Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSVtsA58 ori (-)

Plasmid clone of SV40 temperature sensitive large T antigen cDNA

Catalog number RDB01126
Resource name pSVtsA58 ori (-)
Alternative name SV40 ori (-) DNA cDNA
Clone info. Plasmid clone of SV40 temperature sensitive large T antigen cDNA.
Vector backbone pBR322 (Plasmid)
Size of vector backbone 4,361 bp
Selectable markers Amp^r
Gene/insert name SV40 temperature sensitive large T antigen cDNA
Depositor|Developer Ohno, Tadao |
Other clones in our bank
External Database
SV40 temperature sensitive large T antigen
            Reference sequence
              
             
            Remarks, protocol and/or map (png) RDB01126map_v2.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution Distributed with written consent of Depositor.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form             [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form by depositor [Word]
            Remarks ((Additional form)) Approval Form(FormD)
            Please obtain written approval of the depositor.
            提供案内 (日本国内) [open/close]

            提供条件 寄託者に許可をとること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            寄託者による提供承諾書 [Word]
            備考 【追加提供依頼書】承諾書(FormD)
            寄託者の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB01126 pSVtsA58 ori (-) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pSVtsA58 ori (-) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01126).

            Reference section:

            Ohno, T., Yamaguchi, N. , Life span elongation of Werner's syndrome fibroblasts by co-culture with origin-defective SV-40 DNA transformed cells. Human Genetics, 68, 209-210 (1984). PMID 6094336.

