RDB01526 - pCAhMIF

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pCAhMIF (#RDB01526)

Expression vector of human macrophage migration inhibitory factor

Alternative name	Human macrophage migration inhibitory factor
Clone info.	Expression vector of human macrophage migration inhibitory factor gene in mammalian cells.
Vector backbone	pCAGGS (Plasmid)
Size of vector backbone	4.8 kb
Selectable markers	Amp^r (E. coli)
Gene/insert name	Human MIF cDNA
Depositor\|Developer	Hamada, Hirofumi \|

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Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR and an acknowledgment to the DEPOSITOR are requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用し、研究成果の公表にあたって謝辞の表明を必要とする。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用し、研究成果の公表にあたって謝辞の表明を必要とする。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01526	pCAhMIF	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAhMIF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01526).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15998_A8Gop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F Sequence file: RDB15998_A8Goa.seq
>01526_15998_A8Go_1_pCAG-F_A01_01_ABI24.ab1 1 GGGGGTTTTC CGGGGCTTTC TGGCGTGTGA CCGGCGGCTC TACTATCCTC TGCTAACCAT 61 GTTCATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG TGCTGGTTAT TGTGCTGTCT 121 CATCATTTTG GCAAAGAATT CTAGACCATC ATGCCGATGT TCATCGTAAA CACCAACGTG 181 CCCCGCGCCT CCGTGCCGGA CGGGTTCCTC TCCGAGCTCA CCCAGCAGCT GGCGCAGGCC 241 ACCGGCAAGC CCCCCCAGTA CATCGCGGTG CACGTGGTCC CGGACCAGCT CATGGCCTTC 301 GGCGGCTCCA GCGAGCCGTG CGCGCTCTGC AGCCTGCACA GCATCGGCAA GATCGGCGGC 361 GCGCAGAACC GCTCCTACAG CAAGCTGCTG TGCGGCCTGC TGGCCGAGCG CCTGCGCATC 421 AGCCCGGACA GGGTCTACAT CAACTATTAC GACATGAACG CGGCCAATGT GGGCTGGAAC 481 AACTCCACCT TCGCCTAAGG ATCTTTTTCC CTCTGCCAAA AATTATGGGG ACATCATGAA 541 GCCCCTTGAG CATCTGACTT CTGGCTAATA AAGGAAATTT ATTTTCATTG CAATAGTGTG 601 TTGGAATTTT TTGTGTCTCT CACTCGGAAG GACATATGGG AGGGCAAATC ATTTAAAACA 661 TCAGAATGAG TATTTGGTTT AGAGTTTGGC AACATATGCC CATATGCTGG CTGCCATGAA 721 CAAAGGTTTG GCTATAAAGA GGTCATCAGT ATATGAAACA GCCCCCTGCT GTCCATTCCG 781 TTATTCCATA GAAAAGCCTT GACTTGAGGT TTAGATTTTT TTTTTATATT TTGTTTTGTG 841 TTATTTTTGT TTCGTTTTAA CATCCCCTAA AAAATTTTTC CCTTTACATG GTTTTTTACT 901 AAGCCAGAAA TTTTTTCCAT CCCTGCTCCC TGAACTTACG TCCT //
Primer: pCA-bgter Sequence file: RDB15998_A8Gob.seq
>01526_15998_A8Go_1_pCA-bgter_B01_04_ABI24.ab1 1 AGCCCGGAAG TCCATAATTT TTGGCAGAGG GAAAAAGATC CTTAGGCGAA GGTGGAGTTG 61 TTCCAGCCCA CATTGGCCGC GTTCATGTCG TAATAGTTGA TGTAGACCCT GTCCGGGCTG 121 ATGCGCAGGC GCTCGGCCAG CAGGCCGCAC AGCAGCTTGC TGTAGGAGCG GTTCTGCGCG 181 CCGCCGATCT TGCCGATGCT GTGCAGGCTG CAGAGCGCGC ACGGCTCGCT GGAGCCGCCG 241 AAGGCCATGA GCTGGTCCGG GACCACGTGC ACCGCGATGT ACTGGGGGGG CTTGCCGGTG 301 GCCTGCGCCA GCTGCTGGGT GAGCTCGGAG AGGAACCCGT CCGGCACGGA GGCGCGGGGC 361 ACGTTGGTGT TTACGATGAA CATCGGCATG ATGGTCTAGA ATTCTTTGCC AAAATGATGA 421 GACAGCACAA TAACCAGCAC GTTGCCCAGG AGCTGTAGGA AAAAGAAGAA GGCATGAACA 481 TGGTTAGCAG AGGCTCTAGA GCCGCCGGTC ACACGCCAGA AGCCGAACCC CGCCCTGCCC 541 CGTCCCCCCC GAAGGCAGCC GTCCCCCCGC GGACAGCCCC GAGGCTGGAG AGGGAGAAGG 601 GGACGGCGGC GCGGCGACGC ACGAAGGCCC TCCCCGCCCA TTTCCTTCCT GCCGGCGCCG 661 CACCGCTTCG CCCCGCGCCC GCTAGAGGGG GTGCGGCGGC GCCTCCCAGA TTTCGGCTCC 721 GCACAGATTT GGGACAAAGG AAGTCCCTGC GCCCTCTCGC ACGATTACCA TAAAAGGCAA 781 TGGCTGCGGC TCGCCGCGCC TCGACAGCCG CCGGCGCTCC GGGGGGCCGC CGCGCCCCCT 841 CCCCCGAGCC CTCCCCGGCC CGAGGCGGCC CCGCCCCCGC CCGGGCACCC CCCACCCTGC 901 CGCCACCCCC CCGCCCGGGC ACGGCGAGCC CCGCGCCCAC GCCCCCGTAC GGGAGCCCCG 961 CACCCGAAGC CCGGGCCCGT GCTCAGCAAC TCTGGAGGGG GGTGCAGCAG TGGGGGTGCC 1021 GGGCGGAGCG GGGCCGCCTT CGGGCCGAGA GGCTCGGGGG AGGGGCGTCG G //