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pMDRA1

Plasmid clone of human P-glycoprotein cDNA

Catalog number RDB01372
Resource name pMDRA1
Alternative name Human P-glycoprotein cDNA
Clone info. Plasmid clone of human P-glycoprotein cDNA.
Please refer Fig1. A of Biochem. Biophys. Res. Commun., 162, 224-231 (1989).
Vector backbone pBluescript KS (+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 cDNA
Depositor Ueda, Kazumitsu |
 
Other clones in our bank human ABCB1 (NCBI Gene 5243) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR This material is provided with written consent of Depositor. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Biochem. Biophys. Res. Commun., 162, 224-231 (1989)).
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Material Transfer Agreement (MTA) [Word]
Approval form by depositor [Word]
Remarks ((Additional form)) Approval Form(FormD)
Please obtain written approval of the depositor.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01372 pMDRA1 DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01372.gif from Depositor

Featured content

References

original Kioka, N., P-glycoprotein gene (MDR1) cDNA from human adrenal: normal P-glycoprotein carries Gly185 with an altered pattern of multidrug resistance. Biochem. Biophys. Res. Commun., 162, 224-231 (1989). PMID 2568832.
original Chen, C.J., Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells. Cell, 47, 381-389 (1986). PMID 2876781.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12371_A5Hsp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB12371_A5Hsa.seq
>01372_12371_A5Hs_M13_-40_A10_01.ab1
    1 TAGGCCAGTG AATTGTTATA CGACTCACTA TAGGGCGAAT TGGAGCTCCA CCGCGGTGGC
   61 ATTCGAGTAG CGGCTCTTCC AAGCTCAAAG AAGCAGAGGC CGCTGTTCGT TTCCTTTAGG
  121 TCTTTCCACT AAAGTCGGAG TATCTTCTTC CAAAATTTCA CGTCTTGGTG GCCGTTCCAA
  181 GGAGCGCGAG GTCGGGATGG ATCTTGAAGG GGACCGCAAT GGAGGAGCAA AGAAGAAGAA
  241 CTTTTTTAAA CTGAACAATA AAAGTGAAAA AGATAAGAAG GAAAAGAAAC CAACTGTCAG
  301 TGTATTTTCA ATGTTTCGCT ATTCAAATTG GCTTGACAAG TTGTATATGG TGGTGGGAAC
  361 TTTGGCTGCC ATCATCCATG GGGCTGGACT TCCTCTCATG ATGCTGGTGT TTGGAGAAAT
  421 GACAGATATC TTTGCAAATG CAGGAAATTT AGAAGATCTG ATGTCAAACA TCACTAATAG
  481 AAGTGATATC AATGATACAG GGTTCTTCAT GAATCTGGAG GAAGACATGA CCAGGTATGC
  541 CTATTATTAC AGTGGAATTG GTGCTGGGGT GCTGGTTGCT GCTTACATTC AGGTTTCATT
  601 TTGGTGCCTG GCAGCTGGAA GACAAATACA CAAAATTAGA AAACAGTTTT TTCATGCTAT
  661 AATGCGACAG GAGATAGGCT GGTTTGATGT GCACGATGTT GGGGAGCTTA ACACCCGACT
  721 TACAGATGAT GTCTCCAAGA TTAATGAAGG AATTGGTGAC AAAATTGGAA TGTTCTTTCA
  781 GTCAATGGCA ACATTTTTCA CTGGGTTTAT AGTAGGATTT ACACGTGGTT GGAAGCTAAC
  841 CCTTGTGATT TTGGCCATCA GTCCTGTTCT TGGACTGTCA GCTGCTGTCT GGGCAAAGAT
  901 ACTATCTTCA TTTACTGATA AAGAACTCTT AGCGTATGCA AAAGCTGGAG CAGTAGCTGA
  961 AGAGGTCTTG GCAGCAATTA GAACTGTGAT TGCATTTGGG AGGACAAAAG AAAGAACTTG
 1021 AAAGGTACAC AAAAATTTAG AAGAGCTAAA GAATTGGGAA TAAAGAAGCT ATACAGCCCA
 1081 ATATTTTCTA TAGGTGCTGC TTTCCTGCTG AATCTATGCA ATCTAATGGC TCTGGACATT
1141 CTGGTTATGG GGAACCAACC TTGGGTTGCT
//
Primer: Reverse2 RDB12371_A5Hsb.seq
>01372_12371_A5Hs_Reverse2_G09_21.ab1
    1 AGGTAACCTT GAAATTTCGG CCAAGCTCGA AATTAACCCT CACTAAAGGG AACAAAAGCT
   61 GGTACCGGGC CCCCCCTCGA GGTCGACGGT ATCGATAAGC TTGATATCGA ATTCCTGCAG
  121 CCCTTTTTTT TTTTTTTCCA GTCACATGAA AGTTTAGTTT TATTATAGAC ACTTTATGCA
  181 AACATTTCAA TACTTTTTGC TACTTCTATA ATCTTTTAGC AAGGCAGTCA GTTACAGTCC
  241 AAATGGGAAA ATATAAACAA AATTACACAT TTTATCTTTT AAAATCTACT TTAATTCTGT
  301 TATAAAATTT ATAATGCAGT TTAAACTACG ATTTCTCTCC ACTTGATGAT GTCTCTCACT
  361 CTGTTCCTTT AATTACGAAG TCTCTGAAGA CTCTGAACTT GACTGAGGAA ATGTTAAACA
  421 GATACCTCTT CATAATTCTG TAAGTGTTTG CTTTTAACTT TGAATAAATG TCATATCTAA
  481 ACAAATATTA AAAAGTATTT AACATCTCAT ACAGTCAGAG TTCACTGGCG CTTTGTTCCA
  541 GCCTGGACAC TGACCATTGA AAAATAGATG CCTTTCTGTG CCAGCAGCTG CTGATGCGTG
  601 CCATGCTCCT TGACTCTGCC ATTCTGAAAC ACCACTATTA AGTCTGCATT CTGGATGGTG
  661 GACAGGCGGT GAGCAATCAC AATGCAGGTG CGGCCTTCTC TGGCTTTGTC CAGGGCTTCT
  721 TGGACAACCT TTTCACTTTC TGTATCCAGA GCTGACGTGG CTTCATCCAA AAGCAAAATA
  781 TGAGGCTGTC TAACAAGGGC ACGAGCTATG GCAATGCGTT GTTTCTGGCC ACCAGAGAGC
  841 TGAGTTCCTT TGTCTCCTAC TTTAGTGCTA TATTTATTAG GCAGTGACTC GATGAAGGCA
  901 TGTATGTTGG CCTCCTTTGC TGCCCTCACA ATCTCTTCCT GTGACACCAC CCCGGCTGTT
  961 GTCTCCATAG GCAATGTTCT CAGCAATGCT GCAGTCAAAC AGGATGGGGC TCCCTGGGAC
 1021 ACGATGCCCA AGGTGTGCTC GGAGCCACTG AACATTCAAG TCGCTTTATT CTTTTGCATC
 1081 AGGCAGCACT TTCCCTTGCC AGGGGTCGTA GAACCGCCTC AAGAGCTGGA ACACTGTGCT
1141 CTCCCACAGC CACTGTCTGA TCCAACCAGA AGTCCAGCGC GTCTG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kioka, N., P-glycoprotein gene (MDR1) cDNA from human adrenal: normal P-glycoprotein carries Gly185 with an altered pattern of multidrug resistance. Biochem. Biophys. Res. Commun., 162, 224-231 (1989). PMID 2568832.
original Chen, C.J., Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells. Cell, 47, 381-389 (1986). PMID 2876781.
user report Teng, Y.N., beta-carotene reverses multidrug resistant cancer cells by selectively modulating human P-glycoprotein function. Phytomedicine 23 (3): 316-323, 2016. PMID 26969385.
user report Xiang, Q.F., Cabozantinib reverses multidrug resistance of human hepatoma HepG2/adr cells by modulating the function of P-glycoprotein. Liver Int. 35 (3): 1010-1023 (2015). PMID 24621440.
user report Teng, Y.N., Demethoxycurcumin Modulates Human P-Glycoprotein Function via Uncompetitive Inhibition of ATPase Hydrolysis Activity. J. Agric. Food Chem. 63 (3): 847-855 (2015). PMID 25594233.
user report Sheu, M.J., The functional influences of common ABCB1 genetic variants on the inhibition of P-glycoprotein by Antrodia cinnamomea extracts. PLoS One, 9 (2):e89622 (2014). PMID 24586917.
user report Hung, C.C., Functional impact of ABCB1 variants on interactions between P-glycoprotein and methadone. PLoS One. 2013;8(3):e59419. PMID 23527191.
user report Hung, C.C., YC-1, a novel potential anticancer agent, inhibit multidrug-resistant protein via cGMP-dependent pathway. Invest New Drugs 29 (6): 1337-1346 (2011). PMID 20676745.
user report Kugawa F., Construction of a model cell line for the assay of MDR1 (multi drug resistance gene-1) substrates/inhibitors using HeLa cells. Pharmazie, 64, 296-300 (2009). PMID 19530439.
user report Hung C.C., Functional evaluation of polymorphisms in the human ABCB1 gene and the impact on clinical responses of antiepileptic drugs. Pharmacogenet. Genomics, 18, 390-402 (2008). PMID 18408562.
user report Morita N., Human MDR1 polymorphism: G2677T/A and C3435T have no effect on MDR1 transport activities. Biochem. Pharmacol., 65, 1843-1852 (2003). PMID 12781336.
user report Fujise H., Transepithelial transport and cellular accumulation of steroid hormones and polychlorobiphenyl in porcine kidney cells expressed with human P-glycoprotein. Chemosphere., 46, 1505-1511 (2002). PMID 12002483.

2019.03.08

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