RDB01372 - pMDRA1

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pMDRA1

Plasmid clone of human P-glycoprotein cDNA

Catalog number	RDB01372
Resource name	pMDRA1
Alternative name	Human P-glycoprotein cDNA
Clone info.	Plasmid clone of human P-glycoprotein cDNA.
Comment	Please refer Fig1. A of Biochem. Biophys. Res. Commun., 162, 224-231 (1989).
Vector backbone	pBluescript KS (+) (Plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Amp^r
Gene/insert name	Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 cDNA
Depositor\|Developer	Ueda, Kazumitsu \|

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human ATP-binding cassette, sub-family B (MDR/TAP), member 1

External Database
Human ATP-binding cassette, sub-family B (MDR/TAP), member 1

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR This material is provided with written consent of Depositor. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Biochem. Biophys. Res. Commun., 162, 224-231 (1989)).

Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Approval form by depositor [Word]

Remarks ((Additional form)) Approval Form(FormD)
Please obtain written approval of the depositor.

提供案内 (日本国内) [open/close]

Terms and conditions set forth by the DEPOSITOR	This material is provided with written consent of Depositor. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Biochem. Biophys. Res. Commun., 162, 224-231 (1989)).
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form by depositor [Word]
Remarks	((Additional form)) Approval Form(FormD) Please obtain written approval of the depositor.

提供条件	寄託者に許可をとること。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Biochem. Biophys. Res. Commun., 162, 224-231 (1989))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 寄託者による提供承諾書 [Word]
備考	【追加提供依頼書】承諾書(FormD) 寄託者の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01372	pMDRA1	DNA solution	Please contact us.

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Remarks, protocol and/or map (gif)	RDB01372.gif

