Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMDRA1 (#RDB01372)

Plasmid clone of human P-glycoprotein cDNA

Alternative name	Human P-glycoprotein cDNA
Clone info.	Plasmid clone of human P-glycoprotein cDNA.
Comment	Please refer Fig1. A of Biochem. Biophys. Res. Commun., 162, 224-231 (1989).
Vector backbone	pBluescript KS (+) (Plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Amp^r
Gene/insert name	Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 cDNA
Depositor|Developer	Ueda, Kazumitsu |

 

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Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form by depositor [Word] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	This material is provided with written consent of Depositor. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Biochem. Biophys. Res. Commun., 162, 224-231 (1989)).
Remarks	((Additional form)) Approval Form(FormD) Please obtain written approval of the depositor.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01372	pMDRA1	DNA solution	Please contact us.

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pMDRA1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01372).

Reference section:

Kioka, N., Tsubota, J., Kakehi, Y., Komano, T., Gottesman, M.M., Pastan, I., Ueda, K., P-glycoprotein gene (MDR1) cDNA from human adrenal: normal P-glycoprotein carries Gly185 with an altered pattern of multidrug resistance. Biochem. Biophys. Res. Commun., 162, 224-231 (1989). PMID 2568832. [link to RRC of NBRP]

Chen, C.J., Chin, J.E., Ueda, K., Clark, D.P., Pastan, I., Gottesman, M.M., Roninson, I.B., Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells. Cell, 47, 381-389 (1986). PMID 2876781. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12371_A5Hsp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB12371_A5Hsa.seq
>01372_12371_A5Hs_M13_-40_A10_01.ab1     1 TAGGCCAGTG AATTGTTATA CGACTCACTA TAGGGCGAAT TGGAGCTCCA CCGCGGTGGC    61 ATTCGAGTAG CGGCTCTTCC AAGCTCAAAG AAGCAGAGGC CGCTGTTCGT TTCCTTTAGG   121 TCTTTCCACT AAAGTCGGAG TATCTTCTTC CAAAATTTCA CGTCTTGGTG GCCGTTCCAA   181 GGAGCGCGAG GTCGGGATGG ATCTTGAAGG GGACCGCAAT GGAGGAGCAA AGAAGAAGAA   241 CTTTTTTAAA CTGAACAATA AAAGTGAAAA AGATAAGAAG GAAAAGAAAC CAACTGTCAG   301 TGTATTTTCA ATGTTTCGCT ATTCAAATTG GCTTGACAAG TTGTATATGG TGGTGGGAAC   361 TTTGGCTGCC ATCATCCATG GGGCTGGACT TCCTCTCATG ATGCTGGTGT TTGGAGAAAT   421 GACAGATATC TTTGCAAATG CAGGAAATTT AGAAGATCTG ATGTCAAACA TCACTAATAG   481 AAGTGATATC AATGATACAG GGTTCTTCAT GAATCTGGAG GAAGACATGA CCAGGTATGC   541 CTATTATTAC AGTGGAATTG GTGCTGGGGT GCTGGTTGCT GCTTACATTC AGGTTTCATT   601 TTGGTGCCTG GCAGCTGGAA GACAAATACA CAAAATTAGA AAACAGTTTT TTCATGCTAT   661 AATGCGACAG GAGATAGGCT GGTTTGATGT GCACGATGTT GGGGAGCTTA ACACCCGACT   721 TACAGATGAT GTCTCCAAGA TTAATGAAGG AATTGGTGAC AAAATTGGAA TGTTCTTTCA   781 GTCAATGGCA ACATTTTTCA CTGGGTTTAT AGTAGGATTT ACACGTGGTT GGAAGCTAAC   841 CCTTGTGATT TTGGCCATCA GTCCTGTTCT TGGACTGTCA GCTGCTGTCT GGGCAAAGAT   901 ACTATCTTCA TTTACTGATA AAGAACTCTT AGCGTATGCA AAAGCTGGAG CAGTAGCTGA   961 AGAGGTCTTG GCAGCAATTA GAACTGTGAT TGCATTTGGG AGGACAAAAG AAAGAACTTG  1021 AAAGGTACAC AAAAATTTAG AAGAGCTAAA GAATTGGGAA TAAAGAAGCT ATACAGCCCA  1081 ATATTTTCTA TAGGTGCTGC TTTCCTGCTG AATCTATGCA ATCTAATGGC TCTGGACATT 1141 CTGGTTATGG GGAACCAACC TTGGGTTGCT //
Primer: Reverse2 Sequence file: RDB12371_A5Hsb.seq
>01372_12371_A5Hs_Reverse2_G09_21.ab1     1 AGGTAACCTT GAAATTTCGG CCAAGCTCGA AATTAACCCT CACTAAAGGG AACAAAAGCT    61 GGTACCGGGC CCCCCCTCGA GGTCGACGGT ATCGATAAGC TTGATATCGA ATTCCTGCAG   121 CCCTTTTTTT TTTTTTTCCA GTCACATGAA AGTTTAGTTT TATTATAGAC ACTTTATGCA   181 AACATTTCAA TACTTTTTGC TACTTCTATA ATCTTTTAGC AAGGCAGTCA GTTACAGTCC   241 AAATGGGAAA ATATAAACAA AATTACACAT TTTATCTTTT AAAATCTACT TTAATTCTGT   301 TATAAAATTT ATAATGCAGT TTAAACTACG ATTTCTCTCC ACTTGATGAT GTCTCTCACT   361 CTGTTCCTTT AATTACGAAG TCTCTGAAGA CTCTGAACTT GACTGAGGAA ATGTTAAACA   421 GATACCTCTT CATAATTCTG TAAGTGTTTG CTTTTAACTT TGAATAAATG TCATATCTAA   481 ACAAATATTA AAAAGTATTT AACATCTCAT ACAGTCAGAG TTCACTGGCG CTTTGTTCCA   541 GCCTGGACAC TGACCATTGA AAAATAGATG CCTTTCTGTG CCAGCAGCTG CTGATGCGTG   601 CCATGCTCCT TGACTCTGCC ATTCTGAAAC ACCACTATTA AGTCTGCATT CTGGATGGTG   661 GACAGGCGGT GAGCAATCAC AATGCAGGTG CGGCCTTCTC TGGCTTTGTC CAGGGCTTCT   721 TGGACAACCT TTTCACTTTC TGTATCCAGA GCTGACGTGG CTTCATCCAA AAGCAAAATA   781 TGAGGCTGTC TAACAAGGGC ACGAGCTATG GCAATGCGTT GTTTCTGGCC ACCAGAGAGC   841 TGAGTTCCTT TGTCTCCTAC TTTAGTGCTA TATTTATTAG GCAGTGACTC GATGAAGGCA   901 TGTATGTTGG CCTCCTTTGC TGCCCTCACA ATCTCTTCCT GTGACACCAC CCCGGCTGTT   961 GTCTCCATAG GCAATGTTCT CAGCAATGCT GCAGTCAAAC AGGATGGGGC TCCCTGGGAC  1021 ACGATGCCCA AGGTGTGCTC GGAGCCACTG AACATTCAAG TCGCTTTATT CTTTTGCATC  1081 AGGCAGCACT TTCCCTTGCC AGGGGTCGTA GAACCGCCTC AAGAGCTGGA ACACTGTGCT 1141 CTCCCACAGC CACTGTCTGA TCCAACCAGA AGTCCAGCGC GTCTG //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

original	Kioka, N., P-glycoprotein gene (MDR1) cDNA from human adrenal: normal P-glycoprotein carries Gly185 with an altered pattern of multidrug resistance. Biochem. Biophys. Res. Commun., 162, 224-231 (1989). PMID 2568832. [link to RRC of NBRP]
original	Chen, C.J., Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells. Cell, 47, 381-389 (1986). PMID 2876781. [link to RRC of NBRP]
user_report	Teng, Y.N., beta-carotene reverses multidrug resistant cancer cells by selectively modulating human P-glycoprotein function. Phytomedicine 23 (3): 316-323, 2016. PMID 26969385. [link to RRC of NBRP]
user_report	Xiang, Q.F., Cabozantinib reverses multidrug resistance of human hepatoma HepG2/adr cells by modulating the function of P-glycoprotein. Liver Int. 35 (3): 1010-1023 (2015). PMID 24621440. [link to RRC of NBRP]
user_report	Teng, Y.N., Demethoxycurcumin Modulates Human P-Glycoprotein Function via Uncompetitive Inhibition of ATPase Hydrolysis Activity. J. Agric. Food Chem. 63 (3): 847-855 (2015). PMID 25594233. [link to RRC of NBRP]
user_report	Sheu, M.J., The functional influences of common ABCB1 genetic variants on the inhibition of P-glycoprotein by Antrodia cinnamomea extracts. PLoS One, 9 (2):e89622 (2014). PMID 24586917. [link to RRC of NBRP]
user_report	Hung, C.C., Functional impact of ABCB1 variants on interactions between P-glycoprotein and methadone. PLoS One. 2013;8(3):e59419. PMID 23527191. [link to RRC of NBRP]
user_report	Hung, C.C., YC-1, a novel potential anticancer agent, inhibit multidrug-resistant protein via cGMP-dependent pathway. Invest New Drugs 29 (6): 1337-1346 (2011). PMID 20676745. [link to RRC of NBRP]
user_report	Kugawa F., Construction of a model cell line for the assay of MDR1 (multi drug resistance gene-1) substrates/inhibitors using HeLa cells. Pharmazie, 64, 296-300 (2009). PMID 19530439. [link to RRC of NBRP]
user_report	Hung C.C., Functional evaluation of polymorphisms in the human ABCB1 gene and the impact on clinical responses of antiepileptic drugs. Pharmacogenet. Genomics, 18, 390-402 (2008). PMID 18408562. [link to RRC of NBRP]
user_report	Morita N., Human MDR1 polymorphism: G2677T/A and C3435T have no effect on MDR1 transport activities. Biochem. Pharmacol., 65, 1843-1852 (2003). PMID 12781336. [link to RRC of NBRP]
user_report	Fujise H., Transepithelial transport and cellular accumulation of steroid hormones and polychlorobiphenyl in porcine kidney cells expressed with human P-glycoprotein. Chemosphere., 46, 1505-1511 (2002). PMID 12002483. [link to RRC of NBRP]

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• human ATP-binding cassette, sub-family B (MDR/TAP), member 1

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External Database
Human ATP-binding cassette, sub-family B (MDR/TAP), member 1

• in KEGG Gene
• in NCBI Gene

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