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CDMmIL-7 (#RDB01209)

Mouse IL-7 cDNA

Alternative name Mouse IL-7 cDNA
Clone info. Mouse IL-7 cDNA from mouse stroma cells ST2 cDNA library.
Vector backbone pCDM8 (Plasmid)
Size of vector backbone 4,356 bp
Selectable markers SupF
Gene/insert name Mouse IL-7 cDNA
Depositor|Developer Sudo, Tetsuo |
 
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01209 CDMmIL-7 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CDMmIL-7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01209).

Reference section:

Sudo, T., Ito, M., Ogawa, Y., Iizuka, M., Kodama, H., Kunisada, T., Hayashi, S., Ogawa, M., Sakai, K., Nishikawa, S., Interleukin 7 production and function in stromal cell-dependent B cell development. J. Exp. Med., 170, 333-338 (1989). PMID 2787384.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB01209_B1Fap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40-3'UTR (Pr0107)
Region: insert 5'
Sequence file: RDB01209_B1Faa.seq check
>01209_for check_B1Fa_3_SV40-3'UTR_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGTCGCGGCC GCGACCTGCA GGCGCAGAAC
   61 TGGTAGGTAT GGAAGATCCC TCGAGATCCA TTGTGCTCTA AAGCAGGCGT GGCGTGGGTG
  121 AGAGGAGTCA GCTGCCTGAA CTGCCCTGCC CAGCACCGGT TTGCGGCCAC CCGGTGGATG
  181 ACCGGGGTCC TGGGAGTGAT TATGGGTGGT GAGAGCCGGC TCCTGCTGCA GTCCCAGTCA
  241 TCATGACTAC ACCCACCTCC CGCAGACCAT GTTCCATGTT TCTTTTAGAT ATATCTTTGG
  301 AATTCCTCCA CTGATCCTTG TTCTGCTGCC TGTCACATCA TCTGAGTGCN NNNNNNAAGA
361 CAAAGAAGGT AAAGCATATG AGAGTGTACT GATGATCANC ATNNNNNANN NNNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: insert 3'
Sequence file: RDB01209_B1Fab.seq check
>01209_for check_B1Fa_3_CMV_Forward_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG
   61 AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA ATACGACTCA
  121 CTATAGGGAG ACCCAAGCTT CTAGAGATCC CTCGACCTCG AGATCCATTG TGTCTAAAGT
  181 TTTTTTTTAA GCATTTTAAA ATATTATCCT TTATTTTGCC AAAATCAGAA TATTGTTCAA
  241 TATTGGTAGG GAGCCATCCA GACAGCCTTG CAGGTATAGA GTTTATCTTA CACATGCGAA
  301 TGCCTTCAGT TCGCTAGCTA AGGGCTTACC CATTTTACCA GTTCCTCAGG ACAAAGGACT
  361 GACACGTAGG AGATGTGGGA TATCTAAGGA AGTTATCGAA AGTCCATTAA CAAAATTGAG
  421 TTTCTTAAAT TATTACATGT TGCAANNNNN NTGAGAGANN NGNTTTCTGG GCAAATGGTG
481 ATTATGAAAC NNNNNNNNNN
//
Primer: mIL7-M_F1 (Pr1021)
Region: insert mid
Sequence file: RDB01209_B1Fac.seq check
>01209_01209_B1Fa_3_mIL7-M_F1_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNC TNNNNCTGCT TGGCTNANTT GAGCAGGAGG CATCCAGGAA
   61 CTTCTGGAAG GGTTTCAACC TCTCCAAGTA TATGAACCAG TAGATTCTTG GAGGTTGTTA
  121 CTACATGTCC TGTTTATATA CTGCCCTTCA AAATTTTATT CCAACAAGTT TTTATTTCTC
  181 TCAGTAGTCT CTTTAGGAAA CATGCATCAT TCTTTTTCTG TTCCTTTACG TTTTTTTCTT
  241 CCTTACTTGT GCAGTTCACC AGTGTTTGTG TGCCTTGTGA TACTGTTAGT AAGTGGACAT
  301 TGAATTCTTC ACTGATATTC ATTTTAAGAA ATTGCTTCAA CTTGCGAGCA GCACGATTTA
