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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBluescript SKII+mIL7 (#RDB01475)

Mouse IL-7 cDNA

Alternative name Mouse IL7 cDNA
Clone info. Mouse IL7 cDNA.
Comment This clone contains a cDNA fragment corresponding to the 581nt to 1045nt of NM_008371.5. Known differences (at least) : A to T substitution at 739nt (Gly) ; T to C substitution at 742nt (Thr).
Vector backbone pBluescript II SK (+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Mouse IL-7 cDNA
Depositor|Developer Hamada, Hirofumi |
 
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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01475 pBluescript SKII+mIL7 DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBluescript SKII+mIL7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01475).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12743_A4Cpp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: REVERSE-2 (Pr0100)
Region: T3 pro,insert,T7 pro
Sequence file: RDB12743_A4Cpa.seq check
>01475_12743_A4Cp_Reverse_2_B04_04.ab1
    1 NNNNNNNNNN NNNNNNCNNG CGCGNNNTTA ACCCTCNCTA AAGGGAACAA AAGCTGGAGC
   61 TCCACCGCGG TGGCGGCCGC TCTAGACCAT GTTCCATGTT TCTTTTAGAT ATATCTTTGG
  121 AATTCCTCCA CTGATCCTTG TTCTGCTGCC TGTCACATCA TCTGAGTGCC ACATTAAAGA
  181 CAAAGAAGGT AAAGCATATG AGAGTGTACT GATGATCAGC ATCGATGAAT TGGACAAAAT
  241 GACAGGTACC GATAGTAATT GCCCGAATAA TGAACCAAAC TTTTTTAGAA AACATGTATG
  301 TGATGATACA AAGGAAGCTG CTTTTCTAAA TCGTGCTGCT CGCAAGTTGA AGCAATTTCT
  361 TAAAATGAAT ATCAGTGAAG AATTCAATGT CCACTTACTA ACAGTATCAC AAGGCACACA
  421 AACACTGGTG AACTGCACAA GTAAGGAAGA AAAAAACGTA AAGGAACAGA AAAAGAATGA
  481 TGCATGTTTC CTAAAGAGAC TACTGAGAGA AATAAAAACT TGTTGGAATA AAATTTTGAA
  541 GGGCAGTATA TAAACAGGAT CCCCCGGGCT GCAGGAATTC GATATCAAGC TTATCGATAC
  601 CGTCGACCTC GAGGGGGGGC CCGGTACCCA ATTCGCCCTA TAGTGAGTCG TATTACGCGC
  661 GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA
  721 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAANAG GCCCGCACCG
  781 ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GGANGCGCCC TGTAGCGGCG
  841 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGNGNN CGCTACACTT GCCAGCGCCC
  901 NANCGCCCGC NCCTTTCGCT TTCTNCCCNN CCTTTCNCGC CACGTNCGCC GGCTTTCCCN
  961 NNNNNGCNCT AAATCNGGGG NNNNCNNTTA NGNNNNCNNN TTNNNGCTTN ANNNNNNCNN
1021 NNNNCNNNAA AANNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles