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pMDRA1

Plasmid clone of human P-glycoprotein cDNA

Catalog number RDB01372
Resource name pMDRA1
Alternative name Human P-glycoprotein cDNA
Clone info. Plasmid clone of human P-glycoprotein cDNA.
Comment Please refer Fig1. A of Biochem. Biophys. Res. Commun., 162, 224-231 (1989).
Vector backbone pBluescript KS (+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 cDNA
Depositor|Developer Ueda, Kazumitsu |
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Human ATP-binding cassette, sub-family B (MDR/TAP), member 1
          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB01372.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution This material is provided with written consent of Depositor. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Biochem. Biophys. Res. Commun., 162, 224-231 (1989)).
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form by depositor [Word]
          Remarks ((Additional form)) Approval Form(FormD)
          Please obtain written approval of the depositor.
          提供案内 (日本国内) [open/close]

          提供条件 寄託者に許可をとること。 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Biochem. Biophys. Res. Commun., 162, 224-231 (1989))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          寄託者による提供承諾書 [Word]
          備考 【追加提供依頼書】承諾書(FormD)
          寄託者の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB01372 pMDRA1 DNA solution Please contact us.

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pMDRA1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01372).

          Reference section:

          Kioka, N., Tsubota, J., Kakehi, Y., Komano, T., Gottesman, M.M., Pastan, I., Ueda, K., P-glycoprotein gene (MDR1) cDNA from human adrenal: normal P-glycoprotein carries Gly185 with an altered pattern of multidrug resistance. Biochem. Biophys. Res. Commun., 162, 224-231 (1989). PMID 2568832.
          Chen, C.J., Chin, J.E., Ueda, K., Clark, D.P., Pastan, I., Gottesman, M.M., Roninson, I.B., Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells. Cell, 47, 381-389 (1986). PMID 2876781.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12371_A5Hsp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB12371_A5Hsa.seq check
          >01372_12371_A5Hs_M13_-40_A10_01.ab1
              1 TAGGCCAGTG AATTGTTATA CGACTCACTA TAGGGCGAAT TGGAGCTCCA CCGCGGTGGC
             61 ATTCGAGTAG CGGCTCTTCC AAGCTCAAAG AAGCAGAGGC CGCTGTTCGT TTCCTTTAGG
            121 TCTTTCCACT AAAGTCGGAG TATCTTCTTC CAAAATTTCA CGTCTTGGTG GCCGTTCCAA
            181 GGAGCGCGAG GTCGGGATGG ATCTTGAAGG GGACCGCAAT GGAGGAGCAA AGAAGAAGAA
            241 CTTTTTTAAA CTGAACAATA AAAGTGAAAA AGATAAGAAG GAAAAGAAAC CAACTGTCAG
            301 TGTATTTTCA ATGTTTCGCT ATTCAAATTG GCTTGACAAG TTGTATATGG TGGTGGGAAC
            361 TTTGGCTGCC ATCATCCATG GGGCTGGACT TCCTCTCATG ATGCTGGTGT TTGGAGAAAT
            421 GACAGATATC TTTGCAAATG CAGGAAATTT AGAAGATCTG ATGTCAAACA TCACTAATAG
            481 AAGTGATATC AATGATACAG GGTTCTTCAT GAATCTGGAG GAAGACATGA CCAGGTATGC
            541 CTATTATTAC AGTGGAATTG GTGCTGGGGT GCTGGTTGCT GCTTACATTC AGGTTTCATT
            601 TTGGTGCCTG GCAGCTGGAA GACAAATACA CAAAATTAGA AAACAGTTTT TTCATGCTAT
            661 AATGCGACAG GAGATAGGCT GGTTTGATGT GCACGATGTT GGGGAGCTTA ACACCCGACT
            721 TACAGATGAT GTCTCCAAGA TTAATGAAGG AATTGGTGAC AAAATTGGAA TGTTCTTTCA
            781 GTCAATGGCA ACATTTTTCA CTGGGTTTAT AGTAGGATTT ACACGTGGTT GGAAGCTAAC
            841 CCTTGTGATT TTGGCCATCA GTCCTGTTCT TGGACTGTCA GCTGCTGTCT GGGCAAAGAT
            901 ACTATCTTCA TTTACTGATA AAGAACTCTT AGCGTATGCA AAAGCTGGAG CAGTAGCTGA
            961 AGAGGTCTTG GCAGCAATTA GAACTGTGAT TGCATTTGGG AGGACAAAAG AAAGAACTTG
           1021 AAAGGTACAC AAAAATTTAG AAGAGCTAAA GAATTGGGAA TAAAGAAGCT ATACAGCCCA
           1081 ATATTTTCTA TAGGTGCTGC TTTCCTGCTG AATCTATGCA ATCTAATGGC TCTGGACATT
          1141 CTGGTTATGG GGAACCAACC TTGGGTTGCT
          //
          Primer: Reverse2
          Sequence file: RDB12371_A5Hsb.seq check
          >01372_12371_A5Hs_Reverse2_G09_21.ab1
              1 AGGTAACCTT GAAATTTCGG CCAAGCTCGA AATTAACCCT CACTAAAGGG AACAAAAGCT
