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pco12 EGFR

Human EGF receptor (EGFR) cDNA

Catalog number RDB01276
Resource name pco12 EGFR
Alternative name Human EGF receptor cDNA
Clone info. Human EGF receptor (EGFR) cDNA
Vector backbone pCO12 (Plasmid)
Size of vector backbone 11.2 kb
Selectable markers Amp^r
Gene/insert name Human EGFR cDNA
Depositor|Developer Yamamoto, Tadashi |
Other clones in our bank

External Database
Human EGFR

          Reference sequence
            

          Distribution information

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          RDB01276 pco12 EGFR DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB01276.gif
          Remarks, protocol and/or map (pdf) RDB01276.pdf

          Original reference

          original Velu, T.J., Retroviruses expressing different levels of the normal epidermal growth factor receptor: biological properties and new bioassay. J. Cell Biochem., 39, 153-166 (1989). PMID 2565908.
          original Velu, T.J., Epidermal-growth-factor-dependent transformation by a human EGF receptor proto-oncogene. Science, 238, 1408-1410 (1987). PMID 3500513.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0034e (English text)
          Featured content Dnaconda's recommendation EXR0034j (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18645_B0Lfp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: lacIpro_R1
          Region: pBR322-BamHI-Ha MuSV (LTR)
          Sequence file: RDB18645_B0Lfa.seq check
          >01276_18645_B0Lf_1_lacIpro_R1_F02_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CGCTCNTGAG CCCGAAGTGG CGAGCCCGAT
             61 CTTCCCCATC GGTGATGTCN GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA
            121 TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCCGGATCTC CCTCTCCTCC AACCACCCTT
            181 GGAACGTCTT CCAGGGCTGC GGGGGAGAAG TCCGAGCTCG TCAGCTCCTT CTCTGGCCCG
            241 CCCAGATAGT CCCCAGATCT GAGCCTATTG ATCGATCGTT CACAGGACAA GACACAACAG
            301 ACAAGACAAA CGCCGGCCGG CTTGCCTCCC GGTGGTGGGT CGGTGGTCCC TGGGCAGGGG
            361 TCTCCCGATC CCGGACGAGC CCCCAAATGA AAGACCCCCA CTAACGGGTA GTCAATCACT
            421 TAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG GATGCAACTG CAAGAGGGTT
            481 TATTGGATAT ACGGGTACCC GGGCGACTCA GTCAATCGGA GGACTGGCGC CCCGAGTGAG
            541 GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG CGCGAACAGA AGCGAGAAGC
            601 GAACTGATTG GTTAGTTTNA ATNANGNACA GGGTCATTTC AGGTCCNTGG GGNACCCTNN
            661 AAACNTCNGA NGNNTCTCTN NNNNTGCTGN NGNNTNNNNN GCNNCTGGGG NNCATCTNNN
          721 CNNGNNCNNN NNNNNGGNNN NNNNNNN
          //
          Primer: Amp_R
          Region: pBR322 (AmpR pro)-HindIII-Ha MuSV
          Sequence file: RDB18645_B0Lfb.seq check
          >01276_18645_B0Lf_1_Amp_R_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNANNNGNN NCATTTATNA GGGTTATTGT CTCATGNAGC GGANACATAT
