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pHHO1 (#RDB01207)

Human heme oxygenase cDNA

Alternative name Human heme oxygenase cDNA
Clone info. Human heme oxygenase cDNA from U937 cDNA library, Human heme oxygenase-1
Vector backbone pcD-X (Plasmid)
Selectable markers Amp^r
Gene/insert name Human Heme oxygenase-1 (HO-1) cDNA
Reference of insert sequence X06985 (DDBJ accession number)
Depositor|Developer Shibahara, Shigeki |
 
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RDB01207 pHHO1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pHHO1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01207).

Reference section:

Yoshida, T., Biro, P., Cohen, T., Müller, R.M., Shibahara, S., Human heme oxygenase cDNA and induction of its mRNA by hemin. Eur. J. Biochem., 171, 457-461 (1988). PMID 3345742. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13950_A6Atp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Amp_R
Sequence file: RDB13950_A6Ata.seq check
>01207_13950_A6At_2_Amp_R_C07_07.ab1
    1 ACCTTTTTTT ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
   61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
  121 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA
  181 CGAGGCCCTT TCGTCTTCAA GAATTCTCAT GTTTGACAGC TTATCATCGA TAAGCTTTAA
  241 ATGTTAATTT GCCCTTGGAC AGGCTGAACT TTGAGCTAGG AGTAGCTATT GACCAGTTTT
  301 TAGTAGTTTT TGAGGATGTA AAGGGCACTG GAGGGGAGTC CAGAGATTTG CCTTCAGGTC
  361 AGGGAATTAA TAACCTGGAC AATTTAAGGG ATTATTTGGA TGGCAGTGTT AAGGTAAACT
  421 TAGAAAAGAA ACACCTAAAT AAAAGAACTC AAATATTTCC CCCTGGAATA GTCACCATGA
  481 ATGAGTACAG TGTGCCTAAA ACACTGCAGG GGGGGGGGGG GTCAACGCCT GCCTCCCCTC
  541 GAGCGTCCTC AGCGCAGCCG CCGCCCGCGG AGCCAGCACG AACGAGCCCA GCACCGGCCG
  601 GATGGAGCGT CCGCAACCCG ACAGCATGCC CCAGGATTTG TCAAAGGCCC TGAAGGAGGC
  661 CACCAAGGAG GTGCACACCC AGGCAGAGAA TGCTGAGTTC ATGAGGAACT TTCAGAAGGG
  721 CCAGGTGACC CGAGACGGCT TCAAGCTGGT GATGGGCCTC CCTGTACCCA CATCTAATGT
  781 GGCCCTGGAG GAGGGAGATT GAGCGCAACA AGGGAGAGCC CAGTCTTCGC CCCTGTCTAC
  841 TTCCCAGAAA AGCTGCACCG CAAGGCTGCC CTGAAGCAGG AACCTGGGCC CTTCTGGTAC
  901 GGGCCCCGCT GGACAGGAAG GTCATCCCCC TAACACACCA GCCATGGCAG CGCCTAATGT
  961 GAAGCGGCTC CCACGAGGTT GGGGCGCACA AGAACCCGAA GCTGCTTGTG GACCCACGCC
 1021 TACACCCGCT ACCTTGAGTT GAACCTGTCT GGGGTCAAGT GCTTCAAAAA AAATTGCCAG
 1081 AAAAGCCCCT GGAACCTGGC CTAGCTTCTG GGCCAGAGGA CCGTGGGCTC TATCCTTATC
1141 AACTCCGTAT TGCCGTCA
//
Primer: HMOX1-M_R
Sequence file: RDB13950_A6Atb.seq check
>01207_13950_A6At_2_HMOX1-M_R_B07_04.ab1
    1 GGGCGCCTGC GAGCCTGGTG CCGTGAGGGG CTCTGGTCCT TGGTGTCATG GGTCAGCAGC
   61 TCCTGCAACT CCTCAAAGAG CTGGATGTTG AGCAGGAACG CAGTCTTGGC CTCTTCTATC
  121 ACCCTCTGCC TGACTGCGGG AGTCATCTCC AGGGAGTTCA TGCGGGAGCG GTAGAGCTGC
  181 TTGAACTTGG TGGCACTGGC AATGTTGGGG AAGGTGAAGA AGGCCAGGCC CTCGCCAGAG
  241 CTGGGCAGGT CCAGGGCTTT CTGGGCAATC TTTTTGAGCA CCTGGCCCCC AGACAGGTCA
  301 CCCAGGTAGC GGGTGTAGGC GTGGGCCACC AGCAGCTCGG GCTCTGTGCG CCCCACCTCG
  361 TGGAGCCGCT TCACATAGCG CTGCATGGCT GGTGTGTAGG GGATGACCTC CTGCCAGCGG
  421 GGCCCGTACC AGAAGGCCAG GTCCTGCTCC AGGGCAGCCT TGCGGTGCAG CTCTTCTGGG
  481 AAGTAGACAG GGGCGAAGAC TGGGCTCTCC TTGTTGCGCT CAATCTCCTC CTCCAGGGCC
  541 ACATAGATGT GGTACAGGGA GGCCATCACC AGCTTGAAGC CGTCTCGGGT CACCTGGCCC
  601 TTCTGAAAGT TCCTCATGAA CTCAGCATTC TCTGCCTGGG TGTGCACCTC CTTGGTGGCC
  661 TCCTTCAGGG CCTCTGACAA ATCCTGGGGC ATGCTGTCGG GTTGCGGACG CTCCATCCGG
  721 CCGGTGCTGG GCTCGTTCGT GCTGGCTCCG CGGGCGGCGG CTGCGCTGAG GACGCTCGAG
  781 GGGAGGCAGG CGTTGACCCC CCCCCCCCCC CTGCAGTGTT TTAGGCACAC TGTACTCATT
  841 CATGGTGACT ATTCCAGGGG GAAATATTTG AGTTCTTTTA TTTAGTGTTT CTTTTCTAAG
  901 TTTACCTTTA ACACTGCCAT CCAAATAATC CCTTTAAATT GTCCAGGTTA TTAAATTCCC
  961 TGACCTGAAG GCAAAATCTC TGGGACTCCC CTCAGTGCCC TTTACATCTC AAAACCTAAC
 1021 TAAAAACTGG TTCATAGCTA CTTCTTAGCT TCAAAGGTCA GCTGGTCGAA GGCAAGTACA
1081 TTAAGCCTAT CGATAAT
//
Primer: HMOX1-M_F
Sequence file: RDB13950_A6Atc.seq check
>01207_13950_A6At_2_HMOX1-M_F_A07_01.ab1
    1 CGTCAGTACA GACAGAGCCC CTCACGGGCA CCAGGGCTTC GCCAGCGGGC CAGCAACAAA
   61 GTGCAAGATT CTGCCCCCGT GGAGACTCCC AGAGGGAAGC CCCCACTCAA CACCCGCTCC
  121 CAGGCTCCGC TTCTCCGATG GGTCCTTACA CTCAGCTTTC TGGTGGCGAC AGTTGCTGTA
  181 GGGCTTTATG CCATGTGAAT GCAGGCATGC TGGCTCCCAG GGCCATGAAC TTTGTCCGGT
  241 GGAAGGCCTT CTTTCTAGAG AGGGAATTCT CTTGGCTGGC TTCCTTACCG TGGGCACTGA
  301 AGGCTTTCAG GGCCTCCAGC CCTCTCACTG TGTCCCTCTC TCTGGAAAGG AGGAAGGAGC
  361 CTATGGCATC TTCCCCAACG AAAAGCACAT CCAGGCAATG GCCTAAACTT CAGAGGGGGC
  421 GAAGGGATCA GCCCTGCCCT TCAGCATCCT CAGTTCCTGC AGCAGAGCCT GGAAGACACC
  481 CTAATGTGGC AGCTGTCTCA AACCTCCAAA AGCCCTGAGT TTCAAGTATC CTTGTTGACA
  541 CGGCCATGAC CACTTTCCCC GTGGGCCATG GCAATTTTTA CACAAACCTG AAAAGATGTT
  601 GTGTCTTGTG TTTTTGTCTT ATTTTTGTTG GAGCCACTCT GTTCCTGGCT CAGCCTCAAA
  661 TGCAGTATTT TTGTTGTGTT CTGTTGTTTT TATAGCAGGG TTGGGGTGGT TTTTGAGCCA
  721 TGCGTGGGTG GGGAGGGAGG TGTTTAACGG CACTGTGGCC TTGGTCTAAC TTTTGTGTGA
781 AATAATAAAC AACATTGTCT G
//
Primer: pGEX-R
Sequence file: RDB13950_A6Atd.seq check
>01207_13950_A6At_2_pGEX-R_F08_17.ab1
    1 TTCTTACGAA CGCGCGAGGC AGCTGGTTCT TTCCGCCTCA GAAGGTACTT TTTTTTTTTT
   61 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TCCAAACAAT GTTGTTTATT ATTTCACACA
  121 AAAGTTAAAC CAAGGCCACA GTGCCGTTAA ACACCTCCCT CCCCACCCAC GCATGGCTCA
  181 AAAACCACCC CAACCCTGCT ATAAAAACAA CAAAACACAA CAAAAATACT GCATTTGAGG
  241 CTGAGCCAGG AACAAAGTGG CTCCAACAAA AATAAGACAA AAACACAAGA CACAACATCT
  301 TTTCAGGTTT GTGTAAAAAT TGCCATGGCC CACGGGGAAA GTGGTCATGG CCGTGTCAAC
  361 AAGGATACTT GAAACTCAGG GCTTTTGGAG GTTTGAAACA GCTGCCACAT TAGGGTGTCT
  421 TCCAGGCTCT GCTGCAGGAA CTGAGGATGC TGAAGGGCAG GGCTGATCCC TTCCCCCCCT
  481 CTGAAGTTTA GGCCATTGCC TGGATGTGCT TTTCGTTGGG GAAAATGCCA TAGGCTCCTT
  541 CCTCCTTTCC AAAAAGAGGG ACACAGTGAG AGGGCTGGAG GCCCTGAAAG CCTTCAGTGC
  601 CCACGGTAAG GAAGCCAGCC AAGAGAATTC CCTCTCTAAA AAGAAGGCCT TCCACCGGAC
  661 AAAGTTCATG GCCCTGGGAG CCAGCATGCC TGCATTCACA TGGCATAAAG CCCTACAGCA
  721 ACTGTCGCCA CCAGAAAGCT GAGTGTAAGG ACCCATCGGA GAAGCGGAGC CTGGGAGCGG
  781 GTGTTGAGTG GGGGCTTCCC TCTGGGAGTC TCCACGGGGG CAGAATCTTG CACTTTGTTG
  841 CTGGCCCGCT GGCGAAGCCC TGGTGCCCGT GAGGGGCTCT GGTCCTTGGG TGTCATGGGT
  901 CAGCAGCTCC TGCAACTCCT CAAAAGAGCT GGATGTTGAG CAGGAACGCA GTCTTGGCCC
  961 TCTTTCTAAT CACCCCCTCT GCCTGGACTG CGGGGAGTCA TCCTCCAAGG GGGAGTTTCA
 1021 TGGCGGGGAA GCCGGGTAGA GCTGCTTGAA CTTGGTGGCA CTGGCCAATG GTTGGGGAAG
1081 GTGAAAGAAA GGACCAGGCT CCTTCCGGTC CAAGAAAGTC T
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yoshida, T., Human heme oxygenase cDNA and induction of its mRNA by hemin. Eur. J. Biochem., 171, 457-461 (1988). PMID 3345742. [link to RRC of NBRP]
user_report Takahashi S., Oxygen tension regulates heme oxygenase-1 gene expression in mammalian cell lines. Cell Biochem. Funct., 16, 183-193 (1998). PMID 9747510. [link to RRC of NBRP]