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18365_B0Gap1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: RDB08068_F1 (Pr0221)
            Region: AmpR pro
            Sequence file: RDB18365_B0Gaa.seq check
            >01126_18365_B0Ga_3_RDB08068_F1_F06_18_ABI24.ab1
                1 NNNNNNNNNN NNNNNNTTCN CNNNNNNTTT NNATTACCCT TAGAAAGCGG TCTGTGAAAA
               61 ACCCCTACCC NATTTCCTTT TTGTTAAGTG ACCTAATTAA CAGGAGGACA CAGAGGGTGG
              121 ATGGGCAGCC TATGATTGGA ATGTCCTCTC AAGTAGAGGA GGTTAGGGTT TATGAGGACA
              181 CAGAGGAGCT TCCTGGGGAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA
              241 GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT
              301 CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG
              361 TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA
              421 ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG
              481 TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT
              541 TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT
              601 GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG
              661 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA
              721 TATGTATCCG CTCATGANAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA
              781 GANTATGAGT ATTCAACATT TCCGNNNCGC CCTTATTCCC TTTTTTTGCG GCATTTTNGC
              841 NTTCCTGNTT TTTGCTCACC CAGAAACGCN NGNNAAAGTA AAAGANGCNN AANNNCANTN
            901 NGGNNGCNCN NNNNGNNNAC NTCNNANNNN NNNNNN
            //
            Primer: pGL4pA_R (Pr0664)
            Region: SV40 pA, insert 3'
            Sequence file: RDB18365_B0Gab.seq check
            >01126_18365_B0Ga_3_pGL4pA_R_G06_21_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN CNNNANGCGN NTCCTGCATT AGGNAGCAGC CCAGTAGTAG
               61 GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA
              121 CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC
              181 GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC
              241 ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCC AGACATGATA
              301 AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT
              361 TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT
              421 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA
              481 AGCAAGTAAA ACCTCTACAA ATGTGATATG GCTGATCATG AACAGACTGT GAGGACTGTG
              541 AGGGCTGAGG GGCCTGAAAT GAGCCTTGAG ACTGTGAATC AATGCCTGTT TCATGCCCTG
              601 AGTCTTCCAT GTTCTTCTCC CCACCATCTT CATTTTTATC AGCATTTTCC TGGCTGTCTT
              661 CATCATCATT ATCACTGTTT CTTAGCCAAT CTAAAACTCC AGTTCCCATA GCCACATTAA
              721 ACTTCATTTT TTGATACACT GACAAACTAA ACTCTTTGTC CAATCTCTCT TTCCACTCCA
              781 CNATTCTGCT CTGAATACTT TGANCAAACT CAGCCACAGG TCTGTACNNA ATTAACATAA
              841 GAAGCAAAGC AATGCCACTT TNAATTATCC TCTTTTCTAA CAAAAACTCA CTGCNNTCCN
            901 NNNNATGCTT TAAATAATCT TNGGNNNNNA ANNCNANTTN NTTTANNANN NNNNNNNN
            //
            Primer: LTA_M_F (Pr0270)
            Region: insert mid (Ala438Val)
            Sequence file: RDB18365_B0Gac.seq check
            >01126_18365_B0Ga_3_LTA_M_F_C01_07_ABI24.ab1
                1 NNNNNNNNNN NNNNNNCNNN NNGNTTNNNN NTCTGGCCTG NANNGTTTTA GGCACACTGT
               61 ACTCATTCAT GGNGACTATT CCAGGGGGAA ATATTTGAGT TCTTTTATTT AGGTGTTTCT
              121 TTTCTAAGTT TACCTTAACA CTGCCATCCA AATAATCCCT TAAATTGTCC AGGTTATTAA
              181 TTCCCTGACC TGAAGGCAAA TCTCTGGACT CCCCTCCAGT GCCCTTTACA TCCTCAAAAA
              241 CTACTAAAAA CTGGTCAATA GCTACTCCTA GCTCAAAGTT CAGCCTGTCC AAGGGCAAAT
              301 TAACATTTAA AGCTTTCCCC CCACATAATT CAAGCAAAAC AGCTGCTAAT GTAGTTTTAC
              361 CACTATCAAT TGGTCCTTTA AACAGCCAGT ATCTTTTTTT AGGAATGTTG TACACCATGC
              421 ATTTTAAAAA GTCATACACC ACTGAATCCA TTTTGGGCAA CAAACAGTGT AGCCAAGCAA
              481 CTCCAGCCAT CCATTCTTCT ATGTCAGCAG AGCCTGTAGA ACCAAACATT ATATCCATCC
              541 TATCCAAAAG ATCATTAAAT CTGTTTGTTA ACATTTGTTC TCTAGTTAGT TGTAGGCTAT
              601 CAACCCGCTT TTTAGCTAAA ACAGTATCAA CAGCCTGTTG GCATATGGTT TTTTGGTTTT
              661 TGCTGTCAGC AAATATAGCA GCATTTGCAT AATGCTTTTC ATGGTACTTA TAGTGGCTGG
              721 GCTGTTCTTT TTTAATACAT TTTTAAACAC ATTTCNAAAC TGTATTGAAA TTCCNAGNAC
              781 ATCNNNAGCA ATAACAACAC ATCATCACAT TTTNGTTTCC ATNNCATANT CNNNNACNNN
              841 CTTNNNNNNN ACTNNNTTAN NTTCCNNNGC TTCTNNNGGN NNNAAANCAT GCNNCNTNNN
            901 NNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Ohno, T., Life span elongation of Werner's syndrome fibroblasts by co-culture with origin-defective SV-40 DNA transformed cells. Human Genetics, 68, 209-210 (1984). PMID 6094336.
            user_report Fukumitsu K., Establishment of a cell line derived from a mouse fetal liver that has the characteristic to promote the hepatic maturation of mouse embryonic stem cells by a coculture method. Tissue Eng. Part A., 15, 3847-3856 (2009). PMID 19558317.
            user_report Takagi Y., Different initial steps of apoptosis induced by two types of antineoplastic drugs. Biochem. Pharmacol., 76, 303-311 (2008). PMID 18573489.
            user_report Takasaki I., Identification of genetic networks involved in the cell growth arrest and differentiation of a rat astrocyte cell line RCG-12. J. Cell. Biochem., 102, 1472-1485 (2007). PMID 17440958.
            user_report Tabuchi Y, Functional characterization of a conditionally immortalized mouse epididymis caput epithelial cell line MEPC5 using temperature-sensitive simian virus 40 large T-antigen. Biochem. Biophys. Res. Commun., 329, 812-823 (2005). PMID 15752729.
            user_report Takagi Y., Roles of MGMT and MLH1 proteins in alkylation-induced apoptosis and mutagenesis. DNA Repair (Amst)., 2, 1135-1146 (2003). PMID 13679151.

            2023.05.02

            GNP_filter3_RDBDEP_html_230501.pl