Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSV2 proto-ret (#RDB01163)

Human protooncogene ret cDNA.

Alternative name Human protooncogene ret cDNA
Clone info. Human ret proto-oncogene cDNA from Human mononuclear leukocyte cell line THP-1. For the construction, refer Fig.5b of Takahashi, M., Oncogene 3, 571-578, 1998.
Vector backbone pSV2-neo (Plasmid)
Size of vector backbone 5,729 bp
Selectable markers Amp^r
Gene/insert name Human ret cDNA
Reference of insert sequence X12949 (DDBJ accession number)
Depositor|Developer Takahashi, Masahide |
 
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01163 pSV2 proto-ret DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pSV2 proto-ret was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01163).

Reference section:

Takahashi, M., Buma, Y., Iwamoto, T., Inaguma, Y., Ikeda, H., Hiai, H., Cloning and expression of the ret proto-oncogene encoding a tyrosine kinase with two potential transmembrane domains. Oncogene, 3, 571-578 (1988). PMID 3078962. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15115_A7Ffp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pro_F_V2
Sequence file: RDB15115_A7Ffa.seq check
>01163_15115_A7Ff_SV40pro_F_V2_F11_06_ABI08.ab1
    1 GTCCAAAGGT TTTTTTTGGG AGGGCCTAGG CTTTTGCAAA AAGGCTTGCC CCGGAACGTG
   61 CGTCGCGCCC CCAGTGTCCG CTCGCGTCCG CCGCGCCCCG GGCGGGGATG GGGCGGCCAG
  121 ACTGAGCGCC GCACCCGCCA TCCAGACCCG CCGGCCCTAG CCGCAGTCCC TCCAGCCGTG
  181 GCCCCAGCGC GCACGGGCGA TGGCGAAGGC GACGTCCGGT GCCGCGGGGC TGCGTCTGCT
  241 GTTGCTGCTG CTGCTGCCGC TGCTAGGCAA AGTGGCATTG GGCCTCTACT TCTCGAGGGA
  301 TGCTTACTGG GAGAAGCTGT ATGTGGACCA GGCGGCCGGC ACGCCCTTGC TGTACGTCCA
  361 TGCCCTGCGG GACGCCCCTG AGGAGGTGCC CAGCTTCCGC CTGGGCCAGC ATCTCTACGG
  421 CACGTACCGC ACACGGCTGC ATGAGAACAA CTGGATCTGC ATCCAGGAGG ACACCGGCCT
  481 CCTCTACCTT AACCGGAGCC TGGACCATAG CTCCTGGGAG AAGCTCAGTG TCCGCAACCG
  541 CGGCTTTCCC CTGCTCACCG TCTACCTCAA GGTCTTCCTG TCACCCACAT CCCTTCGTGA
  601 GGGCGAGTGC CAGTGGCCAG GCTGTGCCCG CGTATACTTC TCCTTCTTCA ACACCTCCTT
  661 TCCAGCCTGC AGCTCCCTCA AGCCCCGGGA GCTCTGCTTC CCAGAGACAA GGCCCTCCTT
  721 CCGCATTCGG GAGAACCGAC CCCCAGGCAC CTTCCACCAG TTCCGCCTGC TGCCTGTGCA
  781 GTTCTTGTGC CCCAACATCA GCGTGGCCTA CAGGCTCCTG GAGGGTGAGG GTCTGCCCTT
  841 CCGCTGCGCC CCGGGACAGC CTGGAGGTGA GCACGCGCTG GGCCCTGGAC CGCGAGCAGC
  901 GGGAGAAGTA CGAGCTGGTG GGCCGTGTGC ACCGTGCACG CCGGCGCGCG CGAGGAGGGT
  961 GGTGATGGTG CCCTTCCCGG TGACCGTGTA CGACGAGGAC GACTCGGGCG CCCACCTTTC
 1021 CCCGGCGGGG CGTCGACACC GCCAACGCCC GTGGTGGAGT TCCAAGCGGA AAGGAGGTGG
 1081 CTTTGTCCCG CGCGTGGCTG TGGGTCTTAC CCAGTTGTTC TGTCTTCGGG CCCGCAGTTC
 1141 GTTTTCTCCG AACAACCCGT GTGATGGCTC ACGCTTGCGT GTCTTTTCGA TGTCGAACCG
 1201 TGGTATCTTG CCTATCAGGG AAGATCCGTG AGTGTAAGAC GCGGTTCACA CTCCAAATAG
//
Primer: RDB01163#1
Sequence file: RDB15115_A7Ffb.seq check
>01163_15115_A7Ff_RDB01163#1_A01_01_ABI08.ab1
    1 ACCAAAAACC GGAAGTCCTG GCCACCTGGG AACTGAACAC GGCTCGGCCA ATGTGACGTT
   61 CACAAAGAAA GGGCCGGTAG ACTTTCCATT CTCAGCATGT TTCTTCCCCT TGTGAGTCCA
  121 TTACCTTTCA CAAAGAAATG TTAACTATCA AACGTGTCCA TTAATTTTGC CGCTGAGGGT
  181 GAAAGCATCC AGTTAGCATA TACACTATCA TTTGGATATC TTGGAAACCC AGTGTTAGTG
  241 CCATCAGCTC TCGTGAGTGG TACAGGACTC TCTCCAGGCC AGTTCGGGTC TGACATGCCA
  301 TAGAGTTTGT TTTCAATCCA TGTGGAAGGG AGGGCTCGAG GGAGGGGGGC ATTATTACAG
  361 TCCACCAGCG GTGTCTCCTC CTCTGAGAGG CCGTCGTCAT AAATCAGGGA GTCAGATGGA
  421 GTGGACGCCG CAAGGTCCAA GTAGTCTCTC CTCTTAACCA TCATCTTCTC CAGGTCTTTG
  481 CTGATGTCCG CAAACACCGG CCTTTTGTCC GGCTCCTGCT TCCAGCATTG CAGCATCAGG
  541 CGGTACATCT CCTCGCTGCA GTTGTCTGGC CTCTCCATCC GGTGGCCGGT CTTCAGAAGG
  601 TTGAAGAGCC GCTCAGGAGG AATCCCAGGA TAGGGGTTTC CCCCTAGGGT CACGATCTCC
  661 CACAGCAGGA CACCAAAAGA CCATACATCA CTTTGCGTGG TGTAGATATG ATCAAAAAGG
  721 GATTCAATTG CCATCCATTT AACTGGGAAT CCGACCCTGG CTCCTCTTCA CGTAGGAATC
  781 CTCTTCATAA ACATCTCGGG ACAAGCCGAA ATCCGAAATC TTCATCTTCC GCCCCTCAGC
  841 TACCAGGATG TTTCTGGCTG CCAAAGTCCC GATGAACGAG CTTCATCTCG GCCAGATACT
  901 GCATCCCCTG TGAGATCTGC CAGGCAAATG AGATGAGGTC GCCCATGGTG AGGGCCCGCT
  961 CATCCGGGGT GGTCCAGGGA GCTGGAGTTG CGGGCTGCCT CCACTGCCCA GGTAGCCAGG
 1021 CCCCACTTTG CGGCTCTCGC CGAGGAGCCC CGGCAGGGAG CCGTAATTGG GCGTACTTCC
 1081 CACGATGAAG AAGGAGCGGG TCAGTCCTGG CTGCAAGGCC CCATACATTG GATTGACATG
 1141 TGGGTTGTGG ACTGCCTTCC AGAACGGTTG GAAACCCTCT TGACACGGCA CAGGTTCTCT
1201 CGCAGAAAGC CCTTCA
//
Primer: SV40NLS_R
Sequence file: RDB15115_A7Ffc.seq check
>01163_15115_A7Ff_SV40NLS_R_B01_02_ABI08.ab1
    1 GAGAGAGGGG TAGCAGTAGC CTCATCATCA CTAGATGGCA TTTCTTCTGA GCAAAACAGG
   61 TTTTCCTCAT TAAAGGCATT CCACCACTGC TCCCATTCAT CAGTTCCATA GGTTGGAATC
  121 TAAAATACAC AAACAATTAG AATCAGTAGT TTAACACATT ATACACTTAA AAATTTTATA
  181 TTTACCTTAG AGCTTTAAAT CTCTGTAGGT AGTTTGTCCA ATTATGTCAC ACCACAGAAG
  241 TAAGGTTCCT TCACAAAGAT CCGGACCAAA GCGGCCATCG TGCCTCCCCA CTCCTGCAGT
  301 TCGGGGGCAT GGATGCGCGG ATAGCCGCTG CTGGTTTCCT GGATGCCGAC GGATTTGCAC
  361 TGCCGGTAGA ACTCCGCGAG GTCGTCCAGC CTCAGGCAGC AGCTGAACCA ACTCGCGAGG
  421 GGATCGAGCC CCAAGCTTAT GAGTCATGGT CAGAATCTAA GAAACAGAAA CATCTTGTAG
  481 CAGCATTAAA TTACAAACAC AGGAAGAGCT GATCCATGGG CCTTTCACAC AGGCTCTATA
  541 AGAGCCAAAC ACCATCTTTT TCACATCAGA ATTGTAATCA GAGGTGTAGC AGTCCTTGGT
  601 CATTGTCATT GCTACTTCTA ACACAGCTAG CTAGCCTGTC CCTTTATGAG TAAGTTGATA
  661 AGATTCAAAT TAAAGTGAGT ACAAGCCCTA CCTGCTCCTA ACAGGTAGGA TGAGCCACTG
  721 CTTTCGCCTC TACTTGGCTG TGGGGTGTGC TGCAGGCTGG TGGCAGGTGG GATTCACAGC
  781 AGCAAAGCCT CTGCTGTCAG CAATTCTGGG ACTCCAACAG CCCCCTTTCT TCATACTGTT
  841 CTCATCAGGG GTAGTGGCTG CTCAGTACCG AACACGTGAG GATGATGTGG TGGGCCCGGG
  901 GGACAAATGT GTCTTGTGAG CCCTCTTCCC TGATACCACC ATCCACATAG TAAAGATGGC
  961 ATGGAATCTC TCCATTGTGC TAAATCTAGG AATCATCTTA ATCATGATGA AAACCAAATG
 1021 TCTTCCTAAG GAAAGGTTGC ATGATTAAAA AAAAAATCAT TCTCAGATCT CGAAATGCTG
 1081 AAGCCCAGGC CATCCTCGGG GACCCTCATC CCCTCCAGTC TTGAGGGCAT GGAACACTAC
1141 CCCAGGTCCC CCCCCGGCAA GTCCTCCTCC AATTTCTTGA ATATAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Takahashi, M., Cloning and expression of the ret proto-oncogene encoding a tyrosine kinase with two potential transmembrane domains. Oncogene, 3, 571-578 (1988). PMID 3078962. [link to RRC of NBRP]
reference Takahashi, M., Isolation of ret proto-oncogene cDNA with an amino-terminal signal sequence. Oncogene, 4, 805-806 (1989). PMID 2660074.
reference Ishizaka, Y., Human ret proto-oncogene mapped to chromosome 10q11.2. Oncogene, 4, 1519-1521 (1989). PMID 2687772.
user_report Nakata T., Differential expression of multiple isoforms of the ELKS mRNAs involved in a papillary thyroid carcinoma. Gene. Chromosome. Canc., 35, 30-37 (2002). PMID 12203787. [link to RRC of NBRP]