             61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
            121 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA
            181 CGAGGCCCTT TCGTCTTCAA GAATTAATTC TCATGTTTGA CAGCTTATCA TCGATAAGCT
            241 TTCCTCACCA AACGAGAGAC GGCCTCTACA ATCATCAAGA AGATACTGGA AGAAATCTTC
            301 CTCTTGGAAT GCCCAAGGTA ATCTGGTCAG ACAACGGCCC TACTTTCGTT GCCAAGGTAA
            361 GCCAGGGTGT GGCCAAGTAT TTAGAGGTCG ATTGAAAATT ACATTGTATT TACAGACCTC
            421 AAAGTTCAGG ACAGGTAGGG TAAATAAATA AAACTCTAAA AGAGACCCTG ACCAAATTAA
            481 CCATGGAGAC TGGCACAGAC TGGGTGACAC TCCTTCCCTC TTGCTCTCTT CAGAGCAAGA
            541 AATACCCCTT CCAGATTCNG CCTTACCCCC TTTGAGATCT TATATGGGGC CTCAGCCCCA
            601 CTGACTGTAT TANATGANGT TACTGAACCN ACATGTCATA GTGNCATAGT AATAATGANT
            661 TGTGTGNNNN GNNNAAANAN CTACANGNGN NACNGAAAGA AATCTGNTNN NNNNNNGNAN
          721 NNNNNNANNN CNNNGGNNNC CNNNNNNNNN N
          //
          Primer: ESFR-N_rev1
          Region: insert 5'(hEGFR 5')-SacII-Ha MuSV
          Sequence file: RDB18645_B0Lfc.seq check
          >01276_18645_B0Lf_1_ESFR-N_rev1_C01_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNC CTCCNNGNNG GTCTTTAAGA AGGAAAGANC ATAATTCCTC
             61 TGCACATAGG TAATTTCCAA ATTCCCAAGG ACCACCTCAC AGTTATTGAA CATCCTCTGG
            121 AGGCTGAGAA AATGATCTTC AAAAGTGCCC AACTGCGTGA GCTTGTTACT CGTGCCTTGG
            181 CAAACTTTCT TTTCCTCCAG AGCCCGACTC GCCGGGCAGA GCGCAGCCAG CAGCGCCAGG
            241 AGCGCTGCCC CGGCCGTCCC GGAGGGTCGC ATCGCTGCTC CCCGAAGAGC TCGCTCCGGC
            301 TCTCCCGATC AATACTGGAC GGAGTCAGGG GGCCGTGCGC GGTGGTTGTG GCGTTGGCGG
            361 CGAGGCGGGG ACTCGGGCGG ACGCCGACGA GGTGGCCTGT CGTCCGGTCT GGGCGGCGGC
            421 GGCCGCCGGG GCCATCGACT CTAGCGGATC CGCGGCGGGT GCGGCTCGGG CTGCGGGCAT
            481 AAGCACAGAT AAAACACTAT ATGTTTTTGT TTTGTTTCTC TTTCCTTTTT TTCAGAGCTG
            541 GGGACCGAAC CCAGGGCCTT GCGCTTGCTA GGCAAGAGCT CTATCACTGA NCTAAATCCC
            601 CAACCCCCAA AACACTATGT TTTAAAAATT AACTTTGGCT ATCNACNNAC ACACTGCCNC
            661 TAGAGCGGGG NCTACAAAAN TNNANTTTCN TANTCNCTAA NCNNNNANNN NNNNNANTAA
          721 ANCTNNTNNN NNNANNNAAN NNNAANNNNN TTNNNNNNNN NN
          //
          Primer: pCO12_R
          Region: Ha MuSV-XhoI-insertt 3' (hEGFR 3')
          Sequence file: RDB18645_B0Lfd.seq check
          >01276_18645_B0Lf_1_pCO12_R_E01_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN GNGCCGGGNN GGGCACAAAG GNCATGACTG ACCCTCGAGA
             61 CATGGGCCTG CAGGTCGATG GAAAGATAAA TTTCTGCTCA AAGGGACAAT ATTCTTGCTG
            121 GATGCGTTTC TGTAAATGCT TCACAGTTTG AAGACAAAGG AATGCAACTT CCCAAAATGT
            181 GCCCGAGGTG GAAGTACTTC CTGGCTAGTC GGTGTAAACG TTGCAAAACC AGTCTGTGGG
            241 TCTAAGAGCT AATGCGGGCA TGGCTGTTGG GATGGAGGAC CTGCTGTGGC TTGGTCCTGG
            301 GTATCGAAAG AGTCTGGATT TTTAGGGCTC ATACTATCCT CCGTGGTCAT GCTCCAATAA
