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pSV2 proto-ret

Human protooncogene ret cDNA.

Catalog number RDB01163
Resource name pSV2 proto-ret
Alternative name Human protooncogene ret cDNA
Clone info. Human ret proto-oncogene cDNA from Human mononuclear leukocyte cell line THP-1. For the construction, refer Fig.5b of Takahashi, M., Oncogene 3, 571-578, 1998.
Vector backbone pSV2-neo (Plasmid)
Size of vector backbone 5,729 bp
Selectable markers Amp^r
Gene/insert name Human ret cDNA
Reference of insert sequence X12949 (DDBJ accession number)
Depositor|Developer Takahashi, Masahide |
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External Database
Human ret

          Reference sequence
            M57464

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
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          RDB01163 pSV2 proto-ret DNA solution Please contact us.

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          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB01163.gif

          Original reference

          original Takahashi, M., Cloning and expression of the ret proto-oncogene encoding a tyrosine kinase with two potential transmembrane domains. Oncogene, 3, 571-578 (1988). PMID 3078962.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15115_A7Ffp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: SV40pro_F_V2
          Sequence file: RDB15115_A7Ffa.seq check
          >01163_15115_A7Ff_SV40pro_F_V2_F11_06_ABI08.ab1
              1 GTCCAAAGGT TTTTTTTGGG AGGGCCTAGG CTTTTGCAAA AAGGCTTGCC CCGGAACGTG
             61 CGTCGCGCCC CCAGTGTCCG CTCGCGTCCG CCGCGCCCCG GGCGGGGATG GGGCGGCCAG
            121 ACTGAGCGCC GCACCCGCCA TCCAGACCCG CCGGCCCTAG CCGCAGTCCC TCCAGCCGTG
            181 GCCCCAGCGC GCACGGGCGA TGGCGAAGGC GACGTCCGGT GCCGCGGGGC TGCGTCTGCT
            241 GTTGCTGCTG CTGCTGCCGC TGCTAGGCAA AGTGGCATTG GGCCTCTACT TCTCGAGGGA
            301 TGCTTACTGG GAGAAGCTGT ATGTGGACCA GGCGGCCGGC ACGCCCTTGC TGTACGTCCA
            361 TGCCCTGCGG GACGCCCCTG AGGAGGTGCC CAGCTTCCGC CTGGGCCAGC ATCTCTACGG
            421 CACGTACCGC ACACGGCTGC ATGAGAACAA CTGGATCTGC ATCCAGGAGG ACACCGGCCT
            481 CCTCTACCTT AACCGGAGCC TGGACCATAG CTCCTGGGAG AAGCTCAGTG TCCGCAACCG
            541 CGGCTTTCCC CTGCTCACCG TCTACCTCAA GGTCTTCCTG TCACCCACAT CCCTTCGTGA
            601 GGGCGAGTGC CAGTGGCCAG GCTGTGCCCG CGTATACTTC TCCTTCTTCA ACACCTCCTT
            661 TCCAGCCTGC AGCTCCCTCA AGCCCCGGGA GCTCTGCTTC CCAGAGACAA GGCCCTCCTT
            721 CCGCATTCGG GAGAACCGAC CCCCAGGCAC CTTCCACCAG TTCCGCCTGC TGCCTGTGCA
