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Human Neuroral NOS cDNA (#RDB01387)

Human HBNOS 3'-2 cDNA, a partial cDNA of NOS (ca. 0.7 kb).

Alternative name HB-NOS 3'-2
Clone info. Human HBNOS 3'-2 cDNA, a partial cDNA of NOS (ca. 0.7 kb).
Vector backbone pTZ19U (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name Human Nitric oxide synthase cDNA
Depositor|Developer Esumi, Hiroyasu | Fujisawa, Hironori |
 
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Distribution information

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01387 Human Neuroral NOS cDNA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Human Neuroral NOS cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01387).

Reference section:

Fujisawa, H., Ogura, T., Kurashima, Y., Yokoyama, T., Yamashita, J., Esumi, H., Expression of two types of nitric oxide synthase mRNA in human neuroblastoma cell lines. J. Neurochem., 63, 140-145 (1994). PMID 7515942. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17884_A9L9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_ori-N_R (Pr0275)
Region: f1 ori,insert5'
Sequence file: RDB17884_A9L9a.seq check
>01387_17884_A9L9_1_M13_ori-N_R_B03_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT NNNGCNNNNN NACNGGGCGC GTCCCATTCG
   61 CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC
  121 CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC
  181 CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAATTCTATG CTCCTCCTGG CTCAACCGGA
  241 TACAGGCTGA CGAACTGGTC CCCTGTTTCG TGAGAGGAGC ACCCAGCTTC CACCTGCCCC
  301 GGAACCCCCA AGTCCCCTGC ATCCTCGTTG GACCAGGCAC CGGCATTGCC CCTTTCCGAA
  361 GCTTCTGGCA ACAGCGGCAA TTTGATATCC AACACAAAGG AATGAACCCC TGCCCCATGG
  421 TCCTGGTCTT CGGGTGCCGG CAATCCAAGA TAGATCATAT CTACAGGGAA GAGACCCTGC
  481 AGGCCAAGAA CAAGGGGGTC TTCAGAGAGC TGTACACGGC TTACTCCCGG GAGCCAGACA
  541 AACCAAAGAA GTACGTGCAN NNCATCCTGC AGGAGCAGCT GGCGGAGTCT GTGTACCGAG
  601 CCCTGAAGGA GCAAGGGGGC CACATATACG TCTGTGGGGA CGTCACCATG GCTGCTGATG
  661 TCCTCAAAGC CATCCAGCGC ATCATGACCC AGCAGGGGAA GCTCTCGGCA GANGACGCCG
  721 GCGTATTCAT CAGCCGGATG ANGGATGACA ACCGATACCA TGANGANATT TTTGGAGTCA
  781 CCCTGCGAAC GTACGAAGTG ACCNACCGCC TTAGATCTGA NTCCATTGCC TTCATTGAAG
  841 AGAGCNAAAA AAGANACCGA TGNNGNTTTC AGCTCCNAAC NGGNNCCTCT TGCCNNGCNN
  901 GNNGCNNNTT TGTAANCGCG GGNNANANAA NTCNNNCTCN NNNNCNNGGG NNTNCNCTNN
961 NNNNNNNN
//
Primer: reverse-2 (Pr0100)
Region: MCS,insert3'
Sequence file: RDB17884_A9L9b.seq check
>01387_17884_A9LcF_1_reverse2_D08_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNCTA TAGGGNAAGC TTGCATGCCT
   61 GCAGGTCGAC TCTAGAGGNN CCCCGGGTAC CGAGCTCGAA TTCTCTGTCC CGCGCTTACA
  121 AACTTGCAGC CGGCTGGGCA AGAGGGTCCA GTTAGGAGCT GAAAACCTCA TCGGTGTCTT
  181 TTTTGCTCTC TTCAATGAAG GCAATGGACT CAGATCTAAG GCGGTTGGTC ACTTCGTACG
  241 TTCGCAGGGT GACTCCAAAA ATATCCTCAT GGTATCGGTT GTCATCCCTC ATCCGGCTGA
  301 TGAATACGCC GGCGTCCTCT GCCGAGAGCT TCCCCTGCTG GGTCATGATG CGCTGGATGG
  361 CTTTGAGGAC ATCAGCAGCC ATGGTGACGT CCCCACAGAC GTATATGTGG CCCCCTTGCT
  421 CCTTCAGGGC TCGGTACACA GACTCCGCCA GCTGCTCCTG CAGGATGTCC TGCACGTACT
  481 TCTTTGGTTT GTCTGGCTCC CGGGAGTAAG CCGTGTACAG CTCTCTGAAG ACCCCCTTGT
  541 TCTTGGCCTG CAGGGTCTCT TCCCTGTAGA TATGATCTAT CTTGGATTGC CGGCACCCGA
  601 AGACCAGGAC CATGGGGCAG GGGTTCATTC CTTTGTGTTG GATATCAAAT TGCCGCTGTT
  661 GCCAGAAGCT TCGGAAAGGG GCAATGCCGG TGCCTGGTCC AACGAGGANG CAGGGGNNTT
  721 GGGGGTTCCG GGGCAGGTGG AAGCTGGGTG CTCCTCTCAC GAAACAGGGG ANCAGTTCGT
  781 CAGCCTGTAT CCGGTTGANC CAGGANGANC ATAGAATTCA CTGGCCGTCG TTTTACAACG
  841 TCGNGNNNGG GAAAANCCTG GCGTTACCCN ACTTNNTCGC CTNNCNNCAC ATCCCCCCTT
  901 NNNCCNNCNG GNNNAATANC GNANNANNNN CNNNNNNNNT CGNCCNTNCC CNANCANNNG
961 NNNNNNNNGN ANNNNNNNAN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fujisawa, H., Expression of two types of nitric oxide synthase mRNA in human neuroblastoma cell lines. J. Neurochem., 63, 140-145 (1994). PMID 7515942. [link to RRC of NBRP]