Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSVtsA58 ori (-) (#RDB01126)

Plasmid clone of SV40 temperature sensitive large T antigen cDNA

Alternative name SV40 ori (-) DNA cDNA
Clone info. Plasmid clone of SV40 temperature sensitive large T antigen cDNA.
Vector backbone pBR322 (Plasmid)
Size of vector backbone 4,361 bp
Selectable markers Amp^r
Gene/insert name SV40 temperature sensitive large T antigen cDNA
Depositor|Developer Ohno, Tadao |
 
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form by depositor [Word]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution Distributed with written consent of Depositor.
Remarks ((Additional form)) Approval Form(FormD)
Please obtain written approval of the depositor.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
寄託者による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 寄託者に許可をとること。
備考 【追加提供依頼書】承諾書(FormD)
寄託者の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01126 pSVtsA58 ori (-) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pSVtsA58 ori (-) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01126).

Reference section:

Ohno, T., Yamaguchi, N. , Life span elongation of Werner's syndrome fibroblasts by co-culture with origin-defective SV-40 DNA transformed cells. Human Genetics, 68, 209-210 (1984). PMID 6094336. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18365_B0Gap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: RDB08068_F1 (Pr0221)
Region: AmpR pro
Sequence file: RDB18365_B0Gaa.seq check
>01126_18365_B0Ga_3_RDB08068_F1_F06_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTTCN CNNNNNNTTT NNATTACCCT TAGAAAGCGG TCTGTGAAAA
   61 ACCCCTACCC NATTTCCTTT TTGTTAAGTG ACCTAATTAA CAGGAGGACA CAGAGGGTGG
  121 ATGGGCAGCC TATGATTGGA ATGTCCTCTC AAGTAGAGGA GGTTAGGGTT TATGAGGACA
  181 CAGAGGAGCT TCCTGGGGAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA
  241 GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT
  301 CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG
  361 TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA
  421 ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG
  481 TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT
  541 TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT
  601 GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG
  661 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA
  721 TATGTATCCG CTCATGANAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA
  781 GANTATGAGT ATTCAACATT TCCGNNNCGC CCTTATTCCC TTTTTTTGCG GCATTTTNGC
  841 NTTCCTGNTT TTTGCTCACC CAGAAACGCN NGNNAAAGTA AAAGANGCNN AANNNCANTN
901 NGGNNGCNCN NNNNGNNNAC NTCNNANNNN NNNNNN
//
Primer: pGL4pA_R (Pr0664)
Region: SV40 pA, insert 3'
Sequence file: RDB18365_B0Gab.seq check
>01126_18365_B0Ga_3_pGL4pA_R_G06_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN CNNNANGCGN NTCCTGCATT AGGNAGCAGC CCAGTAGTAG
   61 GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA
  121 CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC
  181 GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC
  241 ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCC AGACATGATA
  301 AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT
  361 TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT
  421 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA
  481 AGCAAGTAAA ACCTCTACAA ATGTGATATG GCTGATCATG AACAGACTGT GAGGACTGTG
  541 AGGGCTGAGG GGCCTGAAAT GAGCCTTGAG ACTGTGAATC AATGCCTGTT TCATGCCCTG
  601 AGTCTTCCAT GTTCTTCTCC CCACCATCTT CATTTTTATC AGCATTTTCC TGGCTGTCTT
  661 CATCATCATT ATCACTGTTT CTTAGCCAAT CTAAAACTCC AGTTCCCATA GCCACATTAA
  721 ACTTCATTTT TTGATACACT GACAAACTAA ACTCTTTGTC CAATCTCTCT TTCCACTCCA
  781 CNATTCTGCT CTGAATACTT TGANCAAACT CAGCCACAGG TCTGTACNNA ATTAACATAA
  841 GAAGCAAAGC AATGCCACTT TNAATTATCC TCTTTTCTAA CAAAAACTCA CTGCNNTCCN
901 NNNNATGCTT TAAATAATCT TNGGNNNNNA ANNCNANTTN NTTTANNANN NNNNNNNN
//
Primer: LTA_M_F (Pr0270)
Region: insert mid (Ala438Val)
Sequence file: RDB18365_B0Gac.seq check
>01126_18365_B0Ga_3_LTA_M_F_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNNN NNGNTTNNNN NTCTGGCCTG NANNGTTTTA GGCACACTGT
   61 ACTCATTCAT GGNGACTATT CCAGGGGGAA ATATTTGAGT TCTTTTATTT AGGTGTTTCT
  121 TTTCTAAGTT TACCTTAACA CTGCCATCCA AATAATCCCT TAAATTGTCC AGGTTATTAA
  181 TTCCCTGACC TGAAGGCAAA TCTCTGGACT CCCCTCCAGT GCCCTTTACA TCCTCAAAAA
  241 CTACTAAAAA CTGGTCAATA GCTACTCCTA GCTCAAAGTT CAGCCTGTCC AAGGGCAAAT
  301 TAACATTTAA AGCTTTCCCC CCACATAATT CAAGCAAAAC AGCTGCTAAT GTAGTTTTAC
  361 CACTATCAAT TGGTCCTTTA AACAGCCAGT ATCTTTTTTT AGGAATGTTG TACACCATGC
  421 ATTTTAAAAA GTCATACACC ACTGAATCCA TTTTGGGCAA CAAACAGTGT AGCCAAGCAA
  481 CTCCAGCCAT CCATTCTTCT ATGTCAGCAG AGCCTGTAGA ACCAAACATT ATATCCATCC
  541 TATCCAAAAG ATCATTAAAT CTGTTTGTTA ACATTTGTTC TCTAGTTAGT TGTAGGCTAT
  601 CAACCCGCTT TTTAGCTAAA ACAGTATCAA CAGCCTGTTG GCATATGGTT TTTTGGTTTT
  661 TGCTGTCAGC AAATATAGCA GCATTTGCAT AATGCTTTTC ATGGTACTTA TAGTGGCTGG
  721 GCTGTTCTTT TTTAATACAT TTTTAAACAC ATTTCNAAAC TGTATTGAAA TTCCNAGNAC
  781 ATCNNNAGCA ATAACAACAC ATCATCACAT TTTNGTTTCC ATNNCATANT CNNNNACNNN
  841 CTTNNNNNNN ACTNNNTTAN NTTCCNNNGC TTCTNNNGGN NNNAAANCAT GCNNCNTNNN
901 NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ohno, T., Life span elongation of Werner's syndrome fibroblasts by co-culture with origin-defective SV-40 DNA transformed cells. Human Genetics, 68, 209-210 (1984). PMID 6094336. [link to RRC of NBRP]
user_report Fukumitsu K., Establishment of a cell line derived from a mouse fetal liver that has the characteristic to promote the hepatic maturation of mouse embryonic stem cells by a coculture method. Tissue Eng. Part A., 15, 3847-3856 (2009). PMID 19558317. [link to RRC of NBRP]
user_report Takagi Y., Different initial steps of apoptosis induced by two types of antineoplastic drugs. Biochem. Pharmacol., 76, 303-311 (2008). PMID 18573489. [link to RRC of NBRP]
user_report Takasaki I., Identification of genetic networks involved in the cell growth arrest and differentiation of a rat astrocyte cell line RCG-12. J. Cell. Biochem., 102, 1472-1485 (2007). PMID 17440958. [link to RRC of NBRP]
user_report Tabuchi Y, Functional characterization of a conditionally immortalized mouse epididymis caput epithelial cell line MEPC5 using temperature-sensitive simian virus 40 large T-antigen. Biochem. Biophys. Res. Commun., 329, 812-823 (2005). PMID 15752729. [link to RRC of NBRP]
user_report Takagi Y., Roles of MGMT and MLH1 proteins in alkylation-induced apoptosis and mutagenesis. DNA Repair (Amst)., 2, 1135-1146 (2003). PMID 13679151. [link to RRC of NBRP]