            361 ATTCACTGCT TTGTGGCGCG ACCCTTAGGT ATTCTGCATT TTCAGCTGTG GAGCCCTTAA
            421 AGATGCCATT TGGCTTGGCT TCCTTGGGAA AGAAGTCCTG CTGGTAGTCA GGGTTGTCCA
            481 GGCTAATTTG GTGGCTGCCT TTCTGGGCCC AGTGGGCAGG GCTGTCGAAT GTGCTGTTGA
            541 CACAGGTGGG CTGGACAGTG TTGAGATACT CGGGGTTGCC CACTGCAGTG CTGTGGGGGT
            601 CCTGGTAGTG TGGGTCTCTG CTGGGCGCGG GGTTCANANG CTGANTGTGA TANACAGGAN
            661 TCTGCNCAGA NCCNGCGGGN CNTTTGGNAA CGGACTNNTT TANGNNTTNN NNNNCTGGGN
          721 NNNNANGNNG TCNNNNN
          //
          Primer: ESFR-C_F1
          Region: insert 3'-XhoI-Ha MuSV
          Sequence file: RDB18645_B0Lfe.seq check
          >01276_18645_B0Lf_1_ESFR-C_F1_F01_16_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN TCCNGNNNNN NNATTGTCCC TTTGANCAGA AATTTATCTT
             61 TCCNTCGACC TGCAGGCCCA TGTCTCGAGG GTCAGTCATG TCCTTTGTGC CCGTCCCGGC
            121 ACAGGCTCAG GACGAGGAGG TGCCGGATGC AGGGAGGTAG GTACTCCTTA AGGGAAAATC
            181 TAAGCCCAGA GAGACAGCCG GTAACAGCTA GGCTGCCTCT CTGCTCGCCT TTCTGTTTCA
            241 TAGACAAAGC TTGTGCTTAT ATTTACAGTG GCCTTTTTGT CCAAAAAAAG GCCTCCTGGT
            301 TTAGCTGTGT GACTAAATGC TATTTGTCCT CTTCAGTGGA CCTCTCTCCT CTAGATTCCC
            361 TTCTTGTTTT CTCACCATCC CACGTGAGAT GCTTGTTAGT AACTGTTACA GATCTTCTTT
            421 ACCACTGAGG AAAGACAGAA TCCTGCTAAA GGCCAGAAAG AATGTTCCAG ACATGGATGG
            481 AAGGCTCCTG ACTGTTGACT TCAATGCCCC TGAAGGTAGG GAGTGCTCCA GGTCTGCCCC
            541 CAGGCTCCGA GGGTGGGTCT CCTAGGGGCT AGAAAATGCC CCACNNATCT GGCTAAGATA
            601 AGAAAAAGAT ATAAAGAGAA AGTTACNGAA ACTTGAAGGG TAAAGCTAAG TCACTGAAAG
            661 AGNTATTATA AGTTACAGAN AAAATAANTT NANGCNNNNN NNCNNNNNNG NGNNNNAANN
          721 NNNNNNNN
          //
          Primer: HaMuSV-3pri_R2
          Region: Ha MuSV (LTR)
          Sequence file: RDB18645_B0Lff.seq check
          >01276_18645_B0Lf_1_HaMuSV-3pri_R2_E02_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNAGTAAAAC TCTTCGACGA ACAAAGCAAA CAGTTTCATG
             61 ATTTCAAACA CAGTCGTCGG TCTAAGATTT TAAACACAGT CGTCAGTCCA AATTCAAACA
            121 CGAAACAAAA GTCAAAAAGA CACTAACAGA CACAACACGT CCAGAAAACC ACAGTCAGGT
            181 CACAAAGAAG ACAAACAATT CCAACAGTCA AACAAGTAAC AAGCAGACGC GCCGCGCAGC
            241 TTCGGTACCA AACTGAAACC AAAAATTCAG ACGGAGTCAT CAAGGGTGCG GATCCCTCCG
            301 AAAACGGACG GAGGTGCCAC GGATCCGGAT CTCCCTCTCC TCCAACCACC CTTGGAACGT
            361 CTTCCAGGGC TGCGGGGGAG AAGTCCGAGC TCGTCAGCTC CTTCTCTGGC CCGCCCAGAT
            421 AGTCCCCAGA TCTGAGCCTA TTGATCGATC GTTCACAGGA CAAGACACAA CAGACAAGAC
            481 AAACGCCGGC CGGCTTGCCT CCCGGTGGTG GGTCGGTGGT CCCTGGGCAG GGGTCTCCCG
            541 ATCCCGGACG AGCCCCCAAA TGAAAGACCC CCACTAACGG GTAGTCAATC ACTTAGAGGA
            601 GACCCTCCCN AGGAACAGCG AGACCACAAG TCGGANGNNA CTGCNAGANG GTTTATTGNA
            661 NATACGGGTA CCCGGGCGAN TCNGTCNATC NNNNNANTGG NNNCCCNNNN GNGGGGNNGN
