RDB01427 - pCA HSVtK

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pCA HSVtK (#RDB01427)

pCAcctk Herpes simplex virus-tk gene expression

Alternative name	pCAcctk
Clone info.	Expression vector of simplex herpes virus tk gene in ammalian cells
Vector backbone	pCAGGS (Plasmid)
Size of vector backbone	4.8 kb
Selectable markers	Amp^r (E. coli)
Gene/insert name	HSV thymidine kinase Genomic DNA
Depositor\|Developer	Hamada, Hirofumi \|

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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01427	pCA HSVtK	DNA solution	Please contact us.

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCA HSVtK was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01427).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB16749_A8Jcp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CAG_R1 (Pr0584) Region: CAC pro,insert 5'(HSV TK 5'-UTR,HSV TK CDS 5') Sequence file: RDB16749_A8Jce.seq
>01427_16749_A8Jc_CAG_R1_C02_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNC CNTCNAGCGG GCGCGGGGCG AAGCGGTGCG GCGCCGGCAG 61 GAAGGAAATG GGCGGGGAGG GCCTTCGTGC GTCGCCGCGC CGCCGTCCCC TTCTCCCTCT 121 CCAGCCTCGG GGCTGTCCGC GGGGGGACGG CTGCCTTCGG GGGGGACGGG GCAGGGCGGG 181 GTTCGGCTTC TGGCGTGTGA CCGGCGGCTC TAGAGCCTCT GCTAACCATG TTCATGCCTT 241 CTTCTTTTTC CTACAGCTCC TGGGCAACGT GCTGGTTATT GTGCTGTCTC ATCATTTTGG 301 CAAAGAATTG ATCTTGGTGG CGTGAAACTC CCGCACCTCT TCGGCAAGCG CCTTGTAGAA 361 GCGCGTATGG CTTCGTACCC CTGCCATCAA CACGCGTCTG CGTTCGACCA GGCTGCGCGT 421 TCTCGCGGCC ATAGCAACCG ACGTACGGCG TTGCGCCCTC GCCGGCAGCA AGAAGCCACG 481 GAAGTCCGCC TGGAGCAGAA AATGCCCACG CTACTGCGGG TTTATATAGA CGGTCCTCAC 541 GGGATGGGGA AAACCACCAC CACGCAACTG CTGGTGGCCC TGGGTTCGCG CGACGATATC 601 GTCTACGTAC CCGAGCCGAT GACTTACTGG CAGGTGCTGG GGGCTTCCGA GACAATCGCG 661 AACATCTACA CCACACAACA CCGCCTCGAC CAGGGTGAGA TATCGGCCGG GGACGCGGCG 721 GTGGTAATGA CAAGCGCCCA GATAACAATG GGCATGCCTT ATGCCGTGAC CGACGCCGTT 781 CTGGCTCCTC ATATCGGGGG GGANGCTGGG AGCTCACATG CCCCGCCCCC GGCCNNCACC 841 CTCATCTTCN ANCGCCATCC CATCGCCGCC CTCCNGTGCT ACNCGGCCGC GCGATACCTT 901 ATGGGCAGCA TGNNCCCCCC AGGCCGNGCT GGCGTTCGNN GCCNTNATCC CNCCNANCTT 961 GCCCNGNNNC AAANNTCNNN TNGGGGGGNC NNTNCNGANN NNANNNACAT NNNNNNNNNN //
Primer: pCAGGS_R1 (Pr0614) Region: bgh pA,insert 3'(HSV genome seq) Sequence file: RDB16749_A8Jcf.seq
