Resource data sheet
Prev.

pCAhMIF

Expression vector of human macrophage migration inhibitory factor

Catalog number RDB01526
Resource name pCAhMIF
Alternative name Human macrophage migration inhibitory factor
Clone info. Expression vector of human macrophage migration inhibitory factor gene in mammalian cells.
.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r (E. coli)
Gene/insert name Human MIF cDNA
Depositor Hamada, Hirofumi |
 
Other clones in our bank human MIF (NCBI Gene 4282) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR and an acknowledgment to the DEPOSITOR are requested.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01526 pCAhMIF DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01526.gif from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15998_A8Gop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F RDB15998_A8Goa.seq
>01526_15998_A8Go_1_pCAG-F_A01_01_ABI24.ab1
    1 GGGGGTTTTC CGGGGCTTTC TGGCGTGTGA CCGGCGGCTC TACTATCCTC TGCTAACCAT
   61 GTTCATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG TGCTGGTTAT TGTGCTGTCT
  121 CATCATTTTG GCAAAGAATT CTAGACCATC ATGCCGATGT TCATCGTAAA CACCAACGTG
  181 CCCCGCGCCT CCGTGCCGGA CGGGTTCCTC TCCGAGCTCA CCCAGCAGCT GGCGCAGGCC
  241 ACCGGCAAGC CCCCCCAGTA CATCGCGGTG CACGTGGTCC CGGACCAGCT CATGGCCTTC
  301 GGCGGCTCCA GCGAGCCGTG CGCGCTCTGC AGCCTGCACA GCATCGGCAA GATCGGCGGC
  361 GCGCAGAACC GCTCCTACAG CAAGCTGCTG TGCGGCCTGC TGGCCGAGCG CCTGCGCATC
  421 AGCCCGGACA GGGTCTACAT CAACTATTAC GACATGAACG CGGCCAATGT GGGCTGGAAC
  481 AACTCCACCT TCGCCTAAGG ATCTTTTTCC CTCTGCCAAA AATTATGGGG ACATCATGAA
  541 GCCCCTTGAG CATCTGACTT CTGGCTAATA AAGGAAATTT ATTTTCATTG CAATAGTGTG
  601 TTGGAATTTT TTGTGTCTCT CACTCGGAAG GACATATGGG AGGGCAAATC ATTTAAAACA
  661 TCAGAATGAG TATTTGGTTT AGAGTTTGGC AACATATGCC CATATGCTGG CTGCCATGAA
  721 CAAAGGTTTG GCTATAAAGA GGTCATCAGT ATATGAAACA GCCCCCTGCT GTCCATTCCG
  781 TTATTCCATA GAAAAGCCTT GACTTGAGGT TTAGATTTTT TTTTTATATT TTGTTTTGTG
  841 TTATTTTTGT TTCGTTTTAA CATCCCCTAA AAAATTTTTC CCTTTACATG GTTTTTTACT
901 AAGCCAGAAA TTTTTTCCAT CCCTGCTCCC TGAACTTACG TCCT
//
Primer: pCA-bgter RDB15998_A8Gob.seq
>01526_15998_A8Go_1_pCA-bgter_B01_04_ABI24.ab1
    1 AGCCCGGAAG TCCATAATTT TTGGCAGAGG GAAAAAGATC CTTAGGCGAA GGTGGAGTTG
   61 TTCCAGCCCA CATTGGCCGC GTTCATGTCG TAATAGTTGA TGTAGACCCT GTCCGGGCTG
  121 ATGCGCAGGC GCTCGGCCAG CAGGCCGCAC AGCAGCTTGC TGTAGGAGCG GTTCTGCGCG
  181 CCGCCGATCT TGCCGATGCT GTGCAGGCTG CAGAGCGCGC ACGGCTCGCT GGAGCCGCCG
  241 AAGGCCATGA GCTGGTCCGG GACCACGTGC ACCGCGATGT ACTGGGGGGG CTTGCCGGTG
  301 GCCTGCGCCA GCTGCTGGGT GAGCTCGGAG AGGAACCCGT CCGGCACGGA GGCGCGGGGC
  361 ACGTTGGTGT TTACGATGAA CATCGGCATG ATGGTCTAGA ATTCTTTGCC AAAATGATGA
  421 GACAGCACAA TAACCAGCAC GTTGCCCAGG AGCTGTAGGA AAAAGAAGAA GGCATGAACA
  481 TGGTTAGCAG AGGCTCTAGA GCCGCCGGTC ACACGCCAGA AGCCGAACCC CGCCCTGCCC
  541 CGTCCCCCCC GAAGGCAGCC GTCCCCCCGC GGACAGCCCC GAGGCTGGAG AGGGAGAAGG
  601 GGACGGCGGC GCGGCGACGC ACGAAGGCCC TCCCCGCCCA TTTCCTTCCT GCCGGCGCCG
  661 CACCGCTTCG CCCCGCGCCC GCTAGAGGGG GTGCGGCGGC GCCTCCCAGA TTTCGGCTCC
  721 GCACAGATTT GGGACAAAGG AAGTCCCTGC GCCCTCTCGC ACGATTACCA TAAAAGGCAA
  781 TGGCTGCGGC TCGCCGCGCC TCGACAGCCG CCGGCGCTCC GGGGGGCCGC CGCGCCCCCT
  841 CCCCCGAGCC CTCCCCGGCC CGAGGCGGCC CCGCCCCCGC CCGGGCACCC CCCACCCTGC
  901 CGCCACCCCC CCGCCCGGGC ACGGCGAGCC CCGCGCCCAC GCCCCCGTAC GGGAGCCCCG
  961 CACCCGAAGC CCGGGCCCGT GCTCAGCAAC TCTGGAGGGG GGTGCAGCAG TGGGGGTGCC
1021 GGGCGGAGCG GGGCCGCCTT CGGGCCGAGA GGCTCGGGGG AGGGGCGTCG G
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

GNP_filter3_RDBDEP_html_181012.pl