             61 GGTACCGGGC CCCCCCTCGA GGTCGACGGT ATCGATAAGC TTGATATCGA ATTCCTGCAG
            121 CCCTTTTTTT TTTTTTTCCA GTCACATGAA AGTTTAGTTT TATTATAGAC ACTTTATGCA
            181 AACATTTCAA TACTTTTTGC TACTTCTATA ATCTTTTAGC AAGGCAGTCA GTTACAGTCC
            241 AAATGGGAAA ATATAAACAA AATTACACAT TTTATCTTTT AAAATCTACT TTAATTCTGT
            301 TATAAAATTT ATAATGCAGT TTAAACTACG ATTTCTCTCC ACTTGATGAT GTCTCTCACT
            361 CTGTTCCTTT AATTACGAAG TCTCTGAAGA CTCTGAACTT GACTGAGGAA ATGTTAAACA
            421 GATACCTCTT CATAATTCTG TAAGTGTTTG CTTTTAACTT TGAATAAATG TCATATCTAA
            481 ACAAATATTA AAAAGTATTT AACATCTCAT ACAGTCAGAG TTCACTGGCG CTTTGTTCCA
            541 GCCTGGACAC TGACCATTGA AAAATAGATG CCTTTCTGTG CCAGCAGCTG CTGATGCGTG
            601 CCATGCTCCT TGACTCTGCC ATTCTGAAAC ACCACTATTA AGTCTGCATT CTGGATGGTG
            661 GACAGGCGGT GAGCAATCAC AATGCAGGTG CGGCCTTCTC TGGCTTTGTC CAGGGCTTCT
            721 TGGACAACCT TTTCACTTTC TGTATCCAGA GCTGACGTGG CTTCATCCAA AAGCAAAATA
            781 TGAGGCTGTC TAACAAGGGC ACGAGCTATG GCAATGCGTT GTTTCTGGCC ACCAGAGAGC
            841 TGAGTTCCTT TGTCTCCTAC TTTAGTGCTA TATTTATTAG GCAGTGACTC GATGAAGGCA
            901 TGTATGTTGG CCTCCTTTGC TGCCCTCACA ATCTCTTCCT GTGACACCAC CCCGGCTGTT
            961 GTCTCCATAG GCAATGTTCT CAGCAATGCT GCAGTCAAAC AGGATGGGGC TCCCTGGGAC
           1021 ACGATGCCCA AGGTGTGCTC GGAGCCACTG AACATTCAAG TCGCTTTATT CTTTTGCATC
           1081 AGGCAGCACT TTCCCTTGCC AGGGGTCGTA GAACCGCCTC AAGAGCTGGA ACACTGTGCT
          1141 CTCCCACAGC CACTGTCTGA TCCAACCAGA AGTCCAGCGC GTCTG
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Kioka, N., P-glycoprotein gene (MDR1) cDNA from human adrenal: normal P-glycoprotein carries Gly185 with an altered pattern of multidrug resistance. Biochem. Biophys. Res. Commun., 162, 224-231 (1989). PMID 2568832.
          original Chen, C.J., Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells. Cell, 47, 381-389 (1986). PMID 2876781.
          user_report Teng, Y.N., beta-carotene reverses multidrug resistant cancer cells by selectively modulating human P-glycoprotein function. Phytomedicine 23 (3): 316-323, 2016. PMID 26969385.
          user_report Xiang, Q.F., Cabozantinib reverses multidrug resistance of human hepatoma HepG2/adr cells by modulating the function of P-glycoprotein. Liver Int. 35 (3): 1010-1023 (2015). PMID 24621440.
          user_report Teng, Y.N., Demethoxycurcumin Modulates Human P-Glycoprotein Function via Uncompetitive Inhibition of ATPase Hydrolysis Activity. J. Agric. Food Chem. 63 (3): 847-855 (2015). PMID 25594233.
          user_report Sheu, M.J., The functional influences of common ABCB1 genetic variants on the inhibition of P-glycoprotein by Antrodia cinnamomea extracts. PLoS One, 9 (2):e89622 (2014). PMID 24586917.
          user_report Hung, C.C., Functional impact of ABCB1 variants on interactions between P-glycoprotein and methadone. PLoS One. 2013;8(3):e59419. PMID 23527191.
          user_report Hung, C.C., YC-1, a novel potential anticancer agent, inhibit multidrug-resistant protein via cGMP-dependent pathway. Invest New Drugs 29 (6): 1337-1346 (2011). PMID 20676745.
          user_report Kugawa F., Construction of a model cell line for the assay of MDR1 (multi drug resistance gene-1) substrates/inhibitors using HeLa cells. Pharmazie, 64, 296-300 (2009). PMID 19530439.
          user_report Hung C.C., Functional evaluation of polymorphisms in the human ABCB1 gene and the impact on clinical responses of antiepileptic drugs. Pharmacogenet. Genomics, 18, 390-402 (2008). PMID 18408562.
          user_report Morita N., Human MDR1 polymorphism: G2677T/A and C3435T have no effect on MDR1 transport activities. Biochem. Pharmacol., 65, 1843-1852 (2003). PMID 12781336.
          user_report Fujise H., Transepithelial transport and cellular accumulation of steroid hormones and polychlorobiphenyl in porcine kidney cells expressed with human P-glycoprotein. Chemosphere., 46, 1505-1511 (2002). PMID 12002483.

          2023.05.02

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