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pAxCAmIL12p40 (#RDB01416)

Shuttle vector to generate rAd expressing mouse IL-12 p40

Alternative name pAdex1CAmIL12p40
Clone info. Shuttle vector to generate recombinant adenovirus expressing mouse IL-12p40 gene.
Comment Produce adenovirus by COS-TPC method.
Vector backbone pAxcw (Cosmid, use packaging extracts for transforming E. coli host)
Size of vector backbone 42.5 kb
Selectable markers Amp^r
Gene/insert name Mouse IL-12 p40 cDNA
Depositor|Developer Hamada, Hirofumi |
 
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Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(J. Clin. Invest., 102, 1807-1814 (1988).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Clin. Invest., 102, 1807-1814 (1988).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01416 pAxCAmIL12p40 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAxCAmIL12p40 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01416).

Reference section:

Yasuda, H., Nagata, M., Arisawa, K., Yoshida, R., Fujihira, K., Okamoto, N., Moriyama, H., Miki, M., Saito, I., Hamada, H., Yokono, K., Kasuga, M., Local expression of immunoregulatory IL-12p40 gene prolonged syngeneic islet graft survival in diabetic NOD mice. J. Clin. Invest., 102, 1807-1814 (1988). PMID 9819366.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB01416_B2L1p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CAG_R1 (Pr0584)
Region: CAG pro,insert 5'
Sequence file: RDB01416_B2L1a.seq check
>01416_01416_B2L1_3_CAG_R1_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNAN CGGGCGCGGG GCGNNNCGGT GCGGCGCCGG CAGGAAGGAA
   61 ATGGGCGGGG NGGGCCTTCG TGCGTCGCCG CGCCGCCGTC CCCTTCTCCC TCTCCAGCCT
  121 CGGGGCTGTC CGCGGGGGGA CGGCTGCCTT CGGGGGGGAC GGGGCAGGGC GGGGTTCGGC
  181 TTCTGGCGTG TGACCGGCGG CTCTAGAGCC TCTGCTAACC ATGTTCATGC CTTCTTCTTT
  241 TTCCTACAGC TCCTGGGCAA CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA
  301 TTAGACGGCA TCGCAGCTTG GATACACGCC GCCCACGTGA AGGCTGCCGA CCCCGGGGGT
  361 GGACCATCCT CTAGACTGCC ATGGGATGTC CTCAGAAGCT AACCATCTCC TGGTTTGCCA
  421 TCGTTTTGCT GGTGTCTCCA CTCATGGCCA TGTGGGAGCT GGAGAAAGAC GTTTATGTTG
  481 TAGAGGTGGA CTGGACTCCC GATGCCCCTG GAGAAACAGT GAACCTCACC TGTGACACGC
  541 CTGAAGAAGA TGACATCACC TGGACCTCAG ACCAGAGACA TGGAGTCATA GGCTCTGGAA
  601 AGACCCTGAC CATCACTGTC AAAGAGTTTC TAGATGCTGG CCAGTACACC TGCCACAAAG
  661 GAGGCGAGAC TCTGAGCCAC TCACATCTGC TGCTCCACAA GAANGAAAAT GGAATTTGGT
  721 CCACTGAAAT TTTAAAAAAT TTCAAAAACA AGACTTTCCT GAAGTGTGAA GCACCNAATT
  781 ACTCCGGACG GTTCACGTGC TCATGGCTGG TGCNAAGAAA CATGGNCTTG ANNTTCAACA
  841 TCNAGAGCAG TANCAGTTCC CNNNNNTCTC GGGCAGNGNN ATGTGGAATN GCGTCTCNGN
  901 CNGCNNANNA NNNNCACACN NGNNNNNAAN GGNNTNNNAN AANGTATTCN GTGNNCNNNN
961 NNNNNN
//
Primer: pCAGGS_R1 (Pr0614)
Region: beta-globin polyA,insert 3'