          721 GGNNNNNTTN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Velu, T.J., Retroviruses expressing different levels of the normal epidermal growth factor receptor: biological properties and new bioassay. J. Cell Biochem., 39, 153-166 (1989). PMID 2565908.
          original Velu, T.J., Epidermal-growth-factor-dependent transformation by a human EGF receptor proto-oncogene. Science, 238, 1408-1410 (1987). PMID 3500513.
          reference Clark, A.J., Synthesis of epidermal growth factor (EGF) receptor in vitro using SP6 RNA polymerase-transcribed template mRNA. Biochim. Biophys. Acta., 867, 244-251 (1986). PMID 3017432.
          user report Su, J., Transmembrane signaling on a protocell: Creation of receptor-enzyme chimeras for immunodetection of specific antibodies and antigens. Sci. Rep. 9 (1): 18189 (2019). PMID 31796769.
          user report Fukuda, N., Rapid Evaluation of Tyrosine Kinase Activity of Membrane-Integrated Human Epidermal Growth Factor Receptor Using the Yeast G gamma Recruitment System. ACS Synth. Biol. 4 (4): 421-429 (2015). PMID 25006755.
          user report Kojima, K., ESCRT-0 Protein Hepatocyte Growth Factor-regulated Tyrosine Kinase Substrate (Hrs) Is Targeted to Endosomes Independently of Signal-transducing Adaptor Molecule (STAM) and the Complex Formation with STAM Promotes Its Endosomal Dissociation. J. Biol. Chem. 289 (48): 33296-33310 (2014). PMID 25296754.
          user report Hiragun, T., Surface plasmon resonance-biosensor detects the diversity of responses against epidermal growth factor in various carcinoma cell lines. Biosens. Bioelectron., 32 (1): 202-207 (2012). PMID 22204782.
          user report Tanaka, K., Selection and growth regulation of genetically modified cells with hapten-specific antibody/receptor tyrosine kinase chimera. Biotechnol. Progr., 25, 1138-1145 (2009). PMID 19569211.
          user report Dyson M.R., Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression BMC Biotechnol., 4, 1-18 (2004). PMID 15598350.
          user report Niikura T., Insulin-like growth factor I (IGF-I) protects cells from apoptosis by Alzheimer's V642I mutant amyloid precursor protein through IGF-I receptor in an IGF-binding protein-sensitive manner. J. Neurosci., 21, 1902-1910 (2001). PMID 11245675.
          user report Fujino T., Enhanced expression of heparin-binding EGF-like growth factor and its receptor in hypertrophied left ventricle of spontaneously hypertensive rats. Cardiovasc. Res., 38, 365-374 (1998). PMID 9709397.
          user report Nakamura N., An epidermal growth factor receptor/Jak2 tyrosine kinase domain chimera induces tyrosine phosphorylation of Stat5 and transduces a growth signal in hematopoietic cells. J. Biol. Chem., 271, 19483-19488 (1996). PMID 8702638.

          2022.02.09

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