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pBluescript SKII+mLIF

Plasmid clone of mouse LIF (Leukemia inhibitory factor) cDNA

Catalog number RDB01484
Resource name pBluescript SKII+mLIF
Alternative name Mouse LIF (leukemia inhibitory factor) cDNA
Clone info. Mouse LIF (leukemia inhibitory factor) cDNA
Vector backbone pBluescript II SK (+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Mouse Leukemia inhibitory factor (LIF) cDNA
Depositor|Developer Hamada, Hirofumi |
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External Database
Mouse Leukemia inhibitory factor (LIF)
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB01484 pBluescript SKII+mLIF DNA solution Please contact us.

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB01484.gif

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19265_B1Itp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-M13_-100
          Region: T7 pro,insert 5'
          Sequence file: RDB19265_B1Ita.seq check
          >01484_19265_B1It_2_pUC-M13_-100_G01_07_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN ANGCCNGGGT TTTCCCNGTC NCGACGTTGT AAAACGACGG
             61 CCAGTGAGCG CGCGTAATAC GACTCACTAT AGGGCGAATT GGGTACCGGG CCCCCCCTCG
            121 AGGTCGACGG TATCGATAAG CTTGATATCG AATTCTAGAC ACCATGAAGG TCTTGGCCGC
            181 AGGGATTGTG CCCTTACTGC TGCTGGTTCT GCACTGGAAA CACGGGGCAG GGAGCCCTCT
            241 TCCCATCACC CCTGTAAATG CCACCTGTGC CATACGCCAC CCATGCCACG GCAACCTCAT
            301 GAACCAGATC AAGAATCAAC TGGCACAGCT CAATGGCAGC GCCAATGCTC TCTTCATTTC
            361 CTATTACACA GCTCAAGGGG AGCCGTTTCC CAACAACGTG GAAAAGCTAT GTGCGCCTAA
            421 CATGACAGAC TTCCCATCTT TCCATGGCAA CGGGACAGAG AAGACCAAGT TGGTGGAGCT
            481 GTATCGGATG GTCGCATACC TGAGCGCCTC CCTGACCAAT ATCACCCGGG ACCAGAAGGT
            541 CCTGAACCCC ACTGCCGTGA GCCTCCAGGT CAAGCTCAAT GCTACTATAG ACGTCATGAN
            601 GGGCCTCCTC AGCAATGTGC TTTGCCGTCT GTGCNACAAG TANCGTGTGG GCNACGNNNA
            661 NGTGCNANNN GTCCCNNANN ACTCNNNNNA ANANNCNNTN NNANNNNNAA NNNTNNGNNT
          721 NNNNNNNCNT NNNNGGGNNN NNNNNNN
          //
          Primer: reverse-2
          Region: T3 pro,insert 3'
          Sequence file: RDB19265_B1Itb.seq check
          >01484_19265_B1It_2_reverse2_H01_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CTCNNNNNNN NGGAACAAAA GCTGGAGCTC
             61 CACCGCGGTG GCGGCCGCTC TAGAACTAGT GGATCCTAGA AGGCCTGGAC CACCACACTT
            121 ATGACTTGCT TGTATGTCCC CAGAAGCTGG CAACCCAACT TTTTCCTTTG GAAGGCTTCT
            181 TTGTCAGAGT GGTCGGGGAC AGGTGGCACA TCCACGTGGC CCACACGGTA CTTGTTGCAC
            241 AGACGGCAAA GCACATTGCT GAGGAGGCCC CTCATGACGT CTATAGTAGC ATTGAGCTTG
            301 ACCTGGAGGC TCACGGCAGT GGGGTTCAGG ACCTTCTGGT CCCGGGTGAT ATTGGTCAGG
            361 GAGGCGCTCA GGTATGCGAC CATCCGATAC AGCTCCACCA ACTTGGTCTT CTCTGTCCCG
            421 TTGCCATGGA AAGATGGGAA GTCTGTCATG TTAGGCGCAC ATAGCTTTTC CACGTTGTTG
            481 GGAAACGGCT CCCCTTGAGC TGTGTAATAG GAAATGAAGA GAGCATTGGC GCTGCCATTG
            541 AGCTGTGCCA GTTGATTCTT GATCTGGTTC ATGAGGTTGC CGTGGCATGG GTGGCGTATG
            601 GCACAGGTGG CATTTACAGG GGTGNNNNNN NAGANGGCTC CCNGCCCCGT GTTTCCNGTG
            661 CANNNNNNGC AGCNNNNNNN GCNNNNTCCC TGCGGNNNNN ANNTTNATGN NGNCNANNNN
          721 TNNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          user report Nakamura H., Alteration of the timing of implantation by in vivo gene transfer: delay of implantation by suppression of nuclear factor kappaB activity and partial rescue by leukemia inhibitory factor. Biochem. Biophys. Res. Commun., 321, 886-892 (2004). PMID 15358109.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl