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pUC WT1 (#RDB01304)

WT1 splice variant, exon5(+), KTS(+) cDNA

Alternative name WT1 splice variant, exon5(+), KTS(+) cDNA
Clone info. WT1 splice variant, exon5(+), KTS(+) cDNA.
Comment Known difference (at least): G to C substitution (synonymous) corresponding to 302nt of NM_000378.5 (cds, 180 to 1688).
Vector backbone pUC119 (Plasmid)
Size of vector backbone 3.2 kb
Selectable markers Amp^r
Gene/insert name Human Wilms tumor 1 cDNA
Depositor|Developer Akiyama, Tetsu |
 
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RDB01304 pUC WT1 DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pUC WT1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01304).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16716_A8Isp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 ()
Sequence file: RDB16716_A8Isa.seq check
>01304_16716_A8Is_1_M13_(-40)_B02_05_ABI24.ab1
    1 AAGTCAGCCA TTCGGGTTCA GGCAGCGCCC ACACCCGGGG GCTCTCCGCA ACCCGACCGC
   61 CTGTCCGCTC CCCCACTTCC CGCCCTCCCT CCCACCTACT CATTCACCCA CCCACCCACC
  121 CAGAGCCGGG ACGGCAGCCC AGGCGCCCGG GCCCCGCCGT CTCCTCGCCG CGATCCTGGA
  181 CTTCCTCTTG CTGCAGGACC CGGCTTCCAC GTGTGTCCCG GAGCCGGCGT CTCAGCACAC
  241 GCTCCGCTCC GGGCCTGGGT GCCTACAGCA GCCAGAGCAG CAGGGAGTCC GGGACCCCGG
  301 CGGCATCTGG GCCAAGTTAG GCGCCGCCGA GGCCAGCGCT GAACGTCTCC AGGGCCGGAG
  361 GAGCCGCGGG GCGTCCGGGT CTGAGCCGCA GCAAATGGGC TCCGACGTGC GGGACCTGAA
  421 CGCGCTGCTG CCCGCCGTCC CCTCCCTGGG TGGCGGCGGC GGCTGTGCCC TGCCTGTGAG
  481 CGGCGCGGCG CAGTGGGCGC CGGTGCTGGA CTTTGCGCCC CCGGGCGCTT CGGCTTACGG
  541 GTCGTTGGGC GGCCCCGCGC CGCCACCGGC TCCGCCGCCA CCCCCGCCGC CGCCGCCTCA
  601 CTCCTTCATC AAACAGGAGC CGAGCTGGGG CGGCGCGGAG CCGCACGAGG AGCAGTGCCT
  661 GAGCGCCTTC ACTGTCCACT TTTCCGGCCA GTTCACTGGC ACAGCCGGAG CCTGTCGCTA
  721 CGGGCCCTTC GGTCCTCCTC CGCCCAGCCA GGCGTCATCC GGGCCAGGCC AGGATGTTTC
  781 CTAACGCGCC CTACCTGCCC AGCTGCCTCG AGAGCCAGCC CCGCTATTCG CAATCAGGGT
  841 TACAGCAACG GTCACCTTCG ACGGGACGCC CAGCTACGGT CACACGCCCT CGCCACCATG
  901 CGGCGCAGTT CCCCCAACCA CCTCATTCAA GCATGAGGAT CCCATGGGCC AACCAAGGGC
  961 TCGCTGGGTG GAGCAGCCAG TACTCGGTGG CCGCCCCCCG GTCTTATGGC GTGCCACACC
 1021 CCCACCGAAC AGCCGTGCAC CGGGCAGCGC AGTGCTTTGC CTGCTGGAGG GACGCCCCCT
 1081 ACAGCAAGGA ACAAATTTAT TACCCAATTG ACATCCCCAG CCTTTGGAAG TGCATGAACC
 1141 TGGAAATCCG AATGAACTTA GGGGAGCCCA CCCTTGGAGA GGGGAAGTTG CCTTGCCGTG
1201 GAGACGCTTC ACCAGACGCT ATGAGGTGTA AAAAAATATG AG
//
Primer: Reverse2 ()
Sequence file: RDB16716_A8Isb.seq check
>01304_16716_A8Is_1_Reverse2_H01_22_ABI24.ab1
    1 TTACAAATGA TTACGCCAGC TTGCATGCCT GCAGGTCGAC TCTAGAGGAT CCCCGGGTAC
   61 CGAGCTCGAA TTCCCCTCCA TTTGTGCAAG GAGGTATGTA CATCTATAAA GACACATATG
  121 ATTACCACTC AAAAGAGTCA AAAACATACA CAAAAAAATC TCAGATCTTC ATAGTTTCTT
  181 AAGAGCAGTG TGCCAGTGTT CACATTGAAT TAACTGAATG GTAAAATTCT TTTAGATTTC
  241 TATACAGAGG TACTGGTTAG TTCTGATTTT TTCTTCAGCT GCTTGAAATG CATGAGCTTT
  301 AAAAGTTGCC TGGCAGAACT ACATCCTGCT TTCCAGGTTA GCAGCCTGGC TGACCTCGGG
  361 AATGTTAGAC AAGATTCACC CCCGATGCCT TGCTCTCTGA TTTATTTCTT GCTGTTGCTG
  421 TTAGTAAATG GAAACCATGA CAAAGTTTAC ATTAGCAGAC ACATACACAT GCCCTGGCCT
  481 ATAAATATTC CATGATAGAA AATGGTACAA TAATTCCATC CCCAGCGAAA ACGAGCATAA
  541 AAAAAGAAGG GAAGGGTCAG GGGGACATGA TCAGCTATGG CTCTTCTTAC AGTAGAAAAT
  601 CCACACCAAA TGGCAATGGG CTTTTAACTA ACCAGACATT GTTAGCTGCT TCTCCAGGGC
  661 CTGTGAGTCA ACTAAAAGTA GGCAGGGCAG AGACCAACTC TTCCAGGCAC ACCTGGTAGT
  721 TTCCAGAAGC ACCGGTATCT TGTCTTGGAA GTTGGATGAA GAAGATCAAC TGAAGTTTCC
  781 TTTTTAGTGA GGAGGAGTGG AGAGTCAGAC TTGAAAGCAG TTCACACACT GTGCTGCCTG
  841 GGACACTGAA CGGTCCCCGA GGGAGACCCC TCAAAGCGCC AGCTGGAGTT TGGTCATGTT
  901 TCTCTGATGC ATGTTGTGAT GGCGGACTAA TTCATCTGAC CGGGCAAACT TTTCTGACAA
  961 CTTGGCCACC GACAGCTGAA GGGCTTTTCA CTTGTTTACC TGTATGAGTC CTGGTGTGGG
 1021 TCTTCAGGTG GTCGAACCGG GAGACTTTCG CTGACAAGTT TACACTGGAT TGTTTCACAC
 1081 TGTATTGTCC TCTTTGGTGT CTTTGAGCTG TCTGGAACCG AGAAACTCGT AAGTCCTGAA
1141 GTCAACTGGA TTGGTTCTCT CACCAGGTGT GTCTTCTCCT GGCT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Gessler, M., Homozygous deletion in Wilms tumours of a zinc-finger gene identified by chromosome jumping. Nature, 343, 774-778 (1990). PMID 2154702.