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pHHO1

Human heme oxygenase cDNA

Catalog number RDB01207
Resource name pHHO1
Alternative name Human heme oxygenase cDNA
Clone info. Human heme oxygenase cDNA from U937 cDNA library, Human heme oxygenase-1
Vector backbone pcD-X (Plasmid)
Selectable markers Amp^r
Gene/insert name Human Heme oxygenase-1 (HO-1) cDNA
Reference of insert sequence X06985 (DDBJ accession number)
Depositor|Developer Shibahara, Shigeki |
Other clones in our bank

External Database
Human Heme oxygenase-1 (HO-1)

          Reference sequence
            

          Distribution information

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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB01207 pHHO1 DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB01207.gif

          Original reference

          original Yoshida, T., Human heme oxygenase cDNA and induction of its mRNA by hemin. Eur. J. Biochem., 171, 457-461 (1988). PMID 3345742.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13950_A6Atp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: Amp_R
          Sequence file: RDB13950_A6Ata.seq check
          >01207_13950_A6At_2_Amp_R_C07_07.ab1
              1 ACCTTTTTTT ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
             61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
            121 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA
            181 CGAGGCCCTT TCGTCTTCAA GAATTCTCAT GTTTGACAGC TTATCATCGA TAAGCTTTAA
            241 ATGTTAATTT GCCCTTGGAC AGGCTGAACT TTGAGCTAGG AGTAGCTATT GACCAGTTTT
            301 TAGTAGTTTT TGAGGATGTA AAGGGCACTG GAGGGGAGTC CAGAGATTTG CCTTCAGGTC
            361 AGGGAATTAA TAACCTGGAC AATTTAAGGG ATTATTTGGA TGGCAGTGTT AAGGTAAACT
            421 TAGAAAAGAA ACACCTAAAT AAAAGAACTC AAATATTTCC CCCTGGAATA GTCACCATGA
            481 ATGAGTACAG TGTGCCTAAA ACACTGCAGG GGGGGGGGGG GTCAACGCCT GCCTCCCCTC
            541 GAGCGTCCTC AGCGCAGCCG CCGCCCGCGG AGCCAGCACG AACGAGCCCA GCACCGGCCG
            601 GATGGAGCGT CCGCAACCCG ACAGCATGCC CCAGGATTTG TCAAAGGCCC TGAAGGAGGC
            661 CACCAAGGAG GTGCACACCC AGGCAGAGAA TGCTGAGTTC ATGAGGAACT TTCAGAAGGG
            721 CCAGGTGACC CGAGACGGCT TCAAGCTGGT GATGGGCCTC CCTGTACCCA CATCTAATGT
            781 GGCCCTGGAG GAGGGAGATT GAGCGCAACA AGGGAGAGCC CAGTCTTCGC CCCTGTCTAC
            841 TTCCCAGAAA AGCTGCACCG CAAGGCTGCC CTGAAGCAGG AACCTGGGCC CTTCTGGTAC
            901 GGGCCCCGCT GGACAGGAAG GTCATCCCCC TAACACACCA GCCATGGCAG CGCCTAATGT
            961 GAAGCGGCTC CCACGAGGTT GGGGCGCACA AGAACCCGAA GCTGCTTGTG GACCCACGCC
