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DQB10301 AMALA-4

DQB1*0301 cDNA, HLA- DQB1, expression, cDNA

Catalog number RDB01240
Resource name DQB10301 AMALA-4
Alternative name DQB1*0301 cDNA
Clone info. Expression vector of human HLA-DQB1 cDNA from EBV-B cells AMALA library, HLA-DQB1. cDNA fragment 23nt to 786nt of DQB1*03*01:22 is cloned. 1.5 kb of the genome sequence after the HLA-DQB1 stop codon in human chromosome 6 is inserted downstream of CDS 3'-ter.
Known differences (at least) : 1 to 22nt of the HLA-DQB1 are replaced to another 45 bases, the following amino acid sequence substitution occurs. MSWKKALRIPGG…GLLH[stop] > [stop]LAPGPVLFSSMVCLRIPGG...GLLH[stop]. Vector information has been corrected pTZ18R from pMAMneo (Nov. 14, 2017).
Vector backbone pTZ18R (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (cell)
Gene/insert name Human HLA-DQB1 cDNA
Depositor Kimura, Akinori | Senju, Satoru |
 
Other clones in our bank human HLA-DQB1 (NCBI Gene 3119) |

Distribution information

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RDB01240 DQB10301 AMALA-4 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01240.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

User reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15308_A7Gsp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2 RDB15308_A7Gsa.seq
>1240_15308_A7Gs_Reverse2_H02_23_ABI24.ab1
    1 AAAACATGAT TACGCCAAGC TCTAATACGA CTCACTATAG GGAAAGCTTC TAGAAGACTC
   61 CAGGGTCTGA CTTCTGAAGA GAAGAAGAAA GAGGAAGAGT GGAAGAGAGG ACACAGAGAG
  121 TCTGGCCTGC GGGTCTCTCC TGGTGTTTTG AGAGTTTCTG GATCAGAACT CGGAGACGAC
  181 AGCACAGGGT TCAGGCAAAG TCTTAGTCGC CAGGCAGTGA GGTCAGGGGT GGGGAAGCCC
  241 AGGGCTGGGG ATTCCCCATC TCCACAGTTT CACTTCTGCA CCTAACCTGG GTCAGGTCCT
  301 TCTGTCCGGA CACTGTTGAC GCGCAGTCAG CTCTTACCCC CATTGGGTGG CGCGATCACC
  361 AAGAACCAAT CAGTGTCGCC GCGGACGCTG GATATAAAGT CCACGCAGCC CGCAGAACTC
  421 AGAAGTCGGC TAGCTGGCCC CTGGTCCTGT CCTGTTCTCC AGCATGGTGT GTCTGAGGAT
  481 CCCCGGAGGC CTTCGGGCAG CAACTGTTAC CTTGATGCTG GCGATGCTGA GCACCCCAGT
  541 GGCTGAGGGC AGAGACTCTC CCGAGGATTT CGTGTACCAG TTTAAGGCCA TGTGCTACTT
  601 CACCAACGGG ACGGAGCGCG TGCGTTATGT GACCAGATAC ATCTATAACC GAGAGGAGTA
  661 CGCACGCTTC GACAGCGACG TGGAGGTGTA CCGGGCGGTG ACGCCGCTGG GGCCGCCTGA
  721 CGCCGAGTAC TGGAACAGCC AGAAGGAAGT CCTGGAGAGG ACCCGGGCGG AGTTGGACAC
  781 GGTGTGCAGA CACAACTACC AGCTGGAGCT CCGCACGACC TTGCAGCGGC GAGTGGAGCC
  841 CACAGTGACC ATCTCCCCAT CCAGGACAGA GGCCCTCAAC CACCACAACC TGCTGGTCTG
  901 CTCAGTGACA GATTTCTATC CAGCCCAGAT CAAAGTCCGG TGGTTTCGGA ATGACCAGGA
  961 GGAGACAACC GGCGTTGTGT CCACCCCCCC TTTATTAGGA ACGGGTGACT GGACCCTTTC
 1021 CAGATCCCTG GATGATGCTG GAAATGACTC CCCAGCATGA GACGTCCTAC CACCTGCCAC
1081 GTGGAGCACC TCAAGTCGTC AGACCCTCAT CAACCGATGG AAGTGGGGCC G
//
Primer: RDB01240#2 RDB15308_A7Gsb.seq
>01240_15308_A7Gs_RDB01240#2_A12_03_ABI24.ab1
    1 GTGTAAGATT CTATCCAGCC CAGATCAAGT CCGGTGGTTT CGGAATGACC AGGAGGAGAC
   61 AACCGGCGTT GTGTCCACCC CCCTTATTAG GAACGGTGAC TGGACCTTCC AGATCCTGGT
