RDB01480 - pBluescript KS+mLT

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pBluescript KS+mLT (#RDB01480)

Mouse lymphotoxin (TNF-beta) cDNA

Alternative name	Mouse lymphotoxin (TNF-beta) cDNA
Clone info.	Mouse lymphotoxin (TNF-beta) cDNA. T3 promoter region on vector sequence may be deleted.
Vector backbone	pBluescript KS (+) (Plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Amp^r
Gene/insert name	Mouse lymphotoxin A (LT, TNF-beta) cDNA
Depositor\|Developer	Hamada, Hirofumi \|

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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01480	pBluescript KS+mLT	DNA solution	Please contact us.

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBluescript KS+mLT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01480).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14248_A6Gdp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB14248_A6Gda.seq
>01480_14248_A6Gd_M13(-40)_A01_01.ab1 1 AAACGGGTAA TTGTAATACG ACTCACTATA GGGCGAATTG GAGCTCCACC GCGGTGGCGG 61 CCGCTCTAGA ACTAGTGGAT CCTACAGTGC AAAGGCTCCA AAGAATACAC TGCTGGGGCT 121 GAAGTGTAGA TGGGAGATGC CGTCGGTGTG GGTGGACAGC TGGTCTCCCT TACTGAGCAG 181 GAACACAGCC CCCTGGTACA TTGAGCGCAC CCACGGTCCT TGAAGTCCCG GATACACAGA 241 CTTCTGCGCA CTGAGGAGAG GCACATGGAA GGGGTATTGG GAGGAAAAGA GCTGGACCTC 301 GTGTGCCAGG TAGATGGGAG TGGGAATGGC CCTGGGGGAG CAGCTTTCTC CAGAGAAAAC 361 CACCTGGGAG TAGACAAAGT AGAGGCCACT GGTGGGGATC AGGAGGGAGT TGTTGCTCAA 421 AGAGAAGCCA TGTCGGAGAA AGGCACGATC CGTGCTTGCT CTCCAGAGCA GTGAGTTCTG 481 CTTGCTGGGG TACCCAACAA GGTGAGCAGC AGGTTTCAGG ATGCCATGGG TCAAGTGCTT 541 CTGAGGGAGT GGATGGGCTG TCCTGGCAGC GGAGAAGCGG ACACCAGAGA GTCCCTGGGC 601 CCCTAGAGGC AGGGCCAGCA GCAGCCCCAG GAGGAAGACA GGAGGGGTGC CAAGCACCCT 661 CAAGAGGTGG AGACGGCCGA GCAGTGTCAT GGTGTCTAGA ATTCGCTTTC CAGTCGGGAA 721 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 781 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 841 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA CGGGATAACG 901 CAGGAAAGAA CATGTGAGCA AAAAGGCCAG CCAAAAGGCC AGGGAACCCG TAAAAAGGCC 961 GCGTTTGCCT GGCCGTTTTT TCCATAGGCC TCCGCCCCCC CCTGACGAGC CATCACCAAA 1021 AATCGACGCC TCCAAAGTCA AGAGGTGGCC GGAAACCCCG ACCAGGACCT ATAGGATACC 1081 AGCCGCTTTC CCCCTTGGAA GGCCTCTTCG ATGACGACCT TCCATCCCCA TG //

Primer: M13_-40
Sequence file: RDB14248_A6Gda.seq check

>01480_14248_A6Gd_M13(-40)_A01_01.ab1
    1 AAACGGGTAA TTGTAATACG ACTCACTATA GGGCGAATTG GAGCTCCACC GCGGTGGCGG
   61 CCGCTCTAGA ACTAGTGGAT CCTACAGTGC AAAGGCTCCA AAGAATACAC TGCTGGGGCT
  121 GAAGTGTAGA TGGGAGATGC CGTCGGTGTG GGTGGACAGC TGGTCTCCCT TACTGAGCAG
  181 GAACACAGCC CCCTGGTACA TTGAGCGCAC CCACGGTCCT TGAAGTCCCG GATACACAGA
  241 CTTCTGCGCA CTGAGGAGAG GCACATGGAA GGGGTATTGG GAGGAAAAGA GCTGGACCTC
  301 GTGTGCCAGG TAGATGGGAG TGGGAATGGC CCTGGGGGAG CAGCTTTCTC CAGAGAAAAC
  361 CACCTGGGAG TAGACAAAGT AGAGGCCACT GGTGGGGATC AGGAGGGAGT TGTTGCTCAA
  421 AGAGAAGCCA TGTCGGAGAA AGGCACGATC CGTGCTTGCT CTCCAGAGCA GTGAGTTCTG
  481 CTTGCTGGGG TACCCAACAA GGTGAGCAGC AGGTTTCAGG ATGCCATGGG TCAAGTGCTT
  541 CTGAGGGAGT GGATGGGCTG TCCTGGCAGC GGAGAAGCGG ACACCAGAGA GTCCCTGGGC
  601 CCCTAGAGGC AGGGCCAGCA GCAGCCCCAG GAGGAAGACA GGAGGGGTGC CAAGCACCCT
  661 CAAGAGGTGG AGACGGCCGA GCAGTGTCAT GGTGTCTAGA ATTCGCTTTC CAGTCGGGAA
  721 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA
  781 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC
  841 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA CGGGATAACG
  901 CAGGAAAGAA CATGTGAGCA AAAAGGCCAG CCAAAAGGCC AGGGAACCCG TAAAAAGGCC
  961 GCGTTTGCCT GGCCGTTTTT TCCATAGGCC TCCGCCCCCC CCTGACGAGC CATCACCAAA
1021 AATCGACGCC TCCAAAGTCA AGAGGTGGCC GGAAACCCCG ACCAGGACCT ATAGGATACC
1081 AGCCGCTTTC CCCCTTGGAA GGCCTCTTCG ATGACGACCT TCCATCCCCA TG
//

Please visit Sequencing and PCR primers for primer information.