           1021 TACACCCGCT ACCTTGAGTT GAACCTGTCT GGGGTCAAGT GCTTCAAAAA AAATTGCCAG
           1081 AAAAGCCCCT GGAACCTGGC CTAGCTTCTG GGCCAGAGGA CCGTGGGCTC TATCCTTATC
          1141 AACTCCGTAT TGCCGTCA
          //
          Primer: HMOX1-M_R
          Sequence file: RDB13950_A6Atb.seq check
          >01207_13950_A6At_2_HMOX1-M_R_B07_04.ab1
              1 GGGCGCCTGC GAGCCTGGTG CCGTGAGGGG CTCTGGTCCT TGGTGTCATG GGTCAGCAGC
             61 TCCTGCAACT CCTCAAAGAG CTGGATGTTG AGCAGGAACG CAGTCTTGGC CTCTTCTATC
            121 ACCCTCTGCC TGACTGCGGG AGTCATCTCC AGGGAGTTCA TGCGGGAGCG GTAGAGCTGC
            181 TTGAACTTGG TGGCACTGGC AATGTTGGGG AAGGTGAAGA AGGCCAGGCC CTCGCCAGAG
            241 CTGGGCAGGT CCAGGGCTTT CTGGGCAATC TTTTTGAGCA CCTGGCCCCC AGACAGGTCA
            301 CCCAGGTAGC GGGTGTAGGC GTGGGCCACC AGCAGCTCGG GCTCTGTGCG CCCCACCTCG
            361 TGGAGCCGCT TCACATAGCG CTGCATGGCT GGTGTGTAGG GGATGACCTC CTGCCAGCGG
            421 GGCCCGTACC AGAAGGCCAG GTCCTGCTCC AGGGCAGCCT TGCGGTGCAG CTCTTCTGGG
            481 AAGTAGACAG GGGCGAAGAC TGGGCTCTCC TTGTTGCGCT CAATCTCCTC CTCCAGGGCC
            541 ACATAGATGT GGTACAGGGA GGCCATCACC AGCTTGAAGC CGTCTCGGGT CACCTGGCCC
            601 TTCTGAAAGT TCCTCATGAA CTCAGCATTC TCTGCCTGGG TGTGCACCTC CTTGGTGGCC
            661 TCCTTCAGGG CCTCTGACAA ATCCTGGGGC ATGCTGTCGG GTTGCGGACG CTCCATCCGG
            721 CCGGTGCTGG GCTCGTTCGT GCTGGCTCCG CGGGCGGCGG CTGCGCTGAG GACGCTCGAG
            781 GGGAGGCAGG CGTTGACCCC CCCCCCCCCC CTGCAGTGTT TTAGGCACAC TGTACTCATT
            841 CATGGTGACT ATTCCAGGGG GAAATATTTG AGTTCTTTTA TTTAGTGTTT CTTTTCTAAG
            901 TTTACCTTTA ACACTGCCAT CCAAATAATC CCTTTAAATT GTCCAGGTTA TTAAATTCCC
            961 TGACCTGAAG GCAAAATCTC TGGGACTCCC CTCAGTGCCC TTTACATCTC AAAACCTAAC
           1021 TAAAAACTGG TTCATAGCTA CTTCTTAGCT TCAAAGGTCA GCTGGTCGAA GGCAAGTACA
          1081 TTAAGCCTAT CGATAAT
          //
          Primer: HMOX1-M_F
          Sequence file: RDB13950_A6Atc.seq check
          >01207_13950_A6At_2_HMOX1-M_F_A07_01.ab1
              1 CGTCAGTACA GACAGAGCCC CTCACGGGCA CCAGGGCTTC GCCAGCGGGC CAGCAACAAA
             61 GTGCAAGATT CTGCCCCCGT GGAGACTCCC AGAGGGAAGC CCCCACTCAA CACCCGCTCC
            121 CAGGCTCCGC TTCTCCGATG GGTCCTTACA CTCAGCTTTC TGGTGGCGAC AGTTGCTGTA
            181 GGGCTTTATG CCATGTGAAT GCAGGCATGC TGGCTCCCAG GGCCATGAAC TTTGTCCGGT
            241 GGAAGGCCTT CTTTCTAGAG AGGGAATTCT CTTGGCTGGC TTCCTTACCG TGGGCACTGA
            301 AGGCTTTCAG GGCCTCCAGC CCTCTCACTG TGTCCCTCTC TCTGGAAAGG AGGAAGGAGC
            361 CTATGGCATC TTCCCCAACG AAAAGCACAT CCAGGCAATG GCCTAAACTT CAGAGGGGGC
            421 GAAGGGATCA GCCCTGCCCT TCAGCATCCT CAGTTCCTGC AGCAGAGCCT GGAAGACACC
            481 CTAATGTGGC AGCTGTCTCA AACCTCCAAA AGCCCTGAGT TTCAAGTATC CTTGTTGACA
            541 CGGCCATGAC CACTTTCCCC GTGGGCCATG GCAATTTTTA CACAAACCTG AAAAGATGTT
            601 GTGTCTTGTG TTTTTGTCTT ATTTTTGTTG GAGCCACTCT GTTCCTGGCT CAGCCTCAAA
            661 TGCAGTATTT TTGTTGTGTT CTGTTGTTTT TATAGCAGGG TTGGGGTGGT TTTTGAGCCA
            721 TGCGTGGGTG GGGAGGGAGG TGTTTAACGG CACTGTGGCC TTGGTCTAAC TTTTGTGTGA
          781 AATAATAAAC AACATTGTCT G
          //
          Primer: pGEX-R
          Sequence file: RDB13950_A6Atd.seq check
          >01207_13950_A6At_2_pGEX-R_F08_17.ab1
              1 TTCTTACGAA CGCGCGAGGC AGCTGGTTCT TTCCGCCTCA GAAGGTACTT TTTTTTTTTT
             61 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TCCAAACAAT GTTGTTTATT ATTTCACACA
            121 AAAGTTAAAC CAAGGCCACA GTGCCGTTAA ACACCTCCCT CCCCACCCAC GCATGGCTCA
            181 AAAACCACCC CAACCCTGCT ATAAAAACAA CAAAACACAA CAAAAATACT GCATTTGAGG
            241 CTGAGCCAGG AACAAAGTGG CTCCAACAAA AATAAGACAA AAACACAAGA CACAACATCT
            301 TTTCAGGTTT GTGTAAAAAT TGCCATGGCC CACGGGGAAA GTGGTCATGG CCGTGTCAAC
            361 AAGGATACTT GAAACTCAGG GCTTTTGGAG GTTTGAAACA GCTGCCACAT TAGGGTGTCT
            421 TCCAGGCTCT GCTGCAGGAA CTGAGGATGC TGAAGGGCAG GGCTGATCCC TTCCCCCCCT
            481 CTGAAGTTTA GGCCATTGCC TGGATGTGCT TTTCGTTGGG GAAAATGCCA TAGGCTCCTT
            541 CCTCCTTTCC AAAAAGAGGG ACACAGTGAG AGGGCTGGAG GCCCTGAAAG CCTTCAGTGC
            601 CCACGGTAAG GAAGCCAGCC AAGAGAATTC CCTCTCTAAA AAGAAGGCCT TCCACCGGAC
            661 AAAGTTCATG GCCCTGGGAG CCAGCATGCC TGCATTCACA TGGCATAAAG CCCTACAGCA
            721 ACTGTCGCCA CCAGAAAGCT GAGTGTAAGG ACCCATCGGA GAAGCGGAGC CTGGGAGCGG
            781 GTGTTGAGTG GGGGCTTCCC TCTGGGAGTC TCCACGGGGG CAGAATCTTG CACTTTGTTG
            841 CTGGCCCGCT GGCGAAGCCC TGGTGCCCGT GAGGGGCTCT GGTCCTTGGG TGTCATGGGT
            901 CAGCAGCTCC TGCAACTCCT CAAAAGAGCT GGATGTTGAG CAGGAACGCA GTCTTGGCCC
            961 TCTTTCTAAT CACCCCCTCT GCCTGGACTG CGGGGAGTCA TCCTCCAAGG GGGAGTTTCA
           1021 TGGCGGGGAA GCCGGGTAGA GCTGCTTGAA CTTGGTGGCA CTGGCCAATG GTTGGGGAAG
          1081 GTGAAAGAAA GGACCAGGCT CCTTCCGGTC CAAGAAAGTC T
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yoshida, T., Human heme oxygenase cDNA and induction of its mRNA by hemin. Eur. J. Biochem., 171, 457-461 (1988). PMID 3345742.
          user report Takahashi S., Oxygen tension regulates heme oxygenase-1 gene expression in mammalian cell lines. Cell Biochem. Funct., 16, 183-193 (1998). PMID 9747510.

          2022.02.09

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