>01427_16749_A8Jc_pCAGGS_R1_D02_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNCNC TNNNGNNNTG AAANTAAATT TCCTTTATTA GCCAGAAGTC 61 AGATGCTCAA GGGGCTTCAT GATGTCCCCA TAATTTTTGG CAGAGGGAAA AAGATCTCAG 121 TGGTATTTGT GAGCCAGGGC ATTGGCCACA CCAGCCACCA CCTTCTGATA GGCAGCCTGC 181 ACCTGAGGAG TGAATTGATC TATAGATCAT GAGTGGGAGG AATGAGCTGG CCCTTAATTT 241 GGTTTTGCTT GTTTAAATTA TGATATCCAA CTATGAAACA TTATCATAAA GCAATAGTAA 301 AGAGCCTTCA GTAAAGAGCA GGCATTTATC TAATCCCACC CCACCCCCAC CCCCGTAGCT 361 CCAATCCTTC CATTCAAAAT GTAGGTACTC TGTTCTCACC CTTCTTAACA AAGTATGACA 421 GGAAAAACTT CCATTTTAGT GGACATCTTT ATTGTTTAAT AGATCATCAA TTTCTGCAGG 481 TCCTGGCCGG GGTCCCGCGG AAACTCGGCC GTGGTGACCA ATACAAAACA AAAGCGCTCC 541 TCGTACCAGC GAAGAAGGGG CAGAGATGCC GTAGTCAGGT TTAGTTCGTC CGGCGGCGCC 601 AGAAATCCGC GCGGTGGTTT TTGGGGGTCG GGGGTGTTTG GCAGCCACAG ACGCCCGGTG 661 TTCGTGTCGC GCCAGTACAT GCGGTCCATG CCCAGGCCAT CCAAAAACCA TGGGTCTGTC 721 TGCTCAGTCC AGTCGTGGAN CTGNCCCCAC GCAACGCCCA AAATAATAAC CCCCACGAAC 781 CATAAACCAT TCCCCATGGG GGANCCCGTC CCTAACCCAC GGGGCCNGTG GCTATGGCAG 841 GGCTTGCCGC CCCGACGTTG GCTGCGAGCC CTNGGCCTTC ACCCGAACTT GGGGGGNGGG 901 GNGGGGAAAA GGAANAAACG CGGGCGTATT GGCCCCNATG GGGNCNCNGN GNNNATCGNN 961 ANAGTGCCAG CCCTGGGNNC GANNCCNNNN TTTATGNNNN ANNNANCCNN CNCCCNNNNN 1021 NTNTNNNNNN NNNTTTNNNN NNNNNN //
Primer: HSVTKup_f (Pr0736) Region: insert mid(HSV TK CDS 3') Sequence file: RDB16749_A8Jcg.seq
>01427_16749_B2L7F_HSVTKup_F_B01_02_ABI08.ab1 1 NNNNNNNNNN TTTNNNNNNN NNTTTTATTG CCNNCATAGC GCGGGTTCCT TCCGGTATTG 61 TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC ATCTCCCGGG CAAACGTGCG CGCCAGGTCG 121 CAGATCGTCG GTATGGAGCC GGGGGTGGTG ACGTGGGTCT GGACCATCCC GGAGGTAAGT 181 TGCAGCAGGG CGTCCCGGCA GCCGGCGGGC GATTGGTCGT AATCCAGGAT AAAGACGTGC 241 ATGGGACGGA GGCGTTTGGC CAAGACGTCC AAGGCCCAGG CAAACACGTT GTACAGGTCG 301 CCGTTGGGGG CCAGCAACTC GGGGGCCCGA AACAGGGTAA ATAACGTGTC CCCGATATGG 361 GGTCGTGGGC CCGCGTTGCT CTGGGGCTCG GCACCCTGGG GCGGCACGGC CGTCCCCGAA 421 AGCTGTCCCC AATCCTCCCG CCACGACCCG CCGCCCTGCA GATACCGCAC CGTATTGGCA 481 AGCAGCCCGT AAACGCGGCG AATCGCGGCC AGCATAGCCA GGTCAAGCCG CTCGCCGGGG 541 CGCTGGCGTT TGGCCAGGCG GTCGATGTGT CTGTCCTCCG GAANGNNNNC CAACACGATG 601 TTTGTGCCGG GCAAGGTCGG CGGGATGAGG GCCACNAACG CCANNNNNGC CTGNGGGGTC 661 NNNNNNCCCA TAANGTATCG CGCGGCCGGG TAGCACAGGA NGGCGGCGAT NNNATGNNNG 721 TCGAANANGA NGNTGAGGNC CNGGGGGCNN NGCATGTGAN NTNNCANCCT CCNNCCNNNN 781 ANATNANNAG CCNNAANNNN NNNNNN //