sequence file: RDB18580_B0J1b.seq
>01530_18580_B0J1_1_pCAGGS_R1_B02_02_ABI08.ab1 1 NNNNNNNNNN NNNNNCNATT GNATGNAAAN AAATTTCCTT TATTAGCCNG AAGTCAGATG 61 CTCAAGGGGC TTCATGATGT CCCCATAATT TTTGGCAGAG GGAAAAAGAT CTCAGTGGTA 121 TTTGTGAGCC AGGGCATTGG CCACACCAGC CACCACCTTC TGATAGGCAG CCTGCACCTG 181 AGGAGTGAAT TATCCTGTCT TTAACAAATT GGACTAATCC GGATCTAAAG GAAGACGGTC 241 TGTTCAGCTA ATGCTTCTTC AGGCCCGAAG GTAAGGCTGT TGTTTGTTTC TCTGCTTGCC 301 TCATTTCTTC TGAAACAGCT TCTGTGCTTA CAGAAGCATT TGATGATGAC AACGATGACG 361 ACGACTGTTA TTACTGCGCC GAATCCTGCC CCAAAGAGCA CAAGTGTGTT CTTGCTATCA 421 GGAGGGTCTT CTGGGGGTTT TTCCCAGGTG AAGTCCTCTG ACACGTGAGC ATCTCCATAT 481 TTAATGAGAC ACTTAATGGT GTGGTTGCGA GTCGTATTGA AATCTAGTTG GCTACTAATG 541 GTGTACAATT CAGATTCAGG ATCCTGGGAA ATTGTCGTAT TGATGCCAGG TAATTCTCTT 601 CCATTTTCCA ACCAAGAGAA GCGAGGCTTT GGGAAACCCC CGGAAGCAAA GCAGGTAATC 661 CTTTTAGTGT CTGCAGATGG GTTTCCAGAC TCAGTTATGT TGGGGGTAGA GAAGTCAGCT 721 TTGATGGACA ACTTTACTAA AGCCAAGTGT TTAACTTCAT ACGTTCCTCT TTCCTTCTTT 781 TGAACGACAC AGCTGTATGT GCCCCGGTCT GAAAGGACCA GGCCCAGGNT GANAAGAGAG 841 TANGTAGTGT TGTCATATAA AGTCCGGTTC TTATACTCNG GCCACACTTT TANTTNCCCA 901 GCAATGACAG ACAGCACCAC TTNNNCATGT TTTTGCNNGT ANANTCNGNC TTCNNNNTCA 961 TCTTNNTNNN GNNNNNNGNN NNNGNNNNGN NNNNNNNNN //