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pCA HSVtK

pCAcctk Herpes simplex virus-tk gene expression

Catalog number RDB01427
Resource name pCA HSVtK
Alternative name pCAcctk
Clone info. Expression vector of simplex herpes virus tk gene in ammalian cells
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r (E. coli)
Gene/insert name HSV thymidine kinase Genomic DNA
Depositor Hamada, Hirofumi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01427 pCA HSVtK DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01427.gif from Depositor

Featured content

Featured content Suicide gene therapy (English text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16749_A8Jcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR322_BamhI_F RDB16749_A8Jca.seq
>01427_16749_A8Jc_CAG_R1_C02_08_ABI24.ab1
    1 CATGGGAGCG CGCCGCACCC CCTCTAGCGG GCGCGGGGCG AAGCGGTGCG GCGCCGGCAG
   61 GAAGGAAATG GGCGGGGAGG GCCTTCGTGC GTCGCCGCGC CGCCGTCCCC TTCTCCCTCT
  121 CCAGCCTCGG GGCTGTCCGC GGGGGGACGG CTGCCTTCGG GGGGGACGGG GCAGGGCGGG
  181 GTTCGGCTTC TGGCGTGTGA CCGGCGGCTC TAGAGCCTCT GCTAACCATG TTCATGCCTT
  241 CTTCTTTTTC CTACAGCTCC TGGGCAACGT GCTGGTTATT GTGCTGTCTC ATCATTTTGG
  301 CAAAGAATTG ATCTTGGTGG CGTGAAACTC CCGCACCTCT TCGGCAAGCG CCTTGTAGAA
  361 GCGCGTATGG CTTCGTACCC CTGCCATCAA CACGCGTCTG CGTTCGACCA GGCTGCGCGT
  421 TCTCGCGGCC ATAGCAACCG ACGTACGGCG TTGCGCCCTC GCCGGCAGCA AGAAGCCACG
  481 GAAGTCCGCC TGGAGCAGAA AATGCCCACG CTACTGCGGG TTTATATAGA CGGTCCTCAC
  541 GGGATGGGGA AAACCACCAC CACGCAACTG CTGGTGGCCC TGGGTTCGCG CGACGATATC
  601 GTCTACGTAC CCGAGCCGAT GACTTACTGG CAGGTGCTGG GGGCTTCCGA GACAATCGCG
  661 AACATCTACA CCACACAACA CCGCCTCGAC CAGGGTGAGA TATCGGCCGG GGACGCGGCG
  721 GTGGTAATGA CAAGCGCCCA GATAACAATG GGCATGCCTT ATGCCGTGAC CGACGCCGTT
  781 CTGGCTCCTC ATATCGGGGG GGAGGCTGGG AGCTCACATG CCCCGCCCCC GGCCCTCACC
  841 CTCATCTTCG ACCGCCATCC CATCGCCGCC CTCCTGTGCT ACCCGGCCGC GCGATACCTT
  901 ATGGGCAGCA TGACCCCCCC AGGCCGTGCT GGCGTTCGTG GCCCTCATCC CGCCGACCTT
  961 GCCCGGGCAC AAACATCGTG TTGGGGGGCC CTTCCGGAGG ACAGACACAT CGACCGCCTG
 1021 GGCCAAACGC CAGCGCCCCC GGCGAGCGGG CTTTGACCCT GGCTATGCTG GGCGGCGGAT
 1081 TCGCGCGTTT ACGGGCTGCC TTGCATACCG GTGCGGTATC TGCAAGGCCG GCGGGTCGTT
1141 GGCCGGGGAA A
//
Primer: HSV_TK_pA_R RDB16749_A8Jcb.seq
>01427_16749_A8Jc_pCAGGS_R1_D02_11_ABI24.ab1
    1 GCCAGAAAAT TCCAACACAC TATTGCAATG AAAATAAATT TCCTTTATTA GCCAGAAGTC
   61 AGATGCTCAA GGGGCTTCAT GATGTCCCCA TAATTTTTGG CAGAGGGAAA AAGATCTCAG
  121 TGGTATTTGT GAGCCAGGGC ATTGGCCACA CCAGCCACCA CCTTCTGATA GGCAGCCTGC
  181 ACCTGAGGAG TGAATTGATC TATAGATCAT GAGTGGGAGG AATGAGCTGG CCCTTAATTT
  241 GGTTTTGCTT GTTTAAATTA TGATATCCAA CTATGAAACA TTATCATAAA GCAATAGTAA
  301 AGAGCCTTCA GTAAAGAGCA GGCATTTATC TAATCCCACC CCACCCCCAC CCCCGTAGCT
  361 CCAATCCTTC CATTCAAAAT GTAGGTACTC TGTTCTCACC CTTCTTAACA AAGTATGACA
  421 GGAAAAACTT CCATTTTAGT GGACATCTTT ATTGTTTAAT AGATCATCAA TTTCTGCAGG
  481 TCCTGGCCGG GGTCCCGCGG AAACTCGGCC GTGGTGACCA ATACAAAACA AAAGCGCTCC
  541 TCGTACCAGC GAAGAAGGGG CAGAGATGCC GTAGTCAGGT TTAGTTCGTC CGGCGGCGCC
  601 AGAAATCCGC GCGGTGGTTT TTGGGGGTCG GGGGTGTTTG GCAGCCACAG ACGCCCGGTG
  661 TTCGTGTCGC GCCAGTACAT GCGGTCCATG CCCAGGCCAT CCAAAAACCA TGGGTCTGTC
  721 TGCTCAGTCC AGTCGTGGAC CTGACCCCAC GCAACGCCCA AAATAATAAC CCCCACGAAC
  781 CATAAACCAT TCCCCATGGG GGACCCCGTC CCTAACCCAC GGGGCCCGTG GCTATGGCAG
  841 GGCTTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG
901 GGTGGGGGAA AAGGAAGA
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.11.01

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