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pSVtsA58 ori (-)

Plasmid clone of SV40 temperature sensitive large T antigen cDNA

Catalog number RDB01126
Resource name pSVtsA58 ori (-)
Alternative name SV40 ori (-) DNA cDNA
Clone info. Plasmid clone of SV40 temperature sensitive large T antigen cDNA.
Vector backbone pBR322 (Plasmid)
Size of vector backbone 4,361 bp
Selectable markers Amp^r
Gene/insert name SV40 temperature sensitive large T antigen cDNA
Depositor Ohno, Tadao |

Distribution information

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RDB01126 pSVtsA58 ori (-) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01126.gif from Depositor

Featured content

References

original Ohno, T., Life span elongation of Werner's syndrome fibroblasts by co-culture with origin-defective SV-40 DNA transformed cells. Human Genetics, 68, 209-210 (1984). PMID 6094336.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12812_A4Fr.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR_BamHI_F2 RDB12812_A4Fra.seq
>01126_12812_A4Fr_pBR_BamHI_F2_G08_20.ab1
    1 GCACAACATG GCGACACACC CGTCCTGTGG ATCCCCAGGA AGCTCCTCTG TGTCCTCATA
   61 AACCCTAACC TCCTCTACTT GAGAGGACAT TCCAATCATA GGCTGCCCAT CCACCCTCTG
  121 TGTCCTCCTG TTAATTAGGT CACTTAACAA AAAGGAAATT GGGTAGGGGT TTTTCACAGA
  181 CCGCTTTCTA AGGGTAATTT TAAAATATCT GGGAAGTCCC TTCCACTGCT GTGTTCCAGA
  241 AGTGTTGGTA AACAGCCCAC AAATGTCAAC AGCAGAAACA TACAAGCTGT CAGCTTTGCA
  301 CAAGGGCCCA ACACCCTGCT CATCAAGCAG CACTGTGGTT GCTGTGTTAG TAATGTGCAA
  361 AACAGGAGGC ACATTTTCCC CACCTGTGTA GGTTCCAAAA TATCTAGTGT TTTCATTTTT
  421 ACTTGGATCA GGAACCCAGC ACTCCACTGG ATAAGCATTA TCCTTATCCA AAACAGCCTT
  481 GTGGTCAGTG TTCATCTGCT GACTGTCAAC TGTAGCATTT TTGGGGGTTA CAGTTTGAGC
  541 AGGATATTTG GTCCTGTAGT TTGCTAACAC ACCCTGCAGC TCCAAAGGTT CCCCACCAAC
  601 AGCAAAAAAA TGAAAGTTTG ACCCTTGTAT GGGTTTTCCA GCACCATTTT CATGAGTTTT
  661 TTGTGTCCCT GAATGCAAGT TTAACATAGC AGTTACCCCA ATAACCTCAG TTTTAACAGT
  721 AACAGCTTCC CACATCAAAA TATTTCCACA GGTTAAGTCC TCATTTAAAT TAGGCAAAGG
  781 AATTCTAGCC ACACTGTAGC AAGGCAGTTG GTCTTTGTCT GGAGAGTCAT CTGTAAACTG
  841 TTTTTCAGCT GCTAAGCTTT TACTTAAGCC TTTTTTGATG TTCATCAGGA TTGCCCATTT
  901 TGAGGATTTA AAAAGCACTC CACCCTCAGT GAAGCTGTCT ACTCCAGTTT TAACTCCCTA
  961 GAACTTCTAT TCCCTCCCTT TTATGACGAG CTTTGGGCAC TTTGCACTGG TTTCCTTTTG
 1021 GGTTTTTTTG GAGCTGCCCC TGGAACACTT TCTTTTTCCT TTTTGTTGGG GCCATCGTCA
 1081 TAGGCTTTTA GAGCAGAGTT ACACTCCGTA CAGGCTAGCA AGTAAAGGCA AACATTCCCA
1141 CCTTGA
//
Primer: pBR_BamHI_R2 RDB12812_A4Frb.seq
>01126_12812_A4Fr_pBR_BamHI_R2_H08_23.ab1
    1 GCTGCTGCGT AGTTACGAGC GTGAGCGGCA CGATGCGTCC GGCGTAGAGG ATCCAGACAT