  121 GATGCTGGAA ATGACTCCCC AGCATGGAGA CGTCTACACC TGCCACGTGG AGCACCCCAG
  181 CCTCCAGAAC CCCATCACCG TGGAGTGGCG GGCTCAGTCT GAATCTGCCC AGAGCAAGAT
  241 GCTGAGTGGC ATTGGAGGCT TCGTGCTGGG GCTCATCTTC CTCGGGCTGG GCCTTATTAT
  301 CCATCACAGG AGTCAGAAAG GGCTCCTGCA CTGACTCCTG AGACTATTTT AACTGGGATT
  361 GGTTATCACT TTTCTGTAAC GCCTGCTTGT CCCTGCCCAG AATTCCCAGC TGCCTGTGTC
  421 AGCCTGTCCC CCTGAGATCA GAGTCCTACA GTGGCTGTCA CGCAGCCACC AGGTCATCTC
  481 CTTTCATCCC CACCTCGAGG CTGATGGCTG TGACCCTGCT TCCTGCACTT ACCCAGAGCC
  541 TCTGCCTGTG CACGGCCAGC TGCGTCTACT GAGGCCCCAA GGGGTTTCTG TTTCTATTCT
  601 CTCCTCAGAC TGCTCAAGAG AAGCACATGA AAACCATTAC CTGACTTTAG AGCTTTTTTA
  661 CATAATTAAA CATGATCCTG AGTTATCTGT ATTCTCAACT TCCTTAATTG AGCAGAGGCA
  721 GGAAATCACT GCAGAATGAA GGAACATACC TTGAGGTGAC CCAGCCAACC TGTGCCCAGA
  781 AGGAGGGTTG TACCTTGAAA AGACACTGAA AGAATTTGGG GTGCAAAGTC AGGGTGGGCA
  841 GAGGAGGTAG AAGATCAACT CAGTTGTTGC ATCATTCATG GTTCTTTCAT ATTGATGCTC
  901 AGTGCAGTGG CCTGAGAATA TCCCAGCCTC TCTTCTGGTT TGGTGAGTGC TATATAAGTA
  961 AGCATGGTGG AATTGTTTGG GGGCAAGAAT ATTAGTGACC CTTGGTCACT GGTTGTTTTC
 1021 AAAACAATTC TGGCAAGTCA CATCAATCCA GAATTAATTT TTACTTTTAA GAAAAGCATA
 1081 ACCAGCCAAT AAAAGGTGAC TTAATTTTTG GATTTCTAAT GAATAGAAAC CCAATAATAA
 1141 TTCGTTCAAT GGTTGCCAAC AAGCCTATGA GAGTTCCAAG AGCTGCTGAA CATTTCACAG
1201 AAGAAA
//
Primer: f1ori_F2 RDB15308_A7Gsc.seq
>01240_15308_A7Gs_f1ori_F2_A02_01_ABI08.ab1
    1 GTGGGATTAT AAGGGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT
   61 AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTCC ATTCGCCATT
  121 CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT
  181 GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC
  241 ACGACGTTGT AAAACGACGG CCAGTGAATT AATTCGAGCT CTGCCATTTG TCCTGTGGGC
  301 TCCACAAGAA ACAAACTGCC CCTTACACCC CTCCACTGTG AGGAGGCTGT GGAGGCTGAG
  361 GTGCTCCACA TGGCTGGTGT AGACATCTGC ACACTGGAAG TCATTTCCAG GACCACAAGG
  421 ATCTGGAAAA TCCAGTCCTC CTTCCTAATA AGAGCGATGG ACACAATGCT GGCTGTCAGC
  481 ATCCCGTGCA CAGGACAATG TGTTTGAGAG GTGTGCATGT TACCCAGGCT TGGCCAATCA
  541 GAATCTTTCC TAAAGTTGTT AAAACTCTGG TAGACATTCT AGAAACATAT ATTAACAGAG
  601 ACAGACAAAC AGACACACAC ACACACACAC ACACACACAC ACACACAGCA AGAGAGAGAG
  661 ATGAGATAAG ATATGAAGTG ATAAGGAAGA GAAATGCAGA AAAATAGATG CAAAAAGAAA
  721 CACGGAGATA GAAAAAATGC AGATAAACAG TGACAATGGA TTAGAAAAAT AGAAAGAAAG
  781 GCAACAATGT AGTGAAAGAC AACAAGGGGG CACGGAAAAA AATGCAGAGA GACACGCAGA
  841 AAGAACTATA CAGGGACAAA GAGATACAGA GAAAGAGAGG CAGATTCACA GATAGAGATG
  901 TAACAGAAAG AGAGAGGTGA AGATCTCGTT GAGTATCTGG AACTAGTCAC TTCTGAAATC
  961 AACTTGACTC TAGGCTTCCT TTTGCACCAT GAACGAAATA TATTTGGGGT TTCTATCATT
 1021 TTAGAAATCA AAAATAGTAC TTTTATTGCT GGGTTATGCT TTCTAAAAAG TAAAAATTAT
 1081 TCTTGAATTG ATGTGACTTT GCCAGATGTT TGGAACATCA GTGATAGGGT CACTATATCT
1141 GGCCCCAATC AAATTTCACA CCCAGTGTGC TT
//

Please visit Sequencing and PCR primers for primer information.

References

References

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl