RDB01518 - pCAmIL1RA

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pCAmIL1RA (#RDB01518)

Expression vector of mouse IL-1RA (Receptor antagonist), cDNA

Alternative name	Mouse IL-1 RA (receptor antagonist)
Clone info.	Expression vector of mouse IL-1 RA (receptor antagonist) gene in mammalian cells. Please note that IL1RA variant 2 is cloned.
Vector backbone	pCAGGS (Plasmid)
Size of vector backbone	4.8 kb
Selectable markers	Amp^r (E. coli)
Gene/insert name	Mouse IL-1 receptor antagonist cDNA
Depositor\|Developer	Hamada, Hirofumi \|

Additional map
Remarks, protocol and/or map (pdf)	RDB01518.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Cell, 33, 135-143 (1983); J. Virol., 45, 555-564 (1983).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Cell, 33, 135-143 (1983); J. Virol., 45, 555-564 (1983).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Cell, 33, 135-143 (1983); J. Virol., 45, 555-564 (1983).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Cell, 33, 135-143 (1983); J. Virol., 45, 555-564 (1983).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01518	pCAmIL1RA	DNA solution	Please contact us.

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAmIL1RA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01518).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13437_A5Fg.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB13437_A5Fga.seq
>01518_13437_A5Fg_pAxCA_F1_C01_07.ab1 1 CTACCTGGCT ACATGTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC AACGTGCTGG 61 TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATAGACGGC ATCGCAGCTT GGATACACGC 121 CGCCCACGTG AAGGCTGCCG ACCCCGGGGG TGGACCATCC TCTAGACTGC CATGGAAATC 181 TGCTGGGGAC CCTACAGTCA CCTAATCTCT CTCCTTCTCA TCCTTCTGTT TCATTCAGAG 241 GCAGCCTGCC GCCCTTCTGG GAAAAGACCC TGCAAGATGC AAGCCTTCAG AATCTGGGAT 301 ACTAACCAGA AGACCTTTTA CCTGAGAAAC AACCAGCTCA TTGCTGGGTA CTTACAAGGA 361 CCAAATATCA AACTAGAAGA AAAGATAGAC ATGGTGCCTA TTGACCTTCA TAGTGTGTTC 421 TTGGGCATCC ACGGGGGCAA GCTGTGCCTG TCTTGTGCCA AGTCTGGAGA TGATATCAAG 481 CTCCAGCTGG AGGAAGTTAA CATCACTGAT CTGAGCAAGA ACAAAGAAGA AGACAAGCGC 541 TTTACCTTCA TCCGCTCTGA GAAAGGCCCC ACCACCAGCT TTGAGTCAGC TGCCTGTCCA 601 GGATGGTTCC TCTGCACAAC ACTAGAGGCT GACCGTCCTG TGAGCCTCAC CAACACACCG 661 GAAGAGCCCC TTATAGTCAC GAAGTTCTAC TTCCAGGAAG ACCAATAGTA CTGCCGAGGC 721 GGATCCGGAT TAGTCCAATT TGTTAAAGAC AGGATATCAG TGGTCCAGGC TCTAGTTTTG 781 ACTCAACAAT ATCACCAGCT GAAGCCTATA GAGTACGAGC CATAGATAAA ATAAAAGATT 841 TTATTTAGTC TCCAGAAAAA GGGGGGAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA 901 GAATTCACTC CTCAGGTGCA GGCTGCCTAT CAGAATGTGG TGGCTGGTGT GGCCAATGCC 961 CTGTCTCACA AATACCACTG AGATCTTTTT CCCTCTGCCA AAAATTATGG GGACATCATG 1021 AAGCCCCTTG AGCATCTGAC TTCTGGCTAA TAAAAGAAAT TTATTTCATG CATAGTGTGT 1081 TGGGATTTTT GTGTCTCTCA CTCGAGAATA TGGGAGCATC ATCAAACATC AGAATGGAGT 1141 ATTTTGGGTT AGAGAGTTGG CCACC //

Primer: pAxCA_F1
Sequence file: RDB13437_A5Fga.seq check

>01518_13437_A5Fg_pAxCA_F1_C01_07.ab1
    1 CTACCTGGCT ACATGTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC AACGTGCTGG
   61 TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATAGACGGC ATCGCAGCTT GGATACACGC
  121 CGCCCACGTG AAGGCTGCCG ACCCCGGGGG TGGACCATCC TCTAGACTGC CATGGAAATC
  181 TGCTGGGGAC CCTACAGTCA CCTAATCTCT CTCCTTCTCA TCCTTCTGTT TCATTCAGAG
  241 GCAGCCTGCC GCCCTTCTGG GAAAAGACCC TGCAAGATGC AAGCCTTCAG AATCTGGGAT
  301 ACTAACCAGA AGACCTTTTA CCTGAGAAAC AACCAGCTCA TTGCTGGGTA CTTACAAGGA
  361 CCAAATATCA AACTAGAAGA AAAGATAGAC ATGGTGCCTA TTGACCTTCA TAGTGTGTTC
  421 TTGGGCATCC ACGGGGGCAA GCTGTGCCTG TCTTGTGCCA AGTCTGGAGA TGATATCAAG
  481 CTCCAGCTGG AGGAAGTTAA CATCACTGAT CTGAGCAAGA ACAAAGAAGA AGACAAGCGC
  541 TTTACCTTCA TCCGCTCTGA GAAAGGCCCC ACCACCAGCT TTGAGTCAGC TGCCTGTCCA
  601 GGATGGTTCC TCTGCACAAC ACTAGAGGCT GACCGTCCTG TGAGCCTCAC CAACACACCG
  661 GAAGAGCCCC TTATAGTCAC GAAGTTCTAC TTCCAGGAAG ACCAATAGTA CTGCCGAGGC
  721 GGATCCGGAT TAGTCCAATT TGTTAAAGAC AGGATATCAG TGGTCCAGGC TCTAGTTTTG
  781 ACTCAACAAT ATCACCAGCT GAAGCCTATA GAGTACGAGC CATAGATAAA ATAAAAGATT
  841 TTATTTAGTC TCCAGAAAAA GGGGGGAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA
  901 GAATTCACTC CTCAGGTGCA GGCTGCCTAT CAGAATGTGG TGGCTGGTGT GGCCAATGCC
  961 CTGTCTCACA AATACCACTG AGATCTTTTT CCCTCTGCCA AAAATTATGG GGACATCATG
1021 AAGCCCCTTG AGCATCTGAC TTCTGGCTAA TAAAAGAAAT TTATTTCATG CATAGTGTGT
1081 TGGGATTTTT GTGTCTCTCA CTCGAGAATA TGGGAGCATC ATCAAACATC AGAATGGAGT
1141 ATTTTGGGTT AGAGAGTTGG CCACC
//

Please visit Sequencing and PCR primers for primer information.