            781 GTTCTTGTGC CCCAACATCA GCGTGGCCTA CAGGCTCCTG GAGGGTGAGG GTCTGCCCTT
            841 CCGCTGCGCC CCGGGACAGC CTGGAGGTGA GCACGCGCTG GGCCCTGGAC CGCGAGCAGC
            901 GGGAGAAGTA CGAGCTGGTG GGCCGTGTGC ACCGTGCACG CCGGCGCGCG CGAGGAGGGT
            961 GGTGATGGTG CCCTTCCCGG TGACCGTGTA CGACGAGGAC GACTCGGGCG CCCACCTTTC
           1021 CCCGGCGGGG CGTCGACACC GCCAACGCCC GTGGTGGAGT TCCAAGCGGA AAGGAGGTGG
           1081 CTTTGTCCCG CGCGTGGCTG TGGGTCTTAC CCAGTTGTTC TGTCTTCGGG CCCGCAGTTC
           1141 GTTTTCTCCG AACAACCCGT GTGATGGCTC ACGCTTGCGT GTCTTTTCGA TGTCGAACCG
           1201 TGGTATCTTG CCTATCAGGG AAGATCCGTG AGTGTAAGAC GCGGTTCACA CTCCAAATAG
          //
          Primer: RDB01163#1
          Sequence file: RDB15115_A7Ffb.seq check
          >01163_15115_A7Ff_RDB01163#1_A01_01_ABI08.ab1
              1 ACCAAAAACC GGAAGTCCTG GCCACCTGGG AACTGAACAC GGCTCGGCCA ATGTGACGTT
             61 CACAAAGAAA GGGCCGGTAG ACTTTCCATT CTCAGCATGT TTCTTCCCCT TGTGAGTCCA
            121 TTACCTTTCA CAAAGAAATG TTAACTATCA AACGTGTCCA TTAATTTTGC CGCTGAGGGT
            181 GAAAGCATCC AGTTAGCATA TACACTATCA TTTGGATATC TTGGAAACCC AGTGTTAGTG
            241 CCATCAGCTC TCGTGAGTGG TACAGGACTC TCTCCAGGCC AGTTCGGGTC TGACATGCCA
            301 TAGAGTTTGT TTTCAATCCA TGTGGAAGGG AGGGCTCGAG GGAGGGGGGC ATTATTACAG
            361 TCCACCAGCG GTGTCTCCTC CTCTGAGAGG CCGTCGTCAT AAATCAGGGA GTCAGATGGA
            421 GTGGACGCCG CAAGGTCCAA GTAGTCTCTC CTCTTAACCA TCATCTTCTC CAGGTCTTTG
            481 CTGATGTCCG CAAACACCGG CCTTTTGTCC GGCTCCTGCT TCCAGCATTG CAGCATCAGG
            541 CGGTACATCT CCTCGCTGCA GTTGTCTGGC CTCTCCATCC GGTGGCCGGT CTTCAGAAGG
            601 TTGAAGAGCC GCTCAGGAGG AATCCCAGGA TAGGGGTTTC CCCCTAGGGT CACGATCTCC
            661 CACAGCAGGA CACCAAAAGA CCATACATCA CTTTGCGTGG TGTAGATATG ATCAAAAAGG
            721 GATTCAATTG CCATCCATTT AACTGGGAAT CCGACCCTGG CTCCTCTTCA CGTAGGAATC
            781 CTCTTCATAA ACATCTCGGG ACAAGCCGAA ATCCGAAATC TTCATCTTCC GCCCCTCAGC
            841 TACCAGGATG TTTCTGGCTG CCAAAGTCCC GATGAACGAG CTTCATCTCG GCCAGATACT
            901 GCATCCCCTG TGAGATCTGC CAGGCAAATG AGATGAGGTC GCCCATGGTG AGGGCCCGCT
            961 CATCCGGGGT GGTCCAGGGA GCTGGAGTTG CGGGCTGCCT CCACTGCCCA GGTAGCCAGG
           1021 CCCCACTTTG CGGCTCTCGC CGAGGAGCCC CGGCAGGGAG CCGTAATTGG GCGTACTTCC
           1081 CACGATGAAG AAGGAGCGGG TCAGTCCTGG CTGCAAGGCC CCATACATTG GATTGACATG
           1141 TGGGTTGTGG ACTGCCTTCC AGAACGGTTG GAAACCCTCT TGACACGGCA CAGGTTCTCT
          1201 CGCAGAAAGC CCTTCA
          //
          Primer: SV40NLS_R
          Sequence file: RDB15115_A7Ffc.seq check
          >01163_15115_A7Ff_SV40NLS_R_B01_02_ABI08.ab1
              1 GAGAGAGGGG TAGCAGTAGC CTCATCATCA CTAGATGGCA TTTCTTCTGA GCAAAACAGG
             61 TTTTCCTCAT TAAAGGCATT CCACCACTGC TCCCATTCAT CAGTTCCATA GGTTGGAATC
            121 TAAAATACAC AAACAATTAG AATCAGTAGT TTAACACATT ATACACTTAA AAATTTTATA
            181 TTTACCTTAG AGCTTTAAAT CTCTGTAGGT AGTTTGTCCA ATTATGTCAC ACCACAGAAG
            241 TAAGGTTCCT TCACAAAGAT CCGGACCAAA GCGGCCATCG TGCCTCCCCA CTCCTGCAGT
            301 TCGGGGGCAT GGATGCGCGG ATAGCCGCTG CTGGTTTCCT GGATGCCGAC GGATTTGCAC
            361 TGCCGGTAGA ACTCCGCGAG GTCGTCCAGC CTCAGGCAGC AGCTGAACCA ACTCGCGAGG
            421 GGATCGAGCC CCAAGCTTAT GAGTCATGGT CAGAATCTAA GAAACAGAAA CATCTTGTAG
            481 CAGCATTAAA TTACAAACAC AGGAAGAGCT GATCCATGGG CCTTTCACAC AGGCTCTATA
            541 AGAGCCAAAC ACCATCTTTT TCACATCAGA ATTGTAATCA GAGGTGTAGC AGTCCTTGGT
            601 CATTGTCATT GCTACTTCTA ACACAGCTAG CTAGCCTGTC CCTTTATGAG TAAGTTGATA
            661 AGATTCAAAT TAAAGTGAGT ACAAGCCCTA CCTGCTCCTA ACAGGTAGGA TGAGCCACTG
            721 CTTTCGCCTC TACTTGGCTG TGGGGTGTGC TGCAGGCTGG TGGCAGGTGG GATTCACAGC
            781 AGCAAAGCCT CTGCTGTCAG CAATTCTGGG ACTCCAACAG CCCCCTTTCT TCATACTGTT
            841 CTCATCAGGG GTAGTGGCTG CTCAGTACCG AACACGTGAG GATGATGTGG TGGGCCCGGG
            901 GGACAAATGT GTCTTGTGAG CCCTCTTCCC TGATACCACC ATCCACATAG TAAAGATGGC
            961 ATGGAATCTC TCCATTGTGC TAAATCTAGG AATCATCTTA ATCATGATGA AAACCAAATG
           1021 TCTTCCTAAG GAAAGGTTGC ATGATTAAAA AAAAAATCAT TCTCAGATCT CGAAATGCTG
           1081 AAGCCCAGGC CATCCTCGGG GACCCTCATC CCCTCCAGTC TTGAGGGCAT GGAACACTAC
          1141 CCCAGGTCCC CCCCCGGCAA GTCCTCCTCC AATTTCTTGA ATATAG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Takahashi, M., Cloning and expression of the ret proto-oncogene encoding a tyrosine kinase with two potential transmembrane domains. Oncogene, 3, 571-578 (1988). PMID 3078962.
          reference Takahashi, M., Isolation of ret proto-oncogene cDNA with an amino-terminal signal sequence. Oncogene, 4, 805-806 (1989). PMID 2660074.
          reference Ishizaka, Y., Human ret proto-oncogene mapped to chromosome 10q11.2. Oncogene, 4, 1519-1521 (1989). PMID 2687772.
          user report Nakata T., Differential expression of multiple isoforms of the ELKS mRNAs involved in a papillary thyroid carcinoma. Gene. Chromosome. Canc., 35, 30-37 (2002). PMID 12203787.

          2022.02.09

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