   61 GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT
  121 TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA
  181 AGTTAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT
  241 TTAAAGCAAG TAAAACCTCT ACAAATGTGA TATGGCTGAT CATGAACAGA CTGTGAGGAC
  301 TGTGAGGGCT GAGGGGCCTG AAATGAGCCT TGAGACTGTG AATCAATGCC TGTTTCATGC
  361 CCTGAGTCTT CCATGTTCTT CTCCCCACCA TCTTCATTTT TATCAGCATT TTCCTGGCTG
  421 TCTTCATCAT CATTATCACT GTTTCTTAGC CAATCTAAAA CTCCAGTTCC CATAGCCACA
  481 TTAAACTTCA TTTTTTGATA CACTGACAAA CTAAACTCTT TGTCCAATCT CTCTTTCCAC
  541 TCCACAATTC TGCTCTGAAT ACTTTGAGCA AACTCAGCCA CAGGTCTGTA CCAAATTAAC
  601 ATAAGAAGCA AAGCAATGCC ACTTTGAATT ATCCTCTTTT CTAACAAAAA CTCACTGCGT
  661 TCCAGGCAAT GCTTTAAATA ATCTTTGGGC CTAAAATCTA TTTGTTTTAC AAATCTGGCC
  721 TGCAGTGTTT TAGGCACACT GTACTCATTC ATGGTGACTA TTCCAGGGGG AAATATTTGA
  781 GTTCTTTTAT TTAGGTGTTT CTTTTCTAAG TTTACCTTAA CACTGCCATC CAAATAATCC
  841 CTTAAATTGT CCAGGTTATT AATTCCCTGA CCTGAAGGCA AATCTCTGGA CTCCCCTCCA
  901 GTGCCCTTTA CATCCTCAAA AAACTACTAA AAAACTGGTC AAATAGCTAC TCCCTAGCTC
  961 AAAGTTTCAA GCCCTGTCCA AGGACAAATT TAAACATTTA AAAGCTTTCC CCCACATTAA
 1021 TTCAAGCAAA ACAGCTGGCT TAATGGTAGT TTTAACACTA TCATGGTTCC GTTAACCAGG
1081 CCAGT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Ohno, T., Life span elongation of Werner's syndrome fibroblasts by co-culture with origin-defective SV-40 DNA transformed cells. Human Genetics, 68, 209-210 (1984). PMID 6094336.
user report Fukumitsu K., Establishment of a cell line derived from a mouse fetal liver that has the characteristic to promote the hepatic maturation of mouse embryonic stem cells by a coculture method. Tissue Eng. Part A., 15, 3847-3856 (2009). PMID 19558317.
user report Takagi Y., Different initial steps of apoptosis induced by two types of antineoplastic drugs. Biochem. Pharmacol., 76, 303-311 (2008). PMID 18573489.
user report Takasaki I., Identification of genetic networks involved in the cell growth arrest and differentiation of a rat astrocyte cell line RCG-12. J. Cell. Biochem., 102, 1472-1485 (2007). PMID 17440958.
user report Tabuchi Y, Functional characterization of a conditionally immortalized mouse epididymis caput epithelial cell line MEPC5 using temperature-sensitive simian virus 40 large T-antigen. Biochem. Biophys. Res. Commun., 329, 812-823 (2005). PMID 15752729.
user report Takagi Y., Roles of MGMT and MLH1 proteins in alkylation-induced apoptosis and mutagenesis. DNA Repair (Amst)., 2, 1135-1146 (2003). PMID 13679151.

2018.12.11

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