Original reference

original	Kioka, N., P-glycoprotein gene (MDR1) cDNA from human adrenal: normal P-glycoprotein carries Gly185 with an altered pattern of multidrug resistance. Biochem. Biophys. Res. Commun., 162, 224-231 (1989). PMID 2568832.
original	Chen, C.J., Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells. Cell, 47, 381-389 (1986). PMID 2876781.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12371_A5Hsp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB12371_A5Hsa.seq
>01372_12371_A5Hs_M13_-40_A10_01.ab1 1 TAGGCCAGTG AATTGTTATA CGACTCACTA TAGGGCGAAT TGGAGCTCCA CCGCGGTGGC 61 ATTCGAGTAG CGGCTCTTCC AAGCTCAAAG AAGCAGAGGC CGCTGTTCGT TTCCTTTAGG 121 TCTTTCCACT AAAGTCGGAG TATCTTCTTC CAAAATTTCA CGTCTTGGTG GCCGTTCCAA 181 GGAGCGCGAG GTCGGGATGG ATCTTGAAGG GGACCGCAAT GGAGGAGCAA AGAAGAAGAA 241 CTTTTTTAAA CTGAACAATA AAAGTGAAAA AGATAAGAAG GAAAAGAAAC CAACTGTCAG 301 TGTATTTTCA ATGTTTCGCT ATTCAAATTG GCTTGACAAG TTGTATATGG TGGTGGGAAC 361 TTTGGCTGCC ATCATCCATG GGGCTGGACT TCCTCTCATG ATGCTGGTGT TTGGAGAAAT 421 GACAGATATC TTTGCAAATG CAGGAAATTT AGAAGATCTG ATGTCAAACA TCACTAATAG 481 AAGTGATATC AATGATACAG GGTTCTTCAT GAATCTGGAG GAAGACATGA CCAGGTATGC 541 CTATTATTAC AGTGGAATTG GTGCTGGGGT GCTGGTTGCT GCTTACATTC AGGTTTCATT 601 TTGGTGCCTG GCAGCTGGAA GACAAATACA CAAAATTAGA AAACAGTTTT TTCATGCTAT 661 AATGCGACAG GAGATAGGCT GGTTTGATGT GCACGATGTT GGGGAGCTTA ACACCCGACT 721 TACAGATGAT GTCTCCAAGA TTAATGAAGG AATTGGTGAC AAAATTGGAA TGTTCTTTCA 781 GTCAATGGCA ACATTTTTCA CTGGGTTTAT AGTAGGATTT ACACGTGGTT GGAAGCTAAC 841 CCTTGTGATT TTGGCCATCA GTCCTGTTCT TGGACTGTCA GCTGCTGTCT GGGCAAAGAT 901 ACTATCTTCA TTTACTGATA AAGAACTCTT AGCGTATGCA AAAGCTGGAG CAGTAGCTGA 961 AGAGGTCTTG GCAGCAATTA GAACTGTGAT TGCATTTGGG AGGACAAAAG AAAGAACTTG 1021 AAAGGTACAC AAAAATTTAG AAGAGCTAAA GAATTGGGAA TAAAGAAGCT ATACAGCCCA 1081 ATATTTTCTA TAGGTGCTGC TTTCCTGCTG AATCTATGCA ATCTAATGGC TCTGGACATT 1141 CTGGTTATGG GGAACCAACC TTGGGTTGCT //
Primer: Reverse2 Sequence file: RDB12371_A5Hsb.seq
>01372_12371_A5Hs_Reverse2_G09_21.ab1 1 AGGTAACCTT GAAATTTCGG CCAAGCTCGA AATTAACCCT CACTAAAGGG AACAAAAGCT 61 GGTACCGGGC CCCCCCTCGA GGTCGACGGT ATCGATAAGC TTGATATCGA ATTCCTGCAG 121 CCCTTTTTTT TTTTTTTCCA GTCACATGAA AGTTTAGTTT TATTATAGAC ACTTTATGCA 181 AACATTTCAA TACTTTTTGC TACTTCTATA ATCTTTTAGC AAGGCAGTCA GTTACAGTCC 241 AAATGGGAAA ATATAAACAA AATTACACAT TTTATCTTTT AAAATCTACT TTAATTCTGT 301 TATAAAATTT ATAATGCAGT TTAAACTACG ATTTCTCTCC ACTTGATGAT GTCTCTCACT 361 CTGTTCCTTT AATTACGAAG TCTCTGAAGA CTCTGAACTT GACTGAGGAA ATGTTAAACA 421 GATACCTCTT CATAATTCTG TAAGTGTTTG CTTTTAACTT TGAATAAATG TCATATCTAA 481 ACAAATATTA AAAAGTATTT AACATCTCAT ACAGTCAGAG TTCACTGGCG CTTTGTTCCA 541 GCCTGGACAC TGACCATTGA AAAATAGATG CCTTTCTGTG CCAGCAGCTG CTGATGCGTG 601 CCATGCTCCT TGACTCTGCC ATTCTGAAAC ACCACTATTA AGTCTGCATT CTGGATGGTG 661 GACAGGCGGT GAGCAATCAC AATGCAGGTG CGGCCTTCTC TGGCTTTGTC CAGGGCTTCT 721 TGGACAACCT TTTCACTTTC TGTATCCAGA GCTGACGTGG CTTCATCCAA AAGCAAAATA 781 TGAGGCTGTC TAACAAGGGC ACGAGCTATG GCAATGCGTT GTTTCTGGCC ACCAGAGAGC 841 TGAGTTCCTT TGTCTCCTAC TTTAGTGCTA TATTTATTAG GCAGTGACTC GATGAAGGCA 901 TGTATGTTGG CCTCCTTTGC TGCCCTCACA ATCTCTTCCT GTGACACCAC CCCGGCTGTT 961 GTCTCCATAG GCAATGTTCT CAGCAATGCT GCAGTCAAAC AGGATGGGGC TCCCTGGGAC 1021 ACGATGCCCA AGGTGTGCTC GGAGCCACTG AACATTCAAG TCGCTTTATT CTTTTGCATC 1081 AGGCAGCACT TTCCCTTGCC AGGGGTCGTA GAACCGCCTC AAGAGCTGGA ACACTGTGCT 1141 CTCCCACAGC CACTGTCTGA TCCAACCAGA AGTCCAGCGC GTCTG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kioka, N., P-glycoprotein gene (MDR1) cDNA from human adrenal: normal P-glycoprotein carries Gly185 with an altered pattern of multidrug resistance. Biochem. Biophys. Res. Commun., 162, 224-231 (1989). PMID 2568832.
original	Chen, C.J., Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells. Cell, 47, 381-389 (1986). PMID 2876781.
user_report	Teng, Y.N., beta-carotene reverses multidrug resistant cancer cells by selectively modulating human P-glycoprotein function. Phytomedicine 23 (3): 316-323, 2016. PMID 26969385.
user_report	Xiang, Q.F., Cabozantinib reverses multidrug resistance of human hepatoma HepG2/adr cells by modulating the function of P-glycoprotein. Liver Int. 35 (3): 1010-1023 (2015). PMID 24621440.
user_report	Teng, Y.N., Demethoxycurcumin Modulates Human P-Glycoprotein Function via Uncompetitive Inhibition of ATPase Hydrolysis Activity. J. Agric. Food Chem. 63 (3): 847-855 (2015). PMID 25594233.
user_report	Sheu, M.J., The functional influences of common ABCB1 genetic variants on the inhibition of P-glycoprotein by Antrodia cinnamomea extracts. PLoS One, 9 (2):e89622 (2014). PMID 24586917.
user_report	Hung, C.C., Functional impact of ABCB1 variants on interactions between P-glycoprotein and methadone. PLoS One. 2013;8(3):e59419. PMID 23527191.
user_report	Hung, C.C., YC-1, a novel potential anticancer agent, inhibit multidrug-resistant protein via cGMP-dependent pathway. Invest New Drugs 29 (6): 1337-1346 (2011). PMID 20676745.
user_report	Kugawa F., Construction of a model cell line for the assay of MDR1 (multi drug resistance gene-1) substrates/inhibitors using HeLa cells. Pharmazie, 64, 296-300 (2009). PMID 19530439.
user_report	Hung C.C., Functional evaluation of polymorphisms in the human ABCB1 gene and the impact on clinical responses of antiepileptic drugs. Pharmacogenet. Genomics, 18, 390-402 (2008). PMID 18408562.
user_report	Morita N., Human MDR1 polymorphism: G2677T/A and C3435T have no effect on MDR1 transport activities. Biochem. Pharmacol., 65, 1843-1852 (2003). PMID 12781336.
user_report	Fujise H., Transepithelial transport and cellular accumulation of steroid hormones and polychlorobiphenyl in porcine kidney cells expressed with human P-glycoprotein. Chemosphere., 46, 1505-1511 (2002). PMID 12002483.

2022.05.18

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