  361 GAAAAGCAGC TTCCTTTGTA TCATCACATA CATGTTTTCT AAAAAAGTTT GGTTCATTAT
  421 TCGGGCAATT ACTATCAGTT CCTGTCATTT TGTCCAATTC ATCGATGCTG ATCATCAGTA
  481 CACTCTCATA TGCTTTACCT TCTTTGTCTT TAATGTGGCA CTCAGATGAT GTGACAGGCA
  541 GCAGAACAAG GATCAGTGGA GGAATTCCAA AGATATATCT AAAAGAAACA TGGAACATGG
  601 TCTGCGGGAN GTGGGNTGTA GTCATGATGA CTGNNANTGC ANCNNNNCCN GCTCTCACNN
  661 NCCNTAATCA CTNCNCNNNA NCCNNGNNCN NNCNNCCGGG NNGGNCCGNA AACCNGNNNN
721 NNGGNNNNGG CNNNNNNNNN N
//
Primer: pAxEF R2 (Pr0059)
Region: SV40 pA
Sequence file: RDB01209_B1Fad.seq check
>01209_01209_B1Fa_3_pAxEF R2_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNTTNNNTTG TGNNTTTGTG ANGCTATTGC TTTATTTGTA ACCATTATAA
   61 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG
  121 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG
  181 ATCTCTAGTC AAGGCACTAT ACATCAAATA TTTTTCCATA ATTTTCTTGT ATAGCAGTGC
  241 AGCTTTTTCC TTTGTGGTGT AAATAGCAAA GCAAGCAAGA GTTCTATTAC TAAACACAGC
  301 ATGACTCAAA AAACTTAGCA ATTCTGAAGG AAAGTCCTTG GGGTCTTCTA CCTTTCTCTT
  361 CTTTTTTGGA GGAGTAGAAT GTTGAGAGTC AGCAGTAGCC TCATCATCAC TAGATGGCAT
  421 TTCTTCTGAG CAAAACAGGT TTTCCTCATT AAAGGCATTC CACCACTGCT CCCATTCATC
  481 AGTTCCATAG GTTGGAATCT AAAATACACA AACAATTAGA ATCAGTAGTT TAACACATTA
  541 TACACTTAAA AATTTTATAT TTACCTTAGA GCTTTAAATC TCTGTAGGTA GTTTGTCNNA
  601 TTATGTCACA CCACAGAAGT AAGGNTCCTT CACNAAGATC CTCTANAGTC GCGGCCGCNN
  661 NCTGCANGCG CANANNTGGT ANNTATGNNA NANCCCTCNN NNNNNCNNTG NGNNNNNANN
721 NNNNNNN
//
Primer: f1ori_R3 (Pr0142)
Region: sup F,CMV en
Sequence file: RDB01209_B1Fae.seq check
>01209_01209_B1Fa_3_f1ori_R3_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGTGCT TTCCTCGTTA GAATCAGAGC GGGANCTAAA
   61 CAGGAGGCCG ATTAAAGGGN TTTTAGACAG GAACGGTACG CCAGCTGGAC CGCGGTCTTT
  121 CGGACTTTTG AAAGTGATGG TGGTGGGGGA AGGATTCGAA CCTTCGAAGT CGATGACGGC
  181 AGATTTAGAG TCTGCTCCCT TTGGCCGCTC GGGAACCCCA CCACAGGTAA TGCTTTTACT
  241 GGCCTGCTCC CTTATCGGGA AGCGGGGCGC ATCATATCAA ATGACGCGCC GCTGTAAAGT
  301 GTTACGTTGA GAAAGCTGCT CCCTGCTTGT GTGTTGGAGG TCGCTGAGTA GTGCGCGAGC
  361 AAAATTTAAG CTACAACAAG GCAAGGCTTG ACCGACAATT GCATGAAGAA TCTGCTTAGG
  421 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA
  481 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG
  541 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GANCGCCCAA CGACCCCCGC
  601 CCATTGACGT CNATAATGAC GTATGTTCCC ATAGTAACGC CNATAGGGAC NTTCCATTGA
  661 CGTCAATNNN TGGAGTATTN NCNGNAANNN GNCNNCTTNG NCNGTACATC AAGTGNNNCA
721 TANGNCNANN NNNNCCCCCN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sudo, T., Interleukin 7 production and function in stromal cell-dependent B cell development. J. Exp. Med., 170, 333-338 (1989). PMID 2787384.
reference Namen, A.E., Stimulation of B-cell progenitors by cloned murine interleukin-7. Nature, 333, 571-573 (1988). PMID 3259677.