Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMFG SV40tsT (#RDB01466)

Retroviral vector of SV40tsT

Clone info. Retroviral vector of SV40tsT.
Vector backbone pMFG (Plasmid)
Selectable markers Amp^r
Gene/insert name SV40 temperature sensitive largeT (tsT) cDNA
Depositor|Developer Hamada, Hirofumi |
 
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01466 pMFG SV40tsT DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMFG SV40tsT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01466).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12348_A4A6.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB12348_A4A6a.seq check
>01466_12348_A4A6_pMFG-1_A03_03.ab1
    1 GTCCATGGGA GAGGGGCTGT TAGTAACTCT TGTCATGTCA GGGTCAGGGA AGTTTACAAG
   61 GGGCGGGGGT GCCCCATATA AGATCTCATA TGGCCATATG GGGGCGCCTA GAGAAGGAGT
  121 GAGGGCTGGA TAAAGGGAGG ATCGAGGCGG GGTCGAACGA GGAGGTTCAA GGGGGAGAGA
  181 CGGGGCGGAT GGAGGAAGAG GAGGCGGAGG CTTAGGGTGT ACAAAGGGCT TGACCCAGGG
  241 AGGGGGGTCA AAAGCCAAGG CTTCCCAGGT CACGATGTAG GGGACCTGGT CTGGGTGTCC
  301 ATGCGGGCCA GGTGAAAAGA CCTTGATCTT AACCTGGGTG ATGAGGTCTC GGTTAAAGGT
  361 GCCGTCTCGC GGCCATCCGA CGTTAAAGGT TGGCCATTCT GCAGAGCAGA AGGTAACCCA
  421 ACGTCTCTTC TTGACATCTA CCGACTGGTT GTGAGCGATC CGCTCGACAT CTTTCCAGTG
  481 ACCTAAGGTC AAACTTAAGG GAGTGGTAAC AGTCTGGCCC GGGCCCATAT TTTCAGACAA
  541 ATACAGAAAC ACAGTCAGAC AGAGACAACA CAGAACGATG CTGCAGCAGA CAAGACGCGC
  601 GGCGCGGCTT CGGTCCCAAA CCGAAAGCAA AAATTCAGAC GGAGGCGGGA ACTGTTTTAG
  661 GTTCTCGTCT CCTACCAGAA CCACATATCC CTCCTCTAAG GGGGGTGCAC CAAAGAGTCC
  721 AAAACGATCG GGATTTTTGG ACTCAGGTCG GGCCACAAAA ACGGCCCCCG AAGTCCCTGG
  781 GACGTCTCCC AGGGTTGCGG CCGGGTGTTC CGAACTCGTC AGTTCCACCA CGGGTCCGCC
  841 AGATACAGAG CTAGTTAGCT AACTAGTACC GACGCAGGCG CATAAAATCA GTCATAGACA
  901 CTAGACAATC GGACAGACAC AGATAAGTTG CTGGCCAGCT TACCTCCCGG TGGTGGGTCG
  961 GTGGTCCCTG GGCAGGGGTC TCCCGATCCC GGGACGAGCC CCCAAATGAA AGACCCCCGC
 1021 TGACGGGTAG TCAATCACTC AGAGGGAGAC CCTCCCAAGG GAACAGCGAG ACCACAAGTC
 1081 GGAATGCAAC TGCAAGAGGA TTTATTGCAA TAACACGGGG TAACCTGGGT CGGACTTCAG
1141 TCCAATCCGG AGGACTGACG CGCCGAGTGA AGGGGGGATG TGGTGGGGCC TCCAT
//
Primer:
Sequence file: RDB12348_A4A6b.seq check
>01466_12348_A4A6_pMFG-F_C03_09.ab1
    1 GTTTTATTTG GGGGCAACCC CCGCCCCTTG TAACTTCCCT GACCCTGACA TGACAAGAGT
   61 TACTAACAGC CCCTCTCTCC AAGCTCACTT ACAGGCTCTC TACTTAGTCC AGCACGAAGT
  121 CTGGAGACCT CTGGCGGCAG CCTACCAAGA ACAACTGGAC CGACCGGTGG TACCTCACCC
  181 TTACCGAGTC GGCGACACAG TGTGGGTCCG CCGACACCAG ACTAAGAACC TAGAACCTCG
  241 CTGGAAAGGA CCTTACACAG TCCTGCTGAC CACCCCCACC GCCCTCAAAG TAGACGGCAT
  301 CGCAGCTTGG ATACACGCCG CCCACGTGAA GGCTGCCGAC CCCGGGGGTG GACCATCCTC
  361 TAGACTGCCA TGGATAAAGT TTTAAACAGA GAGGAATCTT TGCAGCTAAT GGACCTTCTA
  421 GGTCTTGAAA GGAGTGCCTG GGGGAATATT CCTCTGATGA GAAAGGCATA TTTAAAAAAA
  481 TGCAAGGAGT TTCATCCTGA TAAAGGAGGA GATGAAGAAA AAATGAAGAA AATGAATACT
  541 CTGTACAAGA AAATGGAAGA TGGAGTAAAA TATGCTCATC AACCTGACTT TGGAGGCTTC
  601 TGGGATGCAA CTGAGATTCC AACCTATGGA ACTGATGAAT GGGAGCAGTG GTGGAATGCC
  661 TTTAATGAGG AAAACCTGTT TTGCTCAGAA GAAATGCCAT CTAGTGATGA TGAGGCTACT
  721 GCTGACTCTC AACATTCTAC TCCTCCAAAA AAGAAGAGAA AGGTAGAAGA CCCCAAGGAC
  781 TTTCCTTCAG AATTGCTAAG TTTTTTGAGT CATGCTGTGT TTAATAATAA AACTCTTGCT
  841 TGCTTTGCTA TTTACACCAC AAAGGAAAAA GCTGCACTGC TATACAAGAA AATTATGGAA
  901 AAATATTCTG TAACCTTTAT AAGTAGGCAT AACAGTTATA ATCATAACAT ACTGTTTTTT
  961 TCTTACTCCA CACAGGCATA GAGTGTCTGC TATTAATAAC TATGCTCAAA AATTTGTGTA
 1021 CCTTTAGCTT TTTAATTTGT AAAGGGGTTT ATAAAGAATA TTTGATGTAA TAGTGCCCTT
 1081 GACTAGAAGA TCCCATTTTT CTGTTATTGA GGAAAGTTGC AAGTGGGTTA AGAAGCATGG
1141 ATTTTTAATC CTAGAAGGAA AAGCCAG
//
Primer:
Sequence file: RDB12348_A4A6c.seq check
>01466_12348_A4A6_pMFG-R_D03_12.ab1
    1 AGGGCATATC CGGGATCGAC ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA
   61 GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG CTGATTATGA
  121 TCATGAACAG ACTGTGAGGA CTGAGGGGCC TGAAATGAGC CTTGGGACTG TGAATCAATG
  181 CCTGTTTCAT GCCCTGAGTC TTCCATGTTC TTCTCCCCAC CATCTTCATT TTTATCAGCA
  241 TTTTCCTGGC TGTCTTCATC ATCATCATCA CTGTTTCTTA GCCAATCTAA AACTCCAATT
  301 CCCATAGCCA CATTAAACTT CATTTTTTGA TACACTGACA AACTAAACTC TTTGTCCAAT
  361 CTCTCTTTCC ACTCCACAAT TCTGCTCTGA ATACTTTGAG CAAACTCAGC CACAGGTCTG
  421 TACCAAATTA ACATAAGAAG CAAAGCAATG CCACTTTGAA TTATTCTCTT TTCTAACAAA
  481 AACTCACTGC GTTCCAGGCA ATGCTTTAAA TAATCTTTGG GCCTAAAATC TATTTGTTTT
  541 ACAAATCTGG CCTGCAGTGT TTTAGGCACA CTGTACTCAT TCATGGTGAC TATTCCAGGG
  601 GGAAATATTT GAGTTCTTTT ATTTAGGTGT TTCTTTTCTA AGTTTACCTT AACACTGCCA
  661 TCCAAATAAT CCCTTAAATT GTCCAGGTTA TTAATTCCCT GACCTGAAGG CAAATCTCTG
  721 GACTCCCCTC CAGTGCCCTT TACATCCTCA AAAACTACTA AAAACTGGTC AATAGCTACT
  781 CCTAGCTCAA AGTTCAGCCT GTCCAAGGGC AAATTAACAT TTAAAGCTTT CCCCCCACAT
  841 AATTCAAGCA AAGCAGCTGC TAATGTAGTT TTACCACTAT CAATTGGTCC TTTAAACAGC
  901 CAGTATCTTT TTTTTAGGAA TGTTGTACAT CATGCATTTT AAAAAGTCAT ACACCACTGA
  961 ATCCATTTTG GGCAACAAAC AGTGTAGCCC AAGCAACTCC CAGCCATCCA TTCTTTCTAT
 1021 GTCAGCAGAG CCTGTAGAAC CAAACATTTA TATCCCATCC CTATCCAAAA GATCATTAAA
 1081 TCTGTTTTGG TACATTTGTT CTTCTAGTAA TTGTAGGCTA TCAACCGCTT TTTAGCCTAA
1141 ACAGTAATCA ACCAGTCCTG GTTGGCCAAT TATTGGGTAT
//
Primer:
Sequence file: RDB12348_A4A6d.seq check
>01466_12348_A4A6_pMFG-2_B03_06.ab1
    1 ATCACCAATA TCACCAGCTG AGCCTATAGA GTACGAGCCA TAGATAAAAT AAAAGATTTT
   61 ATTTAGTCTC CAGAAAAAGG GGGGAATGAA AGACCCCACC TGTAGGTTTG GCAAGCTAGC
  121 TTAAGTAACG CCATTTTGCA AGGCATGGAA AAATACATAA CTGAGAATAG AGAAGTTCAG
  181 ATCAAGGTCA GGAACAGATG GAACAGCTGA ATATGGGCCA AACAGGATAT CTGTGGTAAG
  241 CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC AGATGGAACA GCTGAATATG GGCCAAACAG
  301 GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA AGAACAGATG GTCCCCAGAT
  361 GCGGTCCAGC CCTCAGCAGT TTCTAGAGAA CCATCAGATG TTTCCAGGGT GCCCCAAGGA
  421 CCTGAAATGA CCCTGTGCCT TATTTGAACT AACCAATCAG TTCGCTTCTC GCTTCTGTTC
  481 GCGCGCTTCT GCTCCCCGAG CTCAATAAAA GAGCCCACAA CCCCTCACTC GGGGCGCCAG
  541 TCCTCCGATT GACTGAGTCG CCCGGGTACC CGTGTATCCA ATAAACCCTC TTGCAGTTGC
  601 ATCCGACTTG TGGTCTCGCT GTTCCTTGGG AGGGTCTCCT CTGAGTGATT GACTACCCGT
  661 CAGCGGGGGT CTTTCACATG CAGCATGTAT CAAAATTAAT TTGGTTTTTT TTCTTAAGTA
  721 TTTACATTAA ATGGCCATAG TACTTAAAGT TACATTGGCT TCCTTGAAAT AAACATGGAG
  781 TATTCAGAAT GTGTCATAAA TATTTCTAAT TTTAAGATAG TATCTCCATT GGCTTTCTAC
  841 TTTTTCTTTT ATTTTTTTTT TGTCCTCTGT CTTCCATTTG TTGTTGTTGT TGTTTGTTTG
  901 TTTGTTTGTT GGTTGGTTGG TTAATTTTTT TTTAAAGATC CTACACTATA GTTCAAGCTA
  961 GACTATTAGC TACTCTGTAA CCCAGGGTGA CCTTGAAAGT CATGGGTAGC CTGCTGTTTA
 1021 GCCTTTCCCA CATCTAAGAT TACAGGTATG AGCTATCATT TTTGGGTATA TGATGATGAA
 1081 TTGAATTGAT GTGTGTGTGT GTGATGTGAT GTGTGGTGTG AATTGTGTAT ATGAGTGGGT
1141 ATATGGGCTT GGGGGGGTGG A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report TAN Yajun, Relationship between the P53 functional status of human tumor cell lines and their chemosensitivities to different anticancer drug. Chin. Edu. Gen. Pract., 4 (2006). [link to RRC of NBRP]