Sequence file: RDB01416_B2L1b.seq check
>01416_01416_B2L1_3_pCAGGS_R1_G03_21_ABI24.ab1
    1 ANNNNNNNNN NNNNNACNAT TGNNNTGNAA NNAAATTTCC TTTATTAGCC AGAAGTCAGA
   61 TGCTCAAGGG GCTTCATGAT GTCCCCATAA TTTTTGGCAG AGGGAAAAAG ATCTCAGTGG
  121 TATTTGTGAG CCAGGGCATT GGCCACACCA GCCACCACCT TCTGATAGGC AGCCTGCACC
  181 TGAGGAGTGA ATTATCCTGT CTTTAACAAA TTGGACTAAT CCGGATCCTA GGATCGGACC
  241 CTGCAGGGAA CACATGCCCA CTTGCTGCAT GAGGAATTGT AATAGCGATC CTGAGCTTGC
  301 ACGCAGACAT TCCCGCCTTT GCATTGGACT TCGGTAGATG TCTTCTCTAC GAGGAACGCA
  361 CCTTTCTGGT TACACCCCTC CTCTGTCTCC TTCATCTTTT CTTTCTTGCG CTGGATTCGA
  421 ACAAAGAACT TGAGGGAGAA GTAGGAATGG GGAGTGCTCC AGGAGTCAGG GTACTCCCAG
  481 CTGACCTCCA CCTGTGAGTT CTTCAAAGGC TTCATCTGCA AGTTCTTGGG CGGGTCTGGT
  541 TTGATGATGT CCCTGATGAA GAAGCTGGTG CTGTAGTTCT CATATTTATT CTGCTGCCGT
  601 GCTTCCAACG CCAGTTCAAT GGGCAGGGTC TCCTCGGCAG TTGGGCAGGT GACATCCTCC
  661 TGGCAGGACA CTGAATACTT CTCATAGTCC CTTTGGTCCA GTGTGACCTT CTCTGCAGAC
  721 AGAGACGCCA TTCCACATGT CACTGCCCGA NAGTCANGNG AACTGCTACT GCTCTTGATG
  781 TTGAACTTCA AGTCCATGTT TCTTTGCACC AGCCATGANC ACGTGAACCG TCCGGANTAA
  841 TTNGGTGCTT CACACTTCNN NAAAGTCTTG NTTTTTGAAA TTTTTTTAAA ATTTCANNGG
  901 ANNNAATTNC ATTTTTCCTN NTNGNNGNAN CANCANANGN NNNNNNNNCA NANNNNCGNC
961 NNNCNNTNNN NNNNNNN
//
Primer: Amp_R (Pr0008)
Region: AmpR pro,ITR,Ad5 psi
Sequence file: RDB01416_B2L1c.seq check
>01416_01416_B2L1_3_Amp_R_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGGTTATTG TCTCATGAGC GGANACATAT TTGAATGTAT
   61 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT
  121 CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT
  181 TCGTCTTCAA GAATTCCGGA TCCGGGCCAA TATGATAATG AGGGGGTGGA GTTTGTGACG
  241 TGGCGCGGGG CGTGGGAACG GGGCGGGTGA CGTAGTAGTG TGGCGGAAGT GTGATGTTGC
  301 AAGTGTGGCG GAACACATGT AAGCGACGGA TGTGGCAAAA GTGACGTTTT TGGTGTGCGC
  361 CGGTGTACAC AGGAAGTGAC AATTTTCGCG CGGTTTTAGG CGGATGTTGT AGTAAATTTG
  421 GGCGTAACCG AGTAAGATTT GGCCATTTTC GCGGGAAAAC TGAATAAGAG GAAGTGAAAT
  481 CTGAATAATT TTGTGTTACT CATAGCGCGT AATATTTGTC TAGGGCCGCG GGGACTTTGA
  541 CCGTTTACGT GGAGACTCGC CCAGGTGTTT TTCTCAGGTG TTTTCCGCGT TCCGGGTCAA
  601 AGTTGGCGTT TTATTATTAT AGTCAGCATC GATGAGCTTG ATAGCTTGGG CTGCAGGTCG
  661 AGGGATCTCC ATAAGAGAAG AGGGACAGCT ATGACTGGGA GTAGTCAGGA GAGGAGGAAA
  721 AATCTGGCTA GTAAAACATG TAAGGAAAAT TTTAGGGATG TTAAAGAAAA AAAATAACAC
  781 AAAACAAAAT ATAAAAAAAA TCTAACCTCN AGTCAAGGCT TTTTCTATGG AATAANGANN
  841 GGNCAGCNNG GGGCTGTTTC ATATACTGAT GANCTCTTTA TAGCCAACCT TNNTNCATGG
  901 NAGCCAGCAT ATGGGNATAT GNTGNNNNNN NTNNNNNNAA NNNNNNTTNN NNNGNTTNNA
961 NNNNNTNNNC NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yasuda, H., Local expression of immunoregulatory IL-12p40 gene prolonged syngeneic islet graft survival in diabetic NOD mice. J. Clin. Invest., 102, 1807-1814 (